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The map label for this gene is rumA [H]

Identifier: 92113761

GI number: 92113761

Start: 1860973

End: 1862400

Strand: Direct

Name: rumA [H]

Synonym: Csal_1637

Alternate gene names: 92113761

Gene position: 1860973-1862400 (Clockwise)

Preceding gene: 92113760

Following gene: 92113762

Centisome position: 50.34

GC content: 70.31

Gene sequence:

>1428_bases
ATGGCGATGCTGGGCAAACGGCGTCCACCGCGCACCGCCAACGAACGCGTGCGTCGGGAGCGCGGGTCGGCAACACGGCG
CGACGACGCGACTGCCGACGGCCTGTCCATCGAGCGTCTGGCGCACGATGGACGCGGCGTGGCGCGCGATCCGCACGGCA
AGACGGTCTTCGTGGACCAGGCCCTGCCCGGCGAACGGGTACGGGTCGCCGTGCATCGTCAACGCAAGCGCTTCGACGAG
GCGCACGTCGTCGAGCGCCTGGCGACGTCCCCCGAGCGGGCGACACCGCCTTGTGCGTATTACGGGCGCTGCGGCGGCTG
CGATCTGCAGCATCTCGATCTCGAGGCGCAGCGCGAGCACAAGCGCCGTACGCTGGACGAGCTGTTCACCCGCCAGGGAC
TGACCCTGCCCGAGATAGAGGTGCTGGCCGGCGAAGGGCTGGCATACCGGCGCCGGGCGCGCCTGGGCGTCAAATGCGAT
GCCGAAGGTACGCCGCACCTGGGGTTTCGCGCCCGGGGCAGCGAGCACTTGATCGATATCGACAGCTGCGTGGTGCTCGA
GCCCGTGCTGTCACGGCTGATCGCGCCGCTGCGTGCATGCCTGGTGCAACTGGAAGCGCCCCGGCGCGTGGGTCACATCG
AGCTGATCGCCACCGCCGAGGGTGCTTGCGTGGTGGTGCGTCAGCTCCGCGAGGTGCCCGGCGACGAAGCCCGCTGGCGC
CGGTTCGCCGCCGAGCACGATGTCACCCTGGCCTGGCGGGTGGGACGCGAGGCGCCGACGCTACGCTGGCTGCACGCGCC
GCGCGGCGAGGCCCTGCATGTCACCCTGGTACTGGACGACACCACACTGTCGCTCGGCTGCGTGCCGGGCGATTTCCTGC
AGATCAACGCCGACGTCAATGCCCGCCTGGTGCGCACGGCCCTGGCCTGGCTCGCGCCACGCGGGGAAGAGCGCGTCCTC
GATCTGTTCGCCGGCGTGGGCAACTTCACGCTCGCGCTGGCGCCCCGCGTCGCGTCGATCACCGGCATCGAGGGCAGCCC
CGCGATGGTCGAGCGTCTCGCCGACAATGCACGTCGCGCCGGGCTGCACCAGGTCGGTGCACAACAATCCGATCTGGCCA
CCCAGGTACCGGAGGTCGCGGCGGTCGACTGGGTGATCATGGACCCGCCGCGCGGCGGGGCGGAGGCCCTGTGCCGCGCG
CTCGCCGACAGTCCGGTGACGACGCTGCTCTACGTCTCCTGCGATCCCGCGGCGCTGGCACGCGATGCGGCACGCCTTGT
GCAAGGCGGCTACCGTATCCAGCGCGCCGCGCTGGCCGATATGTTTCCACACACCGCGCATTTGGAATCGATGCTGCTAC
TGACCCGCGACACGCCGCGCGGTCGGTCCACATCCGTAGAAAGGGAAGATCATGGTCAAGGTCCGTGA

Upstream 100 bases:

>100_bases
CGGATCGCCGAGCAGGTGGAAAACGCGGTGATCGTGTTCATCGTCTGCGACCGTGGCGACCGTTACCTGTCCACCGGCCT
GTTCGCCCCGGAGCGCTGAG

Downstream 100 bases:

>100_bases
AGACCAGCCGTTGACGCAGGCCGGCGAGGTCGATATCGCATTGTGGCTCAAGCGCCTCCAGGACGATGTCGCGCTGCGCG
ATGTCGAGGAGTTGCGCGAG

Product: RNA methyltransferase

Products: NA

Alternate protein names: 23S rRNA(M-5-U1939)-methyltransferase [H]

Number of amino acids: Translated: 475; Mature: 474

Protein sequence:

>475_residues
MAMLGKRRPPRTANERVRRERGSATRRDDATADGLSIERLAHDGRGVARDPHGKTVFVDQALPGERVRVAVHRQRKRFDE
AHVVERLATSPERATPPCAYYGRCGGCDLQHLDLEAQREHKRRTLDELFTRQGLTLPEIEVLAGEGLAYRRRARLGVKCD
AEGTPHLGFRARGSEHLIDIDSCVVLEPVLSRLIAPLRACLVQLEAPRRVGHIELIATAEGACVVVRQLREVPGDEARWR
RFAAEHDVTLAWRVGREAPTLRWLHAPRGEALHVTLVLDDTTLSLGCVPGDFLQINADVNARLVRTALAWLAPRGEERVL
DLFAGVGNFTLALAPRVASITGIEGSPAMVERLADNARRAGLHQVGAQQSDLATQVPEVAAVDWVIMDPPRGGAEALCRA
LADSPVTTLLYVSCDPAALARDAARLVQGGYRIQRAALADMFPHTAHLESMLLLTRDTPRGRSTSVEREDHGQGP

Sequences:

>Translated_475_residues
MAMLGKRRPPRTANERVRRERGSATRRDDATADGLSIERLAHDGRGVARDPHGKTVFVDQALPGERVRVAVHRQRKRFDE
AHVVERLATSPERATPPCAYYGRCGGCDLQHLDLEAQREHKRRTLDELFTRQGLTLPEIEVLAGEGLAYRRRARLGVKCD
AEGTPHLGFRARGSEHLIDIDSCVVLEPVLSRLIAPLRACLVQLEAPRRVGHIELIATAEGACVVVRQLREVPGDEARWR
RFAAEHDVTLAWRVGREAPTLRWLHAPRGEALHVTLVLDDTTLSLGCVPGDFLQINADVNARLVRTALAWLAPRGEERVL
DLFAGVGNFTLALAPRVASITGIEGSPAMVERLADNARRAGLHQVGAQQSDLATQVPEVAAVDWVIMDPPRGGAEALCRA
LADSPVTTLLYVSCDPAALARDAARLVQGGYRIQRAALADMFPHTAHLESMLLLTRDTPRGRSTSVEREDHGQGP
>Mature_474_residues
AMLGKRRPPRTANERVRRERGSATRRDDATADGLSIERLAHDGRGVARDPHGKTVFVDQALPGERVRVAVHRQRKRFDEA
HVVERLATSPERATPPCAYYGRCGGCDLQHLDLEAQREHKRRTLDELFTRQGLTLPEIEVLAGEGLAYRRRARLGVKCDA
EGTPHLGFRARGSEHLIDIDSCVVLEPVLSRLIAPLRACLVQLEAPRRVGHIELIATAEGACVVVRQLREVPGDEARWRR
FAAEHDVTLAWRVGREAPTLRWLHAPRGEALHVTLVLDDTTLSLGCVPGDFLQINADVNARLVRTALAWLAPRGEERVLD
LFAGVGNFTLALAPRVASITGIEGSPAMVERLADNARRAGLHQVGAQQSDLATQVPEVAAVDWVIMDPPRGGAEALCRAL
ADSPVTTLLYVSCDPAALARDAARLVQGGYRIQRAALADMFPHTAHLESMLLLTRDTPRGRSTSVEREDHGQGP

Specific function: Catalyzes the formation of 5-methyl-uridine at position 1939 (M-5-U1939) in 23S rRNA [H]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI34222389, Length=195, Percent_Identity=31.2820512820513, Blast_Score=70, Evalue=4e-12,
Organism=Homo sapiens, GI51173878, Length=195, Percent_Identity=31.2820512820513, Blast_Score=70, Evalue=4e-12,
Organism=Escherichia coli, GI1789148, Length=428, Percent_Identity=39.018691588785, Blast_Score=280, Evalue=2e-76,
Organism=Escherichia coli, GI1787083, Length=402, Percent_Identity=28.1094527363184, Blast_Score=92, Evalue=6e-20,
Organism=Drosophila melanogaster, GI24666579, Length=203, Percent_Identity=27.5862068965517, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR002792
- InterPro:   IPR010280 [H]

Pfam domain/function: PF01938 TRAM; PF05958 tRNA_U5-meth_tr [H]

EC number: =2.1.1.190 [H]

Molecular weight: Translated: 52156; Mature: 52025

Theoretical pI: Translated: 8.14; Mature: 8.14

Prosite motif: PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAMLGKRRPPRTANERVRRERGSATRRDDATADGLSIERLAHDGRGVARDPHGKTVFVDQ
CCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCEEEEEC
ALPGERVRVAVHRQRKRFDEAHVVERLATSPERATPPCAYYGRCGGCDLQHLDLEAQREH
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCCCCCCCCCCCHHHHHH
KRRTLDELFTRQGLTLPEIEVLAGEGLAYRRRARLGVKCDAEGTPHLGFRARGSEHLIDI
HHHHHHHHHHHCCCCCCCCCEEECCCHHHHHHHCCCEEECCCCCCCCCEECCCCCCEEEC
DSCVVLEPVLSRLIAPLRACLVQLEAPRRVGHIELIATAEGACVVVRQLREVPGDEARWR
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHCCCCHHHHH
RFAAEHDVTLAWRVGREAPTLRWLHAPRGEALHVTLVLDDTTLSLGCVPGDFLQINADVN
HHHHCCCCEEEEEECCCCCCEEEEECCCCCEEEEEEEEECCEEEEECCCCCEEEEECCCC
ARLVRTALAWLAPRGEERVLDLFAGVGNFTLALAPRVASITGIEGSPAMVERLADNARRA
HHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEEECCCHHEEECCCCCHHHHHHHHHHHHHC
GLHQVGAQQSDLATQVPEVAAVDWVIMDPPRGGAEALCRALADSPVTTLLYVSCDPAALA
CHHHHCCCHHHHHHHCCCHHEEEEEEECCCCCCHHHHHHHHCCCCCEEEEEEECCHHHHH
RDAARLVQGGYRIQRAALADMFPHTAHLESMLLLTRDTPRGRSTSVEREDHGQGP
HHHHHHHHCCHHHHHHHHHHHCCCHHHHHHEEEEECCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AMLGKRRPPRTANERVRRERGSATRRDDATADGLSIERLAHDGRGVARDPHGKTVFVDQ
CCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCEEEEEC
ALPGERVRVAVHRQRKRFDEAHVVERLATSPERATPPCAYYGRCGGCDLQHLDLEAQREH
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCCCCCCCCCCCHHHHHH
KRRTLDELFTRQGLTLPEIEVLAGEGLAYRRRARLGVKCDAEGTPHLGFRARGSEHLIDI
HHHHHHHHHHHCCCCCCCCCEEECCCHHHHHHHCCCEEECCCCCCCCCEECCCCCCEEEC
DSCVVLEPVLSRLIAPLRACLVQLEAPRRVGHIELIATAEGACVVVRQLREVPGDEARWR
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHCCCCHHHHH
RFAAEHDVTLAWRVGREAPTLRWLHAPRGEALHVTLVLDDTTLSLGCVPGDFLQINADVN
HHHHCCCCEEEEEECCCCCCEEEEECCCCCEEEEEEEEECCEEEEECCCCCEEEEECCCC
ARLVRTALAWLAPRGEERVLDLFAGVGNFTLALAPRVASITGIEGSPAMVERLADNARRA
HHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEEECCCHHEEECCCCCHHHHHHHHHHHHHC
GLHQVGAQQSDLATQVPEVAAVDWVIMDPPRGGAEALCRALADSPVTTLLYVSCDPAALA
CHHHHCCCHHHHHHHCCCHHEEEEEEECCCCCCHHHHHHHHCCCCCEEEEEEECCHHHHH
RDAARLVQGGYRIQRAALADMFPHTAHLESMLLLTRDTPRGRSTSVEREDHGQGP
HHHHHHHHCCHHHHHHHHHHHCCCHHHHHHEEEEECCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA