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The map label for this gene is hisS [H]

Identifier: 93005483

GI number: 93005483

Start: 772386

End: 773690

Strand: Direct

Name: hisS [H]

Synonym: Pcryo_0653

Alternate gene names: 93005483

Gene position: 772386-773690 (Clockwise)

Preceding gene: 93005482

Following gene: 93005484

Centisome position: 25.24

GC content: 45.21

Gene sequence:

>1305_bases
ATGATTAAAGCGATTAAAGGGTTTAATGATATTTTGCCTGCACAGTCTGCAAAATGGCTGTATCTAGAGTCTATATTGGC
TAATGTGCTAGGCAGATACGGTTATGAGCATATTCGCTTGCCTATCGTTGAGCAAACCGATTTGTTTGCTCGCGCGATCG
GCGGTGCTACGGATATCGTTGAAAAAGAGATGTATAGCTTTACCGATAAATCTGATCCACCAACGCCGCTTACCTTGCGT
CCTGAAGGCACGGCAGGGGCAGTACGAGCAGTAAATGAGCACAATCTACTACGCGGTGATACGCCTAAGCTTTGGTATAT
TGGTCCAATGTTCCGCTATGAACGCCCACAAAAGGGTCGCTATCGTCAATTTCATCAAATAGGTGTTGAAAGCTTTGGTA
GTGCCTTACCGGACGCTGATGCCGAGCTGATTGCGATGACTCATTTGATGTGGCAAGAGCTGGGTCTAAAAGATGAGATG
CGTTTAGAGCTAAACAGTCTGGGCGAAATTGATGAGCGTCATGCTTATCGTGAAGCGTTGGTCACCTATCTGACCGATAA
GCAAGAGCAGTTAGATGAGGATAGTAAGCGTCGCCTGACGACTAATCCGCTACGTATTTTAGACAGCAAAGAAGCCAGCA
CACAAGCCCTATTGGTAGACGCGCCTAAGCTTGCTGACTTTTTAGGTGAAGAAAGCGTGGCGCATTTTGAGCAAGTACAA
GCTTATTTAACAGAGCTTGGTATCAACTTTGAAATCAACCCGCATCTGGTGCGTGGTCTTGATTACTATAATAAAACTGT
TTTTGAGTGGGTTACTGACAAGCTTGGCAGTCAAGCAACTGTCTGTGCCGGTGGTCGTTATGATGGCTTGGTTGGACAGC
TAAAATCTGCTGATAACCGTGATGGCAAAGGCGATAATAAACCGGTTAAATCTGAACCTGCAGTTGGTTTTGCCATGGGG
CTTGAGCGTTTATTACTTCTTATGGAAGTTGTCGCGCCTATTGCTGATATGCCTGCTTGTGATGTCTTTGTGGTCGCTCA
TCCTGAGGTTTATAGTGCCGGTATTAGTTATGCGCAAAACCTGCGTTATAGCCGCCCAGATTTGCGCGTCAAAATGGCAA
GTGCCACCAGCTTAAAAGCACAAATGAAAAAAGCAGATAAGTCAGGTGCTGCATTGACCGTTATTATCGCCCAGCAAGAG
CTGGATGATAAAACCATTAGCATCAAAGATATGCAAACTGGCGAACAAAAAACAGCAGCGCAAGATTGGCTGTTGGATAA
AGATAACTTTTCTCGCCATAAATAG

Upstream 100 bases:

>100_bases
AAAAAACGCGAAAACGAGATTATACGTGTTAGATAATAAAGCGTTTTATTAATAAAAATTATAGAGCTGTTTGTTAAAAC
TTAAAGGACATAACCGTACC

Downstream 100 bases:

>100_bases
TAGTGAGCGTAGCATTAGCACTTTAATATAAATTGATCAGGTAAAAGTATATGGCTCTGACCCCTAATTCGCCGAATGCA
GACAACTCAATGCAAGCGTT

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS [H]

Number of amino acids: Translated: 434; Mature: 434

Protein sequence:

>434_residues
MIKAIKGFNDILPAQSAKWLYLESILANVLGRYGYEHIRLPIVEQTDLFARAIGGATDIVEKEMYSFTDKSDPPTPLTLR
PEGTAGAVRAVNEHNLLRGDTPKLWYIGPMFRYERPQKGRYRQFHQIGVESFGSALPDADAELIAMTHLMWQELGLKDEM
RLELNSLGEIDERHAYREALVTYLTDKQEQLDEDSKRRLTTNPLRILDSKEASTQALLVDAPKLADFLGEESVAHFEQVQ
AYLTELGINFEINPHLVRGLDYYNKTVFEWVTDKLGSQATVCAGGRYDGLVGQLKSADNRDGKGDNKPVKSEPAVGFAMG
LERLLLLMEVVAPIADMPACDVFVVAHPEVYSAGISYAQNLRYSRPDLRVKMASATSLKAQMKKADKSGAALTVIIAQQE
LDDKTISIKDMQTGEQKTAAQDWLLDKDNFSRHK

Sequences:

>Translated_434_residues
MIKAIKGFNDILPAQSAKWLYLESILANVLGRYGYEHIRLPIVEQTDLFARAIGGATDIVEKEMYSFTDKSDPPTPLTLR
PEGTAGAVRAVNEHNLLRGDTPKLWYIGPMFRYERPQKGRYRQFHQIGVESFGSALPDADAELIAMTHLMWQELGLKDEM
RLELNSLGEIDERHAYREALVTYLTDKQEQLDEDSKRRLTTNPLRILDSKEASTQALLVDAPKLADFLGEESVAHFEQVQ
AYLTELGINFEINPHLVRGLDYYNKTVFEWVTDKLGSQATVCAGGRYDGLVGQLKSADNRDGKGDNKPVKSEPAVGFAMG
LERLLLLMEVVAPIADMPACDVFVVAHPEVYSAGISYAQNLRYSRPDLRVKMASATSLKAQMKKADKSGAALTVIIAQQE
LDDKTISIKDMQTGEQKTAAQDWLLDKDNFSRHK
>Mature_434_residues
MIKAIKGFNDILPAQSAKWLYLESILANVLGRYGYEHIRLPIVEQTDLFARAIGGATDIVEKEMYSFTDKSDPPTPLTLR
PEGTAGAVRAVNEHNLLRGDTPKLWYIGPMFRYERPQKGRYRQFHQIGVESFGSALPDADAELIAMTHLMWQELGLKDEM
RLELNSLGEIDERHAYREALVTYLTDKQEQLDEDSKRRLTTNPLRILDSKEASTQALLVDAPKLADFLGEESVAHFEQVQ
AYLTELGINFEINPHLVRGLDYYNKTVFEWVTDKLGSQATVCAGGRYDGLVGQLKSADNRDGKGDNKPVKSEPAVGFAMG
LERLLLLMEVVAPIADMPACDVFVVAHPEVYSAGISYAQNLRYSRPDLRVKMASATSLKAQMKKADKSGAALTVIIAQQE
LDDKTISIKDMQTGEQKTAAQDWLLDKDNFSRHK

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1788861, Length=425, Percent_Identity=53.4117647058823, Blast_Score=439, Evalue=1e-124,
Organism=Caenorhabditis elegans, GI71993693, Length=486, Percent_Identity=26.3374485596708, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI71993686, Length=486, Percent_Identity=26.3374485596708, Blast_Score=89, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.21 [H]

Molecular weight: Translated: 48582; Mature: 48582

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKAIKGFNDILPAQSAKWLYLESILANVLGRYGYEHIRLPIVEQTDLFARAIGGATDIV
CCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHCCHHHHH
EKEMYSFTDKSDPPTPLTLRPEGTAGAVRAVNEHNLLRGDTPKLWYIGPMFRYERPQKGR
HHHHHHHCCCCCCCCCEEECCCCCCHHHEECCCCCCCCCCCCCEEEECCHHHCCCCCCCH
YRQFHQIGVESFGSALPDADAELIAMTHLMWQELGLKDEMRLELNSLGEIDERHAYREAL
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHH
VTYLTDKQEQLDEDSKRRLTTNPLRILDSKEASTQALLVDAPKLADFLGEESVAHFEQVQ
HHHHCCCHHHHCCHHHHCCCCCCHHEECCCCCCCCEEEEECHHHHHHHCCHHHHHHHHHH
AYLTELGINFEINPHLVRGLDYYNKTVFEWVTDKLGSQATVCAGGRYDGLVGQLKSADNR
HHHHHHCCCEEECHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHCCCCC
DGKGDNKPVKSEPAVGFAMGLERLLLLMEVVAPIADMPACDVFVVAHPEVYSAGISYAQN
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHH
LRYSRPDLRVKMASATSLKAQMKKADKSGAALTVIIAQQELDDKTISIKDMQTGEQKTAA
CCCCCCCCEEEECCHHHHHHHHHHCCCCCCEEEEEEECCCCCCCEEEEECCCCCCHHHHH
QDWLLDKDNFSRHK
HHHCCCCCCCCCCC
>Mature Secondary Structure
MIKAIKGFNDILPAQSAKWLYLESILANVLGRYGYEHIRLPIVEQTDLFARAIGGATDIV
CCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHCCHHHHH
EKEMYSFTDKSDPPTPLTLRPEGTAGAVRAVNEHNLLRGDTPKLWYIGPMFRYERPQKGR
HHHHHHHCCCCCCCCCEEECCCCCCHHHEECCCCCCCCCCCCCEEEECCHHHCCCCCCCH
YRQFHQIGVESFGSALPDADAELIAMTHLMWQELGLKDEMRLELNSLGEIDERHAYREAL
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHH
VTYLTDKQEQLDEDSKRRLTTNPLRILDSKEASTQALLVDAPKLADFLGEESVAHFEQVQ
HHHHCCCHHHHCCHHHHCCCCCCHHEECCCCCCCCEEEEECHHHHHHHCCHHHHHHHHHH
AYLTELGINFEINPHLVRGLDYYNKTVFEWVTDKLGSQATVCAGGRYDGLVGQLKSADNR
HHHHHHCCCEEECHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHCCCCC
DGKGDNKPVKSEPAVGFAMGLERLLLLMEVVAPIADMPACDVFVVAHPEVYSAGISYAQN
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHH
LRYSRPDLRVKMASATSLKAQMKKADKSGAALTVIIAQQELDDKTISIKDMQTGEQKTAA
CCCCCCCCEEEECCHHHHHHHHHHCCCCCCEEEEEEECCCCCCCEEEEECCCCCCHHHHH
QDWLLDKDNFSRHK
HHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA