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The map label for this gene is nuoL [H]

Identifier: 108812302

GI number: 108812302

Start: 2419933

End: 2421777

Strand: Reverse

Name: nuoL [H]

Synonym: YPN_2140

Alternate gene names: 108812302

Gene position: 2421777-2419933 (Counterclockwise)

Preceding gene: 108812303

Following gene: 108812301

Centisome position: 53.41

GC content: 54.2

Gene sequence:

>1845_bases
ATGAACCTATTATATTTAACAATTCTGCTGCCACTGCTGGGCTTCTTGCTGTTGGCATTCTCCCGTGGGCGCTGGTCTGA
AAATACCGCGGCTACCGTTGGGGTGGGGTCTATCGGGCTGACTGCGCTGGTGACGTTGTATGTGGCGGTGGATTTCCTCA
GCCAAAAAGCCACTGGCGTACAGGTGTTTAATCAGACGCTGTGGAACTGGATGTCTGTTGGCACCTTTAACATTCCGCTG
ACCCTGACATTGGACGGTTTGTCGCTGACCATGTTGTCGGTGGTGACCGGTGTCGGCTTCCTGATCCACATGTATGCCTC
GTGGTATATGCGTGGGGAAGAGGGCTACTCTCGCTTCTTCGCCTATACCAACCTGTTTATCGCGAGCATGGTGGTTCTGG
TTCTGGCGGATAACCTGTTGCTGATGTATCTCGGCTGGGAAGGCGTGGGGCTGTGCAGTTATCTGCTGATCGGTTTCTAC
TACAAGCATTCTGCCAACGGTGCGGCGGCGATGAAAGCCTTTATCGTGACCCGTGTGGGTGACGTATTCCTGGCTTTTGC
GCTGTTCATTCTTTACCAAGAGTTGGGCACGCTGAACATCCGCGAACTGATGATTTTGGCACCGCAGAAACTGGAGATGG
GCTCGACCGCCATTACCTGGGCGACGCTAATGCTCTTGGGGGGGGCGGTCGGTAAATCAGCTCAGTTGCCGCTACAAACC
TGGTTGGCTGATGCGATGGCCGGCCCGACGCCGGTTTCCGCGCTGATCCATGCGGCGACCATGGTGACCGCCGGTGTCTA
TCTGATTGCCCGTACTCATGGCCTGTTCCTGATGGCCCCTGAAGTACTGCATTTAGTCGGGGTTATCGGTGCGGTCACAC
TGGTGTTGGCGGGCTTTGCCGCACTGGTGCAAACCGACATCAAGCGGGTGTTGGCGTACTCCACCATGAGCCAGATTGGC
TACATGTTTCTGGCGCTGGGCGTACAGGCGTGGGATGCGGCAATCTTCCATTTGATGACCCATGCCTTCTTTAAAGCGTT
ACTGTTCCTTTCCTCAGGCTCAGTTATTCTGGCCTGCCACCACGAGCAAAATATCTTCAAAATGGGCGGTCTGCGTAAGA
CCATTCCATTGGTCTATATCTGCTTCCTGGTGGGTGGTGCAGCATTGGCAGCCTTGCCAATCGTCACTGCCGGCTTCTAT
AGCAAAGACGAAATTCTGTGGGGCGCATTGGCCAGTGGCCATATCAATCTGATGGTAGCAGGGTTGGTCGGGGCATTCCT
CACCGCGCTGTATACTTTCCGGATGATTTTCATTGTGTTCCATGGTGAGCCAAAAACTAAAGCACATCCGGTTAAAGGGA
TCACCCATAGCCTGCCGTTAATCGTGTTACTGGTGCTGTCGACCTTTGTCGGTGCGTTGATAACACCACCACTGGCGGGT
GTGTTGCCCGAGCTTCACTTTGGTGACGAGGGCAAAATGCCGTTGGAAATTTTCTCCGGTGTCTTGGTGGTGATCGGTAT
TGCACTGGCCGCGTTCTTGTATCTGGGCAAACGCCAATTGGTTAACAGCATTGCGCAAAGTGCGCCGGGCCGCTTCTTTA
CGGTCTGGTGGTTCCATGCGTGGGGCTTCGACTGGTTGTACCATAACCTGTTTGTCCGCCCGTATCTGTGGATTGCAAAA
CTGTTGCAACGTGATCCGCTGAATTCACTGATGAATACACCGGCGGTGCTTTCGCGCTGGAGTAATCGCGGTCTGACCGT
CAGTGAGAATGGGCAGGTGCGTTGGTACGCCGCCTCTATAGGTTTGGGTGCAGTGATTGTGCTGGCTCTGTTGCTTTTCG
TGTAG

Upstream 100 bases:

