Click here to switch to the map view.

The map label for this gene is thrS [H]

Identifier: 89256700

GI number: 89256700

Start: 1333850

End: 1335754

Strand: Reverse

Name: thrS [H]

Synonym: FTL_1407

Alternate gene names: 89256700

Gene position: 1335754-1333850 (Counterclockwise)

Preceding gene: 89256702

Following gene: 89256699

Centisome position: 70.45

GC content: 36.27

Gene sequence:

>1905_bases
ATGATAAATATCAGATTTCCAGATGGTTCGATAAGAGAATTTGAAGCCGGTGTTAATTCTCTAGATGTTGCTAAATCAAT
ATCTCCAAGCTTAGCTAAAGCTACAATGGCTGCGTATATTGATGACCAGCTCAAAGATGCAAAAGATGCGATTAACAGTA
ACTGTGAATTAAGATTGATAACAGTAAAAGATCCAGAGGGGCTCGAAATTCTAAGACATTCTTGCGCTCATCTTTTAGCT
CATGCTGTCAAAGAGTTGTATCCAAATACTGAAGTAACTATAGGTCCGGTAGTTGATAATGGCTTTTATTATGATTTTTC
TTTCAAAGAGTCTATAGGTGAAGCAGATTTACCTACTATCGAAAAGAAAATGAAAGAATTAGCAAAAAAGTCTGCACCTG
TTAGTTATAGAGTGGTTCCTAAAGCTGAAGCAATTGAGTTTTTTAAGGCTCAAGGTGAGAACTATAAGGTTGAGATTATT
GATAGCATTGCTGATGAGCAGATGAAAATATATACCCAAGATAATTTTAGTGACTTGTGTAGAGGTCCTCATATTCCAAA
TACATCAGTATTAAAAGCATTTAAGTTGACAAAACTAGCAGGAGCTTATTGGCGAGGTAACTCTGATAATGAAATGCTAA
CTAGAATCTATGGCACATGCTGGGCAACTAAAGAAGATTTAGAGCAATATTTGAATATGCTTGAAGAGGCTGAAAAGCGT
GATCATAGAAAAATTGGTAAAGTTCTTGATCTATTTCATTTTCAAGAAGACTCACCAGGCATAGCTTTTTGGCATGATAA
TGGTGTGAGAATATGGCGTCAAGTAGAAGATTATATGCGAGCTTCTAATAATAAATATGGCTGTAGTGAGATTAGAACCC
CACTTATTGCAGATTTTAGTTTATGGCAAAAATCTGGTCACGCATCTAAGTATGCTGAGAATATGTTTGCAACAAAGTCT
GAGAACAGGGATTTTGCTATCAGACCAATGAACTGTCCAACTTGTGTACAGGTTTATAATACAAAGCTACATAGTTATAG
AGATCTACCTATAAGGATGGCTGAGTTCGGTATAGTACATAGGAATGAACCATCTGGATCGCTACACGGGTTATTAAGAG
TACGTAGCTTTACTCAAGATGATGGGCATATTTTCTGTACCCCTGAGCAGGTTGAAGAAGAAGTGATTTTGATGGTACAG
CAGTGTTTTGAAGTATACAAAGATTTTGGTTTTAATGATTTTGCGGTCAAGATTGCACTTAGACCTGAAAATAGAATCGG
AGATGATGAAACTTGGGATAAATCTGAACAGATTCTCAAAAATGCTCTTGACGCTAATAATGTTAGTTATGAACTATTTC
CAGGTGAGGGTGCATTTTATGGTCCTAAGATTGAGTTTCATCTCAAAGATGCAATTGGTAGAAGCTGGCAGTGTGGCACT
ATACAACTAGATTTTTCAATGCCACAAAGGCTAGGAGCTACATATATTGACAAGAATGGCGAGAAACAGGTGTCAGTTAT
GCTTCACAGAGCTATCGTTGGTTCTTTGGAGAGATTTATAGGTATGCTTATTGAGCATTATGCTGGAAATTTACCATTGT
GGTTAGCTCCAGTCCAAGTTGCTGTGATGGGAATTAGTAATAATCAGGATGACTACTGTAAAGAAGTATTTATAATGCTT
GAAAAAAATGGTATCCGCGCTAAATTAGACTTGAGAAATGAGAAAATAGGGTTTAAAATACGTGAGCATACTCTTTTGCG
TGTACCATACCTTGTTATCCTTGGTAAAAACGAGCAAGAGCAAAAGATTATCACAATAAGAAAGCATAGCGGCGAAGATC
TAGGACAGATGTCTGTAGATGATTTTTGTGCTTTTTTAGATAAGCAAATACAGGCTAAAGAATAA

Upstream 100 bases:

>100_bases
TTAAGTTTATTGATAAATAATTTACATAATTATAGCCATACAATATAATATATACTCTATGTAATATTTCATTAACAATA
TAATAGATTAAGTACTGCAA

Downstream 100 bases:

>100_bases
TAATTTCTGAGGAGGAAAAAGATTATTAAAGCGGATAAAAAAGCACCTATAAATGAGCAAATTAAAGCAAAAGAGGTGCG
TCTAGTTGGTGTTGATGGTC

Product: threonyl-tRNA synthetase

Products: NA

Alternate protein names: Threonine--tRNA ligase; ThrRS [H]

Number of amino acids: Translated: 634; Mature: 634

Protein sequence:

>634_residues
MINIRFPDGSIREFEAGVNSLDVAKSISPSLAKATMAAYIDDQLKDAKDAINSNCELRLITVKDPEGLEILRHSCAHLLA
HAVKELYPNTEVTIGPVVDNGFYYDFSFKESIGEADLPTIEKKMKELAKKSAPVSYRVVPKAEAIEFFKAQGENYKVEII
DSIADEQMKIYTQDNFSDLCRGPHIPNTSVLKAFKLTKLAGAYWRGNSDNEMLTRIYGTCWATKEDLEQYLNMLEEAEKR
DHRKIGKVLDLFHFQEDSPGIAFWHDNGVRIWRQVEDYMRASNNKYGCSEIRTPLIADFSLWQKSGHASKYAENMFATKS
ENRDFAIRPMNCPTCVQVYNTKLHSYRDLPIRMAEFGIVHRNEPSGSLHGLLRVRSFTQDDGHIFCTPEQVEEEVILMVQ
QCFEVYKDFGFNDFAVKIALRPENRIGDDETWDKSEQILKNALDANNVSYELFPGEGAFYGPKIEFHLKDAIGRSWQCGT
IQLDFSMPQRLGATYIDKNGEKQVSVMLHRAIVGSLERFIGMLIEHYAGNLPLWLAPVQVAVMGISNNQDDYCKEVFIML
EKNGIRAKLDLRNEKIGFKIREHTLLRVPYLVILGKNEQEQKIITIRKHSGEDLGQMSVDDFCAFLDKQIQAKE

Sequences:

>Translated_634_residues
MINIRFPDGSIREFEAGVNSLDVAKSISPSLAKATMAAYIDDQLKDAKDAINSNCELRLITVKDPEGLEILRHSCAHLLA
HAVKELYPNTEVTIGPVVDNGFYYDFSFKESIGEADLPTIEKKMKELAKKSAPVSYRVVPKAEAIEFFKAQGENYKVEII
DSIADEQMKIYTQDNFSDLCRGPHIPNTSVLKAFKLTKLAGAYWRGNSDNEMLTRIYGTCWATKEDLEQYLNMLEEAEKR
DHRKIGKVLDLFHFQEDSPGIAFWHDNGVRIWRQVEDYMRASNNKYGCSEIRTPLIADFSLWQKSGHASKYAENMFATKS
ENRDFAIRPMNCPTCVQVYNTKLHSYRDLPIRMAEFGIVHRNEPSGSLHGLLRVRSFTQDDGHIFCTPEQVEEEVILMVQ
QCFEVYKDFGFNDFAVKIALRPENRIGDDETWDKSEQILKNALDANNVSYELFPGEGAFYGPKIEFHLKDAIGRSWQCGT
IQLDFSMPQRLGATYIDKNGEKQVSVMLHRAIVGSLERFIGMLIEHYAGNLPLWLAPVQVAVMGISNNQDDYCKEVFIML
EKNGIRAKLDLRNEKIGFKIREHTLLRVPYLVILGKNEQEQKIITIRKHSGEDLGQMSVDDFCAFLDKQIQAKE
>Mature_634_residues
MINIRFPDGSIREFEAGVNSLDVAKSISPSLAKATMAAYIDDQLKDAKDAINSNCELRLITVKDPEGLEILRHSCAHLLA
HAVKELYPNTEVTIGPVVDNGFYYDFSFKESIGEADLPTIEKKMKELAKKSAPVSYRVVPKAEAIEFFKAQGENYKVEII
DSIADEQMKIYTQDNFSDLCRGPHIPNTSVLKAFKLTKLAGAYWRGNSDNEMLTRIYGTCWATKEDLEQYLNMLEEAEKR
DHRKIGKVLDLFHFQEDSPGIAFWHDNGVRIWRQVEDYMRASNNKYGCSEIRTPLIADFSLWQKSGHASKYAENMFATKS
ENRDFAIRPMNCPTCVQVYNTKLHSYRDLPIRMAEFGIVHRNEPSGSLHGLLRVRSFTQDDGHIFCTPEQVEEEVILMVQ
QCFEVYKDFGFNDFAVKIALRPENRIGDDETWDKSEQILKNALDANNVSYELFPGEGAFYGPKIEFHLKDAIGRSWQCGT
IQLDFSMPQRLGATYIDKNGEKQVSVMLHRAIVGSLERFIGMLIEHYAGNLPLWLAPVQVAVMGISNNQDDYCKEVFIML
EKNGIRAKLDLRNEKIGFKIREHTLLRVPYLVILGKNEQEQKIITIRKHSGEDLGQMSVDDFCAFLDKQIQAKE

Specific function: Thrs Is Also A Translational Repressor Protein, It Controls The Translation Of Its Own Gene By Binding To Its mRNA. [C]

COG id: COG0441

COG function: function code J; Threonyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI38570111, Length=624, Percent_Identity=37.0192307692308, Blast_Score=428, Evalue=1e-120,
Organism=Homo sapiens, GI38202255, Length=625, Percent_Identity=36.48, Blast_Score=424, Evalue=1e-118,
Organism=Homo sapiens, GI20070344, Length=642, Percent_Identity=34.4236760124611, Blast_Score=393, Evalue=1e-109,
Organism=Escherichia coli, GI1788013, Length=635, Percent_Identity=52.9133858267717, Blast_Score=721, Evalue=0.0,
Organism=Escherichia coli, GI1786392, Length=161, Percent_Identity=26.7080745341615, Blast_Score=68, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI71984184, Length=628, Percent_Identity=36.624203821656, Blast_Score=442, Evalue=1e-124,
Organism=Caenorhabditis elegans, GI71984192, Length=628, Percent_Identity=36.624203821656, Blast_Score=442, Evalue=1e-124,
Organism=Saccharomyces cerevisiae, GI6322112, Length=627, Percent_Identity=36.3636363636364, Blast_Score=423, Evalue=1e-119,
Organism=Saccharomyces cerevisiae, GI6322654, Length=389, Percent_Identity=36.5038560411311, Blast_Score=269, Evalue=1e-72,
Organism=Drosophila melanogaster, GI24583839, Length=631, Percent_Identity=36.608557844691, Blast_Score=452, Evalue=1e-127,
Organism=Drosophila melanogaster, GI24583842, Length=631, Percent_Identity=36.608557844691, Blast_Score=451, Evalue=1e-127,
Organism=Drosophila melanogaster, GI28574141, Length=631, Percent_Identity=36.608557844691, Blast_Score=451, Evalue=1e-127,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR012675
- InterPro:   IPR004095
- InterPro:   IPR012676
- InterPro:   IPR002320
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF02824 TGS; PF00587 tRNA-synt_2b; PF07973 tRNA_SAD [H]

EC number: =6.1.1.3 [H]

Molecular weight: Translated: 72382; Mature: 72382

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINIRFPDGSIREFEAGVNSLDVAKSISPSLAKATMAAYIDDQLKDAKDAINSNCELRLI
CEEEECCCCCHHHHHCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
TVKDPEGLEILRHSCAHLLAHAVKELYPNTEVTIGPVVDNGFYYDFSFKESIGEADLPTI
EECCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEEEEEHHHCCCCCCCHHH
EKKMKELAKKSAPVSYRVVPKAEAIEFFKAQGENYKVEIIDSIADEQMKIYTQDNFSDLC
HHHHHHHHHHCCCCEEEECCCHHHHHHHHHCCCCEEEEEEEHHCCCCEEEEECCCHHHHH
RGPHIPNTSVLKAFKLTKLAGAYWRGNSDNEMLTRIYGTCWATKEDLEQYLNMLEEAEKR
CCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
DHRKIGKVLDLFHFQEDSPGIAFWHDNGVRIWRQVEDYMRASNNKYGCSEIRTPLIADFS
HHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCHHHHCCCHHHHHH
LWQKSGHASKYAENMFATKSENRDFAIRPMNCPTCVQVYNTKLHSYRDLPIRMAEFGIVH
HHHCCCCHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHCCCCCCEEHHHCCEEE
RNEPSGSLHGLLRVRSFTQDDGHIFCTPEQVEEEVILMVQQCFEVYKDFGFNDFAVKIAL
CCCCCCHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEE
RPENRIGDDETWDKSEQILKNALDANNVSYELFPGEGAFYGPKIEFHLKDAIGRSWQCGT
CCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCEECCEEEEEEHHHCCCCCCCCE
IQLDFSMPQRLGATYIDKNGEKQVSVMLHRAIVGSLERFIGMLIEHYAGNLPLWLAPVQV
EEECCCCHHHCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECEEE
AVMGISNNQDDYCKEVFIMLEKNGIRAKLDLRNEKIGFKIREHTLLRVPYLVILGKNEQE
EEEECCCCCHHHHHHHHHEEECCCCEEEEECCCCCCCEEEECCEEEECCEEEEECCCCCC
QKIITIRKHSGEDLGQMSVDDFCAFLDKQIQAKE
CEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MINIRFPDGSIREFEAGVNSLDVAKSISPSLAKATMAAYIDDQLKDAKDAINSNCELRLI
CEEEECCCCCHHHHHCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
TVKDPEGLEILRHSCAHLLAHAVKELYPNTEVTIGPVVDNGFYYDFSFKESIGEADLPTI
EECCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEEEEEHHHCCCCCCCHHH
EKKMKELAKKSAPVSYRVVPKAEAIEFFKAQGENYKVEIIDSIADEQMKIYTQDNFSDLC
HHHHHHHHHHCCCCEEEECCCHHHHHHHHHCCCCEEEEEEEHHCCCCEEEEECCCHHHHH
RGPHIPNTSVLKAFKLTKLAGAYWRGNSDNEMLTRIYGTCWATKEDLEQYLNMLEEAEKR
CCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
DHRKIGKVLDLFHFQEDSPGIAFWHDNGVRIWRQVEDYMRASNNKYGCSEIRTPLIADFS
HHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCHHHHCCCHHHHHH
LWQKSGHASKYAENMFATKSENRDFAIRPMNCPTCVQVYNTKLHSYRDLPIRMAEFGIVH
HHHCCCCHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHCCCCCCEEHHHCCEEE
RNEPSGSLHGLLRVRSFTQDDGHIFCTPEQVEEEVILMVQQCFEVYKDFGFNDFAVKIAL
CCCCCCHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEE
RPENRIGDDETWDKSEQILKNALDANNVSYELFPGEGAFYGPKIEFHLKDAIGRSWQCGT
CCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCEECCEEEEEEHHHCCCCCCCCE
IQLDFSMPQRLGATYIDKNGEKQVSVMLHRAIVGSLERFIGMLIEHYAGNLPLWLAPVQV
EEECCCCHHHCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECEEE
AVMGISNNQDDYCKEVFIMLEKNGIRAKLDLRNEKIGFKIREHTLLRVPYLVILGKNEQE
EEEECCCCCHHHHHHHHHEEECCCCEEEEECCCCCCCEEEECCEEEECCEEEEECCCCCC
QKIITIRKHSGEDLGQMSVDDFCAFLDKQIQAKE
CEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA