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The map label for this gene is yfcG [H]

Identifier: 93006082

GI number: 93006082

Start: 1524266

End: 1524955

Strand: Reverse

Name: yfcG [H]

Synonym: Pcryo_1254

Alternate gene names: 93006082

Gene position: 1524955-1524266 (Counterclockwise)

Preceding gene: 93006083

Following gene: 93006081

Centisome position: 49.84

GC content: 43.62

Gene sequence:

>690_bases
ATGCTCAAATTTTATTTTCATCAAACCCCAAACCCGATGAAAGTAGCTGTCTATCTCGAAGAGACTGCCTTGCCGTATGA
GTTGATCGCTATAGATACCTTAAAAGGCGAACAACATAGCCCTGAGTATCACGCTATTAACCCCAATGGTAAAACTCCGG
CAATCGAAGATGATGGCAAACGCGTGTTTGATTCTACTGCTATCTTGATGTATCTCTCCGAAAAAACAGGGAAATTAGCC
GGTAAACCAGAAGATCGTAGCGAGATGCTGTCATGGTTGATGTTTATAGCGACAGGTTTAGGACCGTTTTCTGGTCAATC
CGTGCATTTTAGACATAAGGCACCAGAGAAAACGCCTTATGCTATCAATCGCTATCTTCGTGAAGCCGAACGTCATTACG
AAGTGTTAGAGACCCACATGGAAGGTCGCGAGTATCTGGTTGGCGATGAGTATTCCATTGCTGATATTTCAGCTTGGGGG
TGGATTGATAAAGCAACCGTAGTCTTAGGTGAGGAAGGTTTAGACAACTATCCTAATCTAAAGCGCTGGTTTGCAAATAT
AAGTGCTCGTCCTGCAGTCCAAAAAGCCCGAAATATTCCTAAAGGCATCGAGTTTAAGACCGAGTTTGATGATGTGGCAG
CACGCGCGCTATATCCTCAAAACTTTGCTAAACCCGATAAGAATACCTAA

Upstream 100 bases:

>100_bases
AAATAATCCTTAATGACTGCATGAGTCTTTAAGGATTATTTGTATTTTTCATTGCTTTTATGATTGTCAAACTAAGACCT
CAACACGATAGGATCAGGTT

Downstream 100 bases:

>100_bases
CAGCCAAAGAACGACTATCAAGTTAGAGGAATAATTGGCATAGGGTAAGAGTACGAAATATGATACTCACATGATTAAGA
TTGGTGACGGCGATCCATTC

Product: glutathione S-transferase-like protein

Products: HX; R-S-glutathione

Alternate protein names: GST-like protein yfcG [H]

Number of amino acids: Translated: 229; Mature: 229

Protein sequence:

>229_residues
MLKFYFHQTPNPMKVAVYLEETALPYELIAIDTLKGEQHSPEYHAINPNGKTPAIEDDGKRVFDSTAILMYLSEKTGKLA
GKPEDRSEMLSWLMFIATGLGPFSGQSVHFRHKAPEKTPYAINRYLREAERHYEVLETHMEGREYLVGDEYSIADISAWG
WIDKATVVLGEEGLDNYPNLKRWFANISARPAVQKARNIPKGIEFKTEFDDVAARALYPQNFAKPDKNT

Sequences:

>Translated_229_residues
MLKFYFHQTPNPMKVAVYLEETALPYELIAIDTLKGEQHSPEYHAINPNGKTPAIEDDGKRVFDSTAILMYLSEKTGKLA
GKPEDRSEMLSWLMFIATGLGPFSGQSVHFRHKAPEKTPYAINRYLREAERHYEVLETHMEGREYLVGDEYSIADISAWG
WIDKATVVLGEEGLDNYPNLKRWFANISARPAVQKARNIPKGIEFKTEFDDVAARALYPQNFAKPDKNT
>Mature_229_residues
MLKFYFHQTPNPMKVAVYLEETALPYELIAIDTLKGEQHSPEYHAINPNGKTPAIEDDGKRVFDSTAILMYLSEKTGKLA
GKPEDRSEMLSWLMFIATGLGPFSGQSVHFRHKAPEKTPYAINRYLREAERHYEVLETHMEGREYLVGDEYSIADISAWG
WIDKATVVLGEEGLDNYPNLKRWFANISARPAVQKARNIPKGIEFKTEFDDVAARALYPQNFAKPDKNT

Specific function: Has disulfide bond reductase activity (in vitro). Has low hydroperoxidase activity with cumene hydroperoxide. Has very low glutathione-S-transferase activity (in vitro) [H]

COG id: COG0625

COG function: function code O; Glutathione S-transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1788640, Length=204, Percent_Identity=44.1176470588235, Blast_Score=160, Evalue=5e-41,
Organism=Escherichia coli, GI87082195, Length=221, Percent_Identity=38.0090497737557, Blast_Score=129, Evalue=1e-31,
Organism=Escherichia coli, GI87081789, Length=203, Percent_Identity=29.064039408867, Blast_Score=71, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6324100, Length=238, Percent_Identity=29.8319327731092, Blast_Score=105, Evalue=5e-24,
Organism=Saccharomyces cerevisiae, GI6322968, Length=207, Percent_Identity=29.4685990338164, Blast_Score=62, Evalue=7e-11,
Organism=Drosophila melanogaster, GI28571670, Length=162, Percent_Identity=30.2469135802469, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI17864592, Length=162, Percent_Identity=30.2469135802469, Blast_Score=74, Evalue=7e-14,
Organism=Drosophila melanogaster, GI17864598, Length=193, Percent_Identity=27.979274611399, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI45549270, Length=192, Percent_Identity=27.6041666666667, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI17864594, Length=156, Percent_Identity=27.5641025641026, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24654992, Length=205, Percent_Identity=27.3170731707317, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI17933730, Length=182, Percent_Identity=26.9230769230769, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24646249, Length=161, Percent_Identity=31.055900621118, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI20130115, Length=192, Percent_Identity=28.125, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI85725204, Length=217, Percent_Identity=27.1889400921659, Blast_Score=67, Evalue=6e-12,
Organism=Drosophila melanogaster, GI17737923, Length=217, Percent_Identity=27.1889400921659, Blast_Score=67, Evalue=6e-12,
Organism=Drosophila melanogaster, GI19922532, Length=208, Percent_Identity=29.8076923076923, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24654983, Length=198, Percent_Identity=29.7979797979798, Blast_Score=64, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: 2.5.1.18

Molecular weight: Translated: 26114; Mature: 26114

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKFYFHQTPNPMKVAVYLEETALPYELIAIDTLKGEQHSPEYHAINPNGKTPAIEDDGK
CEEEEEECCCCCEEEEEEEECCCCCEEEEEEEECCCCCCCCCEEEECCCCCCCCCCCCCC
RVFDSTAILMYLSEKTGKLAGKPEDRSEMLSWLMFIATGLGPFSGQSVHFRHKAPEKTPY
CHHCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCHH
AINRYLREAERHYEVLETHMEGREYLVGDEYSIADISAWGWIDKATVVLGEEGLDNYPNL
HHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEEECCCCCCCEEEEECCCCCCCCCCH
KRWFANISARPAVQKARNIPKGIEFKTEFDDVAARALYPQNFAKPDKNT
HHHHHCCCCCHHHHHHHCCCCCCCEECCHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MLKFYFHQTPNPMKVAVYLEETALPYELIAIDTLKGEQHSPEYHAINPNGKTPAIEDDGK
CEEEEEECCCCCEEEEEEEECCCCCEEEEEEEECCCCCCCCCEEEECCCCCCCCCCCCCC
RVFDSTAILMYLSEKTGKLAGKPEDRSEMLSWLMFIATGLGPFSGQSVHFRHKAPEKTPY
CHHCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCHH
AINRYLREAERHYEVLETHMEGREYLVGDEYSIADISAWGWIDKATVVLGEEGLDNYPNL
HHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEEECCCCCCCEEEEECCCCCCCCCCH
KRWFANISARPAVQKARNIPKGIEFKTEFDDVAARALYPQNFAKPDKNT
HHHHHCCCCCHHHHHHHCCCCCCCEECCHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: RX; glutathione

Specific reaction: RX + glutathione = HX + R-S-glutathione

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]