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The map label for this gene is ubiH [H]

Identifier: 93005422

GI number: 93005422

Start: 706677

End: 707981

Strand: Direct

Name: ubiH [H]

Synonym: Pcryo_0592

Alternate gene names: 93005422

Gene position: 706677-707981 (Clockwise)

Preceding gene: 93005419

Following gene: 93005423

Centisome position: 23.09

GC content: 46.59

Gene sequence:

>1305_bases
ATGATGCAAACTACTGAATCAAATTTATCAAAACTTAATAATATTAAACACACTGCGATGACTGAGCAGCAGGTACTTAT
CGCTGGTGGCGGTCATGTCGGTTTGTCGTTTGCGTTGCTATTGGCAAACCATGGTATCGCCAGTACATTGCTCGAGAAGA
ACAGCTATCCGACCATCAGTCCAAATGATGATAGCAATCGCAGCCACTATTTAGACAGCCGTAATACGGCATTGTCACGG
CGTACGGTACAGATTTATCAAGAGATCGGGCTGTGGGATGAACTACAAAGCCATGCCTGCCGTATTGATGCCGTACAGAT
TAGTGAACAAGGTAGCTTTGGACGCGCGCAACTCAATAAAAAGGATGAACAAGTTGAGTCGTTTGGACAAGTGATGGAAA
ATGCGTGGCTTGGGCGTAAGTTACTCCTTGCTGCCCAGCAAGAGCCACTGATTACGCTCATTGATAATGCAAATGTAATA
GCCGTTGACCAGAAAAGCGATAGCGTAACCCTAAGTTTTAGCTATTATGGTGAAGAAGAGCATGAGCAGCAATTGCAGGG
TAGTGTGTTGGTCGCCTGTGATGGTCGCGACTCTACCGTGCGTCAGTTATTAGATATCGGTACGACTACTTATGACTATA
AGCAAACGGCTATCGTCGGCGTGGTAGAAACGGACAAACCACATGAGCATAATGCGATTGAGCGTTTTAGTCCTGCCGGA
CCACTGGCAGTGTTGCCATTAACGGATCCAGAAGGCGACGGTAATGACGAATATCAGACTGGCTATAGACGTTCAGTCGT
TTGGGTCTGCCGAACCGGAGAAGAGACGCAATACTTAGAGGATGACGCGCATTTCTTAGCGACTTTGCAGCAAGCTTTTG
GTCAGCGTGCAGGCACGTTTAAAACTGCTGGTCGCCGTGGTGCATATCCATTGACACAAGTGCTGGCAGATAAACAAGTG
GATGGTCGCTGCGTTATCATGGGTAATGCAGCGCACACCCTGCATCCAGTAGCAGGACAAGGGTTTAACCTATGTATGCG
TGATGCTCATGTACTGGCACAAATGATGAGTGCGCAAGTACTAAAAGGCGAAGACATCGGTAATCCTACATTACTAAAGC
GTTATGAGAAAGCCCGTCAAACTGACCAAAAACGCGTTATTCGTTTTTGTGATGCGGTGGTACATGGCTTTACGCATCCA
AATCCAGCGGTGAAGCTGGCGCGTAATGTCGCCTTGGTTGCCTTCGATAAATTACCGAATATTAAACCATTAATTGCCAA
TTATGCGATGGGTCTAAAGTCTTAG

Upstream 100 bases:

>100_bases
AATATGTTCAAAGAACAATAGGTTTTTCAATAAAATAAAATTTTGAGTGAATAATAATCACGTATAAATGCGTGCTAAAT
CGACAATGATAGGAATAATA

Downstream 100 bases:

>100_bases
TATTGATAAGCCATTAAAAAAATTCAAAAATCTAACTGCTGCTAATTATTAAAAATATCAGCGGATAACTCAAGTAGCGA
AACGAAGGATATTTTATGAA

Product: UbiH/UbiF/VisC/COQ6 family ubiquinone biosynthesis hydroxylase

Products: H2O; 2-octaprenyl-6-methoxy-1,4-benzoquinone [C]

Alternate protein names: NA

Number of amino acids: Translated: 434; Mature: 434

Protein sequence:

>434_residues
MMQTTESNLSKLNNIKHTAMTEQQVLIAGGGHVGLSFALLLANHGIASTLLEKNSYPTISPNDDSNRSHYLDSRNTALSR
RTVQIYQEIGLWDELQSHACRIDAVQISEQGSFGRAQLNKKDEQVESFGQVMENAWLGRKLLLAAQQEPLITLIDNANVI
AVDQKSDSVTLSFSYYGEEEHEQQLQGSVLVACDGRDSTVRQLLDIGTTTYDYKQTAIVGVVETDKPHEHNAIERFSPAG
PLAVLPLTDPEGDGNDEYQTGYRRSVVWVCRTGEETQYLEDDAHFLATLQQAFGQRAGTFKTAGRRGAYPLTQVLADKQV
DGRCVIMGNAAHTLHPVAGQGFNLCMRDAHVLAQMMSAQVLKGEDIGNPTLLKRYEKARQTDQKRVIRFCDAVVHGFTHP
NPAVKLARNVALVAFDKLPNIKPLIANYAMGLKS

Sequences:

>Translated_434_residues
MMQTTESNLSKLNNIKHTAMTEQQVLIAGGGHVGLSFALLLANHGIASTLLEKNSYPTISPNDDSNRSHYLDSRNTALSR
RTVQIYQEIGLWDELQSHACRIDAVQISEQGSFGRAQLNKKDEQVESFGQVMENAWLGRKLLLAAQQEPLITLIDNANVI
AVDQKSDSVTLSFSYYGEEEHEQQLQGSVLVACDGRDSTVRQLLDIGTTTYDYKQTAIVGVVETDKPHEHNAIERFSPAG
PLAVLPLTDPEGDGNDEYQTGYRRSVVWVCRTGEETQYLEDDAHFLATLQQAFGQRAGTFKTAGRRGAYPLTQVLADKQV
DGRCVIMGNAAHTLHPVAGQGFNLCMRDAHVLAQMMSAQVLKGEDIGNPTLLKRYEKARQTDQKRVIRFCDAVVHGFTHP
NPAVKLARNVALVAFDKLPNIKPLIANYAMGLKS
>Mature_434_residues
MMQTTESNLSKLNNIKHTAMTEQQVLIAGGGHVGLSFALLLANHGIASTLLEKNSYPTISPNDDSNRSHYLDSRNTALSR
RTVQIYQEIGLWDELQSHACRIDAVQISEQGSFGRAQLNKKDEQVESFGQVMENAWLGRKLLLAAQQEPLITLIDNANVI
AVDQKSDSVTLSFSYYGEEEHEQQLQGSVLVACDGRDSTVRQLLDIGTTTYDYKQTAIVGVVETDKPHEHNAIERFSPAG
PLAVLPLTDPEGDGNDEYQTGYRRSVVWVCRTGEETQYLEDDAHFLATLQQAFGQRAGTFKTAGRRGAYPLTQVLADKQV
DGRCVIMGNAAHTLHPVAGQGFNLCMRDAHVLAQMMSAQVLKGEDIGNPTLLKRYEKARQTDQKRVIRFCDAVVHGFTHP
NPAVKLARNVALVAFDKLPNIKPLIANYAMGLKS

Specific function: Oxygenase that introduces the hydroxyl group at carbon four of 2-octaprenyl-6-methoxyphenol resulting in the formation of 2-octaprenyl-6-methoxy-1,4-benzoquinone [H]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ubiH/COQ6 family [H]

Homologues:

Organism=Homo sapiens, GI32967307, Length=271, Percent_Identity=28.0442804428044, Blast_Score=93, Evalue=4e-19,
Organism=Homo sapiens, GI32967305, Length=280, Percent_Identity=26.4285714285714, Blast_Score=93, Evalue=4e-19,
Organism=Escherichia coli, GI1789274, Length=413, Percent_Identity=33.8983050847458, Blast_Score=206, Evalue=2e-54,
Organism=Escherichia coli, GI1789273, Length=416, Percent_Identity=29.5673076923077, Blast_Score=169, Evalue=4e-43,
Organism=Escherichia coli, GI1786883, Length=417, Percent_Identity=28.537170263789, Blast_Score=149, Evalue=4e-37,
Organism=Caenorhabditis elegans, GI25146631, Length=449, Percent_Identity=26.0579064587973, Blast_Score=122, Evalue=3e-28,
Organism=Drosophila melanogaster, GI19920760, Length=402, Percent_Identity=23.8805970149254, Blast_Score=97, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042
- InterPro:   IPR018168
- InterPro:   IPR010971
- InterPro:   IPR011295 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.- [C]

Molecular weight: Translated: 47920; Mature: 47920

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMQTTESNLSKLNNIKHTAMTEQQVLIAGGGHVGLSFALLLANHGIASTLLEKNSYPTIS
CCCCCHHHHHHHHCCHHHHCCCCEEEEECCCHHHHHHHHHHHCCCHHHHHHHCCCCCCCC
PNDDSNRSHYLDSRNTALSRRTVQIYQEIGLWDELQSHACRIDAVQISEQGSFGRAQLNK
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHCCEEEEEEEEECCCCCCHHHCCC
KDEQVESFGQVMENAWLGRKLLLAAQQEPLITLIDNANVIAVDQKSDSVTLSFSYYGEEE
HHHHHHHHHHHHHHHHCCHHHEEECCCCCEEEEECCCCEEEEECCCCEEEEEEEECCCHH
HEQQLQGSVLVACDGRDSTVRQLLDIGTTTYDYKQTAIVGVVETDKPHEHNAIERFSPAG
HHHHHCCCEEEEECCCCHHHHHHHHCCCCCCCCCCEEEEEEEECCCCCCCCHHHHCCCCC
PLAVLPLTDPEGDGNDEYQTGYRRSVVWVCRTGEETQYLEDDAHFLATLQQAFGQRAGTF
CEEEEECCCCCCCCCCHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCC
KTAGRRGAYPLTQVLADKQVDGRCVIMGNAAHTLHPVAGQGFNLCMRDAHVLAQMMSAQV
CCCCCCCCCHHHHHHHCCCCCCEEEEECCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHH
LKGEDIGNPTLLKRYEKARQTDQKRVIRFCDAVVHGFTHPNPAVKLARNVALVAFDKLPN
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCEEEEEECCCCC
IKPLIANYAMGLKS
CHHHHHHHHHCCCC
>Mature Secondary Structure
MMQTTESNLSKLNNIKHTAMTEQQVLIAGGGHVGLSFALLLANHGIASTLLEKNSYPTIS
CCCCCHHHHHHHHCCHHHHCCCCEEEEECCCHHHHHHHHHHHCCCHHHHHHHCCCCCCCC
PNDDSNRSHYLDSRNTALSRRTVQIYQEIGLWDELQSHACRIDAVQISEQGSFGRAQLNK
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHCCEEEEEEEEECCCCCCHHHCCC
KDEQVESFGQVMENAWLGRKLLLAAQQEPLITLIDNANVIAVDQKSDSVTLSFSYYGEEE
HHHHHHHHHHHHHHHHCCHHHEEECCCCCEEEEECCCCEEEEECCCCEEEEEEEECCCHH
HEQQLQGSVLVACDGRDSTVRQLLDIGTTTYDYKQTAIVGVVETDKPHEHNAIERFSPAG
HHHHHCCCEEEEECCCCHHHHHHHHCCCCCCCCCCEEEEEEEECCCCCCCCHHHHCCCCC
PLAVLPLTDPEGDGNDEYQTGYRRSVVWVCRTGEETQYLEDDAHFLATLQQAFGQRAGTF
CEEEEECCCCCCCCCCHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCC
KTAGRRGAYPLTQVLADKQVDGRCVIMGNAAHTLHPVAGQGFNLCMRDAHVLAQMMSAQV
CCCCCCCCCHHHHHHHCCCCCCEEEEECCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHH
LKGEDIGNPTLLKRYEKARQTDQKRVIRFCDAVVHGFTHPNPAVKLARNVALVAFDKLPN
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCEEEEEECCCCC
IKPLIANYAMGLKS
CHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-octaprenyl-6-methoxyphenol; O2 [C]

Specific reaction: 2-octaprenyl-6-methoxyphenol + O2 = H2O + 2-octaprenyl-6-methoxy-1,4-benzoquinone [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1339425; 9278503 [H]