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The map label for this gene is kdgA [H]

Identifier: 94972279

GI number: 94972279

Start: 212717

End: 213349

Strand: Direct

Name: kdgA [H]

Synonym: Dgeo_2816

Alternate gene names: 94972279

Gene position: 212717-213349 (Clockwise)

Preceding gene: 94972280

Following gene: 94972278

Centisome position: 37.05

GC content: 66.98

Gene sequence:

>633_bases
ATGCGACCCCGCCTGACCGACGTGCTGGCTGCTGACCGCCTGCTGCCCCTCTTTACACCGGAGCCAGGAGAGACGGCAGC
TAGGCGCCTGACAATTCTGAAGCGAGCTGGGATCCGGGCGGTGGAACTCACCCACCGCAGCCCACAGACCCTGGCGGCCT
TCCGCACGCTGCGTGGCCATTTCCCCGAAGTGCTGCTGGGTGCCGGAACGGTGCTGAGCGCGGCAGATGCCGAAGCGTTT
GCACAGGCTGGCGCCGACTTCATCGTGAGCCCCTGCTGGATCCCAGCGGTGGCCCGAGTCTGCCAGGAACGTGGCCTGCC
GTATCTCCCCGGCGCGGGCACGGTGCGCGAGGTGTTCGAGGCCCAGCAGGAAGGTGCGGAAATCGTCAAACTGTTTCCCG
GTGAGGTGCTTGGTCCGGCGTTTGTGCGGGCGCTCCTGGGTCCGCTCCCTCACAGCCGGGTGCTGGTCACAGGCGGCGTG
CAGCCCACTCGCCAGAGCGTGGGCGAATGGCTCCAGGCAGGTGCCCTGGCGGTCGGTCTCGGCAGTCAGCTGTTCAAACT
GAACGGAGACACACTGAAGGCCCAGCTGACGGACCTCTTGCGCTGGACTCAGGCGGAAAGCAGCCATGGCTGA

Upstream 100 bases:

>100_bases
CGGTGCCCGACCATCACGAGCTGTACCGAGCGGCCACCCAGCGGATGCGGGTCCTGGGAGCCGCGTTGGACAGGTTGCGA
AAGGCGCAGGAGGTGCCCAC

Downstream 100 bases:

>100_bases
AGCCCCCACGAGCGTTCTGACCTTTGGGGAAGCCCTGCTGAAATTGGTTCTGCTCCCCACTCAACGGTTGGAGAGCATGC
AGACCCTAGACGCGCAGTGT

Product: 2-dehydro-3-deoxyphosphogluconate aldolase/4-hydroxy-2-oxoglutarate aldolase

Products: NA

Alternate protein names: 4-hydroxy-2-oxoglutarate aldolase; 2-keto-4-hydroxyglutarate aldolase; KHG-aldolase; 2-dehydro-3-deoxy-phosphogluconate aldolase; 2-keto-3-deoxy-6-phosphogluconate aldolase; KDPG-aldolase; Phospho-2-dehydro-3-deoxygluconate aldolase; Phospho-2-keto-3-deoxygluconate aldolase [H]

Number of amino acids: Translated: 210; Mature: 210

Protein sequence:

>210_residues
MRPRLTDVLAADRLLPLFTPEPGETAARRLTILKRAGIRAVELTHRSPQTLAAFRTLRGHFPEVLLGAGTVLSAADAEAF
AQAGADFIVSPCWIPAVARVCQERGLPYLPGAGTVREVFEAQQEGAEIVKLFPGEVLGPAFVRALLGPLPHSRVLVTGGV
QPTRQSVGEWLQAGALAVGLGSQLFKLNGDTLKAQLTDLLRWTQAESSHG

Sequences:

>Translated_210_residues
MRPRLTDVLAADRLLPLFTPEPGETAARRLTILKRAGIRAVELTHRSPQTLAAFRTLRGHFPEVLLGAGTVLSAADAEAF
AQAGADFIVSPCWIPAVARVCQERGLPYLPGAGTVREVFEAQQEGAEIVKLFPGEVLGPAFVRALLGPLPHSRVLVTGGV
QPTRQSVGEWLQAGALAVGLGSQLFKLNGDTLKAQLTDLLRWTQAESSHG
>Mature_210_residues
MRPRLTDVLAADRLLPLFTPEPGETAARRLTILKRAGIRAVELTHRSPQTLAAFRTLRGHFPEVLLGAGTVLSAADAEAF
AQAGADFIVSPCWIPAVARVCQERGLPYLPGAGTVREVFEAQQEGAEIVKLFPGEVLGPAFVRALLGPLPHSRVLVTGGV
QPTRQSVGEWLQAGALAVGLGSQLFKLNGDTLKAQLTDLLRWTQAESSHG

Specific function: KEY ENZYME IN THE ENTNER-DOUDOROFF PATHWAY. PARTICIPATES IN THE REGULATION OF THE INTRACELLULAR LEVEL OF GLYOXYLATE. [C]

COG id: COG0800

COG function: function code G; 2-keto-3-deoxy-6-phosphogluconate aldolase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the KHG/KDPG aldolase family [H]

Homologues:

Organism=Escherichia coli, GI48994954, Length=153, Percent_Identity=35.2941176470588, Blast_Score=87, Evalue=8e-19,
Organism=Escherichia coli, GI1788156, Length=164, Percent_Identity=32.3170731707317, Blast_Score=72, Evalue=2e-14,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000887
- InterPro:   IPR013785 [H]

Pfam domain/function: PF01081 Aldolase [H]

EC number: =4.1.3.16; =4.1.2.14 [H]

Molecular weight: Translated: 22414; Mature: 22414

Theoretical pI: Translated: 8.79; Mature: 8.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPRLTDVLAADRLLPLFTPEPGETAARRLTILKRAGIRAVELTHRSPQTLAAFRTLRGH
CCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHC
FPEVLLGAGTVLSAADAEAFAQAGADFIVSPCWIPAVARVCQERGLPYLPGAGTVREVFE
CHHHHHHCCHHHHHHHHHHHHHCCCCCEECCHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
AQQEGAEIVKLFPGEVLGPAFVRALLGPLPHSRVLVTGGVQPTRQSVGEWLQAGALAVGL
HHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHCHHHHHC
GSQLFKLNGDTLKAQLTDLLRWTQAESSHG
CCHHHHCCCCHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRPRLTDVLAADRLLPLFTPEPGETAARRLTILKRAGIRAVELTHRSPQTLAAFRTLRGH
CCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHC
FPEVLLGAGTVLSAADAEAFAQAGADFIVSPCWIPAVARVCQERGLPYLPGAGTVREVFE
CHHHHHHCCHHHHHHHHHHHHHCCCCCEECCHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
AQQEGAEIVKLFPGEVLGPAFVRALLGPLPHSRVLVTGGVQPTRQSVGEWLQAGALAVGL
HHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHCHHHHHC
GSQLFKLNGDTLKAQLTDLLRWTQAESSHG
CCHHHHCCCCHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8760912; 9384377 [H]