Click here to switch to the map view.

The map label for this gene is rumA [H]

Identifier: 89256815

GI number: 89256815

Start: 1461047

End: 1462396

Strand: Reverse

Name: rumA [H]

Synonym: FTL_1531

Alternate gene names: 89256815

Gene position: 1462396-1461047 (Counterclockwise)

Preceding gene: 89256816

Following gene: 89256814

Centisome position: 77.13

GC content: 33.11

Gene sequence:

>1350_bases
ATGGGAAGATCTAGATATCATAATAAGCTAAAAGAGGGAATCTTTGAGGCTGAAATTACAGCATTAAGTCATGATGGTAG
AGGTATTGCAAAAGTTGATGGTAAAACTACTTTTATACCTTTCACATTACCTGGCGAAGTAGTTAAATTTGAATATACAT
TCACAAAAGCAAAATTCGATGAAGCAAAAGTAGTTGAGTATGTTAAAAAATCATTAAATAGAGTTAATCCGCCTTGTGAC
CATTTTCAAATATGTGGAGGTTGTAGCTTACAACATATGTCTACAGATGCACAGATCGAGCATAAGCAACAAACTTTAAT
TAATCAATTAAAATATATTGGTAATGGTGTTGAGCCAGAGAATATTTTACCACCACTACGCACGAGTAATACTGAAGGAT
ATCGTAATAAAGCACGTTTGGGTGTTAGGTATGTCAGTAAAAAAGGTAAAATTCTTGTTGGCTTTAGAGAGCGTAATGGT
AGGTTCTTGGCAGATATAGATAAGTGTATCGTTTTAAATCCCCTTGTCGGTGACAAAATAACAGAAATATCTTCATTTAT
AGAGACGTTATCTATATATCAACATATAGCACAATTAGAAATAGCAATAGATGATACAAGACCTGCTATGATTGTGCGTC
ATCTAGAACCATTTACAAATGAAGACTTAGAAAAGCTAAGATCATTTGCACAAGAAAATAATTATTGGATATATTTACAA
TCAAAAGGTCCAGATACTATTTTTAGATTATACCCACAAGGTGATGTAGAGCCTAAAAAGCTAAGCTATCAGCCTGCTGC
TGGAATAGATATTGGTTTTGAACCTAATGATTTTACTCAGGTTAATAATGATATCAATAAAAAAATGATTAAAAGAGCGA
TTGAGCTTTTAGATATATCAGAAAATGATTCTATTATTGACTTATTCTGTGGTTTAGGTAACTTTACGCTGCCAATTTCT
CAGCATGCAAAAACTGTAATTGGCGTTGAGGGTGAGCCAACAATGGTCAAAAGAGCCAAAGAAACTGCTGATAATAATAA
CATAACAAATGTTAATTTTTATGCGGCAAATCTTTTTGAGAGTTTTGAGGATAAAGAGTGGTTTAATAATTTTGAATATA
ACAAGATGCTTTTAGATCCGCCTCGAGCAGGCGCACAAGAAGTTTGTAACAATATTGAAAAATTTAATGTTAAGCGCATA
GTTTATGTTTCATGTGATACTGCTGCTTTAGCACGTGATGCTGGAATTTTGGTCAATACCAAAGGTTATAAACTAATAAG
TGCTGGTGTAATGGATATGTTCCCTCATACAATGCATGTTGAGTCAATAGCTGTTTTTGAGAAGATCTAG

Upstream 100 bases:

>100_bases
AATCTAAAATAGTAAAAGCTAAGACTAAGTTTTGTGATTAAGTTGTATTTATGATTTGAATTAAATACAATTGTCGTAAT
ATTTTTTTGTTTAATTATTT

Downstream 100 bases:

>100_bases
GAGACATTTATGTACATAGGTTTAGATATTAGCGGTAGTAATATCTCTGCTGGTATTTTTGATGAGCAAAAGAACCTTAT
TAAAACTGCAAAGGTTAAAT

Product: 23S rRNA 5-methyluridine methyltransferase

Products: NA

Alternate protein names: 23S rRNA(M-5-U1939)-methyltransferase [H]

Number of amino acids: Translated: 449; Mature: 448

Protein sequence:

>449_residues
MGRSRYHNKLKEGIFEAEITALSHDGRGIAKVDGKTTFIPFTLPGEVVKFEYTFTKAKFDEAKVVEYVKKSLNRVNPPCD
HFQICGGCSLQHMSTDAQIEHKQQTLINQLKYIGNGVEPENILPPLRTSNTEGYRNKARLGVRYVSKKGKILVGFRERNG
RFLADIDKCIVLNPLVGDKITEISSFIETLSIYQHIAQLEIAIDDTRPAMIVRHLEPFTNEDLEKLRSFAQENNYWIYLQ
SKGPDTIFRLYPQGDVEPKKLSYQPAAGIDIGFEPNDFTQVNNDINKKMIKRAIELLDISENDSIIDLFCGLGNFTLPIS
QHAKTVIGVEGEPTMVKRAKETADNNNITNVNFYAANLFESFEDKEWFNNFEYNKMLLDPPRAGAQEVCNNIEKFNVKRI
VYVSCDTAALARDAGILVNTKGYKLISAGVMDMFPHTMHVESIAVFEKI

Sequences:

>Translated_449_residues
MGRSRYHNKLKEGIFEAEITALSHDGRGIAKVDGKTTFIPFTLPGEVVKFEYTFTKAKFDEAKVVEYVKKSLNRVNPPCD
HFQICGGCSLQHMSTDAQIEHKQQTLINQLKYIGNGVEPENILPPLRTSNTEGYRNKARLGVRYVSKKGKILVGFRERNG
RFLADIDKCIVLNPLVGDKITEISSFIETLSIYQHIAQLEIAIDDTRPAMIVRHLEPFTNEDLEKLRSFAQENNYWIYLQ
SKGPDTIFRLYPQGDVEPKKLSYQPAAGIDIGFEPNDFTQVNNDINKKMIKRAIELLDISENDSIIDLFCGLGNFTLPIS
QHAKTVIGVEGEPTMVKRAKETADNNNITNVNFYAANLFESFEDKEWFNNFEYNKMLLDPPRAGAQEVCNNIEKFNVKRI
VYVSCDTAALARDAGILVNTKGYKLISAGVMDMFPHTMHVESIAVFEKI
>Mature_448_residues
GRSRYHNKLKEGIFEAEITALSHDGRGIAKVDGKTTFIPFTLPGEVVKFEYTFTKAKFDEAKVVEYVKKSLNRVNPPCDH
FQICGGCSLQHMSTDAQIEHKQQTLINQLKYIGNGVEPENILPPLRTSNTEGYRNKARLGVRYVSKKGKILVGFRERNGR
FLADIDKCIVLNPLVGDKITEISSFIETLSIYQHIAQLEIAIDDTRPAMIVRHLEPFTNEDLEKLRSFAQENNYWIYLQS
KGPDTIFRLYPQGDVEPKKLSYQPAAGIDIGFEPNDFTQVNNDINKKMIKRAIELLDISENDSIIDLFCGLGNFTLPISQ
HAKTVIGVEGEPTMVKRAKETADNNNITNVNFYAANLFESFEDKEWFNNFEYNKMLLDPPRAGAQEVCNNIEKFNVKRIV
YVSCDTAALARDAGILVNTKGYKLISAGVMDMFPHTMHVESIAVFEKI

Specific function: Catalyzes the formation of 5-methyl-uridine at position 1939 (M-5-U1939) in 23S rRNA [H]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI34222389, Length=191, Percent_Identity=27.7486910994764, Blast_Score=83, Evalue=4e-16,
Organism=Homo sapiens, GI51173878, Length=191, Percent_Identity=27.7486910994764, Blast_Score=83, Evalue=4e-16,
Organism=Escherichia coli, GI1789148, Length=436, Percent_Identity=36.0091743119266, Blast_Score=296, Evalue=2e-81,
Organism=Escherichia coli, GI1787083, Length=184, Percent_Identity=24.4565217391304, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1790403, Length=235, Percent_Identity=26.8085106382979, Blast_Score=72, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6322909, Length=431, Percent_Identity=25.0580046403712, Blast_Score=99, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24666579, Length=197, Percent_Identity=31.9796954314721, Blast_Score=95, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR002792
- InterPro:   IPR010280 [H]

Pfam domain/function: PF01938 TRAM; PF05958 tRNA_U5-meth_tr [H]

EC number: =2.1.1.190 [H]

Molecular weight: Translated: 50864; Mature: 50733

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS50926 TRAM ; PS01231 TRMA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRSRYHNKLKEGIFEAEITALSHDGRGIAKVDGKTTFIPFTLPGEVVKFEYTFTKAKFD
CCCHHHHHHHHHCHHHHHEEEEECCCCEEEEECCCEEEEEEECCCCEEEEEEEEECCCCC
EAKVVEYVKKSLNRVNPPCDHFQICGGCSLQHMSTDAQIEHKQQTLINQLKYIGNGVEPE
HHHHHHHHHHHHHHCCCCCCCEEECCCCCEECCCCCCHHHHHHHHHHHHHHHHCCCCCCH
NILPPLRTSNTEGYRNKARLGVRYVSKKGKILVGFRERNGRFLADIDKCIVLNPLVGDKI
HCCCCCCCCCCCCCCHHHHHHHEEECCCCCEEEEEECCCCCEEEECCCEEEECCCCCCHH
TEISSFIETLSIYQHIAQLEIAIDDTRPAMIVRHLEPFTNEDLEKLRSFAQENNYWIYLQ
HHHHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHCCCCCCHHHHHHHHHHHCCCEEEEEE
SKGPDTIFRLYPQGDVEPKKLSYQPAAGIDIGFEPNDFTQVNNDINKKMIKRAIELLDIS
CCCCCCEEEECCCCCCCHHHCCCCCCCCEECCCCCCCHHHHCCHHHHHHHHHHHHHHCCC
ENDSIIDLFCGLGNFTLPISQHAKTVIGVEGEPTMVKRAKETADNNNITNVNFYAANLFE
CCCCEEEEEECCCCCEEECCCCCCEEEECCCCCHHHHHHHHHCCCCCCEEEEEEHHHHHH
SFEDKEWFNNFEYNKMLLDPPRAGAQEVCNNIEKFNVKRIVYVSCDTAALARDAGILVNT
HHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHCCCEEEEEEECCHHHHHCCCCEEEEC
KGYKLISAGVMDMFPHTMHVESIAVFEKI
CCCEEEECCHHHHCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
GRSRYHNKLKEGIFEAEITALSHDGRGIAKVDGKTTFIPFTLPGEVVKFEYTFTKAKFD
CCHHHHHHHHHCHHHHHEEEEECCCCEEEEECCCEEEEEEECCCCEEEEEEEEECCCCC
EAKVVEYVKKSLNRVNPPCDHFQICGGCSLQHMSTDAQIEHKQQTLINQLKYIGNGVEPE
HHHHHHHHHHHHHHCCCCCCCEEECCCCCEECCCCCCHHHHHHHHHHHHHHHHCCCCCCH
NILPPLRTSNTEGYRNKARLGVRYVSKKGKILVGFRERNGRFLADIDKCIVLNPLVGDKI
HCCCCCCCCCCCCCCHHHHHHHEEECCCCCEEEEEECCCCCEEEECCCEEEECCCCCCHH
TEISSFIETLSIYQHIAQLEIAIDDTRPAMIVRHLEPFTNEDLEKLRSFAQENNYWIYLQ
HHHHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHCCCCCCHHHHHHHHHHHCCCEEEEEE
SKGPDTIFRLYPQGDVEPKKLSYQPAAGIDIGFEPNDFTQVNNDINKKMIKRAIELLDIS
CCCCCCEEEECCCCCCCHHHCCCCCCCCEECCCCCCCHHHHCCHHHHHHHHHHHHHHCCC
ENDSIIDLFCGLGNFTLPISQHAKTVIGVEGEPTMVKRAKETADNNNITNVNFYAANLFE
CCCCEEEEEECCCCCEEECCCCCCEEEECCCCCHHHHHHHHHCCCCCCEEEEEEHHHHHH
SFEDKEWFNNFEYNKMLLDPPRAGAQEVCNNIEKFNVKRIVYVSCDTAALARDAGILVNT
HHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHCCCEEEEEEECCHHHHHCCCCEEEEC
KGYKLISAGVMDMFPHTMHVESIAVFEKI
CCCEEEECCHHHHCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA