Click here to switch to the map view.

The map label for this gene is smpB [H]

Identifier: 93005189

GI number: 93005189

Start: 437430

End: 437906

Strand: Reverse

Name: smpB [H]

Synonym: Pcryo_0358

Alternate gene names: 93005189

Gene position: 437906-437430 (Counterclockwise)

Preceding gene: 93005201

Following gene: 93005187

Centisome position: 14.31

GC content: 42.56

Gene sequence:

>477_bases
ATGTCAAAAAAATCAAAAAAACCGGAAAATCAAATTTGTGCCAATAAAAAGGCTCGTCATGAGTACTTTATTGAAGAGAC
ATTTGAGGCAGGTCTGTCGTTACAAGGTTGGGAAGTTAAAGCCATTCGCGCGGGCAAAATGACCATTACGGAAGCTTATA
TTATTTTTCGTAATAATGAGGCATTCCTATTTGGCGCGCATATTCAGCCGCTGCTATCCAGCTCGACGCATGTCAGCCCT
GACAGTATCCGTACCCGTAAGCTGCTGCTTAATCGCCGCGAAATTGAAAAGCTAATGGGTGCAGTCAACCAAAAAGGCTA
TGCCTGCGTGCCATTATCCTGCTATTGGAAAAACTCGCTGGTAAAATGTCAAATTGGCTTGGCACTGGGTAAAAAACAGC
ATGACAAACGTAAAACGCTTAAAGACCGTGATTGGGAACGTGATAAGCAGCGTGGTTTTAAGAAGGATTTAGATTAA

Upstream 100 bases:

>100_bases
ATTTATAAAAAATAACCCGCATTTAAGGGTTAGGATGAGGAATTAGTAAGACTAGAAATATAGGGACAAGATATATTTAA
GCAGTCCTTATGGAGACATT

Downstream 100 bases:

>100_bases
AGCCCTCTTTCAAAAGATTGGGGTTAATCTTTTGAAAACGGACGATTCAGCATAATGTTGAATCGTCCGTTTTTATTTAT
TCGATTACTCTTATTGATTA

Product: SsrA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 158; Mature: 157

Protein sequence:

>158_residues
MSKKSKKPENQICANKKARHEYFIEETFEAGLSLQGWEVKAIRAGKMTITEAYIIFRNNEAFLFGAHIQPLLSSSTHVSP
DSIRTRKLLLNRREIEKLMGAVNQKGYACVPLSCYWKNSLVKCQIGLALGKKQHDKRKTLKDRDWERDKQRGFKKDLD

Sequences:

>Translated_158_residues
MSKKSKKPENQICANKKARHEYFIEETFEAGLSLQGWEVKAIRAGKMTITEAYIIFRNNEAFLFGAHIQPLLSSSTHVSP
DSIRTRKLLLNRREIEKLMGAVNQKGYACVPLSCYWKNSLVKCQIGLALGKKQHDKRKTLKDRDWERDKQRGFKKDLD
>Mature_157_residues
SKKSKKPENQICANKKARHEYFIEETFEAGLSLQGWEVKAIRAGKMTITEAYIIFRNNEAFLFGAHIQPLLSSSTHVSPD
SIRTRKLLLNRREIEKLMGAVNQKGYACVPLSCYWKNSLVKCQIGLALGKKQHDKRKTLKDRDWERDKQRGFKKDLD

Specific function: Binds specifically to the ssrA RNA (tmRNA) and is required for stable association of ssrA with ribosomes [H]

COG id: COG0691

COG function: function code O; tmRNA-binding protein

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smpB family [H]

Homologues:

Organism=Escherichia coli, GI1788973, Length=153, Percent_Identity=59.4771241830065, Blast_Score=191, Evalue=1e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000037
- InterPro:   IPR020081
- ProDom:   PD004488 [H]

Pfam domain/function: PF01668 SmpB [H]

EC number: NA

Molecular weight: Translated: 18284; Mature: 18153

Theoretical pI: Translated: 10.43; Mature: 10.43

Prosite motif: PS01317 SSRP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKKSKKPENQICANKKARHEYFIEETFEAGLSLQGWEVKAIRAGKMTITEAYIIFRNNE
CCCCCCCCCHHHHCCCHHHHHHHHHHHHHCCCCCCCCEEEEEECCCEEEEEEEEEEECCC
AFLFGAHIQPLLSSSTHVSPDSIRTRKLLLNRREIEKLMGAVNQKGYACVPLSCYWKNSL
EEEECCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCE
VKCQIGLALGKKQHDKRKTLKDRDWERDKQRGFKKDLD
EEEEEEHHHCCCHHHHHHHHCCCCCCHHHHCCCCCCCC
>Mature Secondary Structure 
SKKSKKPENQICANKKARHEYFIEETFEAGLSLQGWEVKAIRAGKMTITEAYIIFRNNE
CCCCCCCCHHHHCCCHHHHHHHHHHHHHCCCCCCCCEEEEEECCCEEEEEEEEEEECCC
AFLFGAHIQPLLSSSTHVSPDSIRTRKLLLNRREIEKLMGAVNQKGYACVPLSCYWKNSL
EEEECCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCE
VKCQIGLALGKKQHDKRKTLKDRDWERDKQRGFKKDLD
EEEEEEHHHCCCHHHHHHHHCCCCCCHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA