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The map label for this gene is nasB [H]

Identifier: 108797238

GI number: 108797238

Start: 297720

End: 300362

Strand: Direct

Name: nasB [H]

Synonym: Mmcs_0258

Alternate gene names: 108797238

Gene position: 297720-300362 (Clockwise)

Preceding gene: 108797236

Following gene: 108797239

Centisome position: 5.22

GC content: 68.67

Gene sequence:

>2643_bases
ATGGGGCCTTCACACGAGGCGACATCGCGGCAATATACGATTCCTAGCCTTGCGCCCATGTCGACCAGCATTTCCAACGG
ACCAGCGAGAAACGTCGTGGTCGTCGGCCACGGCATGGTCGGGCACCGGTTCGTCGAAGCGCTGCGCGCGCGCGACGACG
CGGATGCCTGGCGGATCACCGTGCTCGCCGAGGAGACCGACGCCGCCTATGACCGAGTCGGTCTGACCGGCTACACCGAG
CACTGGGACCGTGCGCTGCTGGCGCTGGCCGGCAACGACTACGCCGGCGACGACCTGGTCGACCTGCGACTGGGACAGCG
GGTCACGTCGATCGACCGCGCCGCGCGCACGGTGAAGACCGCCGACGGAACCGCGTTCGCCTACGACGCGCTGGTGCTGG
CCACCGGGTCGTACGCCTTCGTGCCGCCGGTGCCGGGACGTGACCTGCCCGCCTGTCACGTCTACCGCACGTTCGACGAC
CTCGACGCGATCCGCGCCGACGCGGCACGGGCCCGCGAGACGAGCCGCGCACCGGTGGGTGTCGTCATCGGTGGCGGTCT
GCTGGGTCTGGAAGCCGCGAACGCGCTGCGCGGGTTCGGGCTGACCGCCCACGTCGTGGAGCGGTCGCCGCGGCTGATGT
CACAGCAGCTCGACGACGCCGGGGGTGCGTTGTTGAGCCGGATGATCGCCGACCTGGGCATCGAGGTCCACCTCGACGTC
AGCACCGACGCGATCACACCGGCGCAGCGCAACCGGCCCCTGCGGCGTTCCGAGGAGGACGACTCGCTTCGGGTGATGCT
GGGCGACGGGTCGTTCATCGACGCGGGCGTCGTGGTCTTCGCGGCCGGGGTCCGGCCGCGCGACGAACTCGCCCGCGACG
CCGGGCTCGTGATCGCCGAACGTGGTGGTGTGCTCACCGACCTCGGTTGCAAGACAAGCGATCCCAGCATCTACGCGGTC
GGTGAGGTGGCCGCCATCGAGGGCCGCTGCTACGGGCTGGTGGGTCCGGGCTACACCAGCGCGGAGGTGGTGGCCGACCG
GCTGCTCGGCGGTGCCGCGGAGTTCCCCGAGGCCGACATGTCGACCAAGCTCAAACTGCTCGGCGTCGACGTGGCCAGCT
TCGGCGATGCGATGGGACGCACCCCGGACTGCCTCGAGGTGGTCGTCAACGACGCGGTCAACCAGACCTACGCCAAGCTG
GTGCTCTCCGACGACGCCAAGACGCTGCTCGGCGGGGTGCTGGTGGGGGACGCCTCGGCGTACGGCGTGCTGCGGCCTAT
GGTCGGCGAACAACTGCCGGGCGACCCGATGGCGCTGATCGCGCCCGCCGGTTCCGGTGGAGCCGACGGTGGGCTCGGTG
TCGGCGCACTGCCGGCGGCTGCGCAGATCTGCTCGTGCAACAACGTCACCAAGGGTGATGTCACCGACGCGATCGCCGGC
GGATGCTGCGACGTGCCGGGCCTCAAGGCGTGCACCAAGGCGGGTACGTCGTGTGGATCGTGTGTCCCGCTGCTCAAACA
ACTGCTCGAAGCGGAGGGGGTCGAGCAGTCGAAGGCGTTGTGCGAGCACTTCTCTCACTCACGCGCCGAACTCTTCGAGA
TCGTGTCGGCGACGGAGATCCGCACGTTCTCCGGACTGCTCGAGCGCTTCGGAACCGGAAAGGGGTGCGACATCTGCAAA
CCCACCGTCGCCTCGATCCTTGCCTCGACCAGTTCCGAGCACATCCTCGACGGCGAGCAGGCCGCGCTGCAGGACTCCAA
CGACCACTTCCTGGCCAACATCCAACGCAACGGCAGCTATTCGGTGGTGCCACGCGTGCCCGGCGGTGACATCACTCCCG
AACAGTTGATCCTGATCGGCGAGATCGCCCGCGACTTCGACCTCTACACCAAGATCACCGGCGGCCAGCGCATCGATCTG
TTCGGCGCCCGCGTCGAGCAGCTCCCGGAGATCTGGCGGCGGCTCGTCGAGGGCGGGATGGAATCGGGCCACGCCTACGG
CAAGGCGCTGCGCACCGTGAAGAGCTGCGTCGGCAGCGACTGGTGCCGCTACGGCCAACAGGATTCGGTGCAGATGGCGA
TCAACCTGGAACTGCGCTACCGCGGGCTGCGGGCACCGCACAAGATCAAGATGGGCGTGTCCGGCTGCGCCCGCGAATGC
GCCGAGGCGCGCGGCAAGGATGTCGGCGTCATCGCCACCGAGCACGGCTGGAACCTGTACGTCGGCGGCAACGGCGGGAT
GACCCCCAAACACGCCCAGCTGCTCGCCGGTGACCTCGACGACGAGACGCTGATCCGCTACGTCGACCGGTTCCTGATGT
TCTACATCCGCACCGCGGACCGCCTTCAGCGCACCGCGCCGTGGGTCGAGCAGCTCGGACTGGACCACCTGCGGGAGGTC
GTCTGCGACGACTCCCTGGGCCTGGCAAGCGAATTCGAGGCGGCCATGGCCCGCCACGTCGAGGGCTACGCCTGCGAGTG
GAAGGGCGTGCTCGAAGACCCGGACAAGCTCTCCCGGTTCGTGTCCTTCGTCAACGCGCCCGATGTGCCCGACCCGACCG
TCACCTTCACCGAGAACGCCGGCCGCAAGGTGCCGGTGCCCATCGGGATGCCATCCATCCGACCGCTTCAGGAGACACCG
TGA

Upstream 100 bases:

>100_bases
ACTTGAGTCGACCCCGCTCATGTTCCGCTGTGGTGTTCGCCTCGGGCGAACAGCGGCGGCGGTCTCACAACCGCTCGCGA
AGCGACGTGAGCACACCGTG

Downstream 100 bases:

>100_bases
CCCTGCTCGACGACCGCAACGAGCTCAACGGGCCGCCGGATACGCGTTGGACGGTGGCCTGCCGCTACGACTACCTCATC
CCGTGCCGCGGCGTCGGGGT

Product: nitrite reductase (NAD(P)H) large subunit, NirB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 880; Mature: 879

Protein sequence:

>880_residues
MGPSHEATSRQYTIPSLAPMSTSISNGPARNVVVVGHGMVGHRFVEALRARDDADAWRITVLAEETDAAYDRVGLTGYTE
HWDRALLALAGNDYAGDDLVDLRLGQRVTSIDRAARTVKTADGTAFAYDALVLATGSYAFVPPVPGRDLPACHVYRTFDD
LDAIRADAARARETSRAPVGVVIGGGLLGLEAANALRGFGLTAHVVERSPRLMSQQLDDAGGALLSRMIADLGIEVHLDV
STDAITPAQRNRPLRRSEEDDSLRVMLGDGSFIDAGVVVFAAGVRPRDELARDAGLVIAERGGVLTDLGCKTSDPSIYAV
GEVAAIEGRCYGLVGPGYTSAEVVADRLLGGAAEFPEADMSTKLKLLGVDVASFGDAMGRTPDCLEVVVNDAVNQTYAKL
VLSDDAKTLLGGVLVGDASAYGVLRPMVGEQLPGDPMALIAPAGSGGADGGLGVGALPAAAQICSCNNVTKGDVTDAIAG
GCCDVPGLKACTKAGTSCGSCVPLLKQLLEAEGVEQSKALCEHFSHSRAELFEIVSATEIRTFSGLLERFGTGKGCDICK
PTVASILASTSSEHILDGEQAALQDSNDHFLANIQRNGSYSVVPRVPGGDITPEQLILIGEIARDFDLYTKITGGQRIDL
FGARVEQLPEIWRRLVEGGMESGHAYGKALRTVKSCVGSDWCRYGQQDSVQMAINLELRYRGLRAPHKIKMGVSGCAREC
AEARGKDVGVIATEHGWNLYVGGNGGMTPKHAQLLAGDLDDETLIRYVDRFLMFYIRTADRLQRTAPWVEQLGLDHLREV
VCDDSLGLASEFEAAMARHVEGYACEWKGVLEDPDKLSRFVSFVNAPDVPDPTVTFTENAGRKVPVPIGMPSIRPLQETP

Sequences:

>Translated_880_residues
MGPSHEATSRQYTIPSLAPMSTSISNGPARNVVVVGHGMVGHRFVEALRARDDADAWRITVLAEETDAAYDRVGLTGYTE
HWDRALLALAGNDYAGDDLVDLRLGQRVTSIDRAARTVKTADGTAFAYDALVLATGSYAFVPPVPGRDLPACHVYRTFDD
LDAIRADAARARETSRAPVGVVIGGGLLGLEAANALRGFGLTAHVVERSPRLMSQQLDDAGGALLSRMIADLGIEVHLDV
STDAITPAQRNRPLRRSEEDDSLRVMLGDGSFIDAGVVVFAAGVRPRDELARDAGLVIAERGGVLTDLGCKTSDPSIYAV
GEVAAIEGRCYGLVGPGYTSAEVVADRLLGGAAEFPEADMSTKLKLLGVDVASFGDAMGRTPDCLEVVVNDAVNQTYAKL
VLSDDAKTLLGGVLVGDASAYGVLRPMVGEQLPGDPMALIAPAGSGGADGGLGVGALPAAAQICSCNNVTKGDVTDAIAG
GCCDVPGLKACTKAGTSCGSCVPLLKQLLEAEGVEQSKALCEHFSHSRAELFEIVSATEIRTFSGLLERFGTGKGCDICK
PTVASILASTSSEHILDGEQAALQDSNDHFLANIQRNGSYSVVPRVPGGDITPEQLILIGEIARDFDLYTKITGGQRIDL
FGARVEQLPEIWRRLVEGGMESGHAYGKALRTVKSCVGSDWCRYGQQDSVQMAINLELRYRGLRAPHKIKMGVSGCAREC
AEARGKDVGVIATEHGWNLYVGGNGGMTPKHAQLLAGDLDDETLIRYVDRFLMFYIRTADRLQRTAPWVEQLGLDHLREV
VCDDSLGLASEFEAAMARHVEGYACEWKGVLEDPDKLSRFVSFVNAPDVPDPTVTFTENAGRKVPVPIGMPSIRPLQETP
>Mature_879_residues
GPSHEATSRQYTIPSLAPMSTSISNGPARNVVVVGHGMVGHRFVEALRARDDADAWRITVLAEETDAAYDRVGLTGYTEH
WDRALLALAGNDYAGDDLVDLRLGQRVTSIDRAARTVKTADGTAFAYDALVLATGSYAFVPPVPGRDLPACHVYRTFDDL
DAIRADAARARETSRAPVGVVIGGGLLGLEAANALRGFGLTAHVVERSPRLMSQQLDDAGGALLSRMIADLGIEVHLDVS
TDAITPAQRNRPLRRSEEDDSLRVMLGDGSFIDAGVVVFAAGVRPRDELARDAGLVIAERGGVLTDLGCKTSDPSIYAVG
EVAAIEGRCYGLVGPGYTSAEVVADRLLGGAAEFPEADMSTKLKLLGVDVASFGDAMGRTPDCLEVVVNDAVNQTYAKLV
LSDDAKTLLGGVLVGDASAYGVLRPMVGEQLPGDPMALIAPAGSGGADGGLGVGALPAAAQICSCNNVTKGDVTDAIAGG
CCDVPGLKACTKAGTSCGSCVPLLKQLLEAEGVEQSKALCEHFSHSRAELFEIVSATEIRTFSGLLERFGTGKGCDICKP
TVASILASTSSEHILDGEQAALQDSNDHFLANIQRNGSYSVVPRVPGGDITPEQLILIGEIARDFDLYTKITGGQRIDLF
GARVEQLPEIWRRLVEGGMESGHAYGKALRTVKSCVGSDWCRYGQQDSVQMAINLELRYRGLRAPHKIKMGVSGCARECA
EARGKDVGVIATEHGWNLYVGGNGGMTPKHAQLLAGDLDDETLIRYVDRFLMFYIRTADRLQRTAPWVEQLGLDHLREVV
CDDSLGLASEFEAAMARHVEGYACEWKGVLEDPDKLSRFVSFVNAPDVPDPTVTFTENAGRKVPVPIGMPSIRPLQETP

Specific function: Nitrate assimilation (denitrification); second step. [C]

COG id: COG1251

COG function: function code C; NAD(P)H-nitrite reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Homo sapiens, GI65787454, Length=303, Percent_Identity=23.7623762376238, Blast_Score=72, Evalue=3e-12,
Organism=Homo sapiens, GI226437568, Length=303, Percent_Identity=23.7623762376238, Blast_Score=72, Evalue=3e-12,
Organism=Homo sapiens, GI21389617, Length=303, Percent_Identity=23.7623762376238, Blast_Score=71, Evalue=4e-12,
Organism=Escherichia coli, GI1789765, Length=831, Percent_Identity=50.1805054151625, Blast_Score=823, Evalue=0.0,
Organism=Escherichia coli, GI1789065, Length=344, Percent_Identity=28.4883720930233, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1788892, Length=300, Percent_Identity=24.6666666666667, Blast_Score=70, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17559934, Length=296, Percent_Identity=22.972972972973, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24585130, Length=295, Percent_Identity=22.3728813559322, Blast_Score=73, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007419
- InterPro:   IPR013027
- InterPro:   IPR005117
- InterPro:   IPR012744
- InterPro:   IPR012748
- InterPro:   IPR017881
- InterPro:   IPR006067
- InterPro:   IPR006066
- InterPro:   IPR001327
- InterPro:   IPR017941 [H]

Pfam domain/function: PF04324 Fer2_BFD; PF01077 NIR_SIR; PF03460 NIR_SIR_ferr; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.7.1.4 [H]