>100_bases
CGCTGGCGGCAGCCGAGGCCAGTATTGGCCTGGCACTGCTGCTGCAGTTGTATCGTCGCCGCCATACTCTGGATATCGAT
ACAGTCAGTGAGATGCGCGG

Downstream 100 bases:

>100_bases
ATGCCTGAATAGGCTCATTAATTAGGGACACAAAACGCCATGCTATTACCTTGGCTAATTCTTATCCCCTTTATCGGCGG
TCTGCTGTGCTGGCAGCTTG

Product: NADH dehydrogenase subunit L

Products: NA

Alternate protein names: NADH dehydrogenase I subunit L; NDH-1 subunit L; NUO12 [H]

Number of amino acids: Translated: 614; Mature: 614

Protein sequence:

>614_residues
MNLLYLTILLPLLGFLLLAFSRGRWSENTAATVGVGSIGLTALVTLYVAVDFLSQKATGVQVFNQTLWNWMSVGTFNIPL
TLTLDGLSLTMLSVVTGVGFLIHMYASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFY
YKHSANGAAAMKAFIVTRVGDVFLAFALFILYQELGTLNIRELMILAPQKLEMGSTAITWATLMLLGGAVGKSAQLPLQT
WLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMAPEVLHLVGVIGAVTLVLAGFAALVQTDIKRVLAYSTMSQIG
YMFLALGVQAWDAAIFHLMTHAFFKALLFLSSGSVILACHHEQNIFKMGGLRKTIPLVYICFLVGGAALAALPIVTAGFY
SKDEILWGALASGHINLMVAGLVGAFLTALYTFRMIFIVFHGEPKTKAHPVKGITHSLPLIVLLVLSTFVGALITPPLAG
VLPELHFGDEGKMPLEIFSGVLVVIGIALAAFLYLGKRQLVNSIAQSAPGRFFTVWWFHAWGFDWLYHNLFVRPYLWIAK
LLQRDPLNSLMNTPAVLSRWSNRGLTVSENGQVRWYAASIGLGAVIVLALLLFV

Sequences:

>Translated_614_residues
MNLLYLTILLPLLGFLLLAFSRGRWSENTAATVGVGSIGLTALVTLYVAVDFLSQKATGVQVFNQTLWNWMSVGTFNIPL
TLTLDGLSLTMLSVVTGVGFLIHMYASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFY
YKHSANGAAAMKAFIVTRVGDVFLAFALFILYQELGTLNIRELMILAPQKLEMGSTAITWATLMLLGGAVGKSAQLPLQT
WLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMAPEVLHLVGVIGAVTLVLAGFAALVQTDIKRVLAYSTMSQIG
YMFLALGVQAWDAAIFHLMTHAFFKALLFLSSGSVILACHHEQNIFKMGGLRKTIPLVYICFLVGGAALAALPIVTAGFY
SKDEILWGALASGHINLMVAGLVGAFLTALYTFRMIFIVFHGEPKTKAHPVKGITHSLPLIVLLVLSTFVGALITPPLAG
VLPELHFGDEGKMPLEIFSGVLVVIGIALAAFLYLGKRQLVNSIAQSAPGRFFTVWWFHAWGFDWLYHNLFVRPYLWIAK
LLQRDPLNSLMNTPAVLSRWSNRGLTVSENGQVRWYAASIGLGAVIVLALLLFV
>Mature_614_residues
MNLLYLTILLPLLGFLLLAFSRGRWSENTAATVGVGSIGLTALVTLYVAVDFLSQKATGVQVFNQTLWNWMSVGTFNIPL
TLTLDGLSLTMLSVVTGVGFLIHMYASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFY
YKHSANGAAAMKAFIVTRVGDVFLAFALFILYQELGTLNIRELMILAPQKLEMGSTAITWATLMLLGGAVGKSAQLPLQT
WLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMAPEVLHLVGVIGAVTLVLAGFAALVQTDIKRVLAYSTMSQIG
YMFLALGVQAWDAAIFHLMTHAFFKALLFLSSGSVILACHHEQNIFKMGGLRKTIPLVYICFLVGGAALAALPIVTAGFY
SKDEILWGALASGHINLMVAGLVGAFLTALYTFRMIFIVFHGEPKTKAHPVKGITHSLPLIVLLVLSTFVGALITPPLAG
VLPELHFGDEGKMPLEIFSGVLVVIGIALAAFLYLGKRQLVNSIAQSAPGRFFTVWWFHAWGFDWLYHNLFVRPYLWIAK
LLQRDPLNSLMNTPAVLSRWSNRGLTVSENGQVRWYAASIGLGAVIVLALLLFV

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1009

COG function: function code CP; NADH:ubiquinone oxidoreductase subunit 5 (chain L)/Multisubunit Na+/H+ antiporter, MnhA subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=384, Percent_Identity=40.1041666666667, Blast_Score=260, Evalue=2e-69,
Organism=Escherichia coli, GI1788614, Length=604, Percent_Identity=79.635761589404, Blast_Score=957, Evalue=0.0,
Organism=Escherichia coli, GI1788829, Length=487, Percent_Identity=32.4435318275154, Blast_Score=177, Evalue=2e-45,
Organism=Escherichia coli, GI1788831, Length=487, Percent_Identity=28.1314168377823, Blast_Score=123, Evalue=3e-29,
Organism=Escherichia coli, GI1788827, Length=422, Percent_Identity=30.3317535545024, Blast_Score=122, Evalue=8e-29,
Organism=Escherichia coli, GI2367154, Length=352, Percent_Identity=29.5454545454545, Blast_Score=93, Evalue=4e-20,
Organism=Escherichia coli, GI1788613, Length=465, Percent_Identity=26.4516129032258, Blast_Score=86, Evalue=7e-18,
Organism=Escherichia coli, GI145693160, Length=269, Percent_Identity=24.907063197026, Blast_Score=67, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR001516
- InterPro:   IPR003945
- InterPro:   IPR018393 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF00662 Oxidored_q1_N [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 66860; Mature: 66860

Theoretical pI: Translated: 9.33; Mature: 9.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLLYLTILLPLLGFLLLAFSRGRWSENTAATVGVGSIGLTALVTLYVAVDFLSQKATGV
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHCCCHHH
QVFNQTLWNWMSVGTFNIPLTLTLDGLSLTMLSVVTGVGFLIHMYASWYMRGEEGYSRFF
HHHHHHHHHHHHCCEEECEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
AYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYKHSANGAAAMKAFIVTRVG
HHHHHHHHHHHHHHHHCCHHEEEECCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
DVFLAFALFILYQELGTLNIRELMILAPQKLEMGSTAITWATLMLLGGAVGKSAQLPLQT
HHHHHHHHHHHHHHHCCCCHHHHHHCCCCHHCCCCHHHHHHHHHHHHCCCCCCCCCCHHH
WLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMAPEVLHLVGVIGAVTLVLAGFA
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEECHHHHHHHHHHHHHHHHHHHHH
ALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFHLMTHAFFKALLFLSSGSVILACH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
HEQNIFKMGGLRKTIPLVYICFLVGGAALAALPIVTAGFYSKDEILWGALASGHINLMVA
CCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCHHHHHH
GLVGAFLTALYTFRMIFIVFHGEPKTKAHPVKGITHSLPLIVLLVLSTFVGALITPPLAG
HHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHH
VLPELHFGDEGKMPLEIFSGVLVVIGIALAAFLYLGKRQLVNSIAQSAPGRFFTVWWFHA
HCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHH
WGFDWLYHNLFVRPYLWIAKLLQRDPLNSLMNTPAVLSRWSNRGLTVSENGQVRWYAASI
CCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHCCCCEEEECCCCEEEEEECC
GLGAVIVLALLLFV
CHHHHHHHHHHHHC
>Mature Secondary Structure
MNLLYLTILLPLLGFLLLAFSRGRWSENTAATVGVGSIGLTALVTLYVAVDFLSQKATGV
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHCCCHHH
QVFNQTLWNWMSVGTFNIPLTLTLDGLSLTMLSVVTGVGFLIHMYASWYMRGEEGYSRFF
HHHHHHHHHHHHCCEEECEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
AYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYKHSANGAAAMKAFIVTRVG
HHHHHHHHHHHHHHHHCCHHEEEECCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
DVFLAFALFILYQELGTLNIRELMILAPQKLEMGSTAITWATLMLLGGAVGKSAQLPLQT
HHHHHHHHHHHHHHHCCCCHHHHHHCCCCHHCCCCHHHHHHHHHHHHCCCCCCCCCCHHH
WLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMAPEVLHLVGVIGAVTLVLAGFA
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEECHHHHHHHHHHHHHHHHHHHHH
ALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFHLMTHAFFKALLFLSSGSVILACH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
HEQNIFKMGGLRKTIPLVYICFLVGGAALAALPIVTAGFYSKDEILWGALASGHINLMVA
CCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCHHHHHH
GLVGAFLTALYTFRMIFIVFHGEPKTKAHPVKGITHSLPLIVLLVLSTFVGALITPPLAG
HHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHH
VLPELHFGDEGKMPLEIFSGVLVVIGIALAAFLYLGKRQLVNSIAQSAPGRFFTVWWFHA
HCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHH
WGFDWLYHNLFVRPYLWIAKLLQRDPLNSLMNTPAVLSRWSNRGLTVSENGQVRWYAASI
CCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHCCCCEEEECCCCEEEEEECC
GLGAVIVLALLLFV
CHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7690854; 9205837; 9278503 [H]