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The map label for this gene is lip2 [H]

Identifier: 108797180

GI number: 108797180

Start: 219896

End: 220801

Strand: Reverse

Name: lip2 [H]

Synonym: Mmcs_0200

Alternate gene names: 108797180

Gene position: 220801-219896 (Counterclockwise)

Preceding gene: 108797181

Following gene: 108797178

Centisome position: 3.87

GC content: 71.41

Gene sequence:

>906_bases
ATGCCGACCCCGCACATCCACGCGTTCCACCCCGACCTGCGCAGCGTCGCCCGCTACATCCCGCGACAGGTGATCACCCC
CGTGACGCTGCCGGTGGTGCGGATGGTGACGAGGCTGCAGGGCAAGCGCACCCCGAAGGACATCGAGGAGCTCACGCTGC
CCTCGGGCGTGCGGGTCCGGCTGTTCCGCCCGACCGGGGTGCCGGTCCCGGCGCCCGCGCTGTTGTGGATCCACGGCGGC
GGCTACGTGATCGGCACGGCCGCACAGGACGACGAACTGTGCCGGCGGTTCTGCCGGAAACTCGGGATCACCGTCGCCTC
GGTGGATTACCGCTTGGCGCCCGAACACCCCTACCCCACCCCGGTCGAGGACTGCTACGAGGCGCTGACGTGGATGGCCC
GGCTGCCGGCGGTGGACCCGAAGCGGGTGGCGATCGGCGGGGCCAGCGCAGGCGGCGGGCTGGCCGCCTCACTGGCCCTG
CTCACCCGCGACCGCGGTGAGATCGAGCCGGTGGCACAGCTTCTGGTGTATCCGATGATCGACGACCGCACCGTCGAGCG
CCACGGCCTGGACCACCCCGGCCACCGGCTGTGGAATCAGGCCAGCAACAAGTTCGGCTGGTCGGCCTACCTCGGTGATG
CCGATCCCGACGTCGCGGTGCCCGCCCGCCGGGAGGATCTGGCGGGCCTGCCGCCTGCGTGGGTGGGTGTCGGCACACTG
GACCTGTTCCACGACGAGGATCTGGCGTACGCCGAGCGGTTGCGGGCCGCCGGCGTGCCGTGCCAGGTCGAGGTGGTCCA
GGGTGCCTTCCACGGATTCGACGGAGTCCTGCCGAAGGCCGACGTGTCGCGGGCGTTCTTCCGAAGCCAGTGCGACACCC
TCCGGCAGGCCTTCGCCTCCGGTTAG

Upstream 100 bases:

>100_bases
CCACTTTCCGCCGAGACTGCGGGGAGATCACGATTCGCGCGCCGCATGCGATCTCCCCGCAGTCTCGGCGCGTCTGCTTG
CGTGCGGTATTACTGGTCAG

Downstream 100 bases:

>100_bases
TCGCGAATCTCGCTGTCGTCGTAGACGATCCGCCCGATCAGGTCGTAGCGGCGGGCGTCACGGTACTTGAAGTACGTGGC
CATGGCCGCCCCGAAGACGA

Product: alpha/beta hydrolase fold-3

Products: NA

Alternate protein names: Triacylglycerol lipase [H]

Number of amino acids: Translated: 301; Mature: 300

Protein sequence:

>301_residues
MPTPHIHAFHPDLRSVARYIPRQVITPVTLPVVRMVTRLQGKRTPKDIEELTLPSGVRVRLFRPTGVPVPAPALLWIHGG
GYVIGTAAQDDELCRRFCRKLGITVASVDYRLAPEHPYPTPVEDCYEALTWMARLPAVDPKRVAIGGASAGGGLAASLAL
LTRDRGEIEPVAQLLVYPMIDDRTVERHGLDHPGHRLWNQASNKFGWSAYLGDADPDVAVPARREDLAGLPPAWVGVGTL
DLFHDEDLAYAERLRAAGVPCQVEVVQGAFHGFDGVLPKADVSRAFFRSQCDTLRQAFASG

Sequences:

>Translated_301_residues
MPTPHIHAFHPDLRSVARYIPRQVITPVTLPVVRMVTRLQGKRTPKDIEELTLPSGVRVRLFRPTGVPVPAPALLWIHGG
GYVIGTAAQDDELCRRFCRKLGITVASVDYRLAPEHPYPTPVEDCYEALTWMARLPAVDPKRVAIGGASAGGGLAASLAL
LTRDRGEIEPVAQLLVYPMIDDRTVERHGLDHPGHRLWNQASNKFGWSAYLGDADPDVAVPARREDLAGLPPAWVGVGTL
DLFHDEDLAYAERLRAAGVPCQVEVVQGAFHGFDGVLPKADVSRAFFRSQCDTLRQAFASG
>Mature_300_residues
PTPHIHAFHPDLRSVARYIPRQVITPVTLPVVRMVTRLQGKRTPKDIEELTLPSGVRVRLFRPTGVPVPAPALLWIHGGG
YVIGTAAQDDELCRRFCRKLGITVASVDYRLAPEHPYPTPVEDCYEALTWMARLPAVDPKRVAIGGASAGGGLAASLALL
TRDRGEIEPVAQLLVYPMIDDRTVERHGLDHPGHRLWNQASNKFGWSAYLGDADPDVAVPARREDLAGLPPAWVGVGTLD
LFHDEDLAYAERLRAAGVPCQVEVVQGAFHGFDGVLPKADVSRAFFRSQCDTLRQAFASG

Specific function: Has An Esterase Activity. Triacetyl Glycerol (Triacetin) Is A Substrate Of The Enzyme. [C]

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI68299767, Length=268, Percent_Identity=29.4776119402985, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI157041239, Length=113, Percent_Identity=36.283185840708, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI206597554, Length=267, Percent_Identity=27.3408239700375, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI68051721, Length=138, Percent_Identity=34.0579710144928, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI21328446, Length=153, Percent_Identity=29.4117647058824, Blast_Score=69, Evalue=5e-12,
Organism=Escherichia coli, GI1786682, Length=229, Percent_Identity=28.82096069869, Blast_Score=70, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI72001146, Length=133, Percent_Identity=37.593984962406, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17567059, Length=182, Percent_Identity=32.4175824175824, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17540028, Length=128, Percent_Identity=33.59375, Blast_Score=75, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI71996133, Length=133, Percent_Identity=30.0751879699248, Blast_Score=72, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: =3.1.1.3 [H]

Molecular weight: Translated: 32912; Mature: 32781

Theoretical pI: Translated: 7.31; Mature: 7.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTPHIHAFHPDLRSVARYIPRQVITPVTLPVVRMVTRLQGKRTPKDIEELTLPSGVRVR
CCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCEEE
LFRPTGVPVPAPALLWIHGGGYVIGTAAQDDELCRRFCRKLGITVASVDYRLAPEHPYPT
EECCCCCCCCCCEEEEEECCCEEEECCCCCHHHHHHHHHHHCCEEEEECEEECCCCCCCC
PVEDCYEALTWMARLPAVDPKRVAIGGASAGGGLAASLALLTRDRGEIEPVAQLLVYPMI
CHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCC
DDRTVERHGLDHPGHRLWNQASNKFGWSAYLGDADPDVAVPARREDLAGLPPAWVGVGTL
CCCCHHHCCCCCCHHHHHHHHCCCCCCEEECCCCCCCCCCCCCHHHCCCCCCHHHCCCCC
DLFHDEDLAYAERLRAAGVPCQVEVVQGAFHGFDGVLPKADVSRAFFRSQCDTLRQAFAS
EECCCCHHHHHHHHHHCCCCEEEHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHC
G
C
>Mature Secondary Structure 
PTPHIHAFHPDLRSVARYIPRQVITPVTLPVVRMVTRLQGKRTPKDIEELTLPSGVRVR
CCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCEEE
LFRPTGVPVPAPALLWIHGGGYVIGTAAQDDELCRRFCRKLGITVASVDYRLAPEHPYPT
EECCCCCCCCCCEEEEEECCCEEEECCCCCHHHHHHHHHHHCCEEEEECEEECCCCCCCC
PVEDCYEALTWMARLPAVDPKRVAIGGASAGGGLAASLALLTRDRGEIEPVAQLLVYPMI
CHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCC
DDRTVERHGLDHPGHRLWNQASNKFGWSAYLGDADPDVAVPARREDLAGLPPAWVGVGTL
CCCCHHHCCCCCCHHHHHHHHCCCCCCEEECCCCCCCCCCCCCHHHCCCCCCHHHCCCCC
DLFHDEDLAYAERLRAAGVPCQVEVVQGAFHGFDGVLPKADVSRAFFRSQCDTLRQAFAS
EECCCCHHHHHHHHHHCCCCEEEHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHC
G
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1907455 [H]