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The map label for this gene is ispG

Identifier: 93005482

GI number: 93005482

Start: 771206

End: 772321

Strand: Direct

Name: ispG

Synonym: Pcryo_0652

Alternate gene names: 93005482

Gene position: 771206-772321 (Clockwise)

Preceding gene: 93005481

Following gene: 93005483

Centisome position: 25.2

GC content: 44.89

Gene sequence:

>1116_bases
ATGTCAACGTCAGCGCCTATTAACCGTCGTCTTACGAAGAAAATATATGTCGGTGATGTCGCGATTGGTGGTGATGCGCC
GATTAGTGTGCAAAGTATGACCAATACCGATACCTGTGATGTGGCAGCGACTGTTGCCCAAATTGAGCGCTGTGTTGAAG
CAGGCGCTGATTTGATGCGAGTTTCTACTCCGACGATGGATACCGTTAAAGCGTTTGGTGAAATTCGCAAGTTGGTCAGC
GTACCCTTGATAGCAGATGTGCATTTTGACCACAAAATTGCTTTAGCCGTTGCGGCAGCGGGCGCAGATTGTTTACGTAT
TAACCCGGGCAATATCGGTAGTGACGCCAAAGTACGCGAAGTAGTCGCCTGTGCCAAGCATTACAATATTCCTATTCGTA
TCGGCGTCAATGCTGGCTCATTAGAGAAAGACATTCAGCGTAAGTATAAAGAGCCAAATGGCGCAGCGATGCTCGAATCG
GCAATGCGTCATATTGATATATTAGAACGTCTTAATTTTGATCAATATAAAGTATCGGTTAAAGCCAGCAACGTATTTTT
GACCATGGATGCCTATCGTCTGATTTCAGCACAAATTGATAATCCGCTGCATTTAGGGGTGACTGAAGCTGGTGTTTACC
GTACTGGTTCGGTCAAATCTGCTATCGCGCTGGGTGGTTTATTATTAGACGGTATCGGTGATACGATACGTATCTCCTTA
GCTGCAGAGCCAGAAGAAGAAATTAAGATTGGCTTTGATATCTTAAAATCGCTCAATATTCGCTCTAATGGTGTCAATTT
CATTGCTTGTCCAAGCTGCTCACGTCAAGAGTTCGACGTGATTAGAGTGATGACCGCGTTAGAGTCGCGCTTAGAAGACA
TTCGTGAGCCGATGAATTTGTCAGTGATTGGCTGTAAGGTAAATGGTCCAGGTGAAGCGAAAGAAGCCGATATCGGTATT
GTTGGCGCCGCACCAAAGTCTCTGGTCTACCGTATGGGCGAAAAAAGTCATCTGATTGATACAGATAATTTGGTCGATGA
GATAGAAGGTATGGTACGCGCCCATGCAGAAGAATTGGCAAAAAAACGCGAAAACGAGATTATACGTGTTAGATAA

Upstream 100 bases:

>100_bases
GCACCTTAATTTATTAATTGGTTCGGTTTTTAAAGTAAATAGTATTACCTTGTTTTTATTGTTATTCTCGTTTTTACTGC
TTTCATGAAAGGGTTTGTCT

Downstream 100 bases:

>100_bases
TAAAGCGTTTTATTAATAAAAATTATAGAGCTGTTTGTTAAAACTTAAAGGACATAACCGTACCATGATTAAAGCGATTA
AAGGGTTTAATGATATTTTG

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase

Number of amino acids: Translated: 371; Mature: 370

Protein sequence:

>371_residues
MSTSAPINRRLTKKIYVGDVAIGGDAPISVQSMTNTDTCDVAATVAQIERCVEAGADLMRVSTPTMDTVKAFGEIRKLVS
VPLIADVHFDHKIALAVAAAGADCLRINPGNIGSDAKVREVVACAKHYNIPIRIGVNAGSLEKDIQRKYKEPNGAAMLES
AMRHIDILERLNFDQYKVSVKASNVFLTMDAYRLISAQIDNPLHLGVTEAGVYRTGSVKSAIALGGLLLDGIGDTIRISL
AAEPEEEIKIGFDILKSLNIRSNGVNFIACPSCSRQEFDVIRVMTALESRLEDIREPMNLSVIGCKVNGPGEAKEADIGI
VGAAPKSLVYRMGEKSHLIDTDNLVDEIEGMVRAHAEELAKKRENEIIRVR

Sequences:

>Translated_371_residues
MSTSAPINRRLTKKIYVGDVAIGGDAPISVQSMTNTDTCDVAATVAQIERCVEAGADLMRVSTPTMDTVKAFGEIRKLVS
VPLIADVHFDHKIALAVAAAGADCLRINPGNIGSDAKVREVVACAKHYNIPIRIGVNAGSLEKDIQRKYKEPNGAAMLES
AMRHIDILERLNFDQYKVSVKASNVFLTMDAYRLISAQIDNPLHLGVTEAGVYRTGSVKSAIALGGLLLDGIGDTIRISL
AAEPEEEIKIGFDILKSLNIRSNGVNFIACPSCSRQEFDVIRVMTALESRLEDIREPMNLSVIGCKVNGPGEAKEADIGI
VGAAPKSLVYRMGEKSHLIDTDNLVDEIEGMVRAHAEELAKKRENEIIRVR
>Mature_370_residues
STSAPINRRLTKKIYVGDVAIGGDAPISVQSMTNTDTCDVAATVAQIERCVEAGADLMRVSTPTMDTVKAFGEIRKLVSV
PLIADVHFDHKIALAVAAAGADCLRINPGNIGSDAKVREVVACAKHYNIPIRIGVNAGSLEKDIQRKYKEPNGAAMLESA
MRHIDILERLNFDQYKVSVKASNVFLTMDAYRLISAQIDNPLHLGVTEAGVYRTGSVKSAIALGGLLLDGIGDTIRISLA
AEPEEEIKIGFDILKSLNIRSNGVNFIACPSCSRQEFDVIRVMTALESRLEDIREPMNLSVIGCKVNGPGEAKEADIGIV
GAAPKSLVYRMGEKSHLIDTDNLVDEIEGMVRAHAEELAKKRENEIIRVR

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family

Homologues:

Organism=Escherichia coli, GI1788863, Length=370, Percent_Identity=61.0810810810811, Blast_Score=468, Evalue=1e-133,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPG_PSYA2 (Q4FTW7)

Other databases:

- EMBL:   CP000082
- RefSeq:   YP_263975.1
- STRING:   Q4FTW7
- GeneID:   3516142
- GenomeReviews:   CP000082_GR
- KEGG:   par:Psyc_0682
- eggNOG:   COG0821
- HOGENOM:   HBG335271
- OMA:   APIVVQS
- PhylomeDB:   Q4FTW7
- ProtClustDB:   PRK00366
- BioCyc:   PARC259536:PSYC_0682-MONOMER
- HAMAP:   MF_00159
- InterPro:   IPR013785
- InterPro:   IPR011005
- InterPro:   IPR016425
- InterPro:   IPR004588
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF004640
- TIGRFAMs:   TIGR00612

Pfam domain/function: PF04551 GcpE; SSF51717 DHP_synth_like

EC number: =1.17.7.1

Molecular weight: Translated: 40164; Mature: 40033

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTSAPINRRLTKKIYVGDVAIGGDAPISVQSMTNTDTCDVAATVAQIERCVEAGADLMR
CCCCCCCHHHHHHEEEEEEEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCEEE
VSTPTMDTVKAFGEIRKLVSVPLIADVHFDHKIALAVAAAGADCLRINPGNIGSDAKVRE
ECCCCHHHHHHHHHHHHHHHCCEEEEECCCCEEEEEEEECCCCEEEECCCCCCCCHHHHH
VVACAKHYNIPIRIGVNAGSLEKDIQRKYKEPNGAAMLESAMRHIDILERLNFDQYKVSV
HHHHHHHCCCEEEEECCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEE
KASNVFLTMDAYRLISAQIDNPLHLGVTEAGVYRTGSVKSAIALGGLLLDGIGDTIRISL
EECEEEEEEHHHHHHHHHCCCCEEECCCCCCEEECCCHHHHHHHHHHHHCCCCCEEEEEE
AAEPEEEIKIGFDILKSLNIRSNGVNFIACPSCSRQEFDVIRVMTALESRLEDIREPMNL
ECCCHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCE
SVIGCKVNGPGEAKEADIGIVGAAPKSLVYRMGEKSHLIDTDNLVDEIEGMVRAHAEELA
EEEEEEECCCCCCCCCCEEEECCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
KKRENEIIRVR
HHHCCCEEEEC
>Mature Secondary Structure 
STSAPINRRLTKKIYVGDVAIGGDAPISVQSMTNTDTCDVAATVAQIERCVEAGADLMR
CCCCCCHHHHHHEEEEEEEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCEEE
VSTPTMDTVKAFGEIRKLVSVPLIADVHFDHKIALAVAAAGADCLRINPGNIGSDAKVRE
ECCCCHHHHHHHHHHHHHHHCCEEEEECCCCEEEEEEEECCCCEEEECCCCCCCCHHHHH
VVACAKHYNIPIRIGVNAGSLEKDIQRKYKEPNGAAMLESAMRHIDILERLNFDQYKVSV
HHHHHHHCCCEEEEECCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEE
KASNVFLTMDAYRLISAQIDNPLHLGVTEAGVYRTGSVKSAIALGGLLLDGIGDTIRISL
EECEEEEEEHHHHHHHHHCCCCEEECCCCCCEEECCCHHHHHHHHHHHHCCCCCEEEEEE
AAEPEEEIKIGFDILKSLNIRSNGVNFIACPSCSRQEFDVIRVMTALESRLEDIREPMNL
ECCCHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCE
SVIGCKVNGPGEAKEADIGIVGAAPKSLVYRMGEKSHLIDTDNLVDEIEGMVRAHAEELA
EEEEEEECCCCCCCCCCEEEECCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
KKRENEIIRVR
HHHCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA