Click here to switch to the map view.

The map label for this gene is murD [H]

Identifier: 108562895

GI number: 108562895

Start: 486309

End: 487577

Strand: Direct

Name: murD [H]

Synonym: HPAG1_0470

Alternate gene names: 108562895

Gene position: 486309-487577 (Clockwise)

Preceding gene: 108562894

Following gene: 108562898

Centisome position: 30.46

GC content: 36.88

Gene sequence:

>1269_bases
ATGAAAATCTCTTTATTGGGGCATGGCAAAACCACTCTAGCCCTAGCGCGTTTTTTTAAAAAAAACCATAACGAAGTCAA
ATTTTTTGATGATCAATTCACTTCATTCCATAAGGATAGAGAGGGTTTTCTTTGCCACCCTAGTAAGGATTTCAACCCTA
ACGATTCCCAATTAGAGGTAGTCAGCCCTGGCATTAGTTTCACGCACCCTTTAGTCATAAAAGCCAAGCATTTAGTGAGC
GAATACGATTATATTGACAGCTTGTTTGATTTGGTTTTCACGCCTACTATAATCAGTATTAGCGGCACTAACGGGAAAAC
CACCACGACCGAAATGCTCACCATGCTTTTAGAAGATTTTAAGGCTGTGAGTGGGGGGAATATCGGCACGCCCTTGATTG
AATTGTTTGAAAAACAATCGCCCTTGTGGGTGCTAGAAACAAGCTCTTTTTCTTTGCATTACACTAATAAGGCTTACCCT
TTAATCTACTTGCTCATCAATGTAGAAGCCGATCATTTGACTTGGCATTGCAATTTTGAAAATTATTTGAACGCTAAACT
CAAGGTTCTAACATTGATGCCTAAAACTTCGCTCGCTATCATCCCTTTAAAATTCAAAGAACACCCCATTATTCAAAACT
CGCAAGCGCAAAAAATCTTTTTTGACAAAAGCGAAGAGATTTTAGAGTGTTTAAAAATCCCTTCTAACGCCCTTTTTTTT
AAGGGAGCGTTTTTATTAGACGCGGCTTTAGCCCTTTTAGTTTATGAGCAATTTTTAAAAATAAAAAATTTAAAATGGCA
AGATTATAGAGAAAACGCCCTTAAAAGACTGAACGCTTTTAAAATTGGCTCGCATAAAATGGAAGAATTTAGGGATAAAC
AAGGGCGTTTGTGGGTAGATGACAGCAAAGCCACGAATATTGATGCCACCTTACAAGCCCTAAAAACCTTTAAAAACCAA
AAAATCCATTTGATTGTAGGGGGCGATATTAAAGGGGTCAATTTAACCCCCCTTTTTGAAGAATTTAAAAATCATGAAGT
AAGCCTTTATGCGATAGGATCAAGCGCTTCTATCATCCAAGCCTTAGCGTTAGAATTTAATGTTTCTTGTCAGGTTTGTT
TAAAGTTAGAAAAAGCGGTTCAAGAAATTAAAAGCGTTTTATCGCAAAATGAAATCGCTTTGCTTTCACCTAGTGCAGCC
AGTTTGGATCAATTTTCTTCGTATAAAGAAAGGGGTGAAAAATTTAAAGCGTTTGTTTTAAAGGATTAA

Upstream 100 bases:

>100_bases
GAGCAAAAGGGTTGGGCAGAAAACAAAGTGATCGTGCGTTTTTGGATCATTTCTATGCTGAGTAATTTAGTCGCTCTTTT
AAGCTTGAAGGTGCGTTAAA

Downstream 100 bases:

>100_bases
AGCGTTTGAACCACTTTGTCTAATTGTGAAATTTTTTGAAAAATCTCGTTCCGTTCTGATTCGTTTGAAACTTCCATAGA
AACATTGAAGCTATAAAACT

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase [H]

Number of amino acids: Translated: 422; Mature: 422

Protein sequence:

>422_residues
MKISLLGHGKTTLALARFFKKNHNEVKFFDDQFTSFHKDREGFLCHPSKDFNPNDSQLEVVSPGISFTHPLVIKAKHLVS
EYDYIDSLFDLVFTPTIISISGTNGKTTTTEMLTMLLEDFKAVSGGNIGTPLIELFEKQSPLWVLETSSFSLHYTNKAYP
LIYLLINVEADHLTWHCNFENYLNAKLKVLTLMPKTSLAIIPLKFKEHPIIQNSQAQKIFFDKSEEILECLKIPSNALFF
KGAFLLDAALALLVYEQFLKIKNLKWQDYRENALKRLNAFKIGSHKMEEFRDKQGRLWVDDSKATNIDATLQALKTFKNQ
KIHLIVGGDIKGVNLTPLFEEFKNHEVSLYAIGSSASIIQALALEFNVSCQVCLKLEKAVQEIKSVLSQNEIALLSPSAA
SLDQFSSYKERGEKFKAFVLKD

Sequences:

>Translated_422_residues
MKISLLGHGKTTLALARFFKKNHNEVKFFDDQFTSFHKDREGFLCHPSKDFNPNDSQLEVVSPGISFTHPLVIKAKHLVS
EYDYIDSLFDLVFTPTIISISGTNGKTTTTEMLTMLLEDFKAVSGGNIGTPLIELFEKQSPLWVLETSSFSLHYTNKAYP
LIYLLINVEADHLTWHCNFENYLNAKLKVLTLMPKTSLAIIPLKFKEHPIIQNSQAQKIFFDKSEEILECLKIPSNALFF
KGAFLLDAALALLVYEQFLKIKNLKWQDYRENALKRLNAFKIGSHKMEEFRDKQGRLWVDDSKATNIDATLQALKTFKNQ
KIHLIVGGDIKGVNLTPLFEEFKNHEVSLYAIGSSASIIQALALEFNVSCQVCLKLEKAVQEIKSVLSQNEIALLSPSAA
SLDQFSSYKERGEKFKAFVLKD
>Mature_422_residues
MKISLLGHGKTTLALARFFKKNHNEVKFFDDQFTSFHKDREGFLCHPSKDFNPNDSQLEVVSPGISFTHPLVIKAKHLVS
EYDYIDSLFDLVFTPTIISISGTNGKTTTTEMLTMLLEDFKAVSGGNIGTPLIELFEKQSPLWVLETSSFSLHYTNKAYP
LIYLLINVEADHLTWHCNFENYLNAKLKVLTLMPKTSLAIIPLKFKEHPIIQNSQAQKIFFDKSEEILECLKIPSNALFF
KGAFLLDAALALLVYEQFLKIKNLKWQDYRENALKRLNAFKIGSHKMEEFRDKQGRLWVDDSKATNIDATLQALKTFKNQ
KIHLIVGGDIKGVNLTPLFEEFKNHEVSLYAIGSSASIIQALALEFNVSCQVCLKLEKAVQEIKSVLSQNEIALLSPSAA
SLDQFSSYKERGEKFKAFVLKD

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) [H]

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786276, Length=451, Percent_Identity=25.9423503325942, Blast_Score=130, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR005762 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.9 [H]

Molecular weight: Translated: 47885; Mature: 47885

Theoretical pI: Translated: 7.96; Mature: 7.96

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKISLLGHGKTTLALARFFKKNHNEVKFFDDQFTSFHKDREGFLCHPSKDFNPNDSQLEV
CEEEEECCCHHHHHHHHHHHCCCCCEEEECHHHHHHHCCCCCEEECCCCCCCCCCCEEEE
VSPGISFTHPLVIKAKHLVSEYDYIDSLFDLVFTPTIISISGTNGKTTTTEMLTMLLEDF
ECCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHH
KAVSGGNIGTPLIELFEKQSPLWVLETSSFSLHYTNKAYPLIYLLINVEADHLTWHCNFE
HHCCCCCCCCHHHHHHHCCCCEEEEECCCEEEEECCCCCEEEEEEEEEEECEEEEEECHH
NYLNAKLKVLTLMPKTSLAIIPLKFKEHPIIQNSQAQKIFFDKSEEILECLKIPSNALFF
HHCCCEEEEEEEECCCCEEEEEEECCCCCCCCCCCCCEEEECCHHHHHHHHHCCCCCEEE
KGAFLLDAALALLVYEQFLKIKNLKWQDYRENALKRLNAFKIGSHKMEEFRDKQGRLWVD
CHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCEEEE
DSKATNIDATLQALKTFKNQKIHLIVGGDIKGVNLTPLFEEFKNHEVSLYAIGSSASIIQ
CCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHCCCCEEEEEECCCHHHHH
ALALEFNVSCQVCLKLEKAVQEIKSVLSQNEIALLSPSAASLDQFSSYKERGEKFKAFVL
HHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHCCCEEEEEE
KD
CC
>Mature Secondary Structure
MKISLLGHGKTTLALARFFKKNHNEVKFFDDQFTSFHKDREGFLCHPSKDFNPNDSQLEV
CEEEEECCCHHHHHHHHHHHCCCCCEEEECHHHHHHHCCCCCEEECCCCCCCCCCCEEEE
VSPGISFTHPLVIKAKHLVSEYDYIDSLFDLVFTPTIISISGTNGKTTTTEMLTMLLEDF
ECCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHH
KAVSGGNIGTPLIELFEKQSPLWVLETSSFSLHYTNKAYPLIYLLINVEADHLTWHCNFE
HHCCCCCCCCHHHHHHHCCCCEEEEECCCEEEEECCCCCEEEEEEEEEEECEEEEEECHH
NYLNAKLKVLTLMPKTSLAIIPLKFKEHPIIQNSQAQKIFFDKSEEILECLKIPSNALFF
HHCCCEEEEEEEECCCCEEEEEEECCCCCCCCCCCCCEEEECCHHHHHHHHHCCCCCEEE
KGAFLLDAALALLVYEQFLKIKNLKWQDYRENALKRLNAFKIGSHKMEEFRDKQGRLWVD
CHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCEEEE
DSKATNIDATLQALKTFKNQKIHLIVGGDIKGVNLTPLFEEFKNHEVSLYAIGSSASIIQ
CCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHCCCCEEEEEECCCHHHHH
ALALEFNVSCQVCLKLEKAVQEIKSVLSQNEIALLSPSAASLDQFSSYKERGEKFKAFVL
HHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHCCCEEEEEE
KD
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA