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The map label for this gene is ansA [H]

Identifier: 89256215

GI number: 89256215

Start: 835833

End: 836870

Strand: Direct

Name: ansA [H]

Synonym: FTL_0855

Alternate gene names: 89256215

Gene position: 835833-836870 (Clockwise)

Preceding gene: 89256214

Following gene: 89256216

Centisome position: 44.08

GC content: 33.53

Gene sequence:

>1038_bases
ATGAAAGATCTTGAAAATCGTGCTAATAAAAAAATTTTAGTTCTATATACTGGTGGTACTATAGGTATGGTTAGTACTGA
ACAAGGCTATGATGTCAAACCTGGTTATCTTAGCGAGACAATTGCGGGTATTAGAGATTTTTATCATTATAATATGCCAC
AATTTGAGATTAAAGAGTACAGTGAACTTATAGATTCATCAAATATTAATCCTAGAAAATGGCTTTCATTAGCTTTAGAT
ATTATTAAAACTTATGAGGATTATGATGGCTTTGTAATTCTACATGGTACAGACACTCTTGCTTATACTGCTTCTGTATT
ATCATTTATGCTTGGTGAACTGCAAAAACCAGTAATTGTCACAGGATCACAAATTCCAATCTCAAAGATCAGATCAGATG
CAATATCAAATATTCTTAATAGTCTAATATTTGCTTGTAGTGATGATATCAAAGAAGTATGTGTATATTTCAATCAAAAG
CTAATGCGTGGTAATAGAACTACAAAAATATCAGCAACTGATTTTGATGCTTTTGCTTCACCAAACTATCCTGCATTGGC
AAGAGTTGGTATAGATATAGTCGTTAGGCAAGAAAAATTATGGCAGCGTGCTGATACCTTTAGTCAACCTAAGTTAGAAA
CTTTTGCTGTGCCAAAGATTGCTATTTTAAGTGTCTTTCCTGGCATGGGTAATGATATTTTAGAAGCTGTACTTGAGCCA
CCGTTACAAGGCTTAATTCTCAAGACTTATGGTGCTGGTAATATGATTAATGATCCAAGTATACATATGACACTAAAAAA
AGCTAATGATAGAGGTGTTGTGATAGTAAACTGTACTCAATGTTTGTATGGAAGTGTGAAAATGGATGCTTACAAGGCTG
CTAGAGGACTTGTTGAAGCAGGCGTGGTTTCTGGTTATGATATGACAGATGAAGCAGCTTTAGGAAAGTTATTTTATTTA
TTAAGCCAGCCAAATATGACACAACAAGATGTCAAAGAAGCTTTTAATGTTTCATTACAAGGCGAAGTAACGATATAA

Upstream 100 bases:

>100_bases
GTAACATGGGGCTGGGTAAAGGGTCACAGTGGTAATGCTGGCAATGAAAAGGCGGATGAGTTAGCAAACAAAGCTATTGC
TGAGCTAATAGGGAAATAAA

Downstream 100 bases:

>100_bases
CTTAAATATAAATAGTAAAAAGCTTATACGTATAGCTAGCTAATACTAATAATAATTTCAAGGAGATAAAATGAGTGATA
TTTCTGAGTTGATAAAAGGC

Product: L-asparaginase

Products: NA

Alternate protein names: L-asparaginase I; L-ASNase I; L-asparagine amidohydrolase I [H]

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MKDLENRANKKILVLYTGGTIGMVSTEQGYDVKPGYLSETIAGIRDFYHYNMPQFEIKEYSELIDSSNINPRKWLSLALD
IIKTYEDYDGFVILHGTDTLAYTASVLSFMLGELQKPVIVTGSQIPISKIRSDAISNILNSLIFACSDDIKEVCVYFNQK
LMRGNRTTKISATDFDAFASPNYPALARVGIDIVVRQEKLWQRADTFSQPKLETFAVPKIAILSVFPGMGNDILEAVLEP
PLQGLILKTYGAGNMINDPSIHMTLKKANDRGVVIVNCTQCLYGSVKMDAYKAARGLVEAGVVSGYDMTDEAALGKLFYL
LSQPNMTQQDVKEAFNVSLQGEVTI

Sequences:

>Translated_345_residues
MKDLENRANKKILVLYTGGTIGMVSTEQGYDVKPGYLSETIAGIRDFYHYNMPQFEIKEYSELIDSSNINPRKWLSLALD
IIKTYEDYDGFVILHGTDTLAYTASVLSFMLGELQKPVIVTGSQIPISKIRSDAISNILNSLIFACSDDIKEVCVYFNQK
LMRGNRTTKISATDFDAFASPNYPALARVGIDIVVRQEKLWQRADTFSQPKLETFAVPKIAILSVFPGMGNDILEAVLEP
PLQGLILKTYGAGNMINDPSIHMTLKKANDRGVVIVNCTQCLYGSVKMDAYKAARGLVEAGVVSGYDMTDEAALGKLFYL
LSQPNMTQQDVKEAFNVSLQGEVTI
>Mature_345_residues
MKDLENRANKKILVLYTGGTIGMVSTEQGYDVKPGYLSETIAGIRDFYHYNMPQFEIKEYSELIDSSNINPRKWLSLALD
IIKTYEDYDGFVILHGTDTLAYTASVLSFMLGELQKPVIVTGSQIPISKIRSDAISNILNSLIFACSDDIKEVCVYFNQK
LMRGNRTTKISATDFDAFASPNYPALARVGIDIVVRQEKLWQRADTFSQPKLETFAVPKIAILSVFPGMGNDILEAVLEP
PLQGLILKTYGAGNMINDPSIHMTLKKANDRGVVIVNCTQCLYGSVKMDAYKAARGLVEAGVVSGYDMTDEAALGKLFYL
LSQPNMTQQDVKEAFNVSLQGEVTI

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Homo sapiens, GI122937331, Length=358, Percent_Identity=40.7821229050279, Blast_Score=251, Evalue=7e-67,
Organism=Escherichia coli, GI1788065, Length=341, Percent_Identity=47.2140762463343, Blast_Score=308, Evalue=3e-85,
Organism=Escherichia coli, GI1789327, Length=248, Percent_Identity=28.2258064516129, Blast_Score=84, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI193207372, Length=360, Percent_Identity=43.0555555555556, Blast_Score=278, Evalue=2e-75,
Organism=Caenorhabditis elegans, GI17558278, Length=360, Percent_Identity=43.0555555555556, Blast_Score=278, Evalue=2e-75,
Organism=Caenorhabditis elegans, GI17558280, Length=360, Percent_Identity=43.0555555555556, Blast_Score=278, Evalue=3e-75,
Organism=Caenorhabditis elegans, GI193207374, Length=360, Percent_Identity=43.0555555555556, Blast_Score=278, Evalue=3e-75,
Organism=Drosophila melanogaster, GI24639609, Length=366, Percent_Identity=40.1639344262295, Blast_Score=259, Evalue=1e-69,
Organism=Drosophila melanogaster, GI24645530, Length=357, Percent_Identity=38.375350140056, Blast_Score=247, Evalue=6e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006033
- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 38205; Mature: 38205

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS00144 ASN_GLN_ASE_1 ; PS00917 ASN_GLN_ASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDLENRANKKILVLYTGGTIGMVSTEQGYDVKPGYLSETIAGIRDFYHYNMPQFEIKEY
CCCCHHHCCCEEEEEEECCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHH
SELIDSSNINPRKWLSLALDIIKTYEDYDGFVILHGTDTLAYTASVLSFMLGELQKPVIV
HHHHHCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCEEE
TGSQIPISKIRSDAISNILNSLIFACSDDIKEVCVYFNQKLMRGNRTTKISATDFDAFAS
ECCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCEEEECCCCHHCCC
PNYPALARVGIDIVVRQEKLWQRADTFSQPKLETFAVPKIAILSVFPGMGNDILEAVLEP
CCCCEEHHCCEEEEEEHHHHHHHHCCCCCCCCHHHCCCCEEEEECCCCCCHHHHHHHHCC
PLQGLILKTYGAGNMINDPSIHMTLKKANDRGVVIVNCTQCLYGSVKMDAYKAARGLVEA
CCCCEEEEEECCCCCCCCCCEEEEEEECCCCCEEEEEEHHHHCCCCHHHHHHHHHHHHHH
GVVSGYDMTDEAALGKLFYLLSQPNMTQQDVKEAFNVSLQGEVTI
HCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHCCCEEEEEEC
>Mature Secondary Structure
MKDLENRANKKILVLYTGGTIGMVSTEQGYDVKPGYLSETIAGIRDFYHYNMPQFEIKEY
CCCCHHHCCCEEEEEEECCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHH
SELIDSSNINPRKWLSLALDIIKTYEDYDGFVILHGTDTLAYTASVLSFMLGELQKPVIV
HHHHHCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCEEE
TGSQIPISKIRSDAISNILNSLIFACSDDIKEVCVYFNQKLMRGNRTTKISATDFDAFAS
ECCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCEEEECCCCHHCCC
PNYPALARVGIDIVVRQEKLWQRADTFSQPKLETFAVPKIAILSVFPGMGNDILEAVLEP
CCCCEEHHCCEEEEEEHHHHHHHHCCCCCCCCHHHCCCCEEEEECCCCCCHHHHHHHHCC
PLQGLILKTYGAGNMINDPSIHMTLKKANDRGVVIVNCTQCLYGSVKMDAYKAARGLVEA
CCCCEEEEEECCCCCCCCCCEEEEEEECCCCCEEEEEEHHHHCCCCHHHHHHHHHHHHHH
GVVSGYDMTDEAALGKLFYLLSQPNMTQQDVKEAFNVSLQGEVTI
HCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]