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The map label for this gene is prpB [C]

Identifier: 108797236

GI number: 108797236

Start: 296261

End: 297019

Strand: Direct

Name: prpB [C]

Synonym: Mmcs_0256

Alternate gene names: 108797236

Gene position: 296261-297019 (Clockwise)

Preceding gene: 108797230

Following gene: 108797238

Centisome position: 5.19

GC content: 71.67

Gene sequence:

>759_bases
GTGCCCAACCACGATCTGAAGCAGCTCGCCGACGCCCTGCTCGCCCTGCACCAGCCCGGAAATCCCGTGGTCCTGCCCAC
CGTCTGGGATGCCTGGTCGGCCAACCTCGCCGTCGGCGCGGGGTTTGCCGCGCTCACGGTCGGCAGCCACCCGGTCGCCG
ATTCGGTCGGGCGCGCCGACAACGAGGGGATGACGTTCGACGAATTGCTCACCCGCGTCGCGCAGATCACCGATGCCGTC
GACGTACCGGTGTCGGTAGACATCGAATCGGGGTACGGCGAGGCGCCGACCCGGCTGATCGAGGGGCTGCTGGGCGTCGG
CGCCGTCGGGCTCAACATCGAGGACACCGTGCACGGTGAGGGTGGCCGGCTGCGGTCGGCCGGTGAACACGCCGAACTGG
TCGGGGCGCTGCGGGCGGCCGCCGACGACGCCGGGGTTCACGTGGTGGTCAACGCCCGCACCGACCTGTTCCTGCGCAAG
GACGGTGACGACGCCGACCGGGTGGACCGGGCGGTGGCGCGGCTGAAGGAGGCCGCCGAGGCGGGCGCCGACGTGCTCTA
CCCGGTCGGGCGCCACGATCCGGACACGCAACGCCGCCTGACCTCCGAGCTGCCGTTGCCGGTCAACGCGATCGGCATAC
CCGATCAGGACGATCCGGCGTCGTTCGGACCCCTCGGCGTCGCGCGCATCAGCTTCGGCCCGTTCCTCCAGGCGGCGCTG
GCCACCCGGGCGAACGAACTACTCGCCCGCTGGAAGTAG

Upstream 100 bases:

>100_bases
TGGGCTGAAGGGTGTCGTGAATCACAGTACGCAACCGATGCTAGCCACATCACTCACTTAAGAGTGAGACAGATCACGTC
TGAGGCGGCAGGATGGGCCC

Downstream 100 bases:

>100_bases
CGGCGCCGCCCTCGCCAGACAGCAGTCAGGGTCGTGAACCACGAGGATTCACGACCCTGACCTCATTCTCGGCGCCCAGA
GGGTTGCGCCGAGGGGCTAC

Product: hypothetical protein

Products: Pyruvate; Succinate [C]

Alternate protein names: NA

Number of amino acids: Translated: 252; Mature: 251

Protein sequence:

>252_residues
MPNHDLKQLADALLALHQPGNPVVLPTVWDAWSANLAVGAGFAALTVGSHPVADSVGRADNEGMTFDELLTRVAQITDAV
DVPVSVDIESGYGEAPTRLIEGLLGVGAVGLNIEDTVHGEGGRLRSAGEHAELVGALRAAADDAGVHVVVNARTDLFLRK
DGDDADRVDRAVARLKEAAEAGADVLYPVGRHDPDTQRRLTSELPLPVNAIGIPDQDDPASFGPLGVARISFGPFLQAAL
ATRANELLARWK

Sequences:

>Translated_252_residues
MPNHDLKQLADALLALHQPGNPVVLPTVWDAWSANLAVGAGFAALTVGSHPVADSVGRADNEGMTFDELLTRVAQITDAV
DVPVSVDIESGYGEAPTRLIEGLLGVGAVGLNIEDTVHGEGGRLRSAGEHAELVGALRAAADDAGVHVVVNARTDLFLRK
DGDDADRVDRAVARLKEAAEAGADVLYPVGRHDPDTQRRLTSELPLPVNAIGIPDQDDPASFGPLGVARISFGPFLQAAL
ATRANELLARWK
>Mature_251_residues
PNHDLKQLADALLALHQPGNPVVLPTVWDAWSANLAVGAGFAALTVGSHPVADSVGRADNEGMTFDELLTRVAQITDAVD
VPVSVDIESGYGEAPTRLIEGLLGVGAVGLNIEDTVHGEGGRLRSAGEHAELVGALRAAADDAGVHVVVNARTDLFLRKD
GDDADRVDRAVARLKEAAEAGADVLYPVGRHDPDTQRRLTSELPLPVNAIGIPDQDDPASFGPLGVARISFGPFLQAALA
TRANELLARWK

Specific function: Catalyzes The Formation Of Pyruvate And Succinate From 2-Methylisocitrate (By Similarity). [C]

COG id: COG2513

COG function: function code G; PEP phosphonomutase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786525, Length=216, Percent_Identity=29.6296296296296, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR015813 [H]

Pfam domain/function: NA

EC number: 4.1.3.30 [C]

Molecular weight: Translated: 26430; Mature: 26299

Theoretical pI: Translated: 4.47; Mature: 4.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
0.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNHDLKQLADALLALHQPGNPVVLPTVWDAWSANLAVGAGFAALTVGSHPVADSVGRAD
CCCHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCEEECCCEEEEEECCCCCHHHHCCCC
NEGMTFDELLTRVAQITDAVDVPVSVDIESGYGEAPTRLIEGLLGVGAVGLNIEDTVHGE
CCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHCCEECCCCCCCCCCC
GGRLRSAGEHAELVGALRAAADDAGVHVVVNARTDLFLRKDGDDADRVDRAVARLKEAAE
CCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCEEEEECCCCCHHHHHHHHHHHHHHHH
AGADVLYPVGRHDPDTQRRLTSELPLPVNAIGIPDQDDPASFGPLGVARISFGPFLQAAL
CCCCEEEECCCCCCHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCEEEHHHHHHHHHHHH
ATRANELLARWK
HHHHHHHHHCCC
>Mature Secondary Structure 
PNHDLKQLADALLALHQPGNPVVLPTVWDAWSANLAVGAGFAALTVGSHPVADSVGRAD
CCHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCEEECCCEEEEEECCCCCHHHHCCCC
NEGMTFDELLTRVAQITDAVDVPVSVDIESGYGEAPTRLIEGLLGVGAVGLNIEDTVHGE
CCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHCCEECCCCCCCCCCC
GGRLRSAGEHAELVGALRAAADDAGVHVVVNARTDLFLRKDGDDADRVDRAVARLKEAAE
CCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCEEEEECCCCCHHHHHHHHHHHHHHHH
AGADVLYPVGRHDPDTQRRLTSELPLPVNAIGIPDQDDPASFGPLGVARISFGPFLQAAL
CCCCEEEECCCCCCHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCEEEHHHHHHHHHHHH
ATRANELLARWK
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: methylisocitrate [C]

Specific reaction: methylisocitrate <==> Pyruvate + Succinate [C]

General reaction: Elimination [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]