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The map label for this gene is vsr [H]

Identifier: 108797796

GI number: 108797796

Start: 904987

End: 905388

Strand: Reverse

Name: vsr [H]

Synonym: Mmcs_0818

Alternate gene names: 108797796

Gene position: 905388-904987 (Counterclockwise)

Preceding gene: 108797798

Following gene: 108797795

Centisome position: 15.87

GC content: 61.44

Gene sequence:

>402_bases
ATGTCCCGCCGCATGTCTGCGCAACTGTCGCAGAACACCGGAATCGAAATGCGGCTGAGATCTGCGCTCCATGCGCGCGG
ACTCCGATACCGGTTGCATCAACGCCCCCTTCCCCAGCATCGGCGCACCGCTGATATCGTCTTCCGCCGAGCCAAGGTGG
CGGTATACGTCGACGGTTGCTTCTGGCATGGATGTCCGGAGCATGCGACCTGGCCCAAACGAAATGCCGAGTTCTGGCGG
AACAAGATCGAGGCCAATGTCCGCCGCGACCGCGAAACCGACACCTCCCTAATCGAGGCCGGATGGACTCCGGTCCGTGT
TTGGGAGCATGAAGACGTCGACGCGACGGCTGACCGCATCGGCATACTCGTCAAGCACCGTACTGCCACTACGAAGACCT
AG

Upstream 100 bases:

>100_bases
AACAAGCACCCTCCCGGAAACAGCACCTCACCCTGTGCGGTCAGTACCGGCTTAGCACGCGTAGGTGCCGGCGCGGAAGA
GAATTACCACAACACCCGAG

Downstream 100 bases:

>100_bases
TGCGCGACCCGACCGCGTGTCCTTCGCTGACGCCTGCGCTGGGCCATCTAGGCTTTTCACAGCTTGTCGGTGTGGCCTGC
TAAGACAGACCCAACCGACT

Product: DNA mismatch endonuclease vsr

Products: DNA [C]

Alternate protein names: V.NaeI [H]

Number of amino acids: Translated: 133; Mature: 132

Protein sequence:

>133_residues
MSRRMSAQLSQNTGIEMRLRSALHARGLRYRLHQRPLPQHRRTADIVFRRAKVAVYVDGCFWHGCPEHATWPKRNAEFWR
NKIEANVRRDRETDTSLIEAGWTPVRVWEHEDVDATADRIGILVKHRTATTKT

Sequences:

>Translated_133_residues
MSRRMSAQLSQNTGIEMRLRSALHARGLRYRLHQRPLPQHRRTADIVFRRAKVAVYVDGCFWHGCPEHATWPKRNAEFWR
NKIEANVRRDRETDTSLIEAGWTPVRVWEHEDVDATADRIGILVKHRTATTKT
>Mature_132_residues
SRRMSAQLSQNTGIEMRLRSALHARGLRYRLHQRPLPQHRRTADIVFRRAKVAVYVDGCFWHGCPEHATWPKRNAEFWRN
KIEANVRRDRETDTSLIEAGWTPVRVWEHEDVDATADRIGILVKHRTATTKT

Specific function: May nick NaeI sequences that contain T:G mispairs resulting from m5C-deamination [H]

COG id: COG3727

COG function: function code L; DNA G:T-mismatch repair endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the vsr family [H]

Homologues:

Organism=Escherichia coli, GI1788270, Length=112, Percent_Identity=36.6071428571429, Blast_Score=68, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004603
- InterPro:   IPR011335 [H]

Pfam domain/function: PF03852 Vsr [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 15625; Mature: 15494

Theoretical pI: Translated: 11.33; Mature: 11.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRRMSAQLSQNTGIEMRLRSALHARGLRYRLHQRPLPQHRRTADIVFRRAKVAVYVDGC
CCCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHEEEEEEEECCE
FWHGCPEHATWPKRNAEFWRNKIEANVRRDRETDTSLIEAGWTPVRVWEHEDVDATADRI
EECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHCCCCEEEEECCCCCCCCHHHH
GILVKHRTATTKT
EEEEECCCCCCCC
>Mature Secondary Structure 
SRRMSAQLSQNTGIEMRLRSALHARGLRYRLHQRPLPQHRRTADIVFRRAKVAVYVDGC
CCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHEEEEEEEECCE
FWHGCPEHATWPKRNAEFWRNKIEANVRRDRETDTSLIEAGWTPVRVWEHEDVDATADRI
EECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHCCCCEEEEECCCCCCCCHHHH
GILVKHRTATTKT
EEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: DNA mismatch endonuclease vsr specifically recognizes a T-G mismatch in a special context related to the dcm methylation sequence and makes an incision 5' to the mismatched T residue [C]

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7698663 [H]