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The map label for this gene is gcvH [H]

Identifier: 89256702

GI number: 89256702

Start: 1338357

End: 1338737

Strand: Reverse

Name: gcvH [H]

Synonym: FTL_1409

Alternate gene names: 89256702

Gene position: 1338737-1338357 (Counterclockwise)

Preceding gene: 89256706

Following gene: 89256700

Centisome position: 70.61

GC content: 31.23

Gene sequence:

>381_bases
ATGGCTGAAATAAATCAAAATTACTTATATACAGATTCGAATTTATGGGTTGAGGTCAAAGGTAATATTGCAAGAGTTGG
TATAGATGATTACTCCCAAGATGAATTTGGAGAAATTGTCTATGTTGATTTGCCTAAACTTGAACAAGAGTATGCCAAAG
ATGATGAGATTTGTGTTATAGAATCTGTAAAGACCGCATCTGATATTTATACACCTATATCTGGGAAAGTTGTAAAAATA
AATCAACAATTATTAGATAATCCAAAGCTTATAAACGAGTCTTGTTATGATAATGCTTGGCTATTTGAAATTGAAATAGC
TGATCTAGATGAGCTTAAGAGCTTAATAAATCCTCAAGACTATCAAAGCTATGTTAGCTAG

Upstream 100 bases:

>100_bases
TAGATTATCAACTTTGTTTTACAAATTAATTTTAAAGGATTTTTATGTTATTATCATCTATTAGCTAAAATAAAAATAGG
GTTTTAATCCAAGAGGAATT

Downstream 100 bases:

>100_bases
AGTTTTATTAATTCATAGCTTAATTTTTTTATCATCATCTGCTTTTGCTCTAGAGCAATATCTTAATTATTGTGAGATAT
ATAATCCAAAGAAAGGTATC

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 126; Mature: 125

Protein sequence:

>126_residues
MAEINQNYLYTDSNLWVEVKGNIARVGIDDYSQDEFGEIVYVDLPKLEQEYAKDDEICVIESVKTASDIYTPISGKVVKI
NQQLLDNPKLINESCYDNAWLFEIEIADLDELKSLINPQDYQSYVS

Sequences:

>Translated_126_residues
MAEINQNYLYTDSNLWVEVKGNIARVGIDDYSQDEFGEIVYVDLPKLEQEYAKDDEICVIESVKTASDIYTPISGKVVKI
NQQLLDNPKLINESCYDNAWLFEIEIADLDELKSLINPQDYQSYVS
>Mature_125_residues
AEINQNYLYTDSNLWVEVKGNIARVGIDDYSQDEFGEIVYVDLPKLEQEYAKDDEICVIESVKTASDIYTPISGKVVKIN
QQLLDNPKLINESCYDNAWLFEIEIADLDELKSLINPQDYQSYVS

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein [H]

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI49574537, Length=116, Percent_Identity=37.9310344827586, Blast_Score=108, Evalue=7e-25,
Organism=Homo sapiens, GI89057342, Length=116, Percent_Identity=37.0689655172414, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI1789271, Length=124, Percent_Identity=35.4838709677419, Blast_Score=95, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17507493, Length=120, Percent_Identity=37.5, Blast_Score=102, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI17551294, Length=118, Percent_Identity=37.2881355932203, Blast_Score=100, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6319272, Length=121, Percent_Identity=33.0578512396694, Blast_Score=79, Evalue=2e-16,
Organism=Drosophila melanogaster, GI17865652, Length=118, Percent_Identity=41.5254237288136, Blast_Score=112, Evalue=7e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053 [H]

Pfam domain/function: PF01597 GCV_H [H]

EC number: NA

Molecular weight: Translated: 14479; Mature: 14348

Theoretical pI: Translated: 3.77; Mature: 3.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEINQNYLYTDSNLWVEVKGNIARVGIDDYSQDEFGEIVYVDLPKLEQEYAKDDEICVI
CCCCCCCEEEECCCEEEEECCCEEEEECCCCCCCCCCCEEEEECCCHHHHHCCCCCEEEE
ESVKTASDIYTPISGKVVKINQQLLDNPKLINESCYDNAWLFEIEIADLDELKSLINPQD
ECCCCHHHHCCCCCCEEEEECHHHCCCCCCCCCCCCCCEEEEEEEECCHHHHHHHCCCCH
YQSYVS
HHHHCC
>Mature Secondary Structure 
AEINQNYLYTDSNLWVEVKGNIARVGIDDYSQDEFGEIVYVDLPKLEQEYAKDDEICVI
CCCCCCEEEECCCEEEEECCCEEEEECCCCCCCCCCCEEEEECCCHHHHHCCCCCEEEE
ESVKTASDIYTPISGKVVKINQQLLDNPKLINESCYDNAWLFEIEIADLDELKSLINPQD
ECCCCHHHHCCCCCCEEEEECHHHCCCCCCCCCCCCCCEEEEEEEECCHHHHHHHCCCCH
YQSYVS
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA