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The map label for this gene is glnG [H]

Identifier: 93006541

GI number: 93006541

Start: 2125436

End: 2127148

Strand: Direct

Name: glnG [H]

Synonym: Pcryo_1717

Alternate gene names: 93006541

Gene position: 2125436-2127148 (Clockwise)

Preceding gene: 93006540

Following gene: 93006543

Centisome position: 69.46

GC content: 45.71

Gene sequence:

>1713_bases
ATGACCCAGTATAATGACACCCAATCTCTTACAAGCAATGAGCGAACGCCAATGGTCACGGTCAATAACAGTCCTGATAA
TGAGCCTGCCACTTTGTGGCTCATTGATGATGATGCGGCGCTGCGTATGGTACTTGCAGATACTTTTGAAGACGCTGGAT
TGACGGTTATCAGCTTTACCCAAGCACAAGCAGCATGGACACGCCTCAATGATATTCTAGAATCGTCAGAGTCAATCGCC
TTGTTACCTGATGTGATATTGACCGATATTCGTATGCCGATGATGGATGGTCTGTCGTTTAGTGACTGGGTACACAAGCA
CTTTCCTAAGCTTCCGATTGTCATTATGACAGCGCATTCAGACCTTACTTCTGCCATTAGCAGCTATCAGACTGGTGCAT
TTGAATATCTGCCCAAGCCGTTTGATTTAGATGATGCGGTCGCAACGATTTATAAAGCCATTAATTATCAGCCGAATCTA
GTCATATCAAACCATTCGATCGAGTCTAATACGAATGTAAGTACCGATACCAAAGAACAGCCTGTGAACAAACCAAATGC
AGCCAAATCAGCTAGAAAAACCCCTAAAGCTGCTGAAAGTAAAACTCATGAAAAACCCAAACCCCAAACCAATAACAATC
CAAGCGGCATCATTGGTCAATCACAAGCCATGCAAACGGTATTTCGTGCCATTGGTAGACTTGCGCACTCACCGATTACC
GTTTTAATCACTGGTGAATCAGGTACAGGTAAAGAGTTGGTCGCTAGCGCTTTACATCAGCACTCACCGCGACACAATAA
AACCTTTATTGCACTAAATATGGCAGCAATTCCCCATGATTTGATTGAGTCTGAATTGTTTGGTCATGAAAAAGGCGCAT
TCACTGGCGCCACAACTTCACGCCAAGGTCGTTTTGAGCAAGCAGATGGCGGCACGTTATTTTTAGATGAAATCGGTGAC
ATGCCTTTTAGTACCCAAACGCGGCTGCTACGAGTGCTGGCAAATGGCGAATTTTTTCGCGTGGGTGGTCAGCAGCCCGT
CAAAGTAGATGTACGCATTATTGCCGCTACCCATCAAAACTTGGAAGAATTGGTCAAACAGGGTAAATTCCGTGAGGATC
TATTTTATCGCCTGAATGTCATTCGCCTGCCATTGCCGCCCTTACGGACTCGCCGCGAGGACGTACCAGCATTAACCACT
TATTTTATGCAGCGCGCTGCTGAGCAGATGAATACGGCACCAAAACAGTTACATCCGGCAGCAATGCAAATGATGCAGTC
TTTTGAATGGCGCGGTAATGTCCGTCAACTTGAGAATGTCTGTCTCTGGCTGACAGTAATGGCGACTGGCGACACCGTTA
TGATCGATGACTTGCCTCCAGAGTTGCTCGAAAACATCTCCTCTACTCTAAACAATCAACAGCAGCATGTATCCTCACAA
GACGCTATTGAAAATAATCATCTACAAAGTCAGGATACTTTTGGCACCCAAAGCTGGCAACAAGCTTTAGGTATTTGGGC
GAAGCAGTCACTACAAACTGGCGCAACCGATATTTTACAGACAGCAACACCTGAGTTTGAACGGGTCTTGCTAACCGCAG
CGCTCCATCATAGCGGCGGCAAAAAGATAGCTGCTGCCAATTTACTTGGCTGGGGACGCAATACGCTGACACGTAAATTA
CAACAGCTGAACCTCTCATCATATCAAGAGTAA

Upstream 100 bases:

>100_bases
AGTCAAACCCGTTTTACCCTTTATTTGCCTTTTCATCAGCCAATTGCTGAAAATTAATGCCTTCACCTCAATTCTTCTCA
CTGCTTATTAGGCTAAATAC

Downstream 100 bases:

>100_bases
TATTAGTGTGAATAAAGTGATAAAAAATATGAATTAAAAAATCAATAATATCAATTATTTATGATATTCAGCAAAAAAAA
TGGCTAAATAATTAAAAATA

Product: nitrogen metabolism transcriptional regulator, NtrC, Fis family

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 570; Mature: 569

Protein sequence:

>570_residues
MTQYNDTQSLTSNERTPMVTVNNSPDNEPATLWLIDDDAALRMVLADTFEDAGLTVISFTQAQAAWTRLNDILESSESIA
LLPDVILTDIRMPMMDGLSFSDWVHKHFPKLPIVIMTAHSDLTSAISSYQTGAFEYLPKPFDLDDAVATIYKAINYQPNL
VISNHSIESNTNVSTDTKEQPVNKPNAAKSARKTPKAAESKTHEKPKPQTNNNPSGIIGQSQAMQTVFRAIGRLAHSPIT
VLITGESGTGKELVASALHQHSPRHNKTFIALNMAAIPHDLIESELFGHEKGAFTGATTSRQGRFEQADGGTLFLDEIGD
MPFSTQTRLLRVLANGEFFRVGGQQPVKVDVRIIAATHQNLEELVKQGKFREDLFYRLNVIRLPLPPLRTRREDVPALTT
YFMQRAAEQMNTAPKQLHPAAMQMMQSFEWRGNVRQLENVCLWLTVMATGDTVMIDDLPPELLENISSTLNNQQQHVSSQ
DAIENNHLQSQDTFGTQSWQQALGIWAKQSLQTGATDILQTATPEFERVLLTAALHHSGGKKIAAANLLGWGRNTLTRKL
QQLNLSSYQE

Sequences:

>Translated_570_residues
MTQYNDTQSLTSNERTPMVTVNNSPDNEPATLWLIDDDAALRMVLADTFEDAGLTVISFTQAQAAWTRLNDILESSESIA
LLPDVILTDIRMPMMDGLSFSDWVHKHFPKLPIVIMTAHSDLTSAISSYQTGAFEYLPKPFDLDDAVATIYKAINYQPNL
VISNHSIESNTNVSTDTKEQPVNKPNAAKSARKTPKAAESKTHEKPKPQTNNNPSGIIGQSQAMQTVFRAIGRLAHSPIT
VLITGESGTGKELVASALHQHSPRHNKTFIALNMAAIPHDLIESELFGHEKGAFTGATTSRQGRFEQADGGTLFLDEIGD
MPFSTQTRLLRVLANGEFFRVGGQQPVKVDVRIIAATHQNLEELVKQGKFREDLFYRLNVIRLPLPPLRTRREDVPALTT
YFMQRAAEQMNTAPKQLHPAAMQMMQSFEWRGNVRQLENVCLWLTVMATGDTVMIDDLPPELLENISSTLNNQQQHVSSQ
DAIENNHLQSQDTFGTQSWQQALGIWAKQSLQTGATDILQTATPEFERVLLTAALHHSGGKKIAAANLLGWGRNTLTRKL
QQLNLSSYQE
>Mature_569_residues
TQYNDTQSLTSNERTPMVTVNNSPDNEPATLWLIDDDAALRMVLADTFEDAGLTVISFTQAQAAWTRLNDILESSESIAL
LPDVILTDIRMPMMDGLSFSDWVHKHFPKLPIVIMTAHSDLTSAISSYQTGAFEYLPKPFDLDDAVATIYKAINYQPNLV
ISNHSIESNTNVSTDTKEQPVNKPNAAKSARKTPKAAESKTHEKPKPQTNNNPSGIIGQSQAMQTVFRAIGRLAHSPITV
LITGESGTGKELVASALHQHSPRHNKTFIALNMAAIPHDLIESELFGHEKGAFTGATTSRQGRFEQADGGTLFLDEIGDM
PFSTQTRLLRVLANGEFFRVGGQQPVKVDVRIIAATHQNLEELVKQGKFREDLFYRLNVIRLPLPPLRTRREDVPALTTY
FMQRAAEQMNTAPKQLHPAAMQMMQSFEWRGNVRQLENVCLWLTVMATGDTVMIDDLPPELLENISSTLNNQQQHVSSQD
AIENNHLQSQDTFGTQSWQQALGIWAKQSLQTGATDILQTATPEFERVLLTAALHHSGGKKIAAANLLGWGRNTLTRKLQ
QLNLSSYQE

Specific function: Member of the two-component regulatory system ntrB/ntrC involved in the activation of nitrogen assimilatory genes such as glnA. NtrC is phosphorylated by ntrB and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=534, Percent_Identity=52.6217228464419, Blast_Score=538, Evalue=1e-154,
Organism=Escherichia coli, GI1788550, Length=431, Percent_Identity=39.6751740139211, Blast_Score=318, Evalue=4e-88,
Organism=Escherichia coli, GI1790437, Length=479, Percent_Identity=38.2045929018789, Blast_Score=309, Evalue=3e-85,
Organism=Escherichia coli, GI1788905, Length=463, Percent_Identity=35.4211663066955, Blast_Score=269, Evalue=3e-73,
Organism=Escherichia coli, GI87082117, Length=273, Percent_Identity=47.6190476190476, Blast_Score=256, Evalue=2e-69,
Organism=Escherichia coli, GI1789233, Length=351, Percent_Identity=40.4558404558405, Blast_Score=255, Evalue=6e-69,
Organism=Escherichia coli, GI1789087, Length=250, Percent_Identity=46.4, Blast_Score=234, Evalue=9e-63,
Organism=Escherichia coli, GI1786524, Length=362, Percent_Identity=40.3314917127072, Blast_Score=223, Evalue=3e-59,
Organism=Escherichia coli, GI87082152, Length=244, Percent_Identity=46.3114754098361, Blast_Score=219, Evalue=5e-58,
Organism=Escherichia coli, GI1787583, Length=225, Percent_Identity=47.5555555555556, Blast_Score=201, Evalue=8e-53,
Organism=Escherichia coli, GI87081872, Length=258, Percent_Identity=42.6356589147287, Blast_Score=194, Evalue=9e-51,
Organism=Escherichia coli, GI1789828, Length=276, Percent_Identity=33.3333333333333, Blast_Score=141, Evalue=1e-34,
Organism=Escherichia coli, GI87081858, Length=335, Percent_Identity=27.4626865671642, Blast_Score=126, Evalue=5e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR010114
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 63301; Mature: 63170

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQYNDTQSLTSNERTPMVTVNNSPDNEPATLWLIDDDAALRMVLADTFEDAGLTVISFT
CCCCCCCHHHCCCCCCCEEEECCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCEEEEEEH
QAQAAWTRLNDILESSESIALLPDVILTDIRMPMMDGLSFSDWVHKHFPKLPIVIMTAHS
HHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEEECCC
DLTSAISSYQTGAFEYLPKPFDLDDAVATIYKAINYQPNLVISNHSIESNTNVSTDTKEQ
HHHHHHHHHCCCCHHHCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCC
PVNKPNAAKSARKTPKAAESKTHEKPKPQTNNNPSGIIGQSQAMQTVFRAIGRLAHSPIT
CCCCCCHHHHHHCCCCHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEE
VLITGESGTGKELVASALHQHSPRHNKTFIALNMAAIPHDLIESELFGHEKGAFTGATTS
EEEECCCCCCHHHHHHHHHHCCCCCCCEEEEEEEHHCCHHHHHHHHHCCCCCCCCCCCCC
RQGRFEQADGGTLFLDEIGDMPFSTQTRLLRVLANGEFFRVGGQQPVKVDVRIIAATHQN
CCCCCCCCCCCEEEHHHHCCCCCCHHHHHHHHHHCCCEEEECCCCCEEEEEEEEEHHHHH
LEELVKQGKFREDLFYRLNVIRLPLPPLRTRREDVPALTTYFMQRAAEQMNTAPKQLHPA
HHHHHHCCCHHHHHHHHEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHCCHH
AMQMMQSFEWRGNVRQLENVCLWLTVMATGDTVMIDDLPPELLENISSTLNNQQQHVSSQ
HHHHHHHHHHCCCHHHHHHHEEEEEEEECCCEEEECCCCHHHHHHHHHHHCHHHHHCCHH
DAIENNHLQSQDTFGTQSWQQALGIWAKQSLQTGATDILQTATPEFERVLLTAALHHSGG
HHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHCCCC
KKIAAANLLGWGRNTLTRKLQQLNLSSYQE
CEEEEHHHHCCCHHHHHHHHHHCCHHHCCC
>Mature Secondary Structure 
TQYNDTQSLTSNERTPMVTVNNSPDNEPATLWLIDDDAALRMVLADTFEDAGLTVISFT
CCCCCCHHHCCCCCCCEEEECCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCEEEEEEH
QAQAAWTRLNDILESSESIALLPDVILTDIRMPMMDGLSFSDWVHKHFPKLPIVIMTAHS
HHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEEECCC
DLTSAISSYQTGAFEYLPKPFDLDDAVATIYKAINYQPNLVISNHSIESNTNVSTDTKEQ
HHHHHHHHHCCCCHHHCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCC
PVNKPNAAKSARKTPKAAESKTHEKPKPQTNNNPSGIIGQSQAMQTVFRAIGRLAHSPIT
CCCCCCHHHHHHCCCCHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEE
VLITGESGTGKELVASALHQHSPRHNKTFIALNMAAIPHDLIESELFGHEKGAFTGATTS
EEEECCCCCCHHHHHHHHHHCCCCCCCEEEEEEEHHCCHHHHHHHHHCCCCCCCCCCCCC
RQGRFEQADGGTLFLDEIGDMPFSTQTRLLRVLANGEFFRVGGQQPVKVDVRIIAATHQN
CCCCCCCCCCCEEEHHHHCCCCCCHHHHHHHHHHCCCEEEECCCCCEEEEEEEEEHHHHH
LEELVKQGKFREDLFYRLNVIRLPLPPLRTRREDVPALTTYFMQRAAEQMNTAPKQLHPA
HHHHHHCCCHHHHHHHHEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHCCHH
AMQMMQSFEWRGNVRQLENVCLWLTVMATGDTVMIDDLPPELLENISSTLNNQQQHVSSQ
HHHHHHHHHHCCCHHHHHHHEEEEEEEECCCEEEECCCCHHHHHHHHHHHCHHHHHCCHH
DAIENNHLQSQDTFGTQSWQQALGIWAKQSLQTGATDILQTATPEFERVLLTAALHHSGG
HHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHCCCC
KKIAAANLLGWGRNTLTRKLQQLNLSSYQE
CEEEEHHHHCCCHHHHHHHHHHCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]