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The map label for this gene is tgt [H]

Identifier: 93006527

GI number: 93006527

Start: 2107566

End: 2108717

Strand: Direct

Name: tgt [H]

Synonym: Pcryo_1703

Alternate gene names: 93006527

Gene position: 2107566-2108717 (Clockwise)

Preceding gene: 93006525

Following gene: 93006540

Centisome position: 68.88

GC content: 44.27

Gene sequence:

>1152_bases
ATGCAATTTGTCTTACATAAAACAGCCAGTGGTGTCACTCGCGCACGCCGTGGTACAGTCCACCTCAATCATGGCGATGT
ACAAACGCCAGCCTTTATGCCAGTAGGTACTTACGGTACCGTTAAAGGCATGCTACCACGTGATATTGAAGCAATTGGCG
CAGATATCATCTTGGGCAATACTTTTCATTTATGGTTACGCCCTGGTACCGACATTATTGATAAATTCGGTGGCTTACAT
AAATTCATGCATTGGGATAAGCCTATTTTGACTGACTCTGGCGGATTTCAGGTATTCAGTCTTGGCGCGATGCGTAAGAT
CACTGAAGAAGGTGTGACTTTTAAATCACCTATCGATGGTGCTAAAGTCTTTTTATCACCAGAAAAATCGATGCAGATTC
AATATAGCTTGAACTCAGATATCGTGATGCAGTTTGATGAGTGTACGCCCTATCCTGCCACTCACGATGAAGCTAAAAAG
TCTTTAGAGTTGTCACTACGTTGGGGACAACGCTGCGTTGATGAGCATAAAAATCTTGGCAGTACCAATGCGTTATTTGG
CATCATTCAAGGCAGTATGTACGCTGATTTACGCAAGCAATCACTAGAAGGCTTGCTTGAAATTGGCTTTGATGGCTATG
CCATTGGCGGTTTGTCTGTTGGCGAGCCAAAAGAAGAGATGATAGACGTCCTAGATTATATCGCAGATGACATGCCTGCT
GATAAGCCGCGCTATCTGATGGGTGTCGGTAAGCCTGAAGATCTCGTTGAAGGAGTACGCCGCGGTGTCGATATGTTCGA
TTGTGTCATGCCAACCCGGAATGCGCGTAATGGTCATTATTTTGTCACAGGCGACGCCGACAATGCGGGCGTAGTACGTA
TACGCAATAGCCAATACCGCACCGATGAAGGTCCATTAGACCCTGAATGCGATTGCTATACTTGCCAGAATTTTAGTCGT
GCTTATTTATCGCATTTGAATAAATGTAAAGAGATGCTTGGCGCGCAGTTAGCGACGATTCATAACTTACGTTACTATCA
ACGTTTGATGCAAAATATCAGAGATGCCATCGAACAAGATAAATTTGATGAGTTCGTGAATGAATTTTATAGCAAACGCG
GACAGACGGTGCCAGAGCTGAATTTGCGTTAA

Upstream 100 bases:

>100_bases
ACACCCCATGAAATCTAAGCTAAATAAACATCAATAACCAAAAAAAGCCTAACAATGCTATAATTAGCACCATTTTCATA
GCTCATATAGCGGTTACCTT

Downstream 100 bases:

>100_bases
TAGTAATTTGACATTAGCTATTCAGATATAAAATACAAAAAAAGGCTTAAGATCAACCTTAAGCCTTTTTTGTTCACTAA
ATTTTGACAAGCTAACTATC

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 383; Mature: 383

Protein sequence:

>383_residues
MQFVLHKTASGVTRARRGTVHLNHGDVQTPAFMPVGTYGTVKGMLPRDIEAIGADIILGNTFHLWLRPGTDIIDKFGGLH
KFMHWDKPILTDSGGFQVFSLGAMRKITEEGVTFKSPIDGAKVFLSPEKSMQIQYSLNSDIVMQFDECTPYPATHDEAKK
SLELSLRWGQRCVDEHKNLGSTNALFGIIQGSMYADLRKQSLEGLLEIGFDGYAIGGLSVGEPKEEMIDVLDYIADDMPA
DKPRYLMGVGKPEDLVEGVRRGVDMFDCVMPTRNARNGHYFVTGDADNAGVVRIRNSQYRTDEGPLDPECDCYTCQNFSR
AYLSHLNKCKEMLGAQLATIHNLRYYQRLMQNIRDAIEQDKFDEFVNEFYSKRGQTVPELNLR

Sequences:

>Translated_383_residues
MQFVLHKTASGVTRARRGTVHLNHGDVQTPAFMPVGTYGTVKGMLPRDIEAIGADIILGNTFHLWLRPGTDIIDKFGGLH
KFMHWDKPILTDSGGFQVFSLGAMRKITEEGVTFKSPIDGAKVFLSPEKSMQIQYSLNSDIVMQFDECTPYPATHDEAKK
SLELSLRWGQRCVDEHKNLGSTNALFGIIQGSMYADLRKQSLEGLLEIGFDGYAIGGLSVGEPKEEMIDVLDYIADDMPA
DKPRYLMGVGKPEDLVEGVRRGVDMFDCVMPTRNARNGHYFVTGDADNAGVVRIRNSQYRTDEGPLDPECDCYTCQNFSR
AYLSHLNKCKEMLGAQLATIHNLRYYQRLMQNIRDAIEQDKFDEFVNEFYSKRGQTVPELNLR
>Mature_383_residues
MQFVLHKTASGVTRARRGTVHLNHGDVQTPAFMPVGTYGTVKGMLPRDIEAIGADIILGNTFHLWLRPGTDIIDKFGGLH
KFMHWDKPILTDSGGFQVFSLGAMRKITEEGVTFKSPIDGAKVFLSPEKSMQIQYSLNSDIVMQFDECTPYPATHDEAKK
SLELSLRWGQRCVDEHKNLGSTNALFGIIQGSMYADLRKQSLEGLLEIGFDGYAIGGLSVGEPKEEMIDVLDYIADDMPA
DKPRYLMGVGKPEDLVEGVRRGVDMFDCVMPTRNARNGHYFVTGDADNAGVVRIRNSQYRTDEGPLDPECDCYTCQNFSR
AYLSHLNKCKEMLGAQLATIHNLRYYQRLMQNIRDAIEQDKFDEFVNEFYSKRGQTVPELNLR

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=357, Percent_Identity=46.218487394958, Blast_Score=323, Evalue=1e-88,
Organism=Escherichia coli, GI1786607, Length=382, Percent_Identity=65.9685863874346, Blast_Score=533, Evalue=1e-153,
Organism=Caenorhabditis elegans, GI17544680, Length=355, Percent_Identity=41.9718309859155, Blast_Score=292, Evalue=2e-79,
Organism=Caenorhabditis elegans, GI25146502, Length=172, Percent_Identity=29.6511627906977, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI19920510, Length=368, Percent_Identity=42.6630434782609, Blast_Score=326, Evalue=2e-89,
Organism=Drosophila melanogaster, GI21355311, Length=266, Percent_Identity=25.5639097744361, Blast_Score=96, Evalue=5e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 43098; Mature: 43098

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQFVLHKTASGVTRARRGTVHLNHGDVQTPAFMPVGTYGTVKGMLPRDIEAIGADIILGN
CCEEEECHHHHHHHHHCCEEEECCCCCCCCCEEECCCCCCCCCCCCHHHHHHCCEEEECC
TFHLWLRPGTDIIDKFGGLHKFMHWDKPILTDSGGFQVFSLGAMRKITEEGVTFKSPIDG
EEEEEECCCHHHHHHHHHHHHHHHCCCCEEECCCCEEEEEHHHHHHHHHCCCEECCCCCC
AKVFLSPEKSMQIQYSLNSDIVMQFDECTPYPATHDEAKKSLELSLRWGQRCVDEHKNLG
CEEEECCCCCEEEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
STNALFGIIQGSMYADLRKQSLEGLLEIGFDGYAIGGLSVGEPKEEMIDVLDYIADDMPA
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHHHHCCCC
DKPRYLMGVGKPEDLVEGVRRGVDMFDCVMPTRNARNGHYFVTGDADNAGVVRIRNSQYR
CCCCEEEECCCHHHHHHHHHCCCHHHHHHCCCCCCCCCCEEEEECCCCCCEEEECCCCCC
TDEGPLDPECDCYTCQNFSRAYLSHLNKCKEMLGAQLATIHNLRYYQRLMQNIRDAIEQD
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KFDEFVNEFYSKRGQTVPELNLR
HHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MQFVLHKTASGVTRARRGTVHLNHGDVQTPAFMPVGTYGTVKGMLPRDIEAIGADIILGN
CCEEEECHHHHHHHHHCCEEEECCCCCCCCCEEECCCCCCCCCCCCHHHHHHCCEEEECC
TFHLWLRPGTDIIDKFGGLHKFMHWDKPILTDSGGFQVFSLGAMRKITEEGVTFKSPIDG
EEEEEECCCHHHHHHHHHHHHHHHCCCCEEECCCCEEEEEHHHHHHHHHCCCEECCCCCC
AKVFLSPEKSMQIQYSLNSDIVMQFDECTPYPATHDEAKKSLELSLRWGQRCVDEHKNLG
CEEEECCCCCEEEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
STNALFGIIQGSMYADLRKQSLEGLLEIGFDGYAIGGLSVGEPKEEMIDVLDYIADDMPA
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHHHHCCCC
DKPRYLMGVGKPEDLVEGVRRGVDMFDCVMPTRNARNGHYFVTGDADNAGVVRIRNSQYR
CCCCEEEECCCHHHHHHHHHCCCHHHHHHCCCCCCCCCCEEEEECCCCCCEEEECCCCCC
TDEGPLDPECDCYTCQNFSRAYLSHLNKCKEMLGAQLATIHNLRYYQRLMQNIRDAIEQD
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KFDEFVNEFYSKRGQTVPELNLR
HHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA