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The map label for this gene is amiD [H]

Identifier: 93005043

GI number: 93005043

Start: 254331

End: 255215

Strand: Reverse

Name: amiD [H]

Synonym: Pcryo_0212

Alternate gene names: 93005043

Gene position: 255215-254331 (Counterclockwise)

Preceding gene: 93005044

Following gene: 93005042

Centisome position: 8.34

GC content: 42.15

Gene sequence:

>885_bases
ATGGCAAAAAATATCTTTTTAGCATTAGCATGTGCCTTAACGCTGCCTTTAGTTGGCTGCGTGACCACTAATAACACCCA
AATAGCGAATACTGAACCCTTCGTTATTGATAGCGAAACCTATCAAGCCACTGGCAAAAGCGAGCGGATTAAATCCATAG
TCCTGCACTACACCGTCTCAGATAATGCCAGATCTATAAAAACACTGACCACTGGAAATGTGAGCGCCCACTATCTGATC
TTGGATAATAACGATAATAAAATTTATAACTTAGTGCCTGAAAGCGAACGTGCTTGGCATGCTGGCGCGGGCGGCTTTGC
TGGTAGAACGATACTCAACGATACCTCTATCGGCATCGAGATTGTCAATGCAGGCATTAAGCCTAAATATCGAGACGCCT
TAAAAAACGGCGCGCTCGACTATCATCCTTATGAGCATTATGTTGAATTTAATGAATTACAGATTAAGAAAGTGGCGCAG
CTGGTGCAAGACTTGGCAAAAAGATATAATATCTCAGCAAAGAATATAATCGGTCATGCGGATATGGCACCCTCGCGTAA
AATCGATTCCGGTGCAAAATTTCCTTGGCAGCGACTGTATTTCGATTACGGCATTGGCGCTTGGTATGATGAATTTGATA
AGCAATACTTTATGAATCAAGACGAGTTTGCAGCGGTAACGATACCTGAGATTAAGCAAGCGTTTCGCGATTACGGTTAT
CAAATCAATGACACAGACGCATGGGATAAAGCCAGTCGCAATGTCGTGTATGCTTTTCAATTGCATTTTAGACCACAGCA
GCCAACGGGCGAGCTAGATTTAGAAACTTATGCAATATTAAAGGCACTCAATAAGAAGTATGCTGGTCGTGATGACTTTT
ATTAA

Upstream 100 bases:

>100_bases
ATTTGCCGCAGAAGTTATTGAACCTTGGCTTGCGATTGAGTGTTTGGAAGAGAGTTGGAAGAAGGTTTTGGCAAAGAAGA
TTAAAAAGGGTTAGCATTTC

Downstream 100 bases:

>100_bases
TCGATAAAAAATATTAGGAGACATAAGCCATGAGCAACTCTCAATACAACGAGCAAAATGAGCAAGACGAGCTACAGAAG
TATCTAGAAGAAAAAGCCAA

Product: negative regulator of AmpC, AmpD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 294; Mature: 293

Protein sequence:

>294_residues
MAKNIFLALACALTLPLVGCVTTNNTQIANTEPFVIDSETYQATGKSERIKSIVLHYTVSDNARSIKTLTTGNVSAHYLI
LDNNDNKIYNLVPESERAWHAGAGGFAGRTILNDTSIGIEIVNAGIKPKYRDALKNGALDYHPYEHYVEFNELQIKKVAQ
LVQDLAKRYNISAKNIIGHADMAPSRKIDSGAKFPWQRLYFDYGIGAWYDEFDKQYFMNQDEFAAVTIPEIKQAFRDYGY
QINDTDAWDKASRNVVYAFQLHFRPQQPTGELDLETYAILKALNKKYAGRDDFY

Sequences:

>Translated_294_residues
MAKNIFLALACALTLPLVGCVTTNNTQIANTEPFVIDSETYQATGKSERIKSIVLHYTVSDNARSIKTLTTGNVSAHYLI
LDNNDNKIYNLVPESERAWHAGAGGFAGRTILNDTSIGIEIVNAGIKPKYRDALKNGALDYHPYEHYVEFNELQIKKVAQ
LVQDLAKRYNISAKNIIGHADMAPSRKIDSGAKFPWQRLYFDYGIGAWYDEFDKQYFMNQDEFAAVTIPEIKQAFRDYGY
QINDTDAWDKASRNVVYAFQLHFRPQQPTGELDLETYAILKALNKKYAGRDDFY
>Mature_293_residues
AKNIFLALACALTLPLVGCVTTNNTQIANTEPFVIDSETYQATGKSERIKSIVLHYTVSDNARSIKTLTTGNVSAHYLIL
DNNDNKIYNLVPESERAWHAGAGGFAGRTILNDTSIGIEIVNAGIKPKYRDALKNGALDYHPYEHYVEFNELQIKKVAQL
VQDLAKRYNISAKNIIGHADMAPSRKIDSGAKFPWQRLYFDYGIGAWYDEFDKQYFMNQDEFAAVTIPEIKQAFRDYGYQ
INDTDAWDKASRNVVYAFQLHFRPQQPTGELDLETYAILKALNKKYAGRDDFY

Specific function: Unknown

COG id: COG3023

COG function: function code V; Negative regulator of beta-lactamase expression

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1787092, Length=279, Percent_Identity=38.3512544802867, Blast_Score=171, Evalue=5e-44,
Organism=Escherichia coli, GI1786300, Length=127, Percent_Identity=36.2204724409449, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502
- InterPro:   IPR002477 [H]

Pfam domain/function: PF01510 Amidase_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 33335; Mature: 33204

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKNIFLALACALTLPLVGCVTTNNTQIANTEPFVIDSETYQATGKSERIKSIVLHYTVS
CCCHHHHHHHHHHHHHHHEEEECCCCEECCCCCEEEECCCCCCCCCHHHEEEEEEEEEEC
DNARSIKTLTTGNVSAHYLILDNNDNKIYNLVPESERAWHAGAGGFAGRTILNDTSIGIE
CCCCEEEEEEECCCEEEEEEEECCCCEEEEECCCCCCCEECCCCCCCCCEEECCCCCCEE
IVNAGIKPKYRDALKNGALDYHPYEHYVEFNELQIKKVAQLVQDLAKRYNISAKNIIGHA
EEECCCCCHHHHHHHCCCCCCCCHHHHEEHHHHHHHHHHHHHHHHHHHHCCCHHHEECCC
DMAPSRKIDSGAKFPWQRLYFDYGIGAWYDEFDKQYFMNQDEFAAVTIPEIKQAFRDYGY
CCCCCCCCCCCCCCCHHHHEEECCCCHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHCCC
QINDTDAWDKASRNVVYAFQLHFRPQQPTGELDLETYAILKALNKKYAGRDDFY
EECCCCCCHHCCCCEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
AKNIFLALACALTLPLVGCVTTNNTQIANTEPFVIDSETYQATGKSERIKSIVLHYTVS
CCHHHHHHHHHHHHHHHEEEECCCCEECCCCCEEEECCCCCCCCCHHHEEEEEEEEEEC
DNARSIKTLTTGNVSAHYLILDNNDNKIYNLVPESERAWHAGAGGFAGRTILNDTSIGIE
CCCCEEEEEEECCCEEEEEEEECCCCEEEEECCCCCCCEECCCCCCCCCEEECCCCCCEE
IVNAGIKPKYRDALKNGALDYHPYEHYVEFNELQIKKVAQLVQDLAKRYNISAKNIIGHA
EEECCCCCHHHHHHHCCCCCCCCHHHHEEHHHHHHHHHHHHHHHHHHHHCCCHHHEECCC
DMAPSRKIDSGAKFPWQRLYFDYGIGAWYDEFDKQYFMNQDEFAAVTIPEIKQAFRDYGY
CCCCCCCCCCCCCCCHHHHEEECCCCHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHCCC
QINDTDAWDKASRNVVYAFQLHFRPQQPTGELDLETYAILKALNKKYAGRDDFY
EECCCCCCHHCCCCEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]