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The map label for this gene is rsmB [H]

Identifier: 89054474

GI number: 89054474

Start: 1979289

End: 1980452

Strand: Direct

Name: rsmB [H]

Synonym: Jann_1983

Alternate gene names: 89054474

Gene position: 1979289-1980452 (Clockwise)

Preceding gene: 89054473

Following gene: 89054475

Centisome position: 45.84

GC content: 64.78

Gene sequence:

>1164_bases
ATGCAACCTGCCGCGCGCTATGCCGCCGCAATCGAGGTATTGGACGCTTGGTGCGATGGCTTGGCCGTCGAACAGGCGCT
GACGCGATGGGCACGCGGCGCGCGGTATGCCGGGTCAAAGGACCGGGCTGCGGTGCGCGATCACGTCTATGATGTTTTGC
GCCAGAAGGGATCATGCGAGGCCCTCGGGAACGGGACGGGGCGTGGATTAATTCTGGGCCTCCTGCGGATCCAAGGGCGT
GATGCCGAGGCGGTTTTTTCTGGTGTCGGTCACGCGCCGGACCCGCTGAGTGAACGGGAAAAGTCCTCGCCGCAGACCTC
GCTGGACCCCCCCATGAACATCCCCGATTGGACGCTGCCTCATCTGAGATCCCGCGTGCCAGATGCTCTGCCGGTGCTGT
TGTCGAGCCTCACGCACCGCGCGCCGCTCTGGCTTCGGGTGAACCTGTCACGGACCACACGCGCGGCGGTTGCGGACGCG
CTGGCTGACGATGGCATTGAAACGCGCGCCCATGAGGCCGTCCCAACCGCGCTTGAGGTGACGGACGGTCCGCGCAAATT
GCGTCAGTCCGCCGCCTACCTCTCGGGCCTGGTGGAGCCTCAGGACCTGTCCGTGCAATGGGCTATGCAGCGCGTGGACT
GGCCCGCCGACGGGCGCATTCTGGACTATTGCGCGGGTGGCGGCGGCAAGGCGTTGGCCATTGCGGACCGGACTGGTGCG
GACCTGTTTGCCCATGATGCGTTGCCGCAGAGGATGGCCGACCTTGGTCCGCGCGCGGGGCGGGCTGGGGCGAAGATCAC
CACGTTGGAAAGCGACGACTTTGGGCAAGAGGATCCGTTTGATCTGGTATTGACGGATGTGCCCTGTTCGGGCTCGGGCA
CGTGGCGGCGTGACCCGGAGGCCAAATGGCGGCTGACCTCGCAAGGCCTTGAAGATCTTGTCAAAACCCAAAGTGCGATC
CTGGACGCGGCGGCTGATCTCGTGCTGCCGGGGGGACGGTTGGTTTACATGACCTGCTCTCTGTTCGAGGTGGAAAATGA
GGCGCAGATCACGGCCTTTCTGGCACGTCACCCCGATTGGCAAGCAGGTGCCGCGCATGCCGATACCCCGCTTACAGCCT
CCGACGGCTTCTATTCGGCGGAGCTGCACCGCATCATCTCCTAG

Upstream 100 bases:

>100_bases
GAAAGAAAGCCATGTGCATGATGTGCAGATCACTCGCGAAAGCCCAAATTATCGTGCGGGATGAATATCTTGTACGCCGA
CATTAAAGTGATACCTTAGG

Downstream 100 bases:

>100_bases
CCAAGTGGTGAGAAATCATACAGTCTTAAGGTTAATTTAACCATTTTGGCAGAACGCTCGGCAGGCAGGCAGATCCACGG
CGCGAGCGATCTATCTGACC

Product: Fmu (Sun)

Products: NA

Alternate protein names: 16S rRNA m5C967 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmB [H]

Number of amino acids: Translated: 387; Mature: 387

Protein sequence:

>387_residues
MQPAARYAAAIEVLDAWCDGLAVEQALTRWARGARYAGSKDRAAVRDHVYDVLRQKGSCEALGNGTGRGLILGLLRIQGR
DAEAVFSGVGHAPDPLSEREKSSPQTSLDPPMNIPDWTLPHLRSRVPDALPVLLSSLTHRAPLWLRVNLSRTTRAAVADA
LADDGIETRAHEAVPTALEVTDGPRKLRQSAAYLSGLVEPQDLSVQWAMQRVDWPADGRILDYCAGGGGKALAIADRTGA
DLFAHDALPQRMADLGPRAGRAGAKITTLESDDFGQEDPFDLVLTDVPCSGSGTWRRDPEAKWRLTSQGLEDLVKTQSAI
LDAAADLVLPGGRLVYMTCSLFEVENEAQITAFLARHPDWQAGAAHADTPLTASDGFYSAELHRIIS

Sequences:

>Translated_387_residues
MQPAARYAAAIEVLDAWCDGLAVEQALTRWARGARYAGSKDRAAVRDHVYDVLRQKGSCEALGNGTGRGLILGLLRIQGR
DAEAVFSGVGHAPDPLSEREKSSPQTSLDPPMNIPDWTLPHLRSRVPDALPVLLSSLTHRAPLWLRVNLSRTTRAAVADA
LADDGIETRAHEAVPTALEVTDGPRKLRQSAAYLSGLVEPQDLSVQWAMQRVDWPADGRILDYCAGGGGKALAIADRTGA
DLFAHDALPQRMADLGPRAGRAGAKITTLESDDFGQEDPFDLVLTDVPCSGSGTWRRDPEAKWRLTSQGLEDLVKTQSAI
LDAAADLVLPGGRLVYMTCSLFEVENEAQITAFLARHPDWQAGAAHADTPLTASDGFYSAELHRIIS
>Mature_387_residues
MQPAARYAAAIEVLDAWCDGLAVEQALTRWARGARYAGSKDRAAVRDHVYDVLRQKGSCEALGNGTGRGLILGLLRIQGR
DAEAVFSGVGHAPDPLSEREKSSPQTSLDPPMNIPDWTLPHLRSRVPDALPVLLSSLTHRAPLWLRVNLSRTTRAAVADA
LADDGIETRAHEAVPTALEVTDGPRKLRQSAAYLSGLVEPQDLSVQWAMQRVDWPADGRILDYCAGGGGKALAIADRTGA
DLFAHDALPQRMADLGPRAGRAGAKITTLESDDFGQEDPFDLVLTDVPCSGSGTWRRDPEAKWRLTSQGLEDLVKTQSAI
LDAAADLVLPGGRLVYMTCSLFEVENEAQITAFLARHPDWQAGAAHADTPLTASDGFYSAELHRIIS

Specific function: Specifically methylates the cytosine at position 967 (m5C967) of 16S rRNA [H]

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI76150625, Length=218, Percent_Identity=30.7339449541284, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI76150623, Length=218, Percent_Identity=30.7339449541284, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI2367212, Length=334, Percent_Identity=30.5389221556886, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI87081985, Length=274, Percent_Identity=29.1970802919708, Blast_Score=82, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI17536757, Length=228, Percent_Identity=31.5789473684211, Blast_Score=73, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6324268, Length=233, Percent_Identity=29.6137339055794, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI22024126, Length=218, Percent_Identity=29.3577981651376, Blast_Score=65, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR006027
- InterPro:   IPR004573 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu; PF01029 NusB [H]

EC number: =2.1.1.176 [H]

Molecular weight: Translated: 41708; Mature: 41708

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQPAARYAAAIEVLDAWCDGLAVEQALTRWARGARYAGSKDRAAVRDHVYDVLRQKGSCE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCC
ALGNGTGRGLILGLLRIQGRDAEAVFSGVGHAPDPLSEREKSSPQTSLDPPMNIPDWTLP
CCCCCCCCHHHEEEHHHCCCCHHHHHHCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCHH
HLRSRVPDALPVLLSSLTHRAPLWLRVNLSRTTRAAVADALADDGIETRAHEAVPTALEV
HHHHHCCHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHCCCCHHHHHHHCCCEEEE
TDGPRKLRQSAAYLSGLVEPQDLSVQWAMQRVDWPADGRILDYCAGGGGKALAIADRTGA
CCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCEEEEECCCCCCEEEEECCCCC
DLFAHDALPQRMADLGPRAGRAGAKITTLESDDFGQEDPFDLVLTDVPCSGSGTWRRDPE
CHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCC
AKWRLTSQGLEDLVKTQSAILDAAADLVLPGGRLVYMTCSLFEVENEAQITAFLARHPDW
CCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEECCCCHHEEHHHHCCCCC
QAGAAHADTPLTASDGFYSAELHRIIS
CCCCCCCCCCCCCCCCCHHHHHHHHCC
>Mature Secondary Structure
MQPAARYAAAIEVLDAWCDGLAVEQALTRWARGARYAGSKDRAAVRDHVYDVLRQKGSCE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCC
ALGNGTGRGLILGLLRIQGRDAEAVFSGVGHAPDPLSEREKSSPQTSLDPPMNIPDWTLP
CCCCCCCCHHHEEEHHHCCCCHHHHHHCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCHH
HLRSRVPDALPVLLSSLTHRAPLWLRVNLSRTTRAAVADALADDGIETRAHEAVPTALEV
HHHHHCCHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHCCCCHHHHHHHCCCEEEE
TDGPRKLRQSAAYLSGLVEPQDLSVQWAMQRVDWPADGRILDYCAGGGGKALAIADRTGA
CCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCEEEEECCCCCCEEEEECCCCC
DLFAHDALPQRMADLGPRAGRAGAKITTLESDDFGQEDPFDLVLTDVPCSGSGTWRRDPE
CHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCC
AKWRLTSQGLEDLVKTQSAILDAAADLVLPGGRLVYMTCSLFEVENEAQITAFLARHPDW
CCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEECCCCHHEEHHHHCCCCC
QAGAAHADTPLTASDGFYSAELHRIIS
CCCCCCCCCCCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA