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The map label for this gene is gcvA [H]

Identifier: 103485861

GI number: 103485861

Start: 377378

End: 378283

Strand: Direct

Name: gcvA [H]

Synonym: Sala_0366

Alternate gene names: 103485861

Gene position: 377378-378283 (Clockwise)

Preceding gene: 103485859

Following gene: 103485862

Centisome position: 11.28

GC content: 71.52

Gene sequence:

>906_bases
ATGACTCAACCTGATTCATCTATAGCGCAGCTGCGCCGCCTGCCGCCGCTCGCCGCCCTGCGCGCGTTCGAAGCCGCCGC
GCGCCATGTCAGCTTTCGGCAGGCGGCGGAGGAGCTGGGGGTGACGCCGACGGCGATCAGCCACCAGGTCCGGCTGCTGG
AGGACAGCCTGGGATTCGCGCTGTTCGTGCGGCGCGCGCGCGGCGTCGTGCTGACCGACGCGGGGCGGCGGCTGTTCCCG
ACCCTGCGCGACGGCTTCGATGCGTTCGAGCGCGCGATCTGGGAATTGTCGCCGCGCCCGCGCCGCGTCGCGGTGACGTT
GAGCGCGACGACGCTGTTCACCGTGCGCCGCATCCTGCCCGCGATCGGCCGGTTCCGCGAACGTTTCCCGGACTATGACC
TGCGCCTCCATGCCTCCGACGATCCGGTCGACCTGATCGCCGGGGAGGCCGATGTCGCGGTCCGTTATGGATCGGGCGCC
TTCCGCGGGTTGGTCGCCACGCCCCTGCTGGCCGAGCGCTTCGGCGTCCTATGCAGCCCCAAGATGGCGATTGCGCGGCC
GAACGATCTGCGCGGCGCCACCTTGCTGCACATCGAATGGCGGCGCGCGGGCAAGGCGCCCGACTGGCGCCGGTGGGCGC
GGCTTGCGGGGATCGAGGGGCTGGCGGTCGATCGGGGACCGCGCTTCACCGAGGATGATCATGCGCTGCAAGCCGCGGCG
GCGGGCAGCGGCGTGGTCTTGTCGGGGCTGGCGCTCGCGCAGCCGACGATCGACGCCGGGCTGCTCGTCCACCCGTTCGG
CCCGGTGATCGAGGGCGAAGCCTATCATGTCGTCACGACACCCGACAACCGCGACGCGCCGGCGGTGTGTGCGGTCTGCG
ACTGGCTGCGCGAGGACGTCGTTTAG

Upstream 100 bases:

>100_bases
ATTCAATTCAAACATGGTGAAATCCTTTTCATCCATAGGAGAGGTGCTATCATGAATAATATGGATTATTTCAGTAGGTC
GAACACGAATCCGTCATCTT

Downstream 100 bases:

>100_bases
CGCGCAATCGGCCTGCGCTTGCGCGGAACGCTATTAAGGTCTAAACCGCCGCCGGATGGCCGCCCCGCGAGGGGCGGCCC
TTATTATTTCCGCTTTTGGA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 301; Mature: 300

Protein sequence:

>301_residues
MTQPDSSIAQLRRLPPLAALRAFEAAARHVSFRQAAEELGVTPTAISHQVRLLEDSLGFALFVRRARGVVLTDAGRRLFP
TLRDGFDAFERAIWELSPRPRRVAVTLSATTLFTVRRILPAIGRFRERFPDYDLRLHASDDPVDLIAGEADVAVRYGSGA
FRGLVATPLLAERFGVLCSPKMAIARPNDLRGATLLHIEWRRAGKAPDWRRWARLAGIEGLAVDRGPRFTEDDHALQAAA
AGSGVVLSGLALAQPTIDAGLLVHPFGPVIEGEAYHVVTTPDNRDAPAVCAVCDWLREDVV

Sequences:

>Translated_301_residues
MTQPDSSIAQLRRLPPLAALRAFEAAARHVSFRQAAEELGVTPTAISHQVRLLEDSLGFALFVRRARGVVLTDAGRRLFP
TLRDGFDAFERAIWELSPRPRRVAVTLSATTLFTVRRILPAIGRFRERFPDYDLRLHASDDPVDLIAGEADVAVRYGSGA
FRGLVATPLLAERFGVLCSPKMAIARPNDLRGATLLHIEWRRAGKAPDWRRWARLAGIEGLAVDRGPRFTEDDHALQAAA
AGSGVVLSGLALAQPTIDAGLLVHPFGPVIEGEAYHVVTTPDNRDAPAVCAVCDWLREDVV
>Mature_300_residues
TQPDSSIAQLRRLPPLAALRAFEAAARHVSFRQAAEELGVTPTAISHQVRLLEDSLGFALFVRRARGVVLTDAGRRLFPT
LRDGFDAFERAIWELSPRPRRVAVTLSATTLFTVRRILPAIGRFRERFPDYDLRLHASDDPVDLIAGEADVAVRYGSGAF
RGLVATPLLAERFGVLCSPKMAIARPNDLRGATLLHIEWRRAGKAPDWRRWARLAGIEGLAVDRGPRFTEDDHALQAAAA
GSGVVLSGLALAQPTIDAGLLVHPFGPVIEGEAYHVVTTPDNRDAPAVCAVCDWLREDVV

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=291, Percent_Identity=38.1443298969072, Blast_Score=190, Evalue=1e-49,
Organism=Escherichia coli, GI1788706, Length=292, Percent_Identity=32.1917808219178, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1786448, Length=293, Percent_Identity=31.740614334471, Blast_Score=114, Evalue=9e-27,
Organism=Escherichia coli, GI1786401, Length=164, Percent_Identity=33.5365853658537, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI145693105, Length=170, Percent_Identity=29.4117647058824, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1787128, Length=261, Percent_Identity=26.8199233716475, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI157672245, Length=175, Percent_Identity=33.7142857142857, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI87081978, Length=178, Percent_Identity=30.3370786516854, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI1788748, Length=185, Percent_Identity=30.2702702702703, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1787589, Length=152, Percent_Identity=30.9210526315789, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32902; Mature: 32771

Theoretical pI: Translated: 8.52; Mature: 8.52

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQPDSSIAQLRRLPPLAALRAFEAAARHVSFRQAAEELGVTPTAISHQVRLLEDSLGFA
CCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
LFVRRARGVVLTDAGRRLFPTLRDGFDAFERAIWELSPRPRRVAVTLSATTLFTVRRILP
HHHHHHCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEHHHHHHHHHHHH
AIGRFRERFPDYDLRLHASDDPVDLIAGEADVAVRYGSGAFRGLVATPLLAERFGVLCSP
HHHHHHHHCCCCCEEEECCCCCCEEEECCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCC
KMAIARPNDLRGATLLHIEWRRAGKAPDWRRWARLAGIEGLAVDRGPRFTEDDHALQAAA
CEEECCCCCCCCEEEEEEEHHCCCCCCCHHHHHHHHCCCCEEECCCCCCCCCHHHHHHHH
AGSGVVLSGLALAQPTIDAGLLVHPFGPVIEGEAYHVVTTPDNRDAPAVCAVCDWLREDV
CCCCCEECCHHHHCCCCCCCEEEECCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHC
V
C
>Mature Secondary Structure 
TQPDSSIAQLRRLPPLAALRAFEAAARHVSFRQAAEELGVTPTAISHQVRLLEDSLGFA
CCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
LFVRRARGVVLTDAGRRLFPTLRDGFDAFERAIWELSPRPRRVAVTLSATTLFTVRRILP
HHHHHHCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEHHHHHHHHHHHH
AIGRFRERFPDYDLRLHASDDPVDLIAGEADVAVRYGSGAFRGLVATPLLAERFGVLCSP
HHHHHHHHCCCCCEEEECCCCCCEEEECCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCC
KMAIARPNDLRGATLLHIEWRRAGKAPDWRRWARLAGIEGLAVDRGPRFTEDDHALQAAA
CEEECCCCCCCCEEEEEEEHHCCCCCCCHHHHHHHHCCCCEEECCCCCCCCCHHHHHHHH
AGSGVVLSGLALAQPTIDAGLLVHPFGPVIEGEAYHVVTTPDNRDAPAVCAVCDWLREDV
CCCCCEECCHHHHCCCCCCCEEEECCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHC
V
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]