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The map label for this gene is xthA [H]

Identifier: 99080738

GI number: 99080738

Start: 958089

End: 958895

Strand: Reverse

Name: xthA [H]

Synonym: TM1040_0897

Alternate gene names: 99080738

Gene position: 958895-958089 (Counterclockwise)

Preceding gene: 99080739

Following gene: 99080735

Centisome position: 29.96

GC content: 61.96

Gene sequence:

>807_bases
ATGAAGATCGCCAGTTTCAACATCAACGGCATCAAGGCCCGTGTGAACGCCCTGACCGACTGGCTCGACGAGGCCCAGCC
CGATGTGGCAGTGCTGCAGGAAATCAAATCGGTGGATGAGGCGTTCCCGCGCGAGCTCTTCGAAGAGCGCGGCTACAACG
TGGAAACCCACGGCCAGAAAAGCTTCAATGGTGTGGCGATCCTGTCGAAACTGCCGCTTGAGGATGTGCGCCGGGGCCTG
CCCGGGGACGAGAGCGACGAGCAGGCGCGCTGGATTGAAGCCACGGTGGTGGGCAAACAAGCGCTCCGGATCTGTGGTCT
CTACCTGCCCAATGGCAACCCGGTCGAACTGAACGACGACGGCACGCCGGTTGAGGGTGGCAAATACGCCTATAAACTCG
GTTGGATGGAACGCCTGAAGGCGCGCGCCGCGGAGTTGATGGCAGAAGAGATGCCTGCGCTGATGGCGGGGGATTACAAC
ATCATCCCGCAGGCCGAAGATGCCAAGCGCCCCGAAGCCTGGCGGGAGGATGCACTGCACCGCCCAGAGAGCCGCGCCGC
CTTCCAGCGCATTGTGAACCTTGGCTTCACCGAAGCGTTTCGTACGCGCACACGCGGCCCGGGGCACTATACGTTCTGGG
ACTACCAGGCGGGTGCATGGAACCGGGACGATGGCATCCGCATCGACCATTTCCTGCTTACACCGCAGGCGGCAGACCTG
CTGCGGGACTGCCAGATCGACAAGGATATTCGCGGGCGCGAGAAACCCTCCGATCATGTCCCAATCTGGGTGGATCTCGA
TCTCTGA

Upstream 100 bases:

>100_bases
AACACTGCGCCTCGTGGATGACGTGCAACACATGCCCTGTTGCTTGCGGGAGCGGGCGAATTCGGCGATAGACAGGTGGA
CGCACAGAACGGAGACCGCC

Downstream 100 bases:

>100_bases
ACCCTGAGCTGCGCCGCAGGCGGCCTGACAGGTCGTCAGGCGATGCGCCCTGTCGCACCGGCACGACCGCGCCGTGCACG
CGCGCCCGCGTCTTCCTGCT

Product: exodeoxyribonuclease III

Products: NA

Alternate protein names: EXO III; Exonuclease III [H]

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MKIASFNINGIKARVNALTDWLDEAQPDVAVLQEIKSVDEAFPRELFEERGYNVETHGQKSFNGVAILSKLPLEDVRRGL
PGDESDEQARWIEATVVGKQALRICGLYLPNGNPVELNDDGTPVEGGKYAYKLGWMERLKARAAELMAEEMPALMAGDYN
IIPQAEDAKRPEAWREDALHRPESRAAFQRIVNLGFTEAFRTRTRGPGHYTFWDYQAGAWNRDDGIRIDHFLLTPQAADL
LRDCQIDKDIRGREKPSDHVPIWVDLDL

Sequences:

>Translated_268_residues
MKIASFNINGIKARVNALTDWLDEAQPDVAVLQEIKSVDEAFPRELFEERGYNVETHGQKSFNGVAILSKLPLEDVRRGL
PGDESDEQARWIEATVVGKQALRICGLYLPNGNPVELNDDGTPVEGGKYAYKLGWMERLKARAAELMAEEMPALMAGDYN
IIPQAEDAKRPEAWREDALHRPESRAAFQRIVNLGFTEAFRTRTRGPGHYTFWDYQAGAWNRDDGIRIDHFLLTPQAADL
LRDCQIDKDIRGREKPSDHVPIWVDLDL
>Mature_268_residues
MKIASFNINGIKARVNALTDWLDEAQPDVAVLQEIKSVDEAFPRELFEERGYNVETHGQKSFNGVAILSKLPLEDVRRGL
PGDESDEQARWIEATVVGKQALRICGLYLPNGNPVELNDDGTPVEGGKYAYKLGWMERLKARAAELMAEEMPALMAGDYN
IIPQAEDAKRPEAWREDALHRPESRAAFQRIVNLGFTEAFRTRTRGPGHYTFWDYQAGAWNRDDGIRIDHFLLTPQAADL
LRDCQIDKDIRGREKPSDHVPIWVDLDL

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction [H]

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=275, Percent_Identity=27.2727272727273, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI18375503, Length=275, Percent_Identity=27.2727272727273, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI18375501, Length=275, Percent_Identity=27.2727272727273, Blast_Score=97, Evalue=1e-20,
Organism=Escherichia coli, GI1788046, Length=271, Percent_Identity=33.9483394833948, Blast_Score=138, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI71989536, Length=275, Percent_Identity=27.2727272727273, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI221330655, Length=274, Percent_Identity=27.007299270073, Blast_Score=81, Evalue=8e-16,
Organism=Drosophila melanogaster, GI17136678, Length=274, Percent_Identity=27.007299270073, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 30372; Mature: 30372

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIASFNINGIKARVNALTDWLDEAQPDVAVLQEIKSVDEAFPRELFEERGYNVETHGQK
CEEEEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCC
SFNGVAILSKLPLEDVRRGLPGDESDEQARWIEATVVGKQALRICGLYLPNGNPVELNDD
CCCCEEHHHHCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEECCCCCEEEECCC
GTPVEGGKYAYKLGWMERLKARAAELMAEEMPALMAGDYNIIPQAEDAKRPEAWREDALH
CCCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCCHHHHHHHHC
RPESRAAFQRIVNLGFTEAFRTRTRGPGHYTFWDYQAGAWNRDDGIRIDHFLLTPQAADL
CCHHHHHHHHHHHCCHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCEEEEEEECCHHHHH
LRDCQIDKDIRGREKPSDHVPIWVDLDL
HHHCCCCCCCCCCCCCCCCCCEEEEECC
>Mature Secondary Structure
MKIASFNINGIKARVNALTDWLDEAQPDVAVLQEIKSVDEAFPRELFEERGYNVETHGQK
CEEEEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCC
SFNGVAILSKLPLEDVRRGLPGDESDEQARWIEATVVGKQALRICGLYLPNGNPVELNDD
CCCCEEHHHHCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEECCCCCEEEECCC
GTPVEGGKYAYKLGWMERLKARAAELMAEEMPALMAGDYNIIPQAEDAKRPEAWREDALH
CCCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCCHHHHHHHHC
RPESRAAFQRIVNLGFTEAFRTRTRGPGHYTFWDYQAGAWNRDDGIRIDHFLLTPQAADL
CCHHHHHHHHHHHCCHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCEEEEEEECCHHHHH
LRDCQIDKDIRGREKPSDHVPIWVDLDL
HHHCCCCCCCCCCCCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]