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The map label for this gene is cls [H]

Identifier: 103485881

GI number: 103485881

Start: 398363

End: 399766

Strand: Direct

Name: cls [H]

Synonym: Sala_0386

Alternate gene names: 103485881

Gene position: 398363-399766 (Clockwise)

Preceding gene: 103485880

Following gene: 103485882

Centisome position: 11.91

GC content: 57.69

Gene sequence:

>1404_bases
ATGATATTTTCAATCGCGGTGATCGCTGCGATTGCCACGGCCAGCCACGCGATCATCCACAAGCGCGACCCGCGATCGGC
GACCATCTGGCTGCTAGTGGTTGCTCTTGTCCCTCTAGGCGGCTCACTTTGCTACCTTTTATTTGGGATAAATCGGGTTA
ACCGCAAAGCAATGCGGCTACGCGCGGCGCGCCCAGCGAAAAGCGAACTTTCCAATCTAGGTTCGGCGGGCATGGATTTG
CCGCCTCATCTGGCCAGTCTTGCGCGTCTCGGGCGAGACGTGTCAGGCAAAGAACTGTTGCCCGGAAATCGCTGCACCCC
CCTTTTTGGGGGCACAGAGGCCTACCCGCAAATGCTTCAGGCGATTTGCGAAGCGAAGCATTCTATCGCCCTTGCCTCGT
ACATTTTCGAGGGCAAGGGGATCGGGGCGCTTTTCGTCGATACCCTCGCGCAAGCGCGGTCGCGCGGAGTGAGCGTCAAG
GTCCTGATCGATGATGCCTATGCCAGGTTATCCAACTCGGACGCCTACGGAGCATTGCGGCTTCACGGTATTGATGTCGC
CCTGTTCAATCCCCCTGTGATACCGGCACGCCTGCACGGGTTGCATCTGCGCAACCATCGCAAACTGCTCATCGTTGACG
GGTACCGGGGCTTTACCGGCGGCATGAACATCCATAGGCCCTATTGGTGTCCCAAGGCCCCGGAAAGCGCTTTCCGCGAT
CTGCACCTTGAGGTTTCGGGTCCGGTCGTCGGGCAAATGCGGGAAACCTTCGAGCGGGACTGGTACGATAGCAAGGAAGA
AGAGCTGGACGAAAGCTTCTGGACTGGCAAATCGGGCGGTCCGATCACCGACGGATTTAGCCTTGCGCGCGGGATCGAAT
CCGGACCTGACGAAACGCTGGACCGGATGCGGTGGATTTTCATAGGCGCAATCAATGCCGCTCAGAAAAAGATTTCCATC
CGCACACCTTATTTCCTGCCTGACCAGCCTACTATTGCCGCGCTGGGCGCAGCCGCTTTGCGAGGGGTTCGGGTCGAGAT
CGTGGTCCCGCGAAACAGCGACCATCTGTTCGTGCAATGGGCATCTAAGGCGCATTACTGGCAGATAATCGAACACGGTG
TCTGCATTTATGAGCGCCCCGGCCCGTTCGATCATGGCAAGCTGATGATAATTGACGATGCCTGGGTGTGTGTCGGCTCC
GCCAACTGGGATGCGCGTAGCCTGCGCCTGAACTTCGAATTCAACCTGGAAATTTACGATCGCAGGCTGGCGCTGGAGCT
TGGTGACGATTTCGCCCGAATCAGGTCTGCGTCGAAGAAAGTGAGGCCGACCGACCTGTCTGACATGCCGTATTTCGTGA
AACTGCGAAATGGCTTTGCGCGGCTGTTTTCGCCGATCCTTTGA

Upstream 100 bases:

>100_bases
CGTGGGAAGAAATTTTCCGAAGCAAAGGTTGCCGCTGCTGCCGTCAGGCACCGATGCGTACCGGGGTATGAACATTCCTG
AAACTACGTCGTTTAGCTGG

Downstream 100 bases:

>100_bases
AACTGGCGGAAAGCGATAATGGAAGAACTCTTTTTCTCTGTGTTCATTCTCTTTGCTTCCATCCTGGTGGCCGGCTTGCT
GTTTCATCTCATGAAGGCTC

Product: phospholipase D/transphosphatidylase

Products: NA

Alternate protein names: CL synthase [H]

Number of amino acids: Translated: 467; Mature: 467

Protein sequence:

>467_residues
MIFSIAVIAAIATASHAIIHKRDPRSATIWLLVVALVPLGGSLCYLLFGINRVNRKAMRLRAARPAKSELSNLGSAGMDL
PPHLASLARLGRDVSGKELLPGNRCTPLFGGTEAYPQMLQAICEAKHSIALASYIFEGKGIGALFVDTLAQARSRGVSVK
VLIDDAYARLSNSDAYGALRLHGIDVALFNPPVIPARLHGLHLRNHRKLLIVDGYRGFTGGMNIHRPYWCPKAPESAFRD
LHLEVSGPVVGQMRETFERDWYDSKEEELDESFWTGKSGGPITDGFSLARGIESGPDETLDRMRWIFIGAINAAQKKISI
RTPYFLPDQPTIAALGAAALRGVRVEIVVPRNSDHLFVQWASKAHYWQIIEHGVCIYERPGPFDHGKLMIIDDAWVCVGS
ANWDARSLRLNFEFNLEIYDRRLALELGDDFARIRSASKKVRPTDLSDMPYFVKLRNGFARLFSPIL

Sequences:

>Translated_467_residues
MIFSIAVIAAIATASHAIIHKRDPRSATIWLLVVALVPLGGSLCYLLFGINRVNRKAMRLRAARPAKSELSNLGSAGMDL
PPHLASLARLGRDVSGKELLPGNRCTPLFGGTEAYPQMLQAICEAKHSIALASYIFEGKGIGALFVDTLAQARSRGVSVK
VLIDDAYARLSNSDAYGALRLHGIDVALFNPPVIPARLHGLHLRNHRKLLIVDGYRGFTGGMNIHRPYWCPKAPESAFRD
LHLEVSGPVVGQMRETFERDWYDSKEEELDESFWTGKSGGPITDGFSLARGIESGPDETLDRMRWIFIGAINAAQKKISI
RTPYFLPDQPTIAALGAAALRGVRVEIVVPRNSDHLFVQWASKAHYWQIIEHGVCIYERPGPFDHGKLMIIDDAWVCVGS
ANWDARSLRLNFEFNLEIYDRRLALELGDDFARIRSASKKVRPTDLSDMPYFVKLRNGFARLFSPIL
>Mature_467_residues
MIFSIAVIAAIATASHAIIHKRDPRSATIWLLVVALVPLGGSLCYLLFGINRVNRKAMRLRAARPAKSELSNLGSAGMDL
PPHLASLARLGRDVSGKELLPGNRCTPLFGGTEAYPQMLQAICEAKHSIALASYIFEGKGIGALFVDTLAQARSRGVSVK
VLIDDAYARLSNSDAYGALRLHGIDVALFNPPVIPARLHGLHLRNHRKLLIVDGYRGFTGGMNIHRPYWCPKAPESAFRD
LHLEVSGPVVGQMRETFERDWYDSKEEELDESFWTGKSGGPITDGFSLARGIESGPDETLDRMRWIFIGAINAAQKKISI
RTPYFLPDQPTIAALGAAALRGVRVEIVVPRNSDHLFVQWASKAHYWQIIEHGVCIYERPGPFDHGKLMIIDDAWVCVGS
ANWDARSLRLNFEFNLEIYDRRLALELGDDFARIRSASKKVRPTDLSDMPYFVKLRNGFARLFSPIL

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=472, Percent_Identity=28.3898305084746, Blast_Score=161, Evalue=7e-41,
Organism=Escherichia coli, GI1787007, Length=350, Percent_Identity=28, Blast_Score=119, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022924
- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.-

Molecular weight: Translated: 51851; Mature: 51851

Theoretical pI: Translated: 9.39; Mature: 9.39

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFSIAVIAAIATASHAIIHKRDPRSATIWLLVVALVPLGGSLCYLLFGINRVNRKAMRL
CEEHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
RAARPAKSELSNLGSAGMDLPPHLASLARLGRDVSGKELLPGNRCTPLFGGTEAYPQMLQ
HHCCCCHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
AICEAKHSIALASYIFEGKGIGALFVDTLAQARSRGVSVKVLIDDAYARLSNSDAYGALR
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEEEEEEECHHHHCCCCCCCEEEE
LHGIDVALFNPPVIPARLHGLHLRNHRKLLIVDGYRGFTGGMNIHRPYWCPKAPESAFRD
EEEEEEEEECCCCCCHHHCCEEECCCCEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHE
LHLEVSGPVVGQMRETFERDWYDSKEEELDESFWTGKSGGPITDGFSLARGIESGPDETL
EEEEECCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCCHHHH
DRMRWIFIGAINAAQKKISIRTPYFLPDQPTIAALGAAALRGVRVEIVVPRNSDHLFVQW
HHEEEEEEEECHHHHHEEEEECCEECCCCCHHHHHHHHHHCCEEEEEEEECCCCEEEEEE
ASKAHYWQIIEHGVCIYERPGPFDHGKLMIIDDAWVCVGSANWDARSLRLNFEFNLEIYD
CCCCHHHHHHHCCEEEEECCCCCCCCEEEEEECCEEEEECCCCCCEEEEEEEEEEEEEEE
RRLALELGDDFARIRSASKKVRPTDLSDMPYFVKLRNGFARLFSPIL
EEEEEECCCHHHHHHHHCCCCCCCCCCCCCEEEEHHHHHHHHHHCCC
>Mature Secondary Structure
MIFSIAVIAAIATASHAIIHKRDPRSATIWLLVVALVPLGGSLCYLLFGINRVNRKAMRL
CEEHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
RAARPAKSELSNLGSAGMDLPPHLASLARLGRDVSGKELLPGNRCTPLFGGTEAYPQMLQ
HHCCCCHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
AICEAKHSIALASYIFEGKGIGALFVDTLAQARSRGVSVKVLIDDAYARLSNSDAYGALR
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEEEEEEECHHHHCCCCCCCEEEE
LHGIDVALFNPPVIPARLHGLHLRNHRKLLIVDGYRGFTGGMNIHRPYWCPKAPESAFRD
EEEEEEEEECCCCCCHHHCCEEECCCCEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHE
LHLEVSGPVVGQMRETFERDWYDSKEEELDESFWTGKSGGPITDGFSLARGIESGPDETL
EEEEECCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCCHHHH
DRMRWIFIGAINAAQKKISIRTPYFLPDQPTIAALGAAALRGVRVEIVVPRNSDHLFVQW
HHEEEEEEEECHHHHHEEEEECCEECCCCCHHHHHHHHHHCCEEEEEEEECCCCEEEEEE
ASKAHYWQIIEHGVCIYERPGPFDHGKLMIIDDAWVCVGSANWDARSLRLNFEFNLEIYD
CCCCHHHHHHHCCEEEEECCCCCCCCEEEEEECCEEEEECCCCCCEEEEEEEEEEEEEEE
RRLALELGDDFARIRSASKKVRPTDLSDMPYFVKLRNGFARLFSPIL
EEEEEECCCHHHHHHHHCCCCCCCCCCCCCEEEEHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9443601 [H]