Molecular weight: Translated: 93519; Mature: 93388

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS00365 NIR_SIR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGPSHEATSRQYTIPSLAPMSTSISNGPARNVVVVGHGMVGHRFVEALRARDDADAWRIT
CCCCCCCCCCEEECCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHCCCCCCCEEEE
VLAEETDAAYDRVGLTGYTEHWDRALLALAGNDYAGDDLVDLRLGQRVTSIDRAARTVKT
EEECCCCHHHHHCCCCCCHHHHHHEEEEEECCCCCCCCEEEHHHCCHHHHHHHHHHHHCC
ADGTAFAYDALVLATGSYAFVPPVPGRDLPACHVYRTFDDLDAIRADAARARETSRAPVG
CCCCCHHHHEEEEECCCEEEECCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCEE
VVIGGGLLGLEAANALRGFGLTAHVVERSPRLMSQQLDDAGGALLSRMIADLGIEVHLDV
EEECCCCHHHHHHHHHHHCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEC
STDAITPAQRNRPLRRSEEDDSLRVMLGDGSFIDAGVVVFAAGVRPRDELARDAGLVIAE
CCCCCCCHHHCCCCCCCCCCCCEEEEECCCCEECCCEEEEECCCCCHHHHHHHCCEEEEE
RGGVLTDLGCKTSDPSIYAVGEVAAIEGRCYGLVGPGYTSAEVVADRLLGGAAEFPEADM
CCCEEEECCCCCCCCCEEEECCEEEECCEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCC
STKLKLLGVDVASFGDAMGRTPDCLEVVVNDAVNQTYAKLVLSDDAKTLLGGVLVGDASA
CCEEEEEECCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHEECCCHHHHHCEEEEECCCH
YGVLRPMVGEQLPGDPMALIAPAGSGGADGGLGVGALPAAAQICSCNNVTKGDVTDAIAG
HHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCHHHHHCC
GCCDVPGLKACTKAGTSCGSCVPLLKQLLEAEGVEQSKALCEHFSHSRAELFEIVSATEI
CCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHH
RTFSGLLERFGTGKGCDICKPTVASILASTSSEHILDGEQAALQDSNDHFLANIQRNGSY
HHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHCCCCCCEEEEEECCCCE
SVVPRVPGGDITPEQLILIGEIARDFDLYTKITGGQRIDLFGARVEQLPEIWRRLVEGGM
EEEECCCCCCCCHHHEEEHHHHHHHHHHHEEECCCCEEEHHHHHHHHHHHHHHHHHHCCC
ESGHAYGKALRTVKSCVGSDWCRYGQQDSVQMAINLELRYRGLRAPHKIKMGVSGCAREC
CCCHHHHHHHHHHHHHHCCHHHHCCCCCCEEEEEEEEEEECCCCCCCEEECCHHHHHHHH
AEARGKDVGVIATEHGWNLYVGGNGGMTPKHAQLLAGDLDDETLIRYVDRFLMFYIRTAD
HHHCCCCEEEEEECCCCEEEECCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
RLQRTAPWVEQLGLDHLREVVCDDSLGLASEFEAAMARHVEGYACEWKGVLEDPDKLSRF
HHHHHHHHHHHHCHHHHHHHHCCCCCCCHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHH
VSFVNAPDVPDPTVTFTENAGRKVPVPIGMPSIRPLQETP
HHHHCCCCCCCCCEEEECCCCCEECCCCCCCCCCCCCCCC
>Mature Secondary Structure 
GPSHEATSRQYTIPSLAPMSTSISNGPARNVVVVGHGMVGHRFVEALRARDDADAWRIT
CCCCCCCCCEEECCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHCCCCCCCEEEE
VLAEETDAAYDRVGLTGYTEHWDRALLALAGNDYAGDDLVDLRLGQRVTSIDRAARTVKT
EEECCCCHHHHHCCCCCCHHHHHHEEEEEECCCCCCCCEEEHHHCCHHHHHHHHHHHHCC
ADGTAFAYDALVLATGSYAFVPPVPGRDLPACHVYRTFDDLDAIRADAARARETSRAPVG
CCCCCHHHHEEEEECCCEEEECCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCEE
VVIGGGLLGLEAANALRGFGLTAHVVERSPRLMSQQLDDAGGALLSRMIADLGIEVHLDV
EEECCCCHHHHHHHHHHHCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEC
STDAITPAQRNRPLRRSEEDDSLRVMLGDGSFIDAGVVVFAAGVRPRDELARDAGLVIAE
CCCCCCCHHHCCCCCCCCCCCCEEEEECCCCEECCCEEEEECCCCCHHHHHHHCCEEEEE
RGGVLTDLGCKTSDPSIYAVGEVAAIEGRCYGLVGPGYTSAEVVADRLLGGAAEFPEADM
CCCEEEECCCCCCCCCEEEECCEEEECCEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCC
STKLKLLGVDVASFGDAMGRTPDCLEVVVNDAVNQTYAKLVLSDDAKTLLGGVLVGDASA
CCEEEEEECCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHEECCCHHHHHCEEEEECCCH
YGVLRPMVGEQLPGDPMALIAPAGSGGADGGLGVGALPAAAQICSCNNVTKGDVTDAIAG
HHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCHHHHHCC
GCCDVPGLKACTKAGTSCGSCVPLLKQLLEAEGVEQSKALCEHFSHSRAELFEIVSATEI
CCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHH
RTFSGLLERFGTGKGCDICKPTVASILASTSSEHILDGEQAALQDSNDHFLANIQRNGSY
HHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHCCCCCCEEEEEECCCCE
SVVPRVPGGDITPEQLILIGEIARDFDLYTKITGGQRIDLFGARVEQLPEIWRRLVEGGM
EEEECCCCCCCCHHHEEEHHHHHHHHHHHEEECCCCEEEHHHHHHHHHHHHHHHHHHCCC
ESGHAYGKALRTVKSCVGSDWCRYGQQDSVQMAINLELRYRGLRAPHKIKMGVSGCAREC
CCCHHHHHHHHHHHHHHCCHHHHCCCCCCEEEEEEEEEEECCCCCCCEEECCHHHHHHHH
AEARGKDVGVIATEHGWNLYVGGNGGMTPKHAQLLAGDLDDETLIRYVDRFLMFYIRTAD
HHHCCCCEEEEEECCCCEEEECCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
RLQRTAPWVEQLGLDHLREVVCDDSLGLASEFEAAMARHVEGYACEWKGVLEDPDKLSRF
HHHHHHHHHHHHCHHHHHHHHCCCCCCCHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHH
VSFVNAPDVPDPTVTFTENAGRKVPVPIGMPSIRPLQETP
HHHHCCCCCCCCCEEEECCCCCEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8468296 [H]