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The map label for this gene is nfo [H]

Identifier: 108797946

GI number: 108797946

Start: 1080534

End: 1081340

Strand: Direct

Name: nfo [H]

Synonym: Mmcs_0972

Alternate gene names: 108797946

Gene position: 1080534-1081340 (Clockwise)

Preceding gene: 108797945

Following gene: 108797948

Centisome position: 18.94

GC content: 69.27

Gene sequence:

>807_bases
TTGCGTCTGCTCGCGCTGGTTGGGGGTGTGTCGGCGGGCGCGCCTAGACTCGGCTGCGTGCTCATCGGTTCACATGTCCG
CAACGACGATCCCCTGGCCGCTGCCGAGGCCGACGGCGCCGACGTGGTGCAGTTCTTCCTCGGCGACCCGCAGAGCTGGA
AGAAACCCAAACCGCGCGAGGACGCCGAGGTGCTGAAGGCGTCGAGCATCCCGCTCTACGTGCATGCGCCGTACCTGATC
AACGTCGCGTCGGCGAACAACCGGGTGCGAATCCCGTCGCGCAAGATCCTGCAGGACACCTGCGATGCGGCCGCCGAGGT
GGGCGCCACCGCGGTGATCGTGCACGGCGGCCACGCCGACGACAAGGACATGGAGGCCGGGTTCGAGCGGTGGGTCAAGG
CGCTCGACCGGCTCGAGACCACGGTGCCCGTCTACCTGGAGAACACCGCCGGCGGCGAGCACGCGATGGCGCGCTACTTC
GACACCATCGGCCGGCTGTGGGACCACATCGGCGACTACGGCATCGGGTTCTGCCTCGACACCTGCCACGCGTGGGCGGC
CGGCGAGGCGCTGGTCGACGCCGTCTCGCGGATCAAGCGGATCACCGGCCGCATCGACCTCGTCCACTGCAACGACTCGC
GTGATGCCGCGGGTTCGGGGGCCGACCGGCACGCCAACTTCGGCAACGGCCAGATCGACCCGCAACTGCTGGTCGCGGTG
GTGCAGGCCGCCGACGCTCCGGTCATCTGCGAGACCTCCGACGACGGGCGGAAGGACGACATCGCGTTCCTGCGCGAGAA
CGTCTGA

Upstream 100 bases:

>100_bases
CATCGCCACGCACCTCGAACGCGCGGACATGGCCGGATGAACTCCTTCTCACCGGCGAGCAGACGCAAATGTCCCGGATT
TCGCGGCATTTCGGGGACAT

Downstream 100 bases:

>100_bases
GGGTCACACTTCGATCGCAAACAGGCGTCGAACGGCGGGTGGCGAAGCGGTTAACTAGGCTGAGTCGGGCCAGAACCGAC
TCCGAAAGGCCGATGTGTGT

Product: endonuclease IV

Products: NA

Alternate protein names: Endodeoxyribonuclease IV; Endonuclease IV [H]

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MRLLALVGGVSAGAPRLGCVLIGSHVRNDDPLAAAEADGADVVQFFLGDPQSWKKPKPREDAEVLKASSIPLYVHAPYLI
NVASANNRVRIPSRKILQDTCDAAAEVGATAVIVHGGHADDKDMEAGFERWVKALDRLETTVPVYLENTAGGEHAMARYF
DTIGRLWDHIGDYGIGFCLDTCHAWAAGEALVDAVSRIKRITGRIDLVHCNDSRDAAGSGADRHANFGNGQIDPQLLVAV
VQAADAPVICETSDDGRKDDIAFLRENV

Sequences:

>Translated_268_residues
MRLLALVGGVSAGAPRLGCVLIGSHVRNDDPLAAAEADGADVVQFFLGDPQSWKKPKPREDAEVLKASSIPLYVHAPYLI
NVASANNRVRIPSRKILQDTCDAAAEVGATAVIVHGGHADDKDMEAGFERWVKALDRLETTVPVYLENTAGGEHAMARYF
DTIGRLWDHIGDYGIGFCLDTCHAWAAGEALVDAVSRIKRITGRIDLVHCNDSRDAAGSGADRHANFGNGQIDPQLLVAV
VQAADAPVICETSDDGRKDDIAFLRENV
>Mature_268_residues
MRLLALVGGVSAGAPRLGCVLIGSHVRNDDPLAAAEADGADVVQFFLGDPQSWKKPKPREDAEVLKASSIPLYVHAPYLI
NVASANNRVRIPSRKILQDTCDAAAEVGATAVIVHGGHADDKDMEAGFERWVKALDRLETTVPVYLENTAGGEHAMARYF
DTIGRLWDHIGDYGIGFCLDTCHAWAAGEALVDAVSRIKRITGRIDLVHCNDSRDAAGSGADRHANFGNGQIDPQLLVAV
VQAADAPVICETSDDGRKDDIAFLRENV

Specific function: Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic sites (AP sites) to produce new 5'-ends that are base-free deoxyribose 5-phosphate residues. It preferentially attacks modified AP sites created by ble

COG id: COG0648

COG function: function code L; Endonuclease IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AP endonuclease 2 family [H]

Homologues:

Organism=Escherichia coli, GI1788483, Length=275, Percent_Identity=28, Blast_Score=66, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17531193, Length=273, Percent_Identity=24.5421245421245, Blast_Score=71, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018246
- InterPro:   IPR001719
- InterPro:   IPR013022
- InterPro:   IPR012307 [H]

Pfam domain/function: PF01261 AP_endonuc_2 [H]

EC number: =3.1.21.2 [H]

Molecular weight: Translated: 28733; Mature: 28733

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS00729 AP_NUCLEASE_F2_1 ; PS00730 AP_NUCLEASE_F2_2 ; PS00731 AP_NUCLEASE_F2_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLLALVGGVSAGAPRLGCVLIGSHVRNDDPLAAAEADGADVVQFFLGDPQSWKKPKPRE
CEEEEEECCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCC
DAEVLKASSIPLYVHAPYLINVASANNRVRIPSRKILQDTCDAAAEVGATAVIVHGGHAD
HHHHHHHCCCCEEEECCEEEEEECCCCEEECCHHHHHHHHHHHHHHCCCEEEEEECCCCC
DKDMEAGFERWVKALDRLETTVPVYLENTAGGEHAMARYFDTIGRLWDHIGDYGIGFCLD
CHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHH
TCHAWAAGEALVDAVSRIKRITGRIDLVHCNDSRDAAGSGADRHANFGNGQIDPQLLVAV
HHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
VQAADAPVICETSDDGRKDDIAFLRENV
HHHCCCCEEEECCCCCCHHHHHHHHCCC
>Mature Secondary Structure
MRLLALVGGVSAGAPRLGCVLIGSHVRNDDPLAAAEADGADVVQFFLGDPQSWKKPKPRE
CEEEEEECCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCC
DAEVLKASSIPLYVHAPYLINVASANNRVRIPSRKILQDTCDAAAEVGATAVIVHGGHAD
HHHHHHHCCCCEEEECCEEEEEECCCCEEECCHHHHHHHHHHHHHHCCCEEEEEECCCCC
DKDMEAGFERWVKALDRLETTVPVYLENTAGGEHAMARYFDTIGRLWDHIGDYGIGFCLD
CHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHH
TCHAWAAGEALVDAVSRIKRITGRIDLVHCNDSRDAAGSGADRHANFGNGQIDPQLLVAV
HHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
VQAADAPVICETSDDGRKDDIAFLRENV
HHHCCCCEEEECCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]