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The map label for this gene is yngF [H]

Identifier: 103485719

GI number: 103485719

Start: 231846

End: 232649

Strand: Direct

Name: yngF [H]

Synonym: Sala_0223

Alternate gene names: 103485719

Gene position: 231846-232649 (Clockwise)

Preceding gene: 103485718

Following gene: 103485720

Centisome position: 6.93

GC content: 67.79

Gene sequence:

>804_bases
ATGAAGGACCGGATTTCGATCACCATGCTCGACGGCGGGATCGCCGACGTCCGGCTGATCCGCGCCGACAAGATGAATGC
GCTCGACAGCGCAATGTGGGAAGCACTGGCCGAAGCGGTCGATCAGCTGAAGGCGACGGCGGGGCTGCGCGTCGTCGTCC
TGTCGGGCGAGGGGCGCGCCTTTTGCGCGGGCCTCGACCTTTCGAGCCTCGGCGGCGATCGCGACCCCGGCGCGAGCAGC
GCGGGCGGGGGGCTCGCCGAACGGACACGGGGCATCGCCAATAATGCCCAATATGCCGCCTGGGGCTGGCGCGAGCTGCC
TGTGCCGGTGATCGCGGCGGTGCACGGCGTCGCCTTTGGCGCGGGCAGCCAGATCATGGCCGCCGCCGACATCCGCATCG
TCCACCCCGACACGCGCATCGCGATCATGGAGATGCGCTGGGGCCTCGTCCCCGACGTGGCGGGGATGGCGCTGTGGCGC
ACGCAGGTCGCCGACGATGTGCTGCGCGAGCTTGTCTACACCAACCGCGAGTTCACCGGGTCGGAGGCGAAGCTGCTTGG
CTTTGCGACGCATGTGTCGGACGACCCGCTGGCCAAGGCGATGGAACTCGCCGCGGTGATTGCCGACAAGAACCCGCACG
CGATCCGCGGCGCCAAGCGCCTCTGCAACATGCTCGGCGATGCGAGCGACGCCGAAATCCTGCAGGCCGAAAGCGACGAG
CAGGTCAAGCTCATCCGCACCCCGAACCAGATCGAGGCGGTGATGGCGCATATGGAAAAGCGCAGGCCCAATTTTGTCGA
CTAG

Upstream 100 bases:

>100_bases
CGCCGACGATCTTCGTCGATCGCGATGACATGTATTTCGGCAACGACCAGCTTCCGCTTGTCGAGGCGGCTCTAAAACAT
AAAACGCAGAGGACTCTCGC

Downstream 100 bases:

>100_bases
CTGTTTGGTCCCAGCATGTGATGGTGTAGACTTGTCGCCGTTGCAGCGGAGGGAGCTATGGGACAGATTTTACACGGGAG
CGCCAAGACCACGCACGCCA

Product: enoyl-CoA hydratase

Products: trans-2-enoyl-CoA; trans-3-enoyl-CoA; H2O

Alternate protein names: NA

Number of amino acids: Translated: 267; Mature: 267

Protein sequence:

>267_residues
MKDRISITMLDGGIADVRLIRADKMNALDSAMWEALAEAVDQLKATAGLRVVVLSGEGRAFCAGLDLSSLGGDRDPGASS
AGGGLAERTRGIANNAQYAAWGWRELPVPVIAAVHGVAFGAGSQIMAAADIRIVHPDTRIAIMEMRWGLVPDVAGMALWR
TQVADDVLRELVYTNREFTGSEAKLLGFATHVSDDPLAKAMELAAVIADKNPHAIRGAKRLCNMLGDASDAEILQAESDE
QVKLIRTPNQIEAVMAHMEKRRPNFVD

Sequences:

>Translated_267_residues
MKDRISITMLDGGIADVRLIRADKMNALDSAMWEALAEAVDQLKATAGLRVVVLSGEGRAFCAGLDLSSLGGDRDPGASS
AGGGLAERTRGIANNAQYAAWGWRELPVPVIAAVHGVAFGAGSQIMAAADIRIVHPDTRIAIMEMRWGLVPDVAGMALWR
TQVADDVLRELVYTNREFTGSEAKLLGFATHVSDDPLAKAMELAAVIADKNPHAIRGAKRLCNMLGDASDAEILQAESDE
QVKLIRTPNQIEAVMAHMEKRRPNFVD
>Mature_267_residues
MKDRISITMLDGGIADVRLIRADKMNALDSAMWEALAEAVDQLKATAGLRVVVLSGEGRAFCAGLDLSSLGGDRDPGASS
AGGGLAERTRGIANNAQYAAWGWRELPVPVIAAVHGVAFGAGSQIMAAADIRIVHPDTRIAIMEMRWGLVPDVAGMALWR
TQVADDVLRELVYTNREFTGSEAKLLGFATHVSDDPLAKAMELAAVIADKNPHAIRGAKRLCNMLGDASDAEILQAESDE
QVKLIRTPNQIEAVMAHMEKRRPNFVD

Specific function: Catalyzes The Reversible Dehydration Of L-Carnitinyl-CoA To Crotobetainyl-CoA. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI70995211, Length=265, Percent_Identity=29.0566037735849, Blast_Score=91, Evalue=8e-19,
Organism=Homo sapiens, GI4502327, Length=260, Percent_Identity=28.8461538461538, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI31542718, Length=261, Percent_Identity=27.9693486590038, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI68989263, Length=217, Percent_Identity=24.8847926267281, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI221142681, Length=270, Percent_Identity=28.1481481481481, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1787659, Length=246, Percent_Identity=26.4227642276423, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI87082183, Length=263, Percent_Identity=23.9543726235741, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1787660, Length=264, Percent_Identity=27.2727272727273, Blast_Score=62, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17536985, Length=261, Percent_Identity=28.3524904214559, Blast_Score=111, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI17540714, Length=237, Percent_Identity=29.957805907173, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17534483, Length=260, Percent_Identity=26.5384615384615, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17554946, Length=252, Percent_Identity=25.7936507936508, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI25145438, Length=257, Percent_Identity=25.2918287937743, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17560910, Length=204, Percent_Identity=26.4705882352941, Blast_Score=68, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24653139, Length=274, Percent_Identity=29.9270072992701, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI19920382, Length=220, Percent_Identity=30.4545454545455, Blast_Score=94, Evalue=8e-20,
Organism=Drosophila melanogaster, GI20129971, Length=256, Percent_Identity=24.21875, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24653477, Length=256, Percent_Identity=24.21875, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI19922422, Length=255, Percent_Identity=25.0980392156863, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: 4.2.1.17

Molecular weight: Translated: 28625; Mature: 28625

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDRISITMLDGGIADVRLIRADKMNALDSAMWEALAEAVDQLKATAGLRVVVLSGEGRA
CCCEEEEEEECCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCE
FCAGLDLSSLGGDRDPGASSAGGGLAERTRGIANNAQYAAWGWRELPVPVIAAVHGVAFG
EEECCCHHHCCCCCCCCCCCCCCCHHHHHHCCCCCCCEEECCCCCCCHHHHHHHHHHHCC
AGSQIMAAADIRIVHPDTRIAIMEMRWGLVPDVAGMALWRTQVADDVLRELVYTNREFTG
CCCCEEEEECEEEECCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
SEAKLLGFATHVSDDPLAKAMELAAVIADKNPHAIRGAKRLCNMLGDASDAEILQAESDE
CCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCHHEECCCCC
QVKLIRTPNQIEAVMAHMEKRRPNFVD
CEEEEECCHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKDRISITMLDGGIADVRLIRADKMNALDSAMWEALAEAVDQLKATAGLRVVVLSGEGRA
CCCEEEEEEECCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCE
FCAGLDLSSLGGDRDPGASSAGGGLAERTRGIANNAQYAAWGWRELPVPVIAAVHGVAFG
EEECCCHHHCCCCCCCCCCCCCCCHHHHHHCCCCCCCEEECCCCCCCHHHHHHHHHHHCC
AGSQIMAAADIRIVHPDTRIAIMEMRWGLVPDVAGMALWRTQVADDVLRELVYTNREFTG
CCCCEEEEECEEEECCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
SEAKLLGFATHVSDDPLAKAMELAAVIADKNPHAIRGAKRLCNMLGDASDAEILQAESDE
CCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCHHEECCCCC
QVKLIRTPNQIEAVMAHMEKRRPNFVD
CEEEEECCHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (3S)-3-hydroxyacyl-CoA

Specific reaction: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387222; 9384377 [H]