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The map label for this gene is ntrB [H]

Identifier: 93006540

GI number: 93006540

Start: 2124004

End: 2125392

Strand: Direct

Name: ntrB [H]

Synonym: Pcryo_1716

Alternate gene names: 93006540

Gene position: 2124004-2125392 (Clockwise)

Preceding gene: 93006527

Following gene: 93006541

Centisome position: 69.41

GC content: 42.12

Gene sequence:

>1389_bases
ATGACCGCTGATTTTATAAGCGCAAAACCAACGCCTGACTTAACCTTTATATCGCAGCATTTGTTCACGGCTATCTTGTG
GGTCAATGATGAATTGTCTATTACTTGGCTAAATGCCCAAGCTGAGCAGCTACTGGCGATCAGTAGTGGACGATTGCTTA
ATCAGTCGGTATTGATGCTGCTTGCGCCCGAAGAGTTGAAAACCAAACGCAGCACACTTATTGACAGTTCAAAAGACAAA
ACCTGCTCTTTGACTGAACGTTTTTTTCAAGCGAAGCAGTACCAACAACCTTTTATTGACCATGACCATCTGATAAACAG
CCCATTAAATGGCAACTTATCACTCTCGATTGACTATAGCGTCACCCCTGTTATCTATCAGCAGCAAAATTACTTTATTA
TAGAGATATGGGGCAAAGACCGCCAAAGTCGCATCTCAGAAGAACAGCGCCAACAGCAGCAGTACAATGTTGCGCGTCAT
ATGCTGCGCTCGGTTGCCCATGAAATCAAAAATCCGTTGGCTGGTATTCGCGGTGCCGCGCAATTATTGCAGCGTCAATT
TATCAAATTTAGTGAGCGCTCTTTTAATACTCACACTACTGATACTGACTACCCTAGCCTTATATCGAGCCCGACTTTAA
ACTCTGATAACCATCTACAGAAAACCGCTGACAAATTACGCAGCTATACCGACATCATTATCTCAGAGACAGATCGTCTA
ACTCAGCTGATTGGGCAGTTTCTTGGTTCTAATCAATTACCACATTGGCAAATGCTCAATGTTCATGAGCCAATAGAGCA
TGTTTTGGCATTGATCATCCATCAATATCCGCAAGTTTTATTACAGCGTGACTATGACTTATCGTTACCTGAACTCTGTG
CTGATAAAGACCAGCTGATACAAGTCTTCTTAAACTTAATTAATAATGCTTGCGAATCCATGACGGAATTTGCACAAACA
CTGCAGCAAAGCGTGCCAGCCGTATCCAGTCAATTAACATCAGATAGTCCACTTAATAATCTTGATTTTGACGATGGCTA
TCAACCCAAACTCCATATTCAAACACGTGTTGCCTTTCAACATACGATTGCCGGGCAGCAACATAAACAAGTACTGCAAG
TTGCTATCACCGATAATGGTTCTGGCATTCATCCTGATATCATTGGGCAGATATTCTTCCCTATGGTGACCAGTCGCGCG
CTAGGAACTGGGCTTGGTTTATCCATTGTGCAAGATATTATCAGCCGTCATCATGGTATGATTGATGTCAGCTCTACTCA
ATCGCAGGGCGCCAGTACAAATACTAATGTCGATAATAAAAATAGCCATTATAGTCAAACCCGTTTTACCCTTTATTTGC
CTTTTCATCAGCCAATTGCTGAAAATTAA

Upstream 100 bases:

>100_bases
ATTGTACGTTTTTTTTACAAAAATCGCCAGTTTTGCCGCTCTATAGTGTTGATTTATGGTTAAATATTATATCGAGCCTC
TATTTATCTAGGTATTACTC

Downstream 100 bases:

>100_bases
TGCCTTCACCTCAATTCTTCTCACTGCTTATTAGGCTAAATACATGACCCAGTATAATGACACCCAATCTCTTACAAGCA
ATGAGCGAACGCCAATGGTC

Product: signal transduction histidine kinase, nitrogen specific, NtrB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 462; Mature: 461

Protein sequence:

>462_residues
MTADFISAKPTPDLTFISQHLFTAILWVNDELSITWLNAQAEQLLAISSGRLLNQSVLMLLAPEELKTKRSTLIDSSKDK
TCSLTERFFQAKQYQQPFIDHDHLINSPLNGNLSLSIDYSVTPVIYQQQNYFIIEIWGKDRQSRISEEQRQQQQYNVARH
MLRSVAHEIKNPLAGIRGAAQLLQRQFIKFSERSFNTHTTDTDYPSLISSPTLNSDNHLQKTADKLRSYTDIIISETDRL
TQLIGQFLGSNQLPHWQMLNVHEPIEHVLALIIHQYPQVLLQRDYDLSLPELCADKDQLIQVFLNLINNACESMTEFAQT
LQQSVPAVSSQLTSDSPLNNLDFDDGYQPKLHIQTRVAFQHTIAGQQHKQVLQVAITDNGSGIHPDIIGQIFFPMVTSRA
LGTGLGLSIVQDIISRHHGMIDVSSTQSQGASTNTNVDNKNSHYSQTRFTLYLPFHQPIAEN

Sequences:

>Translated_462_residues
MTADFISAKPTPDLTFISQHLFTAILWVNDELSITWLNAQAEQLLAISSGRLLNQSVLMLLAPEELKTKRSTLIDSSKDK
TCSLTERFFQAKQYQQPFIDHDHLINSPLNGNLSLSIDYSVTPVIYQQQNYFIIEIWGKDRQSRISEEQRQQQQYNVARH
MLRSVAHEIKNPLAGIRGAAQLLQRQFIKFSERSFNTHTTDTDYPSLISSPTLNSDNHLQKTADKLRSYTDIIISETDRL
TQLIGQFLGSNQLPHWQMLNVHEPIEHVLALIIHQYPQVLLQRDYDLSLPELCADKDQLIQVFLNLINNACESMTEFAQT
LQQSVPAVSSQLTSDSPLNNLDFDDGYQPKLHIQTRVAFQHTIAGQQHKQVLQVAITDNGSGIHPDIIGQIFFPMVTSRA
LGTGLGLSIVQDIISRHHGMIDVSSTQSQGASTNTNVDNKNSHYSQTRFTLYLPFHQPIAEN
>Mature_461_residues
TADFISAKPTPDLTFISQHLFTAILWVNDELSITWLNAQAEQLLAISSGRLLNQSVLMLLAPEELKTKRSTLIDSSKDKT
CSLTERFFQAKQYQQPFIDHDHLINSPLNGNLSLSIDYSVTPVIYQQQNYFIIEIWGKDRQSRISEEQRQQQQYNVARHM
LRSVAHEIKNPLAGIRGAAQLLQRQFIKFSERSFNTHTTDTDYPSLISSPTLNSDNHLQKTADKLRSYTDIIISETDRLT
QLIGQFLGSNQLPHWQMLNVHEPIEHVLALIIHQYPQVLLQRDYDLSLPELCADKDQLIQVFLNLINNACESMTEFAQTL
QQSVPAVSSQLTSDSPLNNLDFDDGYQPKLHIQTRVAFQHTIAGQQHKQVLQVAITDNGSGIHPDIIGQIFFPMVTSRAL
GTGLGLSIVQDIISRHHGMIDVSSTQSQGASTNTNVDNKNSHYSQTRFTLYLPFHQPIAEN

Specific function: NtrB acts as a signal transducer which responds to the nitrogen level of cell and modulates the activity of ntrC. In nitrogen limitation ntrB activates ntrC by phosphorylating it, while in nitrogen excess ntrC is dephosphorylated and consequently inactiva

COG id: COG3852

COG function: function code T; Signal transduction histidine kinase, nitrogen specific

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790300, Length=452, Percent_Identity=29.8672566371681, Blast_Score=169, Evalue=4e-43,
Organism=Escherichia coli, GI1788549, Length=325, Percent_Identity=25.5384615384615, Blast_Score=85, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 52341; Mature: 52210

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTADFISAKPTPDLTFISQHLFTAILWVNDELSITWLNAQAEQLLAISSGRLLNQSVLML
CCCCCCCCCCCCCHHHHHHHHHHHHHEECCCEEEEEECCCHHHHHHHHCCCHHCCCCEEE
LAPEELKTKRSTLIDSSKDKTCSLTERFFQAKQYQQPFIDHDHLINSPLNGNLSLSIDYS
ECCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCEEEEEECE
VTPVIYQQQNYFIIEIWGKDRQSRISEEQRQQQQYNVARHMLRSVAHEIKNPLAGIRGAA
ECEEEEECCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QLLQRQFIKFSERSFNTHTTDTDYPSLISSPTLNSDNHLQKTADKLRSYTDIIISETDRL
HHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHH
TQLIGQFLGSNQLPHWQMLNVHEPIEHVLALIIHQYPQVLLQRDYDLSLPELCADKDQLI
HHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCHHHHH
QVFLNLINNACESMTEFAQTLQQSVPAVSSQLTSDSPLNNLDFDDGYQPKLHIQTRVAFQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEHHHHH
HTIAGQQHKQVLQVAITDNGSGIHPDIIGQIFFPMVTSRALGTGLGLSIVQDIISRHHGM
HHHCCHHHHEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCE
IDVSSTQSQGASTNTNVDNKNSHYSQTRFTLYLPFHQPIAEN
EEECCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCC
>Mature Secondary Structure 
TADFISAKPTPDLTFISQHLFTAILWVNDELSITWLNAQAEQLLAISSGRLLNQSVLML
CCCCCCCCCCCCHHHHHHHHHHHHHEECCCEEEEEECCCHHHHHHHHCCCHHCCCCEEE
LAPEELKTKRSTLIDSSKDKTCSLTERFFQAKQYQQPFIDHDHLINSPLNGNLSLSIDYS
ECCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCEEEEEECE
VTPVIYQQQNYFIIEIWGKDRQSRISEEQRQQQQYNVARHMLRSVAHEIKNPLAGIRGAA
ECEEEEECCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QLLQRQFIKFSERSFNTHTTDTDYPSLISSPTLNSDNHLQKTADKLRSYTDIIISETDRL
HHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHH
TQLIGQFLGSNQLPHWQMLNVHEPIEHVLALIIHQYPQVLLQRDYDLSLPELCADKDQLI
HHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCHHHHH
QVFLNLINNACESMTEFAQTLQQSVPAVSSQLTSDSPLNNLDFDDGYQPKLHIQTRVAFQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEHHHHH
HTIAGQQHKQVLQVAITDNGSGIHPDIIGQIFFPMVTSRALGTGLGLSIVQDIISRHHGM
HHHCCHHHHEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCE
IDVSSTQSQGASTNTNVDNKNSHYSQTRFTLYLPFHQPIAEN
EEECCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2574025 [H]