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The map label for this gene is gph [C]

Identifier: 89053225

GI number: 89053225

Start: 704530

End: 705255

Strand: Direct

Name: gph [C]

Synonym: Jann_0734

Alternate gene names: 89053225

Gene position: 704530-705255 (Clockwise)

Preceding gene: 89053224

Following gene: 89053226

Centisome position: 16.32

GC content: 60.61

Gene sequence:

>726_bases
ATGGCTGGTCGACATTGGTCGAAACGGGTTCACGGGGACCCGCAGCCTCACAACGTTCCGCTCAAGGGATCACTGGTACT
CTTCGATGTTGACGGCACGCTTCTGGATGCCGGCGATCTCATTGCGCGAACTATGGTTGAGGCATTTCTGGCAGCGGGTG
AAACACCGCCCGCCGCGGACTTCGTGCAGCGTATCATCGGCCTGTCCCTCCCCGAAATGGTTTATAATCTGGGCGCACAT
CTGTCCGