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The map label for this gene is dmlR [H]

Identifier: 91790325

GI number: 91790325

Start: 4747685

End: 4748608

Strand: Reverse

Name: dmlR [H]

Synonym: Bpro_4493

Alternate gene names: 91790325

Gene position: 4748608-4747685 (Counterclockwise)

Preceding gene: 91790327

Following gene: 91790323

Centisome position: 91.31

GC content: 68.29

Gene sequence:

>924_bases
ATGGACAAACTCAAGCAACTGGAATCATTTGTGGCGGTGGTCGCGCGCGGCAGTCTGACGGCGGCGGCCAAGGCGGAGGG
GGTGGCCCCGGCCATCATGGGCCGGCGGCTGGATGCGCTGGAGGAGCGGCTGGGCGTGAAGCTGCTGGTGCGCACCACGC
GGCGCATCACGCTCACCCACGAAGGCAGCGCTTTCCTCGAGGATTGCCAGCGCCTGCTGGCCGACCTGGCCAACGCCGAG
GCCAGCGTGTCGGCGGGTGGGGTGAAAGCCAGTGGCCATTTGCGCATCACCGCGCCTGCCGGTTTTGGCCGGCGCCATGT
GGCGCCGCTGGTGACGAAGTTTCGCGAGCAGCATCTGGACGTCACGATTTCGCTGAACCTGAGCGACCGCGTGGTGGACC
TGGCGGGCGAGGGTTTTGACTGCGCGGTGCGCGTGGGCGACCTGCCCGATTCGTCGCTGGTCAGCGTGCGCATGGCCGAC
AACCGGCGCCTGTGTGTCGCGACGCCGCGCTACCTCCAGCGCCACGGCACGCCGCAGCATCCCGCCGAGCTGGCCCGGTT
TGACTGCCTCACCCTGTCCAGCGACGCGTCCCAAACCCGTGGCTGGGCCTTCCAGGTCAACGGCGAGCTGATACACCTGA
AACCCGGCGGGCCGCTGGACTGCTCGGACGGTCAGGTACTGCACGACTGGTGCCTGGCCGACTACGGCATTGCCTGGCGC
AGCACCTGGGAGGTCGAATCGGAAATCGCCAGCGGCCACCTGGTGGCCGTGCTGGAAGCGTTTGCGGCGCCGCCCAACGG
CATCTACGCCGTCTTTCCGCAGCGCAAGCATTTGCCGCTGCGCGTGCGCCTCTGGATAGACTTCCTGAAGCACCATTACA
GCCAGCCGGATTTCTGGCGCTCGGCGGCGGCCTCGCCCGCGTAG

Upstream 100 bases:

>100_bases
CGCAAATGTCCCGTACCCGTCATGTAACTTGCCGCTCAAAAAACGCTTTAATGAGCTTCAAAAAATGTCCTATTCAGAAC
ATTTATATACATAATATGGG

Downstream 100 bases:

>100_bases
TCCGCTTGGGCGCTTTGTGTTCGCCAGCTTAAAGCAGGCCGATCAGCAGCCAGCCGGCCGTGCCCCACATCATCAGCGCC
ACCAGGCCATCGAGCGCGCG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: D-malate degradation protein R [H]

Number of amino acids: Translated: 307; Mature: 307

Protein sequence:

>307_residues
MDKLKQLESFVAVVARGSLTAAAKAEGVAPAIMGRRLDALEERLGVKLLVRTTRRITLTHEGSAFLEDCQRLLADLANAE
ASVSAGGVKASGHLRITAPAGFGRRHVAPLVTKFREQHLDVTISLNLSDRVVDLAGEGFDCAVRVGDLPDSSLVSVRMAD
NRRLCVATPRYLQRHGTPQHPAELARFDCLTLSSDASQTRGWAFQVNGELIHLKPGGPLDCSDGQVLHDWCLADYGIAWR
STWEVESEIASGHLVAVLEAFAAPPNGIYAVFPQRKHLPLRVRLWIDFLKHHYSQPDFWRSAAASPA

Sequences:

>Translated_307_residues
MDKLKQLESFVAVVARGSLTAAAKAEGVAPAIMGRRLDALEERLGVKLLVRTTRRITLTHEGSAFLEDCQRLLADLANAE
ASVSAGGVKASGHLRITAPAGFGRRHVAPLVTKFREQHLDVTISLNLSDRVVDLAGEGFDCAVRVGDLPDSSLVSVRMAD
NRRLCVATPRYLQRHGTPQHPAELARFDCLTLSSDASQTRGWAFQVNGELIHLKPGGPLDCSDGQVLHDWCLADYGIAWR
STWEVESEIASGHLVAVLEAFAAPPNGIYAVFPQRKHLPLRVRLWIDFLKHHYSQPDFWRSAAASPA
>Mature_307_residues
MDKLKQLESFVAVVARGSLTAAAKAEGVAPAIMGRRLDALEERLGVKLLVRTTRRITLTHEGSAFLEDCQRLLADLANAE
ASVSAGGVKASGHLRITAPAGFGRRHVAPLVTKFREQHLDVTISLNLSDRVVDLAGEGFDCAVRVGDLPDSSLVSVRMAD
NRRLCVATPRYLQRHGTPQHPAELARFDCLTLSSDASQTRGWAFQVNGELIHLKPGGPLDCSDGQVLHDWCLADYGIAWR
STWEVESEIASGHLVAVLEAFAAPPNGIYAVFPQRKHLPLRVRLWIDFLKHHYSQPDFWRSAAASPA

Specific function: Transcriptional regulator required for the aerobic growth on D-malate as the sole carbon source. Induces the expression of dmlA in response to D-malate or L- or meso-tartrate. Negatively regulates its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081978, Length=298, Percent_Identity=36.241610738255, Blast_Score=189, Evalue=2e-49,
Organism=Escherichia coli, GI1786401, Length=289, Percent_Identity=33.9100346020761, Blast_Score=158, Evalue=4e-40,
Organism=Escherichia coli, GI1789440, Length=287, Percent_Identity=32.0557491289199, Blast_Score=155, Evalue=2e-39,
Organism=Escherichia coli, GI145693193, Length=292, Percent_Identity=30.1369863013699, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI1789639, Length=298, Percent_Identity=29.8657718120805, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI1787128, Length=289, Percent_Identity=24.9134948096886, Blast_Score=121, Evalue=5e-29,
Organism=Escherichia coli, GI1787589, Length=292, Percent_Identity=27.7397260273973, Blast_Score=115, Evalue=5e-27,
Organism=Escherichia coli, GI1790011, Length=297, Percent_Identity=25.5892255892256, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1786448, Length=277, Percent_Identity=27.0758122743682, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1788706, Length=268, Percent_Identity=25.3731343283582, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1789173, Length=269, Percent_Identity=25.2788104089219, Blast_Score=69, Evalue=5e-13,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33566; Mature: 33566

Theoretical pI: Translated: 7.92; Mature: 7.92

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKLKQLESFVAVVARGSLTAAAKAEGVAPAIMGRRLDALEERLGVKLLVRTTRRITLTH
CCHHHHHHHHHHHHHCCCCCCHHHCCCCCHHHHHHHHHHHHHHHCEEEEEECCEEEEEEE
EGSAFLEDCQRLLADLANAEASVSAGGVKASGHLRITAPAGFGRRHVAPLVTKFREQHLD
CCCHHHHHHHHHHHHHHCCCCCCCCCCEEECCEEEEEECCCCCCHHHHHHHHHHHHHHCC
VTISLNLSDRVVDLAGEGFDCAVRVGDLPDSSLVSVRMADNRRLCVATPRYLQRHGTPQH
EEEEECCCCEEEEECCCCCEEEEEECCCCCCCEEEEEECCCCEEEEECHHHHHHCCCCCC
PAELARFDCLTLSSDASQTRGWAFQVNGELIHLKPGGPLDCSDGQVLHDWCLADYGIAWR
HHHHHHHHEEEECCCCCCCCCEEEEECCEEEEECCCCCCCCCCCCHHHHHHHHHCCCCCC
STWEVESEIASGHLVAVLEAFAAPPNGIYAVFPQRKHLPLRVRLWIDFLKHHYSQPDFWR
CCCHHHHHHHCCHHHHHHHHHCCCCCCEEEECCCCCCCCEEHHHHHHHHHHHCCCCCHHH
SAAASPA
HCCCCCC
>Mature Secondary Structure
MDKLKQLESFVAVVARGSLTAAAKAEGVAPAIMGRRLDALEERLGVKLLVRTTRRITLTH
CCHHHHHHHHHHHHHCCCCCCHHHCCCCCHHHHHHHHHHHHHHHCEEEEEECCEEEEEEE
EGSAFLEDCQRLLADLANAEASVSAGGVKASGHLRITAPAGFGRRHVAPLVTKFREQHLD
CCCHHHHHHHHHHHHHHCCCCCCCCCCEEECCEEEEEECCCCCCHHHHHHHHHHHHHHCC
VTISLNLSDRVVDLAGEGFDCAVRVGDLPDSSLVSVRMADNRRLCVATPRYLQRHGTPQH
EEEEECCCCEEEEECCCCCEEEEEECCCCCCCEEEEEECCCCEEEEECHHHHHHCCCCCC
PAELARFDCLTLSSDASQTRGWAFQVNGELIHLKPGGPLDCSDGQVLHDWCLADYGIAWR
HHHHHHHHEEEECCCCCCCCCEEEEECCEEEEECCCCCCCCCCCCHHHHHHHHHCCCCCC
STWEVESEIASGHLVAVLEAFAAPPNGIYAVFPQRKHLPLRVRLWIDFLKHHYSQPDFWR
CCCHHHHHHHCCHHHHHHHHHCCCCCCEEEECCCCCCCCEEHHHHHHHHHHHCCCCCHHH
SAAASPA
HCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]