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The map label for this gene is mscS [H]

Identifier: 93005419

GI number: 93005419

Start: 703337

End: 704212

Strand: Direct

Name: mscS [H]

Synonym: Pcryo_0589

Alternate gene names: 93005419

Gene position: 703337-704212 (Clockwise)

Preceding gene: 93005418

Following gene: 93005422

Centisome position: 22.99

GC content: 42.69

Gene sequence:

>876_bases
ATGGAATTTTTAGGTTTTACCCTTGATGTTGCCACTTTAACCAATAACGCTTTGAGTATTGCTGCTAAAGTGGCATTAGC
GCTACTAATATTTATGATAGGGCGCTGGCTTGCCAAAAAAGCCGTCACATTTGCTCACGGTATGATGTTGCGTAGTCGTT
TAGATGAGACAGTCGCTAGTTTTTTAGGTCGCTTAATTTACGGTGTGCTATTGGTTGTGGTCGTTTTGGCTGCCTTGAGT
AAAGTTGGTGTGCAGACCACTTCAGTCGTGGCTATCTTGGGTGGTGCAGCGGTTGCGATTGGTTTATCACTGAAAGATCA
GCTGTCTAATTTTGCAGCGGGCATTATGATTGTCACCTTTCGCCCCTTTGTGCGCGGTGATTATGTGCAAATAAGCAGCT
ATACGGGCACTGTGACAGAGATTACCTTGGTCAATACGCATCTAACAACGATTAACAATCATGATGTCATCATTCCTAAT
AGCGATATCACCACTTCAGCGGTTGTTAATTACACCGCATTGCCCAATCGCCGCGTCGATATCACCGTTGGTATTGGTTA
TGATGCTGATATCAAAACTGCCAAAGATGTTATGCTAAATTTGGCAATCAGTAATCCGAAGGCGTTTACTGATCCTGCAC
CGGTTATTCGCGTCACTAACTTGGGCGATAACTCCGTTGATTTAACTTTAAACGTTTGGACGACTAATGACGACTGGTGG
TCAATGCAGTGCGATCTATTGGAACAGTTTAAATATGCACTGGATGATAATAAAATTGATATTCCGTTCCCGCAGCGTAA
TGTCCATGTGAAAGGACTTGACCAGATGATTAATCAGATGGATCAGGCACAAAAACTACTAACGAAAAAAGATTAG

Upstream 100 bases:

>100_bases
AGAAGTGACGTACTAGAAGCGTTAAAAGCAGTCCTGCATTTTCAACGTATTAACTAAAACAGTAAATAGATAACACCCAG
ATATAGAGTAAAGAGTTTAT

Downstream 100 bases:

>100_bases
TTATAAGGGTTTGGAATATTGAGCCCTGCCAAAATTTCAATCTCAAAGCGCTCCATCTCATCTTGCTCGGCTTGCCCGAG
CTCATGATCGAAACCGAGTA

Product: mechanosensitive ion channel MscS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 291; Mature: 291

Protein sequence:

>291_residues
MEFLGFTLDVATLTNNALSIAAKVALALLIFMIGRWLAKKAVTFAHGMMLRSRLDETVASFLGRLIYGVLLVVVVLAALS
KVGVQTTSVVAILGGAAVAIGLSLKDQLSNFAAGIMIVTFRPFVRGDYVQISSYTGTVTEITLVNTHLTTINNHDVIIPN
SDITTSAVVNYTALPNRRVDITVGIGYDADIKTAKDVMLNLAISNPKAFTDPAPVIRVTNLGDNSVDLTLNVWTTNDDWW
SMQCDLLEQFKYALDDNKIDIPFPQRNVHVKGLDQMINQMDQAQKLLTKKD

Sequences:

>Translated_291_residues
MEFLGFTLDVATLTNNALSIAAKVALALLIFMIGRWLAKKAVTFAHGMMLRSRLDETVASFLGRLIYGVLLVVVVLAALS
KVGVQTTSVVAILGGAAVAIGLSLKDQLSNFAAGIMIVTFRPFVRGDYVQISSYTGTVTEITLVNTHLTTINNHDVIIPN
SDITTSAVVNYTALPNRRVDITVGIGYDADIKTAKDVMLNLAISNPKAFTDPAPVIRVTNLGDNSVDLTLNVWTTNDDWW
SMQCDLLEQFKYALDDNKIDIPFPQRNVHVKGLDQMINQMDQAQKLLTKKD
>Mature_291_residues
MEFLGFTLDVATLTNNALSIAAKVALALLIFMIGRWLAKKAVTFAHGMMLRSRLDETVASFLGRLIYGVLLVVVVLAALS
KVGVQTTSVVAILGGAAVAIGLSLKDQLSNFAAGIMIVTFRPFVRGDYVQISSYTGTVTEITLVNTHLTTINNHDVIIPN
SDITTSAVVNYTALPNRRVDITVGIGYDADIKTAKDVMLNLAISNPKAFTDPAPVIRVTNLGDNSVDLTLNVWTTNDDWW
SMQCDLLEQFKYALDDNKIDIPFPQRNVHVKGLDQMINQMDQAQKLLTKKD

Specific function: Mechanosensitive channel that participates in the regulation of osmotic pressure changes within the cell. Forms an ion channel of 1.0 nanosiemens conductance with a slight preference for anions. The channel is sensitive to voltage [H]

COG id: COG0668

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1789291, Length=258, Percent_Identity=34.1085271317829, Blast_Score=179, Evalue=1e-46,
Organism=Escherichia coli, GI1786670, Length=204, Percent_Identity=26.9607843137255, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1787591, Length=223, Percent_Identity=26.0089686098655, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI2367355, Length=258, Percent_Identity=23.2558139534884, Blast_Score=71, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014
- InterPro:   IPR008910 [H]

Pfam domain/function: PF00924 MS_channel; PF05552 TM_helix [H]

EC number: NA

Molecular weight: Translated: 31800; Mature: 31800

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFLGFTLDVATLTNNALSIAAKVALALLIFMIGRWLAKKAVTFAHGMMLRSRLDETVAS
CCCCCEEEEEHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FLGRLIYGVLLVVVVLAALSKVGVQTTSVVAILGGAAVAIGLSLKDQLSNFAAGIMIVTF
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHEEEECCHHHHHHHHHCCEEEEEE
RPFVRGDYVQISSYTGTVTEITLVNTHLTTINNHDVIIPNSDITTSAVVNYTALPNRRVD
CCCCCCCEEEEECCCCCEEEEEEEEEEEEEECCCEEEECCCCCCHHHEEEEEECCCCEEE
ITVGIGYDADIKTAKDVMLNLAISNPKAFTDPAPVIRVTNLGDNSVDLTLNVWTTNDDWW
EEEECCCCCCCHHHHHHEEEEEECCCCCCCCCCCEEEEEECCCCCEEEEEEEEECCCCCE
SMQCDLLEQFKYALDDNKIDIPFPQRNVHVKGLDQMINQMDQAQKLLTKKD
EEHHHHHHHHHHHHCCCEEECCCCCCCEEECCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEFLGFTLDVATLTNNALSIAAKVALALLIFMIGRWLAKKAVTFAHGMMLRSRLDETVAS
CCCCCEEEEEHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FLGRLIYGVLLVVVVLAALSKVGVQTTSVVAILGGAAVAIGLSLKDQLSNFAAGIMIVTF
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHEEEECCHHHHHHHHHCCEEEEEE
RPFVRGDYVQISSYTGTVTEITLVNTHLTTINNHDVIIPNSDITTSAVVNYTALPNRRVD
CCCCCCCEEEEECCCCCEEEEEEEEEEEEEECCCEEEECCCCCCHHHEEEEEECCCCEEE
ITVGIGYDADIKTAKDVMLNLAISNPKAFTDPAPVIRVTNLGDNSVDLTLNVWTTNDDWW
EEEECCCCCCCHHHHHHEEEEEECCCCCCCCCCCEEEEEECCCCCEEEEEEEEECCCCCE
SMQCDLLEQFKYALDDNKIDIPFPQRNVHVKGLDQMINQMDQAQKLLTKKD
EEHHHHHHHHHHHHCCCEEECCCCCCCEEECCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12542086 [H]