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The map label for this gene is rsmD [H]

Identifier: 91787177

GI number: 91787177

Start: 1323852

End: 1324505

Strand: Direct

Name: rsmD [H]

Synonym: Bpro_1281

Alternate gene names: 91787177

Gene position: 1323852-1324505 (Clockwise)

Preceding gene: 91787176

Following gene: 91787178

Centisome position: 25.46

GC content: 63.76

Gene sequence:

>654_bases
ATGAAATACACCGCACTGGCAACTGAAAAGAAAACAGACAAGAAAGCGGAAAAAAAACCTGTCAGGTCGGGCAAGGTGGT
AGCGCGTAAAGCCAAACCTCCTGGCGAAATCCGCATCATTGGCGGGCAGTACAAACGCACCAAACTCCCGGTGCCCCACA
AGCCCGGCCTGCGTCCCACCCCCGATCGCGTGCGCGAAACCCTGTTTAACTGGCTGGGCCATGATCTGACCGGCTGGCAC
TGCGTCGATGTGTTTGCCGGTACCGGCGCACTGGGATTTGAAGCGGCCTCGCGGGGCGCGACCGAGGTGTTGCTGTGCGA
GCAGGACCCCGGGCTGGTGTTGCGGCTCAAGGCCCTGCAAACGCAATTGCAGGCCGGCATGGTGCGGATCGAGCGCGGCG
ACGGCGTGGGCCTGCTGCGGCGACTGCCCGCGGCCAGCATGCAGCTGGTGCTGCTGGACCCGCCGTTTGAGTCCGAACTG
TTTGAGCCCGCGCTGAAAGCCGCCGTGCAGGTGTTGGGGCCTACCGGCCTTGTCTACCTGGAGGCACCCCGGCACTGGCT
CGATGAGGAACTGCAGCCGCTGGGCCTGCGGGTACACCGCAGCGGCAAGGCGGGTGCGGTGCATTTCCATTTGCTGGTGC
GCATCGACAGTTGA

Upstream 100 bases:

>100_bases
CGCAGCAGATCGACAAGGTCACCCGGGCGGAGATCAAGGCGGCATTTGCCCGCAAATTACAACCCGACAAGATGATTACC
GTGATTCTGGGAGCCGCGCC

Downstream 100 bases:

>100_bases
GGCGCCGGCTGGCCGCCAAGGCAGGGCGGCTGCGGCCCTGTTTGGCTTGGGGTTACGCGCGCCCGAGGCCGGCGGGGCAT
AATTCCCCCATGCCCGCCAC

Product: hypothetical protein

Products: NA

Alternate protein names: 16S rRNA m2G966 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase [H]

Number of amino acids: Translated: 217; Mature: 217

Protein sequence:

>217_residues
MKYTALATEKKTDKKAEKKPVRSGKVVARKAKPPGEIRIIGGQYKRTKLPVPHKPGLRPTPDRVRETLFNWLGHDLTGWH
CVDVFAGTGALGFEAASRGATEVLLCEQDPGLVLRLKALQTQLQAGMVRIERGDGVGLLRRLPAASMQLVLLDPPFESEL
FEPALKAAVQVLGPTGLVYLEAPRHWLDEELQPLGLRVHRSGKAGAVHFHLLVRIDS

Sequences:

>Translated_217_residues
MKYTALATEKKTDKKAEKKPVRSGKVVARKAKPPGEIRIIGGQYKRTKLPVPHKPGLRPTPDRVRETLFNWLGHDLTGWH
CVDVFAGTGALGFEAASRGATEVLLCEQDPGLVLRLKALQTQLQAGMVRIERGDGVGLLRRLPAASMQLVLLDPPFESEL
FEPALKAAVQVLGPTGLVYLEAPRHWLDEELQPLGLRVHRSGKAGAVHFHLLVRIDS
>Mature_217_residues
MKYTALATEKKTDKKAEKKPVRSGKVVARKAKPPGEIRIIGGQYKRTKLPVPHKPGLRPTPDRVRETLFNWLGHDLTGWH
CVDVFAGTGALGFEAASRGATEVLLCEQDPGLVLRLKALQTQLQAGMVRIERGDGVGLLRRLPAASMQLVLLDPPFESEL
FEPALKAAVQVLGPTGLVYLEAPRHWLDEELQPLGLRVHRSGKAGAVHFHLLVRIDS

Specific function: Specifically methylates the guanosine in position 966 of 16S rRNA in the assembled 30S particle [H]

COG id: COG0742

COG function: function code L; N6-adenine-specific methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmD family [H]

Homologues:

Organism=Escherichia coli, GI1789875, Length=201, Percent_Identity=38.8059701492537, Blast_Score=119, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004398 [H]

Pfam domain/function: PF03602 Cons_hypoth95 [H]

EC number: =2.1.1.171 [H]

Molecular weight: Translated: 23864; Mature: 23864

Theoretical pI: Translated: 10.44; Mature: 10.44

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYTALATEKKTDKKAEKKPVRSGKVVARKAKPPGEIRIIGGQYKRTKLPVPHKPGLRPT
CCCCCCCCCCCHHHHHHHCCCCCCCEEEECCCCCCCEEEECCCCEECCCCCCCCCCCCCC
PDRVRETLFNWLGHDLTGWHCVDVFAGTGALGFEAASRGATEVLLCEQDPGLVLRLKALQ
HHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHCCCCEEEEEEECCCCEEEEHHHHH
TQLQAGMVRIERGDGVGLLRRLPAASMQLVLLDPPFESELFEPALKAAVQVLGPTGLVYL
HHHHHCEEEEECCCCHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCEEEE
EAPRHWLDEELQPLGLRVHRSGKAGAVHFHLLVRIDS
CCCHHHHHHHCCCCCEEEECCCCCCEEEEEEEEEECC
>Mature Secondary Structure
MKYTALATEKKTDKKAEKKPVRSGKVVARKAKPPGEIRIIGGQYKRTKLPVPHKPGLRPT
CCCCCCCCCCCHHHHHHHCCCCCCCEEEECCCCCCCEEEECCCCEECCCCCCCCCCCCCC
PDRVRETLFNWLGHDLTGWHCVDVFAGTGALGFEAASRGATEVLLCEQDPGLVLRLKALQ
HHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHCCCCEEEEEEECCCCEEEEHHHHH
TQLQAGMVRIERGDGVGLLRRLPAASMQLVLLDPPFESELFEPALKAAVQVLGPTGLVYL
HHHHHCEEEEECCCCHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCEEEE
EAPRHWLDEELQPLGLRVHRSGKAGAVHFHLLVRIDS
CCCHHHHHHHCCCCCEEEECCCCCCEEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]