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The map label for this gene is lipA [H]

Identifier: 89256278

GI number: 89256278

Start: 899831

End: 900814

Strand: Direct

Name: lipA [H]

Synonym: FTL_0927

Alternate gene names: 89256278

Gene position: 899831-900814 (Clockwise)

Preceding gene: 89256276

Following gene: 89256279

Centisome position: 47.46

GC content: 34.96

Gene sequence:

>984_bases
ATGAAAGAAATATCTGGTATAAAAGTTAAAGTTGAAAGTGGCTCTAAGTACACTACTGATCATGGTTTTTATGCTGTCAA
AGATGGTATTAGAAATAAGAAAGAAAATGCTGTGCATGTACGTAAACCAGATTGGTTGAAAGTGCAAAAACAAGATTCTA
AAGAATACCTAAAAGTTAAATCCATTACAAAAAAACATAAACTTTCAACAGTTTGTGAAGAAGCAAGGTGCCCTAATATT
AATGAGTGCTGGTCTCATGGTACAGCAACAATTATGTTAATGGGGAGTGTATGTACTAGAGCTTGTAAATTTTGTTCTGT
AGATACAGGTAATCCAAAAGGTTGGTTAGATAAAGATGAACCTATGAATGCTGCTGAAAGTGTAAAGCTCATGGGACTTG
AATATGTTGTATTGACATCTGTAGATCGTGATGATTTAGAAGATGGTGGTGCTGGACACTATGCAGCGACTATTACAGCT
ATCAAAAATCTTGATGAGAATATTAAAGTAGAAGCATTGACTCCAGATTTTGCTGGTATTAACGAAAATATTGATAAAAT
CATAAATACAAAAGTAGATGTAATTGCACAAAATATCGAGACTGTAGAGCGCTTGACTCATCCAGTACGTGATCCACGTG
CAGGGTACTGGCAAACATTAAATTTCTTAAAATATGTTAAACAAAAATCACCAAATGTGCTTACTAAAACTAGTATTATG
GTAGGTTTAGGTGAGACTGATGAAGAAATTTACAAAACTATGGATGATGCGCGAAGTGTTGGAGTTGATATAATAACGCT
TGGGCAATATATGCAACCAACAAAGCATCATTTGAGTGTTGAGAGGTTTGTGACACCACAACAATTCGAAGAGTATCGCA
AAGTGGGTCTCGAAAAAGGCTTCTTAGAAGTGGCATCAGGGCCTATGGTAAGATCAAGCTATAGAGCTGATAGAGTTTTC
AAAAGGAATAATTTAGATTTGTAA

Upstream 100 bases:

>100_bases
CTTATGAATATAGATAATCTTAAACTAATTATTTATAAATAGGCGTTTATCTTTTATAATGTAGAGAAATTATAGCTTAA
GTTAATATGCTAAAAATATT

Downstream 100 bases:

>100_bases
AATCAAAAAATATGTAAAGGTGAATAAAAATGGCAAAAGTAGCGGTAGTATTATCTGGATGTGGTTATCTCGATGGTTCA
GAAATACACGAAACTGTATT

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA [H]

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MKEISGIKVKVESGSKYTTDHGFYAVKDGIRNKKENAVHVRKPDWLKVQKQDSKEYLKVKSITKKHKLSTVCEEARCPNI
NECWSHGTATIMLMGSVCTRACKFCSVDTGNPKGWLDKDEPMNAAESVKLMGLEYVVLTSVDRDDLEDGGAGHYAATITA
IKNLDENIKVEALTPDFAGINENIDKIINTKVDVIAQNIETVERLTHPVRDPRAGYWQTLNFLKYVKQKSPNVLTKTSIM
VGLGETDEEIYKTMDDARSVGVDIITLGQYMQPTKHHLSVERFVTPQQFEEYRKVGLEKGFLEVASGPMVRSSYRADRVF
KRNNLDL

Sequences:

>Translated_327_residues
MKEISGIKVKVESGSKYTTDHGFYAVKDGIRNKKENAVHVRKPDWLKVQKQDSKEYLKVKSITKKHKLSTVCEEARCPNI
NECWSHGTATIMLMGSVCTRACKFCSVDTGNPKGWLDKDEPMNAAESVKLMGLEYVVLTSVDRDDLEDGGAGHYAATITA
IKNLDENIKVEALTPDFAGINENIDKIINTKVDVIAQNIETVERLTHPVRDPRAGYWQTLNFLKYVKQKSPNVLTKTSIM
VGLGETDEEIYKTMDDARSVGVDIITLGQYMQPTKHHLSVERFVTPQQFEEYRKVGLEKGFLEVASGPMVRSSYRADRVF
KRNNLDL
>Mature_327_residues
MKEISGIKVKVESGSKYTTDHGFYAVKDGIRNKKENAVHVRKPDWLKVQKQDSKEYLKVKSITKKHKLSTVCEEARCPNI
NECWSHGTATIMLMGSVCTRACKFCSVDTGNPKGWLDKDEPMNAAESVKLMGLEYVVLTSVDRDDLEDGGAGHYAATITA
IKNLDENIKVEALTPDFAGINENIDKIINTKVDVIAQNIETVERLTHPVRDPRAGYWQTLNFLKYVKQKSPNVLTKTSIM
VGLGETDEEIYKTMDDARSVGVDIITLGQYMQPTKHHLSVERFVTPQQFEEYRKVGLEKGFLEVASGPMVRSSYRADRVF
KRNNLDL

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives [H]

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family [H]

Homologues:

Organism=Homo sapiens, GI37577166, Length=296, Percent_Identity=51.6891891891892, Blast_Score=310, Evalue=1e-84,
Organism=Homo sapiens, GI37577164, Length=253, Percent_Identity=51.7786561264822, Blast_Score=263, Evalue=2e-70,
Organism=Escherichia coli, GI1786846, Length=285, Percent_Identity=47.3684210526316, Blast_Score=278, Evalue=3e-76,
Organism=Caenorhabditis elegans, GI32564533, Length=295, Percent_Identity=50.5084745762712, Blast_Score=291, Evalue=4e-79,
Organism=Saccharomyces cerevisiae, GI6324770, Length=306, Percent_Identity=49.6732026143791, Blast_Score=293, Evalue=2e-80,
Organism=Drosophila melanogaster, GI221513272, Length=299, Percent_Identity=54.5150501672241, Blast_Score=316, Evalue=1e-86,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.8.1.8 [H]

Molecular weight: Translated: 36867; Mature: 36867

Theoretical pI: Translated: 8.12; Mature: 8.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEISGIKVKVESGSKYTTDHGFYAVKDGIRNKKENAVHVRKPDWLKVQKQDSKEYLKVK
CCCCCCCEEEECCCCEEECCCCEEEEHHHCCCCCCCEEEECCCCCEEEECCCCHHHHHHH
SITKKHKLSTVCEEARCPNINECWSHGTATIMLMGSVCTRACKFCSVDTGNPKGWLDKDE
HHHHHHHHHHHHHHHCCCCHHHHHCCCCEEEEEHHHHHHHHHHHCCCCCCCCCCCCCCCC
PMNAAESVKLMGLEYVVLTSVDRDDLEDGGAGHYAATITAIKNLDENIKVEALTPDFAGI
CCCHHHHEEHCCEEEEEEECCCHHCCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCCC
NENIDKIINTKVDVIAQNIETVERLTHPVRDPRAGYWQTLNFLKYVKQKSPNVLTKTSIM
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEE
VGLGETDEEIYKTMDDARSVGVDIITLGQYMQPTKHHLSVERFVTPQQFEEYRKVGLEKG
EECCCCHHHHHHHHHHHHHCCCEEEECCHHCCCHHHHHHHHHCCCHHHHHHHHHHCHHCC
FLEVASGPMVRSSYRADRVFKRNNLDL
HHHHHCCCCHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKEISGIKVKVESGSKYTTDHGFYAVKDGIRNKKENAVHVRKPDWLKVQKQDSKEYLKVK
CCCCCCCEEEECCCCEEECCCCEEEEHHHCCCCCCCEEEECCCCCEEEECCCCHHHHHHH
SITKKHKLSTVCEEARCPNINECWSHGTATIMLMGSVCTRACKFCSVDTGNPKGWLDKDE
HHHHHHHHHHHHHHHCCCCHHHHHCCCCEEEEEHHHHHHHHHHHCCCCCCCCCCCCCCCC
PMNAAESVKLMGLEYVVLTSVDRDDLEDGGAGHYAATITAIKNLDENIKVEALTPDFAGI
CCCHHHHEEHCCEEEEEEECCCHHCCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCCC
NENIDKIINTKVDVIAQNIETVERLTHPVRDPRAGYWQTLNFLKYVKQKSPNVLTKTSIM
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEE
VGLGETDEEIYKTMDDARSVGVDIITLGQYMQPTKHHLSVERFVTPQQFEEYRKVGLEKG
EECCCCHHHHHHHHHHHHHCCCEEEECCHHCCCHHHHHHHHHCCCHHHHHHHHHHCHHCC
FLEVASGPMVRSSYRADRVFKRNNLDL
HHHHHCCCCHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA