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The map label for this gene is rlmE

Identifier: 89054472

GI number: 89054472

Start: 1977006

End: 1977719

Strand: Direct

Name: rlmE

Synonym: Jann_1981

Alternate gene names: 89054472

Gene position: 1977006-1977719 (Clockwise)

Preceding gene: 89054471

Following gene: 89054473

Centisome position: 45.79

GC content: 64.01

Gene sequence:

>714_bases
ATGGTCAAGAAGACAAGCGGGCGCGGGCAGCGCGATTTGAAGGTCAAGGTCAAGACCGCGCGCGGGCGCAAGCTGTCGTC
GACGCGCTGGTTAGAGCGGCAGTTGAACGATCCCTATGTGGCCGCCGCAAGGCGGGACGGCTATCGCGGGCGGGCGGCCT
ATAAGATCATCGACCTGGATGACAAATACCGGTTTCTCGTTCCGGGTGCCCGGGTGGTGGATCTGGGCTGCGCGCCGGGC
GGCTGGTGTCAGGTCGCCGTGAAGCGGGTGAATGCGCTGGGTGAGAAGAGTGGCAAGGCCGTCGGGCGGATCATCGGGCT
GGATTTGCAGGAAATGGAGCCGATTGCGGGCTGTGAGCTGCATCAGCTCGACTTCATGGAAGACGATGCGGACCTTAAGG
TGAAGGACTGGCTTGGCGGTCGCGCCGATGTGGTGATGAGCGATATGGCGGCCTCGGCCTCGGGTCACAAGCAGACCGAT
CATATGCGCATCATGGCGTTGTGTGAGGCCGCCGCAGAGCTTGCCTTCGATGTGTTGGAGCCGGGCGGGACCTTCGTGGC
CAAGGTTTTGGCGGGCGGCGCCGAGGGCACGCTGCAGCGCCTTCTGAAGCAGCGCTTCAAGAAGGTGGCGAATGTGAAGC
CCGGGGCATCGCGCGCCGACAGTTCCGAGAAATTCGTGGTTGCGACGGGCTTCCGCGGTCGGGACGAGGCCTGA

Upstream 100 bases:

>100_bases
GATCTTGGGCAAAGGCCTCATCTGCTGCGCGCGGGGGCAGGTGTTCGGATTGCTTTTTTGGCAAGGTGAAAGAGGCGCGT
TGGCGTGCTTTGGGTGAGTG

Downstream 100 bases:

>100_bases
GGCACACCAGATTTAGGGCTTGCCCCGCTGGCGTCCCCATGGGATAGCGACGTGCCAACCGATCCCCCTCCAAGGAGCAC
CCAGATGGAGATTCGCGAGG

Product: ribosomal RNA methyltransferase RrmJ/FtsJ

Products: NA

Alternate protein names: 23S rRNA Um2552 methyltransferase; rRNA (uridine-2'-O-)-methyltransferase

Number of amino acids: Translated: 237; Mature: 237

Protein sequence:

>237_residues
MVKKTSGRGQRDLKVKVKTARGRKLSSTRWLERQLNDPYVAAARRDGYRGRAAYKIIDLDDKYRFLVPGARVVDLGCAPG
GWCQVAVKRVNALGEKSGKAVGRIIGLDLQEMEPIAGCELHQLDFMEDDADLKVKDWLGGRADVVMSDMAASASGHKQTD
HMRIMALCEAAAELAFDVLEPGGTFVAKVLAGGAEGTLQRLLKQRFKKVANVKPGASRADSSEKFVVATGFRGRDEA

Sequences:

>Translated_237_residues
MVKKTSGRGQRDLKVKVKTARGRKLSSTRWLERQLNDPYVAAARRDGYRGRAAYKIIDLDDKYRFLVPGARVVDLGCAPG
GWCQVAVKRVNALGEKSGKAVGRIIGLDLQEMEPIAGCELHQLDFMEDDADLKVKDWLGGRADVVMSDMAASASGHKQTD
HMRIMALCEAAAELAFDVLEPGGTFVAKVLAGGAEGTLQRLLKQRFKKVANVKPGASRADSSEKFVVATGFRGRDEA
>Mature_237_residues
MVKKTSGRGQRDLKVKVKTARGRKLSSTRWLERQLNDPYVAAARRDGYRGRAAYKIIDLDDKYRFLVPGARVVDLGCAPG
GWCQVAVKRVNALGEKSGKAVGRIIGLDLQEMEPIAGCELHQLDFMEDDADLKVKDWLGGRADVVMSDMAASASGHKQTD
HMRIMALCEAAAELAFDVLEPGGTFVAKVLAGGAEGTLQRLLKQRFKKVANVKPGASRADSSEKFVVATGFRGRDEA

Specific function: Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit

COG id: COG0293

COG function: function code J; 23S rRNA methylase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmE family

Homologues:

Organism=Homo sapiens, GI7019377, Length=207, Percent_Identity=42.0289855072464, Blast_Score=177, Evalue=6e-45,
Organism=Homo sapiens, GI29029591, Length=196, Percent_Identity=33.6734693877551, Blast_Score=104, Evalue=6e-23,
Organism=Homo sapiens, GI29029589, Length=196, Percent_Identity=33.6734693877551, Blast_Score=104, Evalue=6e-23,
Organism=Homo sapiens, GI7110661, Length=196, Percent_Identity=33.6734693877551, Blast_Score=104, Evalue=6e-23,
Organism=Homo sapiens, GI194097365, Length=208, Percent_Identity=33.1730769230769, Blast_Score=100, Evalue=1e-21,
Organism=Escherichia coli, GI1789569, Length=213, Percent_Identity=41.3145539906103, Blast_Score=147, Evalue=6e-37,
Organism=Caenorhabditis elegans, GI17553474, Length=211, Percent_Identity=36.0189573459716, Blast_Score=141, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17554650, Length=200, Percent_Identity=35, Blast_Score=108, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI71987561, Length=206, Percent_Identity=34.9514563106796, Blast_Score=108, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17553860, Length=206, Percent_Identity=34.9514563106796, Blast_Score=108, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI71987550, Length=206, Percent_Identity=34.9514563106796, Blast_Score=108, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6319535, Length=199, Percent_Identity=35.1758793969849, Blast_Score=109, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6319796, Length=201, Percent_Identity=35.8208955223881, Blast_Score=98, Evalue=9e-22,
Organism=Saccharomyces cerevisiae, GI6321302, Length=103, Percent_Identity=38.8349514563107, Blast_Score=77, Evalue=2e-15,
Organism=Drosophila melanogaster, GI21356387, Length=222, Percent_Identity=38.7387387387387, Blast_Score=154, Evalue=7e-38,
Organism=Drosophila melanogaster, GI24647580, Length=198, Percent_Identity=36.3636363636364, Blast_Score=109, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24648639, Length=196, Percent_Identity=34.1836734693878, Blast_Score=108, Evalue=3e-24,
Organism=Drosophila melanogaster, GI18859957, Length=205, Percent_Identity=37.0731707317073, Blast_Score=106, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLME_JANSC (Q28QW4)

Other databases:

- EMBL:   CP000264
- RefSeq:   YP_509923.1
- ProteinModelPortal:   Q28QW4
- SMR:   Q28QW4
- STRING:   Q28QW4
- GeneID:   3934433
- GenomeReviews:   CP000264_GR
- KEGG:   jan:Jann_1981
- eggNOG:   COG0293
- HOGENOM:   HBG398270
- OMA:   SDMAANT
- PhylomeDB:   Q28QW4
- ProtClustDB:   CLSK933653
- BioCyc:   JSP290400:JANN_1981-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01547
- InterPro:   IPR015507
- InterPro:   IPR002877
- PANTHER:   PTHR10920
- PIRSF:   PIRSF005461

Pfam domain/function: PF01728 FtsJ

EC number: =2.1.1.166

Molecular weight: Translated: 25806; Mature: 25806

Theoretical pI: Translated: 10.07; Mature: 10.07

Prosite motif: NA

Important sites: ACT_SITE 188-188 BINDING 80-80 BINDING 82-82 BINDING 108-108 BINDING 124-124 BINDING 148-148

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKKTSGRGQRDLKVKVKTARGRKLSSTRWLERQLNDPYVAAARRDGYRGRAAYKIIDLD
CCCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEECC
DKYRFLVPGARVVDLGCAPGGWCQVAVKRVNALGEKSGKAVGRIIGLDLQEMEPIAGCEL
CCEEEEECCCEEEEECCCCCHHHHHHHHHHHHHHHCCCHHHHHHCCCCHHHCCCCCCCCE
HQLDFMEDDADLKVKDWLGGRADVVMSDMAASASGHKQTDHMRIMALCEAAAELAFDVLE
EECCCCCCCCCEEEHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHC
PGGTFVAKVLAGGAEGTLQRLLKQRFKKVANVKPGASRADSSEKFVVATGFRGRDEA
CCCHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCC
>Mature Secondary Structure
MVKKTSGRGQRDLKVKVKTARGRKLSSTRWLERQLNDPYVAAARRDGYRGRAAYKIIDLD
CCCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEECC
DKYRFLVPGARVVDLGCAPGGWCQVAVKRVNALGEKSGKAVGRIIGLDLQEMEPIAGCEL
CCEEEEECCCEEEEECCCCCHHHHHHHHHHHHHHHCCCHHHHHHCCCCHHHCCCCCCCCE
HQLDFMEDDADLKVKDWLGGRADVVMSDMAASASGHKQTDHMRIMALCEAAAELAFDVLE
EECCCCCCCCCEEEHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHC
PGGTFVAKVLAGGAEGTLQRLLKQRFKKVANVKPGASRADSSEKFVVATGFRGRDEA
CCCHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA