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The map label for this gene is murD

Identifier: 91786971

GI number: 91786971

Start: 1104707

End: 1106470

Strand: Direct

Name: murD

Synonym: Bpro_1073

Alternate gene names: 91786971

Gene position: 1104707-1106470 (Clockwise)

Preceding gene: 91786970

Following gene: 91786972

Centisome position: 21.24

GC content: 64.97

Gene sequence:

>1764_bases
ATGAGGCATTTACAAGACCAAACCGTGTTGATCCTGGGCCTGGGTGCGTCCGGCCTGGCCATGGCGCGCTGGTGGGTCCG
CCATGGGGCATCGGTGACGGTTGCCGACACACGCGAAGCGCCTGCATTGCTCGCGACGCTGCGGCAGGAACTTCCCGCGG
TGAAGTTTGTGAGCGGCGCCTTTACGCCGGACCTCGTGCAGGGTAGCGCCGTGCGCGCGGTCTATCGCTCGCCAGGACTG
GCGCCTGCAGTGATCGCTCCTGTGGTTGATGCCGCGCGCGCCATGGGCTTGCCTGTCGGGGGCGAACTAGACCTGTTTTC
CCGGGCCCTGGCCGATCTCCGCGAGGTGTCGGCAACGGAGGTGGTGAAAGATCATCTCGTGCCTCCTGAATCGCCATTGT
CCGATGCATCCGATATATCCGATGCATCAGATGCAACGGATGCGGTGGACTCGCTTGAGGGCGAAGCCGCTTTGGCGGCT
GATGCCTCCGGCGATATCGAAGAGATGTCCGCCTCGGATGTTGTGGAAGGCGCGCCTGTCTCGGAGGATGCCGGCGAAGA
CGCGGCGTTGCCCGCTCTGCTTGCCCCCGCGCCGGCCGAACAGGCTGCTGGCTATAGGCCGGTGGTGTTGGCCATCACGG
GAACCAACGGCAAAACCACGGTAACTTCCCTGGTCGGCCAACTGGTGCAACGGGCCGGAAAAACCGTTGCCGTTGCAGGC
AATATCGGCCCGACGCTGCTCGATACGCTGTCTGCGCACCTTGACGCGGATACCCTGCCGCAGGTGTGGGTGCTGGAACT
GTCCAGCTTCCAGCTCGCGGACGCGCAGGACTTTGAGCCGACGGCTGCCGTGGTGTTGAATGTGACCCAGGATCATCTGG
ACTGGCACGGCGACATGGATGCTTACGTCGCGGCCAAGGCCCGGATATTTGGACGCAGCACGTTCATGCTGCTTAACCGT
GACGATCCCCGGGTGATGGAGATGCTTCCGACGCCCGTCAAGGTACGCCTGCGGCCTCCGCAGGTTCGCCCGCATTCGAC
TTTTGGTGCCGGGGAGCCACAACGACCCGGCGACTACGGCATCGAAACCGTCAATGGCATGGCGTGGCTGGTGCGCGCCG
CGCAAGCCGATGAAACCATCAAACGCCGCAAGGGTGAAGAAGTGGAGCTGCACATCCAGCGCCTGATGCCGCTGGATGCC
CTGCGCATTCGCGGCCGCCACAACGCCACCAATGCACTGGCCGCGCTCGGACTGGCCGTCGCGGCCGGTTGCAGTCTTGC
GCCCATGTTGCATGGCCTGCGCGAGTACCGGGGCGAGCCGCACCGCGTGGAATCCATCGCGGTGGTGAACGATGTCGAAT
ATTTCGACGACAGCAAGGGAACCAACGTCGGCGCCACCGCGGCAGCCCTGGCGGGTCTGGGGGCCGAACGCAAGCTGGTG
GTGATTCTGGGCGGCGAAGGCAAGGGGCAGGATTTTTCACCGCTGGCGGAACCCGTGTCACACCATGCCCGGGCCGTGGT
GCTGATAGGCAAGGACGCGCCCCTCATCCGGTCTGCGCTGCAGGCCAGCCAGGTGGCGCTGCTGGACGCCGCGTCGATGC
AGGAGGCGGTGAGCCTGGCGGCCGCGCAGGCCCATACCGGCGATGCCGTCCTGATGTCGCCCGCCTGCGCCAGTTTTGAC
ATGTTTGACAACTACGAACACCGCGCCCAGGTCTTTTGTGATGCGGTGCAGGCGCTGGCCCAGGAACAGGGGGTGGTGCT
GTGA

Upstream 100 bases:

>100_bases
GAAAAGCGGCTGGAAGGAGACCCAGGTGGTCGTCCGCTTCTGGATCATCACCATGCTGCTGTGCCTGGTGGGTCTCTCAA
CGTTGAAGCTCAGGTAAGGC

Downstream 100 bases:

>100_bases
CCGTGGCTGCACGCTTTTGGGGTTGGCTTTCCGGTCTCGGCGGCGTCGGCGCGGTCGCCGCGGATGTGCTGCCCGTTCGC
CTCGGGCGGCAGAGCCTGTC

Product: UDP-N-acetylmuramoylalanine--D-glutamate ligase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Number of amino acids: Translated: 587; Mature: 587

Protein sequence:

>587_residues
MRHLQDQTVLILGLGASGLAMARWWVRHGASVTVADTREAPALLATLRQELPAVKFVSGAFTPDLVQGSAVRAVYRSPGL
APAVIAPVVDAARAMGLPVGGELDLFSRALADLREVSATEVVKDHLVPPESPLSDASDISDASDATDAVDSLEGEAALAA
DASGDIEEMSASDVVEGAPVSEDAGEDAALPALLAPAPAEQAAGYRPVVLAITGTNGKTTVTSLVGQLVQRAGKTVAVAG
NIGPTLLDTLSAHLDADTLPQVWVLELSSFQLADAQDFEPTAAVVLNVTQDHLDWHGDMDAYVAAKARIFGRSTFMLLNR
DDPRVMEMLPTPVKVRLRPPQVRPHSTFGAGEPQRPGDYGIETVNGMAWLVRAAQADETIKRRKGEEVELHIQRLMPLDA
LRIRGRHNATNALAALGLAVAAGCSLAPMLHGLREYRGEPHRVESIAVVNDVEYFDDSKGTNVGATAAALAGLGAERKLV
VILGGEGKGQDFSPLAEPVSHHARAVVLIGKDAPLIRSALQASQVALLDAASMQEAVSLAAAQAHTGDAVLMSPACASFD
MFDNYEHRAQVFCDAVQALAQEQGVVL

Sequences:

>Translated_587_residues
MRHLQDQTVLILGLGASGLAMARWWVRHGASVTVADTREAPALLATLRQELPAVKFVSGAFTPDLVQGSAVRAVYRSPGL
APAVIAPVVDAARAMGLPVGGELDLFSRALADLREVSATEVVKDHLVPPESPLSDASDISDASDATDAVDSLEGEAALAA
DASGDIEEMSASDVVEGAPVSEDAGEDAALPALLAPAPAEQAAGYRPVVLAITGTNGKTTVTSLVGQLVQRAGKTVAVAG
NIGPTLLDTLSAHLDADTLPQVWVLELSSFQLADAQDFEPTAAVVLNVTQDHLDWHGDMDAYVAAKARIFGRSTFMLLNR
DDPRVMEMLPTPVKVRLRPPQVRPHSTFGAGEPQRPGDYGIETVNGMAWLVRAAQADETIKRRKGEEVELHIQRLMPLDA
LRIRGRHNATNALAALGLAVAAGCSLAPMLHGLREYRGEPHRVESIAVVNDVEYFDDSKGTNVGATAAALAGLGAERKLV
VILGGEGKGQDFSPLAEPVSHHARAVVLIGKDAPLIRSALQASQVALLDAASMQEAVSLAAAQAHTGDAVLMSPACASFD
MFDNYEHRAQVFCDAVQALAQEQGVVL
>Mature_587_residues
MRHLQDQTVLILGLGASGLAMARWWVRHGASVTVADTREAPALLATLRQELPAVKFVSGAFTPDLVQGSAVRAVYRSPGL
APAVIAPVVDAARAMGLPVGGELDLFSRALADLREVSATEVVKDHLVPPESPLSDASDISDASDATDAVDSLEGEAALAA
DASGDIEEMSASDVVEGAPVSEDAGEDAALPALLAPAPAEQAAGYRPVVLAITGTNGKTTVTSLVGQLVQRAGKTVAVAG
NIGPTLLDTLSAHLDADTLPQVWVLELSSFQLADAQDFEPTAAVVLNVTQDHLDWHGDMDAYVAAKARIFGRSTFMLLNR
DDPRVMEMLPTPVKVRLRPPQVRPHSTFGAGEPQRPGDYGIETVNGMAWLVRAAQADETIKRRKGEEVELHIQRLMPLDA
LRIRGRHNATNALAALGLAVAAGCSLAPMLHGLREYRGEPHRVESIAVVNDVEYFDDSKGTNVGATAAALAGLGAERKLV
VILGGEGKGQDFSPLAEPVSHHARAVVLIGKDAPLIRSALQASQVALLDAASMQEAVSLAAAQAHTGDAVLMSPACASFD
MFDNYEHRAQVFCDAVQALAQEQGVVL

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA)

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family

Homologues:

Organism=Escherichia coli, GI1786276, Length=377, Percent_Identity=39.2572944297082, Blast_Score=194, Evalue=1e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURD_POLSJ (Q12EL7)

Other databases:

- EMBL:   CP000316
- RefSeq:   YP_547923.1
- ProteinModelPortal:   Q12EL7
- SMR:   Q12EL7
- STRING:   Q12EL7
- GeneID:   4012060
- GenomeReviews:   CP000316_GR
- KEGG:   pol:Bpro_1073
- NMPDR:   fig|296591.1.peg.3332
- eggNOG:   COG0771
- HOGENOM:   HBG750024
- OMA:   ATRWCFE
- PhylomeDB:   Q12EL7
- BioCyc:   PSP296591:BPRO_1073-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00639
- InterPro:   IPR018109
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR01087

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.9

Molecular weight: Translated: 61626; Mature: 61626

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRHLQDQTVLILGLGASGLAMARWWVRHGASVTVADTREAPALLATLRQELPAVKFVSGA
CCCCCCCEEEEEEECCCHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHCCCEEEHHCC
FTPDLVQGSAVRAVYRSPGLAPAVIAPVVDAARAMGLPVGGELDLFSRALADLREVSATE
CCHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
VVKDHLVPPESPLSDASDISDASDATDAVDSLEGEAALAADASGDIEEMSASDVVEGAPV
HHHHHCCCCCCCCCCCHHCCCCCHHHHHHHHCCCCEEEECCCCCCHHHHCHHHHCCCCCC
SEDAGEDAALPALLAPAPAEQAAGYRPVVLAITGTNGKTTVTSLVGQLVQRAGKTVAVAG
CCCCCCCCCCCHHCCCCCHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCEEEEEC
NIGPTLLDTLSAHLDADTLPQVWVLELSSFQLADAQDFEPTAAVVLNVTQDHLDWHGDMD
CCCHHHHHHHHHHCCCCCCCCEEEEEECCCEECCCCCCCCCEEEEEEEHHHHCCCCCCCH
AYVAAKARIFGRSTFMLLNRDDPRVMEMLPTPVKVRLRPPQVRPHSTFGAGEPQRPGDYG
HHHHHHHHEECCEEEEEEECCCCHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCC
IETVNGMAWLVRAAQADETIKRRKGEEVELHIQRLMPLDALRIRGRHNATNALAALGLAV
CHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHCCCCCHHHHHHHHHHHH
AAGCSLAPMLHGLREYRGEPHRVESIAVVNDVEYFDDSKGTNVGATAAALAGLGAERKLV
HCCCHHHHHHHHHHHHCCCCCCEEEEEEEECHHHHCCCCCCCCCHHHHHHHCCCCCCEEE
VILGGEGKGQDFSPLAEPVSHHARAVVLIGKDAPLIRSALQASQVALLDAASMQEAVSLA
EEECCCCCCCCCCHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAQAHTGDAVLMSPACASFDMFDNYEHRAQVFCDAVQALAQEQGVVL
HHHCCCCCEEEECCCCCCCHHHCCHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRHLQDQTVLILGLGASGLAMARWWVRHGASVTVADTREAPALLATLRQELPAVKFVSGA
CCCCCCCEEEEEEECCCHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHCCCEEEHHCC
FTPDLVQGSAVRAVYRSPGLAPAVIAPVVDAARAMGLPVGGELDLFSRALADLREVSATE
CCHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
VVKDHLVPPESPLSDASDISDASDATDAVDSLEGEAALAADASGDIEEMSASDVVEGAPV
HHHHHCCCCCCCCCCCHHCCCCCHHHHHHHHCCCCEEEECCCCCCHHHHCHHHHCCCCCC
SEDAGEDAALPALLAPAPAEQAAGYRPVVLAITGTNGKTTVTSLVGQLVQRAGKTVAVAG
CCCCCCCCCCCHHCCCCCHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCEEEEEC
NIGPTLLDTLSAHLDADTLPQVWVLELSSFQLADAQDFEPTAAVVLNVTQDHLDWHGDMD
CCCHHHHHHHHHHCCCCCCCCEEEEEECCCEECCCCCCCCCEEEEEEEHHHHCCCCCCCH
AYVAAKARIFGRSTFMLLNRDDPRVMEMLPTPVKVRLRPPQVRPHSTFGAGEPQRPGDYG
HHHHHHHHEECCEEEEEEECCCCHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCC
IETVNGMAWLVRAAQADETIKRRKGEEVELHIQRLMPLDALRIRGRHNATNALAALGLAV
CHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHCCCCCHHHHHHHHHHHH
AAGCSLAPMLHGLREYRGEPHRVESIAVVNDVEYFDDSKGTNVGATAAALAGLGAERKLV
HCCCHHHHHHHHHHHHCCCCCCEEEEEEEECHHHHCCCCCCCCCHHHHHHHCCCCCCEEE
VILGGEGKGQDFSPLAEPVSHHARAVVLIGKDAPLIRSALQASQVALLDAASMQEAVSLA
EEECCCCCCCCCCHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAQAHTGDAVLMSPACASFDMFDNYEHRAQVFCDAVQALAQEQGVVL
HHHCCCCCEEEECCCCCCCHHHCCHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA