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The map label for this gene is lcfA [H]

Identifier: 103485982

GI number: 103485982

Start: 505224

End: 506933

Strand: Direct

Name: lcfA [H]

Synonym: Sala_0489

Alternate gene names: 103485982

Gene position: 505224-506933 (Clockwise)

Preceding gene: 103485975

Following gene: 103485983

Centisome position: 15.1

GC content: 63.68

Gene sequence:

>1710_bases
ATGCCATCAGCGCTCGATTTGCGCCTGGAAGCCGCCTATAATCTGATCACCGGTCCCGGCGGACCGATCCAGGTCGGGTC
GGTCGAACGCTTTGGCCGCCAGCTGCCCTTCATCACCAACGCGCCAACCAACCTCGCCGACTATGTCGCCTATTTCGCGA
TGCAGCACGGCGACGCGACCTTTCTGGTCGAGGGCGACGAACGCCTGTCGTTCAAACAGGTCTATATGGCGGCGCGGCAG
GTCGCGGCGGGGCTGATCGAGGGTTTCGGCGTCGAGCGCGGCGACCGGGTGGGTCTGGCGATGCGCAACGCGAATGCGTG
GTGCGTCGCCTATATCGGCATTTTGATGGCGGGCGGCTGCGCGACGCTGCTGAATGGCTGGTGGCAGGGCGGCGAGCTCG
CCGCGGGCATCGAGAACAGCGAGGCGAAGCTGGTCATTGCCGATCCGCAGCGCGCGGCGCGGCTGGCCGAACCGGGGGTT
GAGCATGGCGCGAAGGTCGTGACGCTCGACATCACCCGGCCCATCGACGAAGCGATCGCGCCGATTGCGTCAAAGGGAAG
CGCCGCAACGGTGCTGCCGACGCTCACCGGACAGGATCTCGCGACGATCCTTTTCACCTCGGGCTCGACCGGCCAGTCGA
AGGGGGCCTATTCGCGCCACGAGGCGGTGGTGCAGGCGATCTTCAACTATGTCACGCAGACCGCGACCATCGTCCATCTG
CTGACCGAGGACGGGCAGATGTCCGACATCCAGCCCGCGACGCTGATCTGCACCCCACTGTTTCACGTCACCGCCGAAAT
CCCGGTGTTCCTGCAAAGCTTCGCGCTCGGGCGAAAACTGGTGCTGATGCCCAAATGGAACGCCGAAGAGGCGATGCGAC
TGATCCAGGACGAACAGGTCAATTATTTCGTCGGCGTCCCGCTGATGAGCTATGAGATCCTCACCCATCCGAACCGCAAG
AATTACGACCTGTCGACGTGCAAAAGCTATGCCGGCGGCGGCGCGCCGCGTCCGCCCGAGCATGTGAAGCGTCTCGCCAC
CGAAATGGGCGAGGCGAAGCCCTTGCTCGGCTATGGCCTCACCGAAACCAACGCCGTCGGCTGCGGCATCATCAACGAAA
ATTACGTCGCCAAGCCGCTGTCGACCGGCCCCGCGTCGAAGCCGCTCGTCGATCTGGCGATCCTCGACGACAATGGCGAT
CCGGTGCCGCAGGGCGGCATCGGCGAAGTCTGCATCCGCTCGGTCGCCAATTTCGAAGGCTATTGGAACAATGAGGCGGC
GACCAGGGCGGCCTTTTTCGACAATGGCTATTTCCGCACGGGCGACCTCGGCTATCTCGACGAGGACGGATATCTCTTCA
TTGTCGACCGCAAGAAGGACATCATCATCCGCGGCGGCGAGAATATCAGCTGCCAGGAAGTCGAGGCGGCGATCTATGAG
CATCCCGAAACGAACGAATGCGCGGTGTTCGGGCTGCCCGACGAGCGGCTCGGCGAAGTCGTCGGAGCGGTGATCTGGAT
GAAGCCCGGAAGCGGCGTGACCGCCGACGCCATGTGCTCCTTCCTCAGCGCGCGGCTCGCGCCCTACAAGGTGCCGTACA
AGATCTGGATGTCGAACGAGGCGCTGCCCAAGCTCGGCAGCGAAAAGATCGACAAGGTCAGCCTGCGCAGCCAGTATCGC
GAAGCCTATGCGGCGGAGGTCGTCGCGTAA

Upstream 100 bases:

>100_bases
GCTAAGGTTGCAAAAAGAAACATATAGTCGCGCGGTCCTTCCCCAGGAGAGGCCTCCCCATAAGGGGTAAGGCGAGCGAG
ACCAAGAGGAGAGATGCACG

Downstream 100 bases:

>100_bases
CCAAATGGTGCCGGGAACCGAACCCTTTTCAAGCCCGTTCCCTGAAGGATGAGGGGCTGGGAGGGTGTGCTGGAAACGAT
GACCGATCTTGCTCCCGCCC

Product: AMP-dependent synthetase and ligase

Products: NA

Alternate protein names: Long-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 569; Mature: 568

Protein sequence:

>569_residues
MPSALDLRLEAAYNLITGPGGPIQVGSVERFGRQLPFITNAPTNLADYVAYFAMQHGDATFLVEGDERLSFKQVYMAARQ
VAAGLIEGFGVERGDRVGLAMRNANAWCVAYIGILMAGGCATLLNGWWQGGELAAGIENSEAKLVIADPQRAARLAEPGV
EHGAKVVTLDITRPIDEAIAPIASKGSAATVLPTLTGQDLATILFTSGSTGQSKGAYSRHEAVVQAIFNYVTQTATIVHL
LTEDGQMSDIQPATLICTPLFHVTAEIPVFLQSFALGRKLVLMPKWNAEEAMRLIQDEQVNYFVGVPLMSYEILTHPNRK
NYDLSTCKSYAGGGAPRPPEHVKRLATEMGEAKPLLGYGLTETNAVGCGIINENYVAKPLSTGPASKPLVDLAILDDNGD
PVPQGGIGEVCIRSVANFEGYWNNEAATRAAFFDNGYFRTGDLGYLDEDGYLFIVDRKKDIIIRGGENISCQEVEAAIYE
HPETNECAVFGLPDERLGEVVGAVIWMKPGSGVTADAMCSFLSARLAPYKVPYKIWMSNEALPKLGSEKIDKVSLRSQYR
EAYAAEVVA

Sequences:

>Translated_569_residues
MPSALDLRLEAAYNLITGPGGPIQVGSVERFGRQLPFITNAPTNLADYVAYFAMQHGDATFLVEGDERLSFKQVYMAARQ
VAAGLIEGFGVERGDRVGLAMRNANAWCVAYIGILMAGGCATLLNGWWQGGELAAGIENSEAKLVIADPQRAARLAEPGV
EHGAKVVTLDITRPIDEAIAPIASKGSAATVLPTLTGQDLATILFTSGSTGQSKGAYSRHEAVVQAIFNYVTQTATIVHL
LTEDGQMSDIQPATLICTPLFHVTAEIPVFLQSFALGRKLVLMPKWNAEEAMRLIQDEQVNYFVGVPLMSYEILTHPNRK
NYDLSTCKSYAGGGAPRPPEHVKRLATEMGEAKPLLGYGLTETNAVGCGIINENYVAKPLSTGPASKPLVDLAILDDNGD
PVPQGGIGEVCIRSVANFEGYWNNEAATRAAFFDNGYFRTGDLGYLDEDGYLFIVDRKKDIIIRGGENISCQEVEAAIYE
HPETNECAVFGLPDERLGEVVGAVIWMKPGSGVTADAMCSFLSARLAPYKVPYKIWMSNEALPKLGSEKIDKVSLRSQYR
EAYAAEVVA
>Mature_568_residues
PSALDLRLEAAYNLITGPGGPIQVGSVERFGRQLPFITNAPTNLADYVAYFAMQHGDATFLVEGDERLSFKQVYMAARQV
AAGLIEGFGVERGDRVGLAMRNANAWCVAYIGILMAGGCATLLNGWWQGGELAAGIENSEAKLVIADPQRAARLAEPGVE
HGAKVVTLDITRPIDEAIAPIASKGSAATVLPTLTGQDLATILFTSGSTGQSKGAYSRHEAVVQAIFNYVTQTATIVHLL
TEDGQMSDIQPATLICTPLFHVTAEIPVFLQSFALGRKLVLMPKWNAEEAMRLIQDEQVNYFVGVPLMSYEILTHPNRKN
YDLSTCKSYAGGGAPRPPEHVKRLATEMGEAKPLLGYGLTETNAVGCGIINENYVAKPLSTGPASKPLVDLAILDDNGDP
VPQGGIGEVCIRSVANFEGYWNNEAATRAAFFDNGYFRTGDLGYLDEDGYLFIVDRKKDIIIRGGENISCQEVEAAIYEH
PETNECAVFGLPDERLGEVVGAVIWMKPGSGVTADAMCSFLSARLAPYKVPYKIWMSNEALPKLGSEKIDKVSLRSQYRE
AYAAEVVA

Specific function: Unknown

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=534, Percent_Identity=27.3408239700375, Blast_Score=193, Evalue=4e-49,
Organism=Homo sapiens, GI187761345, Length=585, Percent_Identity=25.982905982906, Blast_Score=135, Evalue=1e-31,
Organism=Homo sapiens, GI187761343, Length=585, Percent_Identity=25.982905982906, Blast_Score=135, Evalue=1e-31,
Organism=Homo sapiens, GI42544132, Length=515, Percent_Identity=26.2135922330097, Blast_Score=126, Evalue=4e-29,
Organism=Homo sapiens, GI122937307, Length=528, Percent_Identity=25.9469696969697, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI115511026, Length=527, Percent_Identity=25.6166982922201, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI38505220, Length=512, Percent_Identity=25.9765625, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI58082049, Length=509, Percent_Identity=24.3614931237721, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI157311624, Length=510, Percent_Identity=24.1176470588235, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI157311622, Length=510, Percent_Identity=24.1176470588235, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI28416953, Length=531, Percent_Identity=25.6120527306968, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI227499619, Length=540, Percent_Identity=23.7037037037037, Blast_Score=82, Evalue=2e-15,
Organism=Homo sapiens, GI13375727, Length=120, Percent_Identity=34.1666666666667, Blast_Score=77, Evalue=5e-14,
Organism=Homo sapiens, GI42794754, Length=250, Percent_Identity=31.2, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI42794752, Length=250, Percent_Identity=31.2, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI27477105, Length=123, Percent_Identity=35.7723577235772, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI145693145, Length=526, Percent_Identity=27.9467680608365, Blast_Score=170, Evalue=2e-43,
Organism=Escherichia coli, GI1788107, Length=552, Percent_Identity=28.2608695652174, Blast_Score=165, Evalue=7e-42,
Organism=Escherichia coli, GI221142682, Length=490, Percent_Identity=26.3265306122449, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI1786810, Length=518, Percent_Identity=25.4826254826255, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1790505, Length=549, Percent_Identity=24.2258652094718, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1788595, Length=377, Percent_Identity=26.525198938992, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1789201, Length=501, Percent_Identity=25.3493013972056, Blast_Score=71, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI71994694, Length=538, Percent_Identity=26.5799256505576, Blast_Score=175, Evalue=6e-44,
Organism=Caenorhabditis elegans, GI71994703, Length=538, Percent_Identity=26.5799256505576, Blast_Score=175, Evalue=6e-44,
Organism=Caenorhabditis elegans, GI71994690, Length=538, Percent_Identity=26.5799256505576, Blast_Score=175, Evalue=7e-44,
Organism=Caenorhabditis elegans, GI17558820, Length=513, Percent_Identity=27.0955165692008, Blast_Score=160, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI17560308, Length=533, Percent_Identity=26.078799249531, Blast_Score=151, Evalue=8e-37,
Organism=Caenorhabditis elegans, GI32563687, Length=510, Percent_Identity=25.0980392156863, Blast_Score=147, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17531443, Length=523, Percent_Identity=23.3269598470363, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17559526, Length=525, Percent_Identity=24.952380952381, Blast_Score=132, Evalue=7e-31,
Organism=Caenorhabditis elegans, GI17557194, Length=536, Percent_Identity=22.7611940298507, Blast_Score=112, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI17560140, Length=533, Percent_Identity=23.6397748592871, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17538037, Length=543, Percent_Identity=22.0994475138122, Blast_Score=100, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI71985884, Length=379, Percent_Identity=23.4828496042216, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI25147511, Length=172, Percent_Identity=36.6279069767442, Blast_Score=71, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI25141359, Length=457, Percent_Identity=23.1947483588621, Blast_Score=69, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6319699, Length=550, Percent_Identity=26.7272727272727, Blast_Score=164, Evalue=3e-41,
Organism=Saccharomyces cerevisiae, GI6319264, Length=562, Percent_Identity=22.9537366548043, Blast_Score=88, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6323182, Length=551, Percent_Identity=22.3230490018149, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24581924, Length=541, Percent_Identity=27.1719038817006, Blast_Score=162, Evalue=4e-40,
Organism=Drosophila melanogaster, GI18859661, Length=535, Percent_Identity=24.1121495327103, Blast_Score=157, Evalue=1e-38,
Organism=Drosophila melanogaster, GI21355181, Length=537, Percent_Identity=24.7672253258845, Blast_Score=132, Evalue=6e-31,
Organism=Drosophila melanogaster, GI24648255, Length=454, Percent_Identity=25.5506607929515, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24648253, Length=454, Percent_Identity=25.1101321585903, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24648260, Length=482, Percent_Identity=25.3112033195021, Blast_Score=122, Evalue=5e-28,
Organism=Drosophila melanogaster, GI21356441, Length=458, Percent_Identity=23.1441048034935, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24653035, Length=465, Percent_Identity=23.8709677419355, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24656500, Length=520, Percent_Identity=23.8461538461538, Blast_Score=113, Evalue=4e-25,
Organism=Drosophila melanogaster, GI19922652, Length=495, Percent_Identity=24.8484848484848, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI281365686, Length=248, Percent_Identity=27.8225806451613, Blast_Score=109, Evalue=6e-24,
Organism=Drosophila melanogaster, GI21358303, Length=206, Percent_Identity=31.5533980582524, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI21356947, Length=468, Percent_Identity=23.7179487179487, Blast_Score=106, Evalue=5e-23,
Organism=Drosophila melanogaster, GI161076582, Length=472, Percent_Identity=23.3050847457627, Blast_Score=104, Evalue=2e-22,
Organism=Drosophila melanogaster, GI20130357, Length=429, Percent_Identity=23.3100233100233, Blast_Score=96, Evalue=8e-20,
Organism=Drosophila melanogaster, GI21355225, Length=492, Percent_Identity=24.7967479674797, Blast_Score=89, Evalue=8e-18,
Organism=Drosophila melanogaster, GI24648257, Length=467, Percent_Identity=23.3404710920771, Blast_Score=86, Evalue=7e-17,
Organism=Drosophila melanogaster, GI281364798, Length=561, Percent_Identity=23.3511586452763, Blast_Score=80, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24583465, Length=561, Percent_Identity=23.3511586452763, Blast_Score=79, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24583463, Length=561, Percent_Identity=23.3511586452763, Blast_Score=79, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.3 [H]

Molecular weight: Translated: 61398; Mature: 61266

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS00455 AMP_BINDING ; PS00287 CYSTATIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSALDLRLEAAYNLITGPGGPIQVGSVERFGRQLPFITNAPTNLADYVAYFAMQHGDAT
CCCCCEEEEEEHEEEEECCCCCEEECCHHHHCCCCCEEECCCCHHHHHHHHHHHCCCCEE
FLVEGDERLSFKQVYMAARQVAAGLIEGFGVERGDRVGLAMRNANAWCVAYIGILMAGGC
EEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHHHCCH
ATLLNGWWQGGELAAGIENSEAKLVIADPQRAARLAEPGVEHGAKVVTLDITRPIDEAIA
HHHHHCCCCCCCEEECCCCCCCEEEEECCHHHHHHHCCCHHCCCEEEEEEECCCHHHHHH
PIASKGSAATVLPTLTGQDLATILFTSGSTGQSKGAYSRHEAVVQAIFNYVTQTATIVHL
HHCCCCCCEEEEECCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEE
LTEDGQMSDIQPATLICTPLFHVTAEIPVFLQSFALGRKLVLMPKWNAEEAMRLIQDEQV
EECCCCCCCCCCHHEEEHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHCCC
NYFVGVPLMSYEILTHPNRKNYDLSTCKSYAGGGAPRPPEHVKRLATEMGEAKPLLGYGL
CEEECCCEEEEEEEECCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCEECCC
TETNAVGCGIINENYVAKPLSTGPASKPLVDLAILDDNGDPVPQGGIGEVCIRSVANFEG
CCCCCEEEEEECCCCEECCCCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHCCC
YWNNEAATRAAFFDNGYFRTGDLGYLDEDGYLFIVDRKKDIIIRGGENISCQEVEAAIYE
EECCCHHHEEEEECCCCEECCCCCEECCCCEEEEEECCCEEEEECCCCCCHHHHHHHHHC
HPETNECAVFGLPDERLGEVVGAVIWMKPGSGVTADAMCSFLSARLAPYKVPYKIWMSNE
CCCCCCEEEEECCHHHHHHHHEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCEEEEECCC
ALPKLGSEKIDKVSLRSQYREAYAAEVVA
CCCCCCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PSALDLRLEAAYNLITGPGGPIQVGSVERFGRQLPFITNAPTNLADYVAYFAMQHGDAT
CCCCEEEEEEHEEEEECCCCCEEECCHHHHCCCCCEEECCCCHHHHHHHHHHHCCCCEE
FLVEGDERLSFKQVYMAARQVAAGLIEGFGVERGDRVGLAMRNANAWCVAYIGILMAGGC
EEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHHHCCH
ATLLNGWWQGGELAAGIENSEAKLVIADPQRAARLAEPGVEHGAKVVTLDITRPIDEAIA
HHHHHCCCCCCCEEECCCCCCCEEEEECCHHHHHHHCCCHHCCCEEEEEEECCCHHHHHH
PIASKGSAATVLPTLTGQDLATILFTSGSTGQSKGAYSRHEAVVQAIFNYVTQTATIVHL
HHCCCCCCEEEEECCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEE
LTEDGQMSDIQPATLICTPLFHVTAEIPVFLQSFALGRKLVLMPKWNAEEAMRLIQDEQV
EECCCCCCCCCCHHEEEHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHCCC
NYFVGVPLMSYEILTHPNRKNYDLSTCKSYAGGGAPRPPEHVKRLATEMGEAKPLLGYGL
CEEECCCEEEEEEEECCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCEECCC
TETNAVGCGIINENYVAKPLSTGPASKPLVDLAILDDNGDPVPQGGIGEVCIRSVANFEG
CCCCCEEEEEECCCCEECCCCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHCCC
YWNNEAATRAAFFDNGYFRTGDLGYLDEDGYLFIVDRKKDIIIRGGENISCQEVEAAIYE
EECCCHHHEEEEECCCCEECCCCCEECCCCEEEEEECCCEEEEECCCCCCHHHHHHHHHC
HPETNECAVFGLPDERLGEVVGAVIWMKPGSGVTADAMCSFLSARLAPYKVPYKIWMSNE
CCCCCCEEEEECCHHHHHHHHEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCEEEEECCC
ALPKLGSEKIDKVSLRSQYREAYAAEVVA
CCCCCCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]