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The map label for this gene is dhmA

Identifier: 93006081

GI number: 93006081

Start: 1523164

End: 1524075

Strand: Reverse

Name: dhmA

Synonym: Pcryo_1253

Alternate gene names: 93006081

Gene position: 1524075-1523164 (Counterclockwise)

Preceding gene: 93006082

Following gene: 93006080

Centisome position: 49.81

GC content: 48.03

Gene sequence:

>912_bases
ATGAAAATACTCAGAACTCCCGATTCGCGTTTTGCAAACCTACCAGATTACAACTTTGACCCGCACTATCTAATGGTCGA
CGACAGCGAAGACAGTGAGCTGCGCGTGCATTATTTAGACGAAGGACCAAGAGACGCTGATCCAGTGTTACTGCTGCATG
GGGAGCCTTCATGGTGTTATCTATATCGTAAAATGATTCCGATACTGACTGCAGCGGGACACCGCGTTATTGCGCCAGAC
CTCCCCGGTTTTGGGCGCTCAGACAAACCCGCGTCTCGTACTGACTATACTTACCAACGTCATGTCAACTGGATGCAATC
CGTACTCGACCAATTAGATCTGAATAACATCACTCTGTTTTGTCAAGATTGGGGTGGACTGATTGGTTTGCGTTTGGTTG
CAGAAAATCCAGACCGATTTGCTCGGGTAGCAGCTGGCAACACCATGCTCCCCACCGGCGACCACGATCTAGGTGAAGGT
TTTCGTAAATGGCAACAATTCTCGCAGGAAATACCTCAATTTCATGTCGGAGGGACCATCAAGAGCGGTACCGTTACCAA
GCTATCACAGGCGGTGATTGACGCCTACAATGCGCCATTTCCTGATGAATCCTATAAGGAGGGTGCTAGACAGTTTCCAC
TGCTTGTACCCTCGACGCCGGATGATCCTGCTTCAGAAAATAATCGCGCCGCATGGATTGAGCTGAGTAAATGGACAAAG
CCCTTTATCACACTATTCAGCGATTCTGACCCAGTGACTGCTGGTGGCGATCGCATTATGCAAAAAATCATTCCCGGAAC
CAAAGGTCAAGCACATACGACTATTGCCAACGGCGGACATTTTCTTCAAGAAGACCAAGGTGAAAAAGTGGCTAAGCTTT
TGGTCCAATTTATTCATGATAACCCGAGATAA

Upstream 100 bases:

>100_bases
CGATCCATTCTCATCGGGTTTTGTGGCGATCCACCCTAACTCTAAGATTCCATCAGCTGTGTAAGAATACGCGCTCATTT
ACCCAACTCAAGGACGAGAC

Downstream 100 bases:

>100_bases
TTCATCATCACCCTAAGTGACTTAAAGTAAAAAATATAAAGAACTTTGCGCTATTTTCTATAATTTTTTGACAGCTTTTG
CTTTTTGTTAGTCATGCTAA

Product: haloalkane dehalogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MKILRTPDSRFANLPDYNFDPHYLMVDDSEDSELRVHYLDEGPRDADPVLLLHGEPSWCYLYRKMIPILTAAGHRVIAPD
LPGFGRSDKPASRTDYTYQRHVNWMQSVLDQLDLNNITLFCQDWGGLIGLRLVAENPDRFARVAAGNTMLPTGDHDLGEG
FRKWQQFSQEIPQFHVGGTIKSGTVTKLSQAVIDAYNAPFPDESYKEGARQFPLLVPSTPDDPASENNRAAWIELSKWTK
PFITLFSDSDPVTAGGDRIMQKIIPGTKGQAHTTIANGGHFLQEDQGEKVAKLLVQFIHDNPR

Sequences:

>Translated_303_residues
MKILRTPDSRFANLPDYNFDPHYLMVDDSEDSELRVHYLDEGPRDADPVLLLHGEPSWCYLYRKMIPILTAAGHRVIAPD
LPGFGRSDKPASRTDYTYQRHVNWMQSVLDQLDLNNITLFCQDWGGLIGLRLVAENPDRFARVAAGNTMLPTGDHDLGEG
FRKWQQFSQEIPQFHVGGTIKSGTVTKLSQAVIDAYNAPFPDESYKEGARQFPLLVPSTPDDPASENNRAAWIELSKWTK
PFITLFSDSDPVTAGGDRIMQKIIPGTKGQAHTTIANGGHFLQEDQGEKVAKLLVQFIHDNPR
>Mature_303_residues
MKILRTPDSRFANLPDYNFDPHYLMVDDSEDSELRVHYLDEGPRDADPVLLLHGEPSWCYLYRKMIPILTAAGHRVIAPD
LPGFGRSDKPASRTDYTYQRHVNWMQSVLDQLDLNNITLFCQDWGGLIGLRLVAENPDRFARVAAGNTMLPTGDHDLGEG
FRKWQQFSQEIPQFHVGGTIKSGTVTKLSQAVIDAYNAPFPDESYKEGARQFPLLVPSTPDDPASENNRAAWIELSKWTK
PFITLFSDSDPVTAGGDRIMQKIIPGTKGQAHTTIANGGHFLQEDQGEKVAKLLVQFIHDNPR

Specific function: Catalyzes hydrolytic cleavage of carbon-halogen bonds in halogenated aliphatic compounds, leading to the formation of the corresponding primary alcohols, halide ions and protons

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the haloalkane dehalogenase family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI27597073, Length=115, Percent_Identity=34.7826086956522, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI218777837, Length=118, Percent_Identity=32.2033898305085, Blast_Score=70, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17562298, Length=115, Percent_Identity=30.4347826086957, Blast_Score=75, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6324392, Length=126, Percent_Identity=30.1587301587302, Blast_Score=69, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DHMA_PSYCK (Q1QBB9)

Other databases:

- EMBL:   CP000323
- RefSeq:   YP_580518.1
- ProteinModelPortal:   Q1QBB9
- SMR:   Q1QBB9
- STRING:   Q1QBB9
- GeneID:   4034282
- GenomeReviews:   CP000323_GR
- KEGG:   pcr:Pcryo_1253
- NMPDR:   fig|335284.3.peg.835
- eggNOG:   COG0596
- HOGENOM:   HBG748003
- OMA:   SYARHVE
- ProtClustDB:   PRK00870
- BioCyc:   PCRY335284:PCRYO_1253-MONOMER
- HAMAP:   MF_01230
- InterPro:   IPR000073
- InterPro:   IPR000639
- PRINTS:   PR00111
- PRINTS:   PR00412

Pfam domain/function: PF00561 Abhydrolase_1

EC number: =3.8.1.5

Molecular weight: Translated: 34045; Mature: 34045

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: NA

Important sites: ACT_SITE 123-123 ACT_SITE 250-250 ACT_SITE 280-280

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILRTPDSRFANLPDYNFDPHYLMVDDSEDSELRVHYLDEGPRDADPVLLLHGEPSWCY
CCCCCCCCHHHCCCCCCCCCCCEEEEECCCCCCEEEEECCCCCCCCCCEEEEECCCCHHH
LYRKMIPILTAAGHRVIAPDLPGFGRSDKPASRTDYTYQRHVNWMQSVLDQLDLNNITLF
HHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEE
CQDWGGLIGLRLVAENPDRFARVAAGNTMLPTGDHDLGEGFRKWQQFSQEIPQFHVGGTI
EECCCCEEEEEEEECCCHHHEEECCCCEECCCCCCHHHHHHHHHHHHHHHCCCEECCCEE
KSGTVTKLSQAVIDAYNAPFPDESYKEGARQFPLLVPSTPDDPASENNRAAWIELSKWTK
CCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCEECCCCCCCCCCCCCCEEEEEECCCCC
PFITLFSDSDPVTAGGDRIMQKIIPGTKGQAHTTIANGGHFLQEDQGEKVAKLLVQFIHD
CEEEEECCCCCCCCCHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHC
NPR
CCC
>Mature Secondary Structure
MKILRTPDSRFANLPDYNFDPHYLMVDDSEDSELRVHYLDEGPRDADPVLLLHGEPSWCY
CCCCCCCCHHHCCCCCCCCCCCEEEEECCCCCCEEEEECCCCCCCCCCEEEEECCCCHHH
LYRKMIPILTAAGHRVIAPDLPGFGRSDKPASRTDYTYQRHVNWMQSVLDQLDLNNITLF
HHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEE
CQDWGGLIGLRLVAENPDRFARVAAGNTMLPTGDHDLGEGFRKWQQFSQEIPQFHVGGTI
EECCCCEEEEEEEECCCHHHEEECCCCEECCCCCCHHHHHHHHHHHHHHHCCCEECCCEE
KSGTVTKLSQAVIDAYNAPFPDESYKEGARQFPLLVPSTPDDPASENNRAAWIELSKWTK
CCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCEECCCCCCCCCCCCCCEEEEEECCCCC
PFITLFSDSDPVTAGGDRIMQKIIPGTKGQAHTTIANGGHFLQEDQGEKVAKLLVQFIHD
CEEEEECCCCCCCCCHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHC
NPR
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA