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The map label for this gene is pdhC [H]

Identifier: 89054179

GI number: 89054179

Start: 1670782

End: 1672107

Strand: Reverse

Name: pdhC [H]

Synonym: Jann_1688

Alternate gene names: 89054179

Gene position: 1672107-1670782 (Counterclockwise)

Preceding gene: 89054180

Following gene: 89054178

Centisome position: 38.72

GC content: 64.78

Gene sequence:

>1326_bases
ATGCCCATCGAACTTCTCATGCCCGCCCTCTCCCCCACGATGGAGGAAGGCACCCTGGCGAAATGGCTTGTCAAGGAAGG
CGACACCGTCAATTCCGGCGATCTGCTGGCCGAGATCGAGACCGACAAGGCGACGATGGAATTCGAGGCCGTCGATGAGG
GGATCATCGGCAAGATCCTGGTCCCGGAGGGGACCGAGAACGTGAAGGTCAACACCGCCATCGCCCTGATCGGCGAGGAA
GGCGACGACTTCAGCGCCGCGCCAGCGGCCCCGGCCGAAGATGCCGCCGCCGAGGAGGCCTCGCCAGAGGCCGAGGAGGT
GACCCCTTCAGAAGCGGCTCCTGCCGCGGCCTCTTCTGCGCCCGCTGCACCTGTCACCAAGGACGGAGGTCGCATCTTCG
CCTCCCCCCTCGCCCGCCGGATCGCCAAGGACAAAGGTCTGGATCTGAGCCAGATCAAGGGCTCCGGCCCCCATGGCCGC
ATCGTGAAGGTCGACGTGGAAGGTGCCAGCGCCGCGCCCAAGTCCGAAGCCCCCACCGCCAAATCCGAGGCCCCGAAAGC
CGCCGCACCCGCCGGTGGCGGCGCGATGCCCACCGGTCCATCCGCCGAGCAGGTCCTTAAGATGTATGAAGGCCGCGAGT
TTGAAGAGGTCAAACTCAACGGCATGCGCAAGACCGTCGCCGCCCGCCTGACCGAGGCCAAGCAGACGATCCCGCATTTC
TACCTGCGCCGCGACATTCAGCTGGACGCTCTGCTGAAATTCCGCTCCCAGCTCAACAAACAGCTGGAAGGCCGCGGCGT
GAAGCTTTCCGTCAACGACTTCGTCATCAAGGCCTGCGCCCTTGCGTTGCAGGCGGTGCCCGACGCCAACGCCGTCTGGG
CCGGTGACCGGATGATCAAACTGAAGCCATCCGATGTGGCCGTGGCCGTGGCCGTCGATGGCGGCCTCTTCACGCCGGTC
CTGAAGGATAGCGACAGCAAAAGCCTCTCGGCCCTCTCGGCTGAAATGAAGGACCTCGCCACCCGCGCCCGGGACGGCAA
GCTGGCCCCCCACGAATACGTGGGTGGCTCCTTTGCCATCTCCAATCTCGGCATGATGGGGATCGAGAATTTCGATGCCG
TCATCAACCCGCCCCATGGCGCGATTCTGGCTGTCGGTGCGGGCGTCAAGAAGCCTGTCGTCGGCGCGGATGGAGAGCTG
GCAGTCGCGACCGTTATGTCCACCACCCTGTCGGTCGATCACCGTGTCATTGACGGCGCCCTGGGCGCAGAGCTTTTGGC
GGCCATCAAGGACAATCTGGAAAACCCGATGGTCATGCTGGCCTGA

Upstream 100 bases:

>100_bases
CGACGCCATCAAGGCCTTGAAAAAAGGCGCCACGCCCCGCGCGCCCTTCACCCATATCACGCTCGAATAAGCGATACCCT
CTTCAAGGGAGACGATCCAA

Downstream 100 bases:

>100_bases
GGTCCTTTGAGCGCCCGTGTCGTGCGGGCGCCTCAGGCACTCCCATACCGCTCACCGCGCCGGTCTGCGCAGGGGTCGAC
CCCGACAATCCACGCTGCAC

Product: branched-chain alpha-keto acid dehydrogenase subunit E2

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 441; Mature: 440

Protein sequence:

>441_residues
MPIELLMPALSPTMEEGTLAKWLVKEGDTVNSGDLLAEIETDKATMEFEAVDEGIIGKILVPEGTENVKVNTAIALIGEE
GDDFSAAPAAPAEDAAAEEASPEAEEVTPSEAAPAAASSAPAAPVTKDGGRIFASPLARRIAKDKGLDLSQIKGSGPHGR
IVKVDVEGASAAPKSEAPTAKSEAPKAAAPAGGGAMPTGPSAEQVLKMYEGREFEEVKLNGMRKTVAARLTEAKQTIPHF
YLRRDIQLDALLKFRSQLNKQLEGRGVKLSVNDFVIKACALALQAVPDANAVWAGDRMIKLKPSDVAVAVAVDGGLFTPV
LKDSDSKSLSALSAEMKDLATRARDGKLAPHEYVGGSFAISNLGMMGIENFDAVINPPHGAILAVGAGVKKPVVGADGEL
AVATVMSTTLSVDHRVIDGALGAELLAAIKDNLENPMVMLA

Sequences:

>Translated_441_residues
MPIELLMPALSPTMEEGTLAKWLVKEGDTVNSGDLLAEIETDKATMEFEAVDEGIIGKILVPEGTENVKVNTAIALIGEE
GDDFSAAPAAPAEDAAAEEASPEAEEVTPSEAAPAAASSAPAAPVTKDGGRIFASPLARRIAKDKGLDLSQIKGSGPHGR
IVKVDVEGASAAPKSEAPTAKSEAPKAAAPAGGGAMPTGPSAEQVLKMYEGREFEEVKLNGMRKTVAARLTEAKQTIPHF
YLRRDIQLDALLKFRSQLNKQLEGRGVKLSVNDFVIKACALALQAVPDANAVWAGDRMIKLKPSDVAVAVAVDGGLFTPV
LKDSDSKSLSALSAEMKDLATRARDGKLAPHEYVGGSFAISNLGMMGIENFDAVINPPHGAILAVGAGVKKPVVGADGEL
AVATVMSTTLSVDHRVIDGALGAELLAAIKDNLENPMVMLA
>Mature_440_residues
PIELLMPALSPTMEEGTLAKWLVKEGDTVNSGDLLAEIETDKATMEFEAVDEGIIGKILVPEGTENVKVNTAIALIGEEG
DDFSAAPAAPAEDAAAEEASPEAEEVTPSEAAPAAASSAPAAPVTKDGGRIFASPLARRIAKDKGLDLSQIKGSGPHGRI
VKVDVEGASAAPKSEAPTAKSEAPKAAAPAGGGAMPTGPSAEQVLKMYEGREFEEVKLNGMRKTVAARLTEAKQTIPHFY
LRRDIQLDALLKFRSQLNKQLEGRGVKLSVNDFVIKACALALQAVPDANAVWAGDRMIKLKPSDVAVAVAVDGGLFTPVL
KDSDSKSLSALSAEMKDLATRARDGKLAPHEYVGGSFAISNLGMMGIENFDAVINPPHGAILAVGAGVKKPVVGADGELA
VATVMSTTLSVDHRVIDGALGAELLAAIKDNLENPMVMLA

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI31711992, Length=453, Percent_Identity=43.9293598233996, Blast_Score=345, Evalue=7e-95,
Organism=Homo sapiens, GI203098753, Length=464, Percent_Identity=39.2241379310345, Blast_Score=286, Evalue=2e-77,
Organism=Homo sapiens, GI203098816, Length=464, Percent_Identity=39.2241379310345, Blast_Score=286, Evalue=3e-77,
Organism=Homo sapiens, GI110671329, Length=435, Percent_Identity=29.4252873563218, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI260898739, Length=158, Percent_Identity=44.9367088607595, Blast_Score=135, Evalue=6e-32,
Organism=Homo sapiens, GI19923748, Length=226, Percent_Identity=32.3008849557522, Blast_Score=112, Evalue=1e-24,
Organism=Escherichia coli, GI1786946, Length=441, Percent_Identity=31.2925170068027, Blast_Score=185, Evalue=4e-48,
Organism=Escherichia coli, GI1786305, Length=425, Percent_Identity=33.1764705882353, Blast_Score=160, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17560088, Length=456, Percent_Identity=42.9824561403509, Blast_Score=320, Evalue=1e-87,
Organism=Caenorhabditis elegans, GI17538894, Length=315, Percent_Identity=35.8730158730159, Blast_Score=181, Evalue=7e-46,
Organism=Caenorhabditis elegans, GI17537937, Length=431, Percent_Identity=29.4663573085847, Blast_Score=172, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI25146366, Length=436, Percent_Identity=31.4220183486239, Blast_Score=171, Evalue=8e-43,
Organism=Saccharomyces cerevisiae, GI6324258, Length=450, Percent_Identity=40.4444444444444, Blast_Score=292, Evalue=9e-80,
Organism=Saccharomyces cerevisiae, GI6320352, Length=437, Percent_Identity=28.604118993135, Blast_Score=147, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6321632, Length=172, Percent_Identity=32.5581395348837, Blast_Score=80, Evalue=5e-16,
Organism=Drosophila melanogaster, GI20129315, Length=457, Percent_Identity=40.0437636761488, Blast_Score=283, Evalue=2e-76,
Organism=Drosophila melanogaster, GI24582497, Length=446, Percent_Identity=39.4618834080717, Blast_Score=268, Evalue=4e-72,
Organism=Drosophila melanogaster, GI18859875, Length=440, Percent_Identity=30.9090909090909, Blast_Score=171, Evalue=7e-43,
Organism=Drosophila melanogaster, GI24645909, Length=243, Percent_Identity=31.6872427983539, Blast_Score=120, Evalue=1e-27,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006257
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 45750; Mature: 45618

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPIELLMPALSPTMEEGTLAKWLVKEGDTVNSGDLLAEIETDKATMEFEAVDEGIIGKIL
CCHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCCEEEEEECCCCEEEEHHCCCCCEEEEE
VPEGTENVKVNTAIALIGEEGDDFSAAPAAPAEDAAAEEASPEAEEVTPSEAAPAAASSA
CCCCCCCEEEEEEEEEEECCCCCCCCCCCCCCCHHHHHCCCCCHHHCCCCCCCCCCCCCC
PAAPVTKDGGRIFASPLARRIAKDKGLDLSQIKGSGPHGRIVKVDVEGASAAPKSEAPTA
CCCCCCCCCCEEHHHHHHHHHHHHCCCCHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCC
KSEAPKAAAPAGGGAMPTGPSAEQVLKMYEGREFEEVKLNGMRKTVAARLTEAKQTIPHF
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
YLRRDIQLDALLKFRSQLNKQLEGRGVKLSVNDFVIKACALALQAVPDANAVWAGDRMIK
HHHCCCCHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHCCCCCCEEECCCEEEE
LKPSDVAVAVAVDGGLFTPVLKDSDSKSLSALSAEMKDLATRARDGKLAPHEYVGGSFAI
ECCCCEEEEEEECCCEECCEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCEEEH
SNLGMMGIENFDAVINPPHGAILAVGAGVKKPVVGADGELAVATVMSTTLSVDHRVIDGA
HHCCCCCCCCCCCEECCCCCEEEEECCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHH
LGAELLAAIKDNLENPMVMLA
HHHHHHHHHHHCCCCCEEEEC
>Mature Secondary Structure 
PIELLMPALSPTMEEGTLAKWLVKEGDTVNSGDLLAEIETDKATMEFEAVDEGIIGKIL
CHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCCEEEEEECCCCEEEEHHCCCCCEEEEE
VPEGTENVKVNTAIALIGEEGDDFSAAPAAPAEDAAAEEASPEAEEVTPSEAAPAAASSA
CCCCCCCEEEEEEEEEEECCCCCCCCCCCCCCCHHHHHCCCCCHHHCCCCCCCCCCCCCC
PAAPVTKDGGRIFASPLARRIAKDKGLDLSQIKGSGPHGRIVKVDVEGASAAPKSEAPTA
CCCCCCCCCCEEHHHHHHHHHHHHCCCCHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCC
KSEAPKAAAPAGGGAMPTGPSAEQVLKMYEGREFEEVKLNGMRKTVAARLTEAKQTIPHF
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
YLRRDIQLDALLKFRSQLNKQLEGRGVKLSVNDFVIKACALALQAVPDANAVWAGDRMIK
HHHCCCCHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHCCCCCCEEECCCEEEE
LKPSDVAVAVAVDGGLFTPVLKDSDSKSLSALSAEMKDLATRARDGKLAPHEYVGGSFAI
ECCCCEEEEEEECCCEECCEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCEEEH
SNLGMMGIENFDAVINPPHGAILAVGAGVKKPVVGADGELAVATVMSTTLSVDHRVIDGA
HHCCCCCCCCCCCEECCCCCEEEEECCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHH
LGAELLAAIKDNLENPMVMLA
HHHHHHHHHHHCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10796014; 11481430 [H]