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The map label for this gene is ydiP [H]

Identifier: 108797795

GI number: 108797795

Start: 903716

End: 904873

Strand: Reverse

Name: ydiP [H]

Synonym: Mmcs_0817

Alternate gene names: 108797795

Gene position: 904873-903716 (Counterclockwise)

Preceding gene: 108797796

Following gene: 108797794

Centisome position: 15.86

GC content: 63.04

Gene sequence:

>1158_bases
GTGGGAAAGACGGTCGCGGGTTTGTTCGCCGGTATCGGCGGTCTGGAAGTGGGCCTGAGCCGGTCAGGCTGGGAGACCAC
CTTGCTGTGCGAGATCGATCCCGCAGCACGTGCAGTCCTCTCGCACCACATGCCCGACGTCGAGTTGCAGGACGATGTGC
GACGGCTCCGGGCCCTGCCTCAGAGCCTCGACCTCTTGGCAGCCGGTTTCCCCTGCCAGGATCTGTCCCAGGCGGGCCGG
ACACGTGGAATTGCTGGCAAGAATTCCGGCCTGGTAGGTGAGGTATTTCGATTGATCAAGCGGAAACAAGGACCGCGCTG
GCTGCTGCTCGAGAACGTCCCCTTCATGCTCCAGCTGGACCGGGGAGCAGCCATGCGACACCTCACCGAGACACTCAATG
ACCTGGGGTACCGGTGGGCATATCGGGTTGTCGACGCGAGAGCATTCGGCCTGCCTCAGCGACGGCACCGGGTGTTGATG
CTCGCTTCCCGTTCAGAGGACCCGCGACCCGTGATGTTCGGTCAGGACATCGAGGCCGTCGATGAACCTGGGCCGCTCAC
CGACTACCCATGTGGCTTCTACTGGACAGAAGGAGTCCGTGGTCTGGGGTGGGCCGTGGATGCTGTCCCCACGATCAAAG
GCGGTTCCACTATCGGAATCGCGAGTCCACCCGCGGTCTGGCTGAAAAGCGGAGAGATCGTGACACCGGGGTTGGTTGAC
GCCGAACGTCTTCAGGGTTTCGACGCAGGGTGGACCGAGCCGGCCGCAAGCTTGACCGGTTTCCGCGAAGGGCAGCGTTG
GAAGCTCGTTGGGAACGCGGTGAGCACACGAATGTCGGAGTGGGTGGGGTCGCGTCTTGACACAACAGTCGGAGATTCCT
GGCCAGAATCGACGCCGATCCGCGCGGGCGACAAGTGGCCACGCGCAGCTTGGGGCGACGCAGACGGGTCGCACACCGTA
CCTGTGTCGATGTGGCCGGTGCGAGATCCATATGAGCATCTCGAGGGCTATCTGCAGATGACCAAACCGTTGTCGGCCCG
AGCTGCCGCCGGCTTCCTCCGACGAGCCAGATCAGGATCGCTGCGCTTCGTTCCAGGATTCCTTGAGGATGTGGAAGCGC
ACCTGGCGCGTATGTCGGGAACACAGGAAGTAGCGTGA

Upstream 100 bases:

>100_bases
CGCGTGTCCTTCGCTGACGCCTGCGCTGGGCCATCTAGGCTTTTCACAGCTTGTCGGTGTGGCCTGCTAAGACAGACCCA
ACCGACTAACGAGGAGCAGC

Downstream 100 bases:

>100_bases
GAGGTCCATCCATCATCGAGCGCACCATGTCCATTTCCTACTCGATAGGGATCGACGGTCGCGCATGGCAGTATCACTCA
CGTAGTGATAGGCACTCGAA

Product: DNA-cytosine methyltransferase

Products: NA

Alternate protein names: M2.BsuMI; Cytosine-specific methyltransferase M2.BsuMI [H]

Number of amino acids: Translated: 385; Mature: 384

Protein sequence:

>385_residues
MGKTVAGLFAGIGGLEVGLSRSGWETTLLCEIDPAARAVLSHHMPDVELQDDVRRLRALPQSLDLLAAGFPCQDLSQAGR
TRGIAGKNSGLVGEVFRLIKRKQGPRWLLLENVPFMLQLDRGAAMRHLTETLNDLGYRWAYRVVDARAFGLPQRRHRVLM
LASRSEDPRPVMFGQDIEAVDEPGPLTDYPCGFYWTEGVRGLGWAVDAVPTIKGGSTIGIASPPAVWLKSGEIVTPGLVD
AERLQGFDAGWTEPAASLTGFREGQRWKLVGNAVSTRMSEWVGSRLDTTVGDSWPESTPIRAGDKWPRAAWGDADGSHTV
PVSMWPVRDPYEHLEGYLQMTKPLSARAAAGFLRRARSGSLRFVPGFLEDVEAHLARMSGTQEVA

Sequences:

>Translated_385_residues
MGKTVAGLFAGIGGLEVGLSRSGWETTLLCEIDPAARAVLSHHMPDVELQDDVRRLRALPQSLDLLAAGFPCQDLSQAGR
TRGIAGKNSGLVGEVFRLIKRKQGPRWLLLENVPFMLQLDRGAAMRHLTETLNDLGYRWAYRVVDARAFGLPQRRHRVLM
LASRSEDPRPVMFGQDIEAVDEPGPLTDYPCGFYWTEGVRGLGWAVDAVPTIKGGSTIGIASPPAVWLKSGEIVTPGLVD
AERLQGFDAGWTEPAASLTGFREGQRWKLVGNAVSTRMSEWVGSRLDTTVGDSWPESTPIRAGDKWPRAAWGDADGSHTV
PVSMWPVRDPYEHLEGYLQMTKPLSARAAAGFLRRARSGSLRFVPGFLEDVEAHLARMSGTQEVA
>Mature_384_residues
GKTVAGLFAGIGGLEVGLSRSGWETTLLCEIDPAARAVLSHHMPDVELQDDVRRLRALPQSLDLLAAGFPCQDLSQAGRT
RGIAGKNSGLVGEVFRLIKRKQGPRWLLLENVPFMLQLDRGAAMRHLTETLNDLGYRWAYRVVDARAFGLPQRRHRVLML
ASRSEDPRPVMFGQDIEAVDEPGPLTDYPCGFYWTEGVRGLGWAVDAVPTIKGGSTIGIASPPAVWLKSGEIVTPGLVDA
ERLQGFDAGWTEPAASLTGFREGQRWKLVGNAVSTRMSEWVGSRLDTTVGDSWPESTPIRAGDKWPRAAWGDADGSHTVP
VSMWPVRDPYEHLEGYLQMTKPLSARAAAGFLRRARSGSLRFVPGFLEDVEAHLARMSGTQEVA

Specific function: This methylase may recognize the double-stranded sequence CTCGAG, causing specific methylation on C-? on both strands, and protects the DNA from cleavage by the BsuMI endonuclease [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the C5-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1788271, Length=199, Percent_Identity=29.1457286432161, Blast_Score=69, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525 [H]

Pfam domain/function: PF00145 DNA_methylase [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 42120; Mature: 41989

Theoretical pI: Translated: 7.20; Mature: 7.20

Prosite motif: PS00094 C5_MTASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKTVAGLFAGIGGLEVGLSRSGWETTLLCEIDPAARAVLSHHMPDVELQDDVRRLRALP
CCCHHHHHHHCCCCCEECCCCCCCCEEEEEEECHHHHHHHHHCCCCCCHHHHHHHHHHCH
QSLDLLAAGFPCQDLSQAGRTRGIAGKNSGLVGEVFRLIKRKQGPRWLLLENVPFMLQLD
HHHHHHHCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCEEEEEC
RGAAMRHLTETLNDLGYRWAYRVVDARAFGLPQRRHRVLMLASRSEDPRPVMFGQDIEAV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCEEEEEECCCCCCCCEEECCCCHHC
DEPGPLTDYPCGFYWTEGVRGLGWAVDAVPTIKGGSTIGIASPPAVWLKSGEIVTPGLVD
CCCCCCCCCCCCCEECCCCCCCCEECCCCCCCCCCCEECCCCCCCEEEECCCEECCCCCC
AERLQGFDAGWTEPAASLTGFREGQRWKLVGNAVSTRMSEWVGSRLDTTVGDSWPESTPI
HHHHCCCCCCCCCHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
RAGDKWPRAAWGDADGSHTVPVSMWPVRDPYEHLEGYLQMTKPLSARAAAGFLRRARSGS
CCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCC
LRFVPGFLEDVEAHLARMSGTQEVA
EEECCHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
GKTVAGLFAGIGGLEVGLSRSGWETTLLCEIDPAARAVLSHHMPDVELQDDVRRLRALP
CCHHHHHHHCCCCCEECCCCCCCCEEEEEEECHHHHHHHHHCCCCCCHHHHHHHHHHCH
QSLDLLAAGFPCQDLSQAGRTRGIAGKNSGLVGEVFRLIKRKQGPRWLLLENVPFMLQLD
HHHHHHHCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCEEEEEC
RGAAMRHLTETLNDLGYRWAYRVVDARAFGLPQRRHRVLMLASRSEDPRPVMFGQDIEAV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCEEEEEECCCCCCCCEEECCCCHHC
DEPGPLTDYPCGFYWTEGVRGLGWAVDAVPTIKGGSTIGIASPPAVWLKSGEIVTPGLVD
CCCCCCCCCCCCCEECCCCCCCCEECCCCCCCCCCCEECCCCCCCEEEECCCEECCCCCC
AERLQGFDAGWTEPAASLTGFREGQRWKLVGNAVSTRMSEWVGSRLDTTVGDSWPESTPI
HHHHCCCCCCCCCHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
RAGDKWPRAAWGDADGSHTVPVSMWPVRDPYEHLEGYLQMTKPLSARAAAGFLRRARSGS
CCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCC
LRFVPGFLEDVEAHLARMSGTQEVA
EEECCHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9455482; 9384377 [H]