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The map label for this gene is yogA [H]

Identifier: 91788882

GI number: 91788882

Start: 3188493

End: 3189479

Strand: Reverse

Name: yogA [H]

Synonym: Bpro_3022

Alternate gene names: 91788882

Gene position: 3189479-3188493 (Counterclockwise)

Preceding gene: 91788883

Following gene: 91788881

Centisome position: 61.33

GC content: 66.36

Gene sequence:

>987_bases
ATGCAGTCTTACTGGATGCAGATGAACGCGGATCACGCCACGCTGGAACTGCGTGACGTGCCCCAGCCCGAGCCGGGGGC
CGGCCAGGTGCTGGTGCGGCTGCACGCCGCCAGCCTGAACCGCGGCGAGTTCATCGCCGGCCATGGCCTGCATGGCAAGC
CCGGCAGCGCCAAGGCTGTCGGCATGGAGGGGGCGGGCGAGGTGGTCGGGCTGGGTGCGGGCGTGCGCGGCCTGAAAGCA
GGCGACCGCGTCATGGGCCGCTGCCCAGGGGCCTTTTCCGAATACGTCCTGATGGATGTGCGCGAGGCCATGGTCATGCC
GGAGGGCCTGTCATGGGAGCAAGCTGCCAGCGTTCCCCTGACCTTTTTGGTGGTCTATGACATGCTGGTGCTGCAGGGGT
GTCTTGCCGCTGATGAATGGCTGCTGATCAATGGCGTGTCGTCGGGCGTCGGCGTTGCCGCGCTGCAAATGGCCAAGGCC
CTGGGGGCCAAGGTCATCGGCACCTCCGGCTCGACCGACAAGCTGGCTCGCCTTGATCCGCTGGGCCTGGATGTCGGCCT
GTGCACGCGCTCCGCTGATTTCTACGAGGCCGTCATGCAGGCCACCGGCGGCAAGGGCGTGAACCTGGTGGTGAACACCG
TGGGTGGCTCGGTGTTTGCCGAAAACATTCGCTGCGCGGCCTTTCAGGGGCGGCTGGCCACGGTCGGCTATGTCGATGGC
GTGCTCAAGGCCGATCTTGATCTTGAGGTCCTGCATGCCAGGCGACTGACGCTGTTCGGCGTGTCCAACAAGCTTCGCAC
GCCCGAGCAGCGCGCCTGGGCTGTCCCGGGTTTCGTGGCCGACGTGGTGCCCCTCCTTGCGGCGGGACGCATCAGGCCGC
TGGTGGATCGCGTCTTTCCGTTTGGAGAGCTCGCGGCCGCCAAGGCCCACATGGAATCCAACCAGCACCTGGGCAAGATC
GTGCTGTCCATGCCAGTTACACCCTGA

Upstream 100 bases:

>100_bases
CATGGAAAAACGCAAACCGGCTTTTACCGGGAAATAACCGGCGCCTGGCCCGGCCACGCCGACACACCCACCCACACCGA
CACCGAAAGAGAGCAAGCGA

Downstream 100 bases:

>100_bases
ACCGCGAACGCATACACGCACAGACGTATACACAAATCAAGGAGACATTCAGCATGAAAAAAGCAAACCTCATCGTGGCC
CTGGTCCTCTGCAGCTTCGG

Product: zinc-binding alcohol dehydrogenase

Products: NADP(+); Semiquinone. [C]

Alternate protein names: NA

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MQSYWMQMNADHATLELRDVPQPEPGAGQVLVRLHAASLNRGEFIAGHGLHGKPGSAKAVGMEGAGEVVGLGAGVRGLKA
GDRVMGRCPGAFSEYVLMDVREAMVMPEGLSWEQAASVPLTFLVVYDMLVLQGCLAADEWLLINGVSSGVGVAALQMAKA
LGAKVIGTSGSTDKLARLDPLGLDVGLCTRSADFYEAVMQATGGKGVNLVVNTVGGSVFAENIRCAAFQGRLATVGYVDG
VLKADLDLEVLHARRLTLFGVSNKLRTPEQRAWAVPGFVADVVPLLAAGRIRPLVDRVFPFGELAAAKAHMESNQHLGKI
VLSMPVTP

Sequences:

>Translated_328_residues
MQSYWMQMNADHATLELRDVPQPEPGAGQVLVRLHAASLNRGEFIAGHGLHGKPGSAKAVGMEGAGEVVGLGAGVRGLKA
GDRVMGRCPGAFSEYVLMDVREAMVMPEGLSWEQAASVPLTFLVVYDMLVLQGCLAADEWLLINGVSSGVGVAALQMAKA
LGAKVIGTSGSTDKLARLDPLGLDVGLCTRSADFYEAVMQATGGKGVNLVVNTVGGSVFAENIRCAAFQGRLATVGYVDG
VLKADLDLEVLHARRLTLFGVSNKLRTPEQRAWAVPGFVADVVPLLAAGRIRPLVDRVFPFGELAAAKAHMESNQHLGKI
VLSMPVTP
>Mature_328_residues
MQSYWMQMNADHATLELRDVPQPEPGAGQVLVRLHAASLNRGEFIAGHGLHGKPGSAKAVGMEGAGEVVGLGAGVRGLKA
GDRVMGRCPGAFSEYVLMDVREAMVMPEGLSWEQAASVPLTFLVVYDMLVLQGCLAADEWLLINGVSSGVGVAALQMAKA
LGAKVIGTSGSTDKLARLDPLGLDVGLCTRSADFYEAVMQATGGKGVNLVVNTVGGSVFAENIRCAAFQGRLATVGYVDG
VLKADLDLEVLHARRLTLFGVSNKLRTPEQRAWAVPGFVADVVPLLAAGRIRPLVDRVFPFGELAAAKAHMESNQHLGKI
VLSMPVTP

Specific function: Unknown

COG id: COG0604

COG function: function code CR; NADPH:quinone reductase and related Zn-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily [H]

Homologues:

Organism=Homo sapiens, GI22538446, Length=321, Percent_Identity=33.6448598130841, Blast_Score=179, Evalue=3e-45,
Organism=Homo sapiens, GI22538444, Length=321, Percent_Identity=33.6448598130841, Blast_Score=179, Evalue=3e-45,
Organism=Homo sapiens, GI18379349, Length=328, Percent_Identity=28.6585365853659, Blast_Score=113, Evalue=3e-25,
Organism=Homo sapiens, GI194239674, Length=301, Percent_Identity=24.9169435215947, Blast_Score=105, Evalue=7e-23,
Organism=Homo sapiens, GI13236495, Length=301, Percent_Identity=24.9169435215947, Blast_Score=105, Evalue=7e-23,
Organism=Homo sapiens, GI41872631, Length=325, Percent_Identity=29.8461538461538, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI194239676, Length=163, Percent_Identity=33.7423312883436, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI24308257, Length=325, Percent_Identity=24.9230769230769, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI28557745, Length=242, Percent_Identity=30.5785123966942, Blast_Score=82, Evalue=8e-16,
Organism=Escherichia coli, GI1790485, Length=306, Percent_Identity=29.0849673202614, Blast_Score=104, Evalue=9e-24,
Organism=Caenorhabditis elegans, GI17507255, Length=299, Percent_Identity=26.7558528428094, Blast_Score=88, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI212642053, Length=278, Percent_Identity=28.7769784172662, Blast_Score=82, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6324486, Length=343, Percent_Identity=25.3644314868805, Blast_Score=89, Evalue=7e-19,
Organism=Saccharomyces cerevisiae, GI6323961, Length=317, Percent_Identity=25.8675078864353, Blast_Score=87, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6319520, Length=322, Percent_Identity=22.360248447205, Blast_Score=80, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6319621, Length=305, Percent_Identity=25.2459016393443, Blast_Score=77, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6323729, Length=321, Percent_Identity=23.3644859813084, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24581345, Length=225, Percent_Identity=30.6666666666667, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI19920632, Length=251, Percent_Identity=29.0836653386454, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI221330659, Length=251, Percent_Identity=29.0836653386454, Blast_Score=91, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: 1.6.5.5 [C]

Molecular weight: Translated: 34495; Mature: 34495

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSYWMQMNADHATLELRDVPQPEPGAGQVLVRLHAASLNRGEFIAGHGLHGKPGSAKAV
CCCEEEEECCCCEEEEEECCCCCCCCCCEEEEEEEECCCCCCCEEECCCCCCCCCCCEEE
GMEGAGEVVGLGAGVRGLKAGDRVMGRCPGAFSEYVLMDVREAMVMPEGLSWEQAASVPL
CCCCCCCEEEECCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHCCCCCCHHHHCCCCH
TFLVVYDMLVLQGCLAADEWLLINGVSSGVGVAALQMAKALGAKVIGTSGSTDKLARLDP
HHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHCCEEEECCCCCCCHHCCCC
LGLDVGLCTRSADFYEAVMQATGGKGVNLVVNTVGGSVFAENIRCAAFQGRLATVGYVDG
CCCCHHHHCCCHHHHHHHHHHCCCCCEEEEEECCCCCEECCCCEEEEECCCEEEEHHHHH
VLKADLDLEVLHARRLTLFGVSNKLRTPEQRAWAVPGFVADVVPLLAAGRIRPLVDRVFP
HHHCCCCCEEHHHCEEEEEECCCCCCCCHHHHEECCHHHHHHHHHHHCCCHHHHHHHHCC
FGELAAAKAHMESNQHLGKIVLSMPVTP
CHHHHHHHHHHHCCCCCEEEEEECCCCC
>Mature Secondary Structure
MQSYWMQMNADHATLELRDVPQPEPGAGQVLVRLHAASLNRGEFIAGHGLHGKPGSAKAV
CCCEEEEECCCCEEEEEECCCCCCCCCCEEEEEEEECCCCCCCEEECCCCCCCCCCCEEE
GMEGAGEVVGLGAGVRGLKAGDRVMGRCPGAFSEYVLMDVREAMVMPEGLSWEQAASVPL
CCCCCCCEEEECCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHCCCCCCHHHHCCCCH
TFLVVYDMLVLQGCLAADEWLLINGVSSGVGVAALQMAKALGAKVIGTSGSTDKLARLDP
HHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHCCEEEECCCCCCCHHCCCC
LGLDVGLCTRSADFYEAVMQATGGKGVNLVVNTVGGSVFAENIRCAAFQGRLATVGYVDG
CCCCHHHHCCCHHHHHHHHHHCCCCCEEEEEECCCCCEECCCCEEEEECCCEEEEHHHHH
VLKADLDLEVLHARRLTLFGVSNKLRTPEQRAWAVPGFVADVVPLLAAGRIRPLVDRVFP
HHHCCCCCEEHHHCEEEEEECCCCCCCCHHHHEECCHHHHHHHHHHHCCCHHHHHHHHCC
FGELAAAKAHMESNQHLGKIVLSMPVTP
CHHHHHHHHHHHCCCCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.017 {9,10-phenanthrenequinone}} 0.0153 {2,6-dichlorophenolindophenol}} 0.013 {2,6-dichlorophenolindophenol}} 0.065 {1,4-benzoquinone}} 0.007 {NADPH}} 0.0069 {NADPH}} 0.26 {1,4-naphthoquinone}} 0.0024 {1,2-naphthoquinone}} [C]

Substrates: NADPH; Quinone [C]

Specific reaction: NADPH + Quinone = NADP(+) + Semiquinone. [C]

General reaction: Oxidation; Reduction [C]

Inhibitor: 2, 3-Dimercaptopropanol; 2, 5-Dichloro-3, 6-dihydroxy -1, 4-benzoquinone; 4-Hydroxy coumarin; 5, 5'-Dithiobis (2-nitrobenzoate); ADP; Cibacronblue3GA; Coumarin; Cu2+; Dicoumarol warfarin, coumarin; Dithiothreitol preincubation with 9, 10-phenanthrenequino

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]