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The map label for this gene is emrB [H]

Identifier: 91789094

GI number: 91789094

Start: 3421784

End: 3423352

Strand: Reverse

Name: emrB [H]

Synonym: Bpro_3234

Alternate gene names: 91789094

Gene position: 3423352-3421784 (Counterclockwise)

Preceding gene: 91789095

Following gene: 91789091

Centisome position: 65.83

GC content: 63.29

Gene sequence:

>1569_bases
ATGGCTTCAGCACCTGCCCCTGCCTACATCCAGCCGCCGCCGCTGACGGGCTCTGCCCGCCTGTGGGGCACGCTGGCGCT
GTCGGCCGCCACCTTCATGAACGTGCTCGACACGTCGATTGCCAACGTCTCCCTGCCGGCCATTGCCGGCGACCTGGGCG
TGAGCCCCAACCAGGGCACCTGGGTCATCACCAGCTTTGGCGTGGCCAACGCGATCGCCGTGCCGCTCACCGGCTGGCTG
TCGCAGCGTTTCGGCCAGGTTCGCCTGTTCACGGCCAGCGTCATGCTGTTTGTGCTCACATCATGGCTGTGCGGCCTGGC
CCCCAACATGACCACGCTGATTTTTTTTCGTGTCCTGCAGGGGCTGGTGGCGGGCCCGATGATTCCGTTGTCGCAGTCAC
TGCTGCTGTCAAGCTACCCCAAGGCGCTGGCGGGACTGGCCATGGCGCTCTGGTCCATGACCACGCTGGTCGCGCCTGTC
ATGGGGCCGCTGCTGGGCGGCTGGATCACCGACAACATTTCCTGGCCCTGGATTTTTTACATCAACATTCCCGTGGGCTT
TGGCGCCGCCTTCATCACCTGGAGCATCTTCAGGAAGCGTGAAAGCGTGACGCGCAAGCTGCCGATCGACTCGGTCGGCC
TGGCGCTGCTGGTGATCTGGGTGGGTGCGCTGCAGATCATGCTCGACAAGGGCAAGGAGCTCGACTGGTTTCACTCCGGT
GAGGTGGTGGCCCTGGGCATTGTTTCGCTGGTGGGCCTGATTGCCTTCGTCATCTGGGAGCTCACCGAAGAGCATCCGGT
GGTTGACCTGACGCTGTTCAAGCGGCGCAACTTCTGGGCGGGTGCGGTGGCGCTGTCGGTGGGGTACGGCCTCTTTTTTG
GCAACGTGGTGCTGCTGCCGCTGTGGCTGCAGCAGTACATGGGCTACACCGCCACACAGGCCGGCATGGTGATGGCGCCG
GTGGGGGCCATGGCTCTGCTGCTGTCACCGCTGGTCGGCAAAAACATCGCCAAGGTCGACCCGCGCCGCTTCGCCGTGTT
TGCCTTTCTGGTGTTTGCGCTGGTGCTGTGGATGCGTTCCAACTTCAACACCCAGGCTGATTTCAACACCATCCTGATAC
CCACCATTATCCAGGGTGTGGCCATGGCTTTCTTCTTCATCCCGTTGGTCACCATCACGCTGTCGGGCATCTCGCCCGAG
AAGATTCCGGCGGCTTCGGGCCTGTCGAACTTCGTGCGCATCACGGCGGGCTCCTTCGGCACGTCAATCGCCACCACCAT
CTGGCAAGACCGCGCCGCCATGCACCATGCCCAAGTGAGCGAATACGTCAACCAGGGAAGCACCGCCACCAGCAGCGCAC
TGACGGGCCTGGCCGGGGCCGGGCTTACACCCGACCAGGCACTGGGCACCATCAATCGCCTGGTGGATCAGCAGGCCTAC
ATGCTGGCGGCGAACGATGTGTTCTATGTGTCCGCGCTGATTTTCCTGTTGCTGATTCCGCTGGTGTATTTGACGCGGCA
CACCAGGGTCGGGGCAGGGAGTTCGGATGCTGCGGCTGGGGCGCATTGA

Upstream 100 bases:

>100_bases
CAGGCGCACTAAGCCCCGTTCGCCGGTCATTGCATTACCCGCAGCTTCGCACGGCGCGCACCTCCACGGCCTTGTCCTGC
TGCTTACTCATTAGGTTTTC

Downstream 100 bases:

>100_bases
GGAACGACATTGGCAAACGGTTACTTTTTTTTCAGGCTTTCCCGACATTTCCCGTTTTTGAAGGCCGGGCCGCCTGCTGG
TTGCCCACGGCGCCTTTGGC

Product: EmrB/QacA family drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 522; Mature: 521

Protein sequence:

>522_residues
MASAPAPAYIQPPPLTGSARLWGTLALSAATFMNVLDTSIANVSLPAIAGDLGVSPNQGTWVITSFGVANAIAVPLTGWL
SQRFGQVRLFTASVMLFVLTSWLCGLAPNMTTLIFFRVLQGLVAGPMIPLSQSLLLSSYPKALAGLAMALWSMTTLVAPV
MGPLLGGWITDNISWPWIFYINIPVGFGAAFITWSIFRKRESVTRKLPIDSVGLALLVIWVGALQIMLDKGKELDWFHSG
EVVALGIVSLVGLIAFVIWELTEEHPVVDLTLFKRRNFWAGAVALSVGYGLFFGNVVLLPLWLQQYMGYTATQAGMVMAP
VGAMALLLSPLVGKNIAKVDPRRFAVFAFLVFALVLWMRSNFNTQADFNTILIPTIIQGVAMAFFFIPLVTITLSGISPE
KIPAASGLSNFVRITAGSFGTSIATTIWQDRAAMHHAQVSEYVNQGSTATSSALTGLAGAGLTPDQALGTINRLVDQQAY
MLAANDVFYVSALIFLLLIPLVYLTRHTRVGAGSSDAAAGAH

Sequences:

>Translated_522_residues
MASAPAPAYIQPPPLTGSARLWGTLALSAATFMNVLDTSIANVSLPAIAGDLGVSPNQGTWVITSFGVANAIAVPLTGWL
SQRFGQVRLFTASVMLFVLTSWLCGLAPNMTTLIFFRVLQGLVAGPMIPLSQSLLLSSYPKALAGLAMALWSMTTLVAPV
MGPLLGGWITDNISWPWIFYINIPVGFGAAFITWSIFRKRESVTRKLPIDSVGLALLVIWVGALQIMLDKGKELDWFHSG
EVVALGIVSLVGLIAFVIWELTEEHPVVDLTLFKRRNFWAGAVALSVGYGLFFGNVVLLPLWLQQYMGYTATQAGMVMAP
VGAMALLLSPLVGKNIAKVDPRRFAVFAFLVFALVLWMRSNFNTQADFNTILIPTIIQGVAMAFFFIPLVTITLSGISPE
KIPAASGLSNFVRITAGSFGTSIATTIWQDRAAMHHAQVSEYVNQGSTATSSALTGLAGAGLTPDQALGTINRLVDQQAY
MLAANDVFYVSALIFLLLIPLVYLTRHTRVGAGSSDAAAGAH
>Mature_521_residues
ASAPAPAYIQPPPLTGSARLWGTLALSAATFMNVLDTSIANVSLPAIAGDLGVSPNQGTWVITSFGVANAIAVPLTGWLS
QRFGQVRLFTASVMLFVLTSWLCGLAPNMTTLIFFRVLQGLVAGPMIPLSQSLLLSSYPKALAGLAMALWSMTTLVAPVM
GPLLGGWITDNISWPWIFYINIPVGFGAAFITWSIFRKRESVTRKLPIDSVGLALLVIWVGALQIMLDKGKELDWFHSGE
VVALGIVSLVGLIAFVIWELTEEHPVVDLTLFKRRNFWAGAVALSVGYGLFFGNVVLLPLWLQQYMGYTATQAGMVMAPV
GAMALLLSPLVGKNIAKVDPRRFAVFAFLVFALVLWMRSNFNTQADFNTILIPTIIQGVAMAFFFIPLVTITLSGISPEK
IPAASGLSNFVRITAGSFGTSIATTIWQDRAAMHHAQVSEYVNQGSTATSSALTGLAGAGLTPDQALGTINRLVDQQAYM
LAANDVFYVSALIFLLLIPLVYLTRHTRVGAGSSDAAAGAH

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=497, Percent_Identity=52.112676056338, Blast_Score=513, Evalue=1e-146,
Organism=Escherichia coli, GI1788710, Length=496, Percent_Identity=47.1774193548387, Blast_Score=451, Evalue=1e-128,
Organism=Escherichia coli, GI1790195, Length=466, Percent_Identity=28.5407725321888, Blast_Score=157, Evalue=2e-39,
Organism=Escherichia coli, GI87081983, Length=399, Percent_Identity=25.8145363408521, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1788392, Length=427, Percent_Identity=28.3372365339578, Blast_Score=113, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6322958, Length=177, Percent_Identity=27.683615819209, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6325455, Length=126, Percent_Identity=29.3650793650794, Blast_Score=67, Evalue=9e-12,
Organism=Saccharomyces cerevisiae, GI6321663, Length=150, Percent_Identity=27.3333333333333, Blast_Score=64, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 55869; Mature: 55738

Theoretical pI: Translated: 9.45; Mature: 9.45

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASAPAPAYIQPPPLTGSARLWGTLALSAATFMNVLDTSIANVSLPAIAGDLGVSPNQGT
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCCCCE
WVITSFGVANAIAVPLTGWLSQRFGQVRLFTASVMLFVLTSWLCGLAPNMTTLIFFRVLQ
EEEEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
GLVAGPMIPLSQSLLLSSYPKALAGLAMALWSMTTLVAPVMGPLLGGWITDNISWPWIFY
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCEEEE
INIPVGFGAAFITWSIFRKRESVTRKLPIDSVGLALLVIWVGALQIMLDKGKELDWFHSG
EECCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
EVVALGIVSLVGLIAFVIWELTEEHPVVDLTLFKRRNFWAGAVALSVGYGLFFGNVVLLP
CHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
LWLQQYMGYTATQAGMVMAPVGAMALLLSPLVGKNIAKVDPRRFAVFAFLVFALVLWMRS
HHHHHHCCCCHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHC
NFNTQADFNTILIPTIIQGVAMAFFFIPLVTITLSGISPEKIPAASGLSNFVRITAGSFG
CCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHEEECCCCC
TSIATTIWQDRAAMHHAQVSEYVNQGSTATSSALTGLAGAGLTPDQALGTINRLVDQQAY
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
MLAANDVFYVSALIFLLLIPLVYLTRHTRVGAGSSDAAAGAH
HEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
ASAPAPAYIQPPPLTGSARLWGTLALSAATFMNVLDTSIANVSLPAIAGDLGVSPNQGT
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCCCCE
WVITSFGVANAIAVPLTGWLSQRFGQVRLFTASVMLFVLTSWLCGLAPNMTTLIFFRVLQ
EEEEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
GLVAGPMIPLSQSLLLSSYPKALAGLAMALWSMTTLVAPVMGPLLGGWITDNISWPWIFY
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCEEEE
INIPVGFGAAFITWSIFRKRESVTRKLPIDSVGLALLVIWVGALQIMLDKGKELDWFHSG
EECCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
EVVALGIVSLVGLIAFVIWELTEEHPVVDLTLFKRRNFWAGAVALSVGYGLFFGNVVLLP
CHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
LWLQQYMGYTATQAGMVMAPVGAMALLLSPLVGKNIAKVDPRRFAVFAFLVFALVLWMRS
HHHHHHCCCCHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHC
NFNTQADFNTILIPTIIQGVAMAFFFIPLVTITLSGISPEKIPAASGLSNFVRITAGSFG
CCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHEEECCCCC
TSIATTIWQDRAAMHHAQVSEYVNQGSTATSSALTGLAGAGLTPDQALGTINRLVDQQAY
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
MLAANDVFYVSALIFLLLIPLVYLTRHTRVGAGSSDAAAGAH
HEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]