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The map label for this gene is emrA [H]

Identifier: 91789095

GI number: 91789095

Start: 3423441

End: 3424838

Strand: Reverse

Name: emrA [H]

Synonym: Bpro_3235

Alternate gene names: 91789095

Gene position: 3424838-3423441 (Counterclockwise)

Preceding gene: 91789096

Following gene: 91789094

Centisome position: 65.86

GC content: 67.74

Gene sequence:

>1398_bases
ATGACAACTCCAGAACCCTCGCTATCCGCAGCTTCTTCTGACGCACCCTTGCAACCGGCCGCCGGCAACCCCCGGCGCAA
GAAAGCGCTGACCGCGCTGGCCAGCGTGGTGGTGGTCGCCGGCCTGGGCTGGGCCGCCTACGAGTACCTCGTGGCCAGCC
ATTACGAGTCGACCGACAACGCTTACGTGCAAGGCAACGTGATCCAGATCACGCCGCAGATCGGCGGCACCGTCATGGCC
ATCATGGCCGACGACACCGACTTCGTGAAAGCCGGTCAGACGCTGGTGCAACTGGACCCGGCAGACGCCAGAATCGCGCT
GGAGCAGGCCGAAGCCGCGCTGGCCCAGGCGGTGCGCCAGGTGCGCACGCTCTACGCCAACAACGGCACGCTGGCCGCCC
AGATCGCGCTGCGTGATGCCGACGTGGCCAGGGCCCGCAGCGATATTGCCCGCGCCACCGATGACCTCAACCGCCGCCAG
TCGCTCACCGGCAACGGGGCGGTGTCGAAAGAAGAACTCAACCATGCGCAGACGCAGCTGGCCAACGCCAAAAGCGCGCT
GGCCGCAGCGCAGGCCGGCGTGGTGGCCGCGCGCGAGCAGCTCACCAGCAACCAGGCCTTGACCGAGGGGACCAGCATCG
AGCAGCACCCCAGCGTGCAGGTGGCGGCCGCCAAGGTGCGCGAGGCCTACCTCGCCACACAGCGCGCCGGTCTGCAGGCT
CCGGTGGATGGCTACGTGGCCAAGCGCACGGTGCAGCTGGGCCAGCGCGTGGCGGCCGGCACGCCCATGATGTCGATCAT
TCCGCTCAACCAGGTCTGGGTTGATGCCAACTTCAAGGAAGTGCAACTGCGCAATATCCGCATCGGCCAGCCGGTGACGC
TGGTGGCCGACCTGTATGGCAAAAAAATCCAATACAAAGGCACGGTAGCGGGGCTGGGTGTCGGCACGGGCGCGGCCTTT
TCGCTGCTGCCGGCGCAAAACGCCACCGGCAACTGGATCAAGGTGGTGCAGCGCGTGCCGGTGCGCATTGCGCTCGACGC
CAGGCAACTGGCCGACAACCCGCTGCGGGTGGGCTTGTCGATGGATGCGGAAGTCGACGTCAGCCACAAGGACGGCAAAA
CGCTGGCCGATGCGCCGCGCGACGCGGCCCAGGCGCACACGCAGACTTATGGCAAGCAGGACGATGCCGCCAACGTGTAT
GTGCGGCGCGTGATTGCCGCCAACAGCGGCAAGGCACTCAAACCGGCCAAAGCCGCCGCCATCGGCTCGCCGGCCCAACC
GCCGGGCGGCCCCACCACAGCGGATCAGACCGGCAGCGAAGGGCAGGGCCTTGCGATGGCGCAGACGCAGACCCTGTCGA
TTGCCGGCAGCCGCGCGGCACCACAGCAGGCGCACTAA

Upstream 100 bases:

>100_bases
CAAACGCTATAAAAACCCGTAGCTTCTTTCGCCCGTGTTTGTTGGGCCAGAGGCCTGGTAGACCTAATTGACCTAATTGA
CTTAATTGATCAGGAACATC

Downstream 100 bases:

>100_bases
GCCCCGTTCGCCGGTCATTGCATTACCCGCAGCTTCGCACGGCGCGCACCTCCACGGCCTTGTCCTGCTGCTTACTCATT
AGGTTTTCATGGCTTCAGCA

Product: secretion protein HlyD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 465; Mature: 464

Protein sequence:

>465_residues
MTTPEPSLSAASSDAPLQPAAGNPRRKKALTALASVVVVAGLGWAAYEYLVASHYESTDNAYVQGNVIQITPQIGGTVMA
IMADDTDFVKAGQTLVQLDPADARIALEQAEAALAQAVRQVRTLYANNGTLAAQIALRDADVARARSDIARATDDLNRRQ
SLTGNGAVSKEELNHAQTQLANAKSALAAAQAGVVAAREQLTSNQALTEGTSIEQHPSVQVAAAKVREAYLATQRAGLQA
PVDGYVAKRTVQLGQRVAAGTPMMSIIPLNQVWVDANFKEVQLRNIRIGQPVTLVADLYGKKIQYKGTVAGLGVGTGAAF
SLLPAQNATGNWIKVVQRVPVRIALDARQLADNPLRVGLSMDAEVDVSHKDGKTLADAPRDAAQAHTQTYGKQDDAANVY
VRRVIAANSGKALKPAKAAAIGSPAQPPGGPTTADQTGSEGQGLAMAQTQTLSIAGSRAAPQQAH

Sequences:

>Translated_465_residues
MTTPEPSLSAASSDAPLQPAAGNPRRKKALTALASVVVVAGLGWAAYEYLVASHYESTDNAYVQGNVIQITPQIGGTVMA
IMADDTDFVKAGQTLVQLDPADARIALEQAEAALAQAVRQVRTLYANNGTLAAQIALRDADVARARSDIARATDDLNRRQ
SLTGNGAVSKEELNHAQTQLANAKSALAAAQAGVVAAREQLTSNQALTEGTSIEQHPSVQVAAAKVREAYLATQRAGLQA
PVDGYVAKRTVQLGQRVAAGTPMMSIIPLNQVWVDANFKEVQLRNIRIGQPVTLVADLYGKKIQYKGTVAGLGVGTGAAF
SLLPAQNATGNWIKVVQRVPVRIALDARQLADNPLRVGLSMDAEVDVSHKDGKTLADAPRDAAQAHTQTYGKQDDAANVY
VRRVIAANSGKALKPAKAAAIGSPAQPPGGPTTADQTGSEGQGLAMAQTQTLSIAGSRAAPQQAH
>Mature_464_residues
TTPEPSLSAASSDAPLQPAAGNPRRKKALTALASVVVVAGLGWAAYEYLVASHYESTDNAYVQGNVIQITPQIGGTVMAI
MADDTDFVKAGQTLVQLDPADARIALEQAEAALAQAVRQVRTLYANNGTLAAQIALRDADVARARSDIARATDDLNRRQS
LTGNGAVSKEELNHAQTQLANAKSALAAAQAGVVAAREQLTSNQALTEGTSIEQHPSVQVAAAKVREAYLATQRAGLQAP
VDGYVAKRTVQLGQRVAAGTPMMSIIPLNQVWVDANFKEVQLRNIRIGQPVTLVADLYGKKIQYKGTVAGLGVGTGAAFS
LLPAQNATGNWIKVVQRVPVRIALDARQLADNPLRVGLSMDAEVDVSHKDGKTLADAPRDAAQAHTQTYGKQDDAANVYV
RRVIAANSGKALKPAKAAAIGSPAQPPGGPTTADQTGSEGQGLAMAQTQTLSIAGSRAAPQQAH

Specific function: The emr locus confers resistance to substances of high hydrophobicity. EmrA probably participate in a transport system to extrude toxins and drugs from the cell [H]

COG id: COG1566

COG function: function code V; Multidrug resistance efflux pump

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789041, Length=400, Percent_Identity=44.25, Blast_Score=337, Evalue=1e-93,
Organism=Escherichia coli, GI1788711, Length=358, Percent_Identity=43.0167597765363, Blast_Score=292, Evalue=3e-80,
Organism=Escherichia coli, GI1790519, Length=301, Percent_Identity=31.2292358803987, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI87081951, Length=324, Percent_Identity=26.5432098765432, Blast_Score=103, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005694
- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 48486; Mature: 48355

Theoretical pI: Translated: 9.65; Mature: 9.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTPEPSLSAASSDAPLQPAAGNPRRKKALTALASVVVVAGLGWAAYEYLVASHYESTDN
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCC
AYVQGNVIQITPQIGGTVMAIMADDTDFVKAGQTLVQLDPADARIALEQAEAALAQAVRQ
EEEECCEEEEECCCCCEEEEEEECCCHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHH
VRTLYANNGTLAAQIALRDADVARARSDIARATDDLNRRQSLTGNGAVSKEELNHAQTQL
HHHHHCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
ANAKSALAAAQAGVVAAREQLTSNQALTEGTSIEQHPSVQVAAAKVREAYLATQRAGLQA
HHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCC
PVDGYVAKRTVQLGQRVAAGTPMMSIIPLNQVWVDANFKEVQLRNIRIGQPVTLVADLYG
CCHHHHHHHHHHHHHHHHCCCCCEEEEECCEEEECCCCCEEEEEEEECCCCHHHHHHHHC
KKIQYKGTVAGLGVGTGAAFSLLPAQNATGNWIKVVQRVPVRIALDARQLADNPLRVGLS
CEEEECCCEEECCCCCCCEEEEECCCCCCCHHHHHHHHCCEEEEECHHHHCCCCEEEEEE
MDAEVDVSHKDGKTLADAPRDAAQAHTQTYGKQDDAANVYVRRVIAANSGKALKPAKAAA
CCCEEEECCCCCCCHHCCCHHHHHHHHHHCCCCCCHHHHHHHEEEECCCCCCCCCHHHHH
IGSPAQPPGGPTTADQTGSEGQGLAMAQTQTLSIAGSRAAPQQAH
CCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEEEECCCCCCCCCC
>Mature Secondary Structure 
TTPEPSLSAASSDAPLQPAAGNPRRKKALTALASVVVVAGLGWAAYEYLVASHYESTDN
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCC
AYVQGNVIQITPQIGGTVMAIMADDTDFVKAGQTLVQLDPADARIALEQAEAALAQAVRQ
EEEECCEEEEECCCCCEEEEEEECCCHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHH
VRTLYANNGTLAAQIALRDADVARARSDIARATDDLNRRQSLTGNGAVSKEELNHAQTQL
HHHHHCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
ANAKSALAAAQAGVVAAREQLTSNQALTEGTSIEQHPSVQVAAAKVREAYLATQRAGLQA
HHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCC
PVDGYVAKRTVQLGQRVAAGTPMMSIIPLNQVWVDANFKEVQLRNIRIGQPVTLVADLYG
CCHHHHHHHHHHHHHHHHCCCCCEEEEECCEEEECCCCCEEEEEEEECCCCHHHHHHHHC
KKIQYKGTVAGLGVGTGAAFSLLPAQNATGNWIKVVQRVPVRIALDARQLADNPLRVGLS
CEEEECCCEEECCCCCCCEEEEECCCCCCCHHHHHHHHCCEEEEECHHHHCCCCEEEEEE
MDAEVDVSHKDGKTLADAPRDAAQAHTQTYGKQDDAANVYVRRVIAANSGKALKPAKAAA
CCCEEEECCCCCCCHHCCCHHHHHHHHHHCCCCCCHHHHHHHEEEECCCCCCCCCHHHHH
IGSPAQPPGGPTTADQTGSEGQGLAMAQTQTLSIAGSRAAPQQAH
CCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1409590; 9205837; 9278503; 8203018 [H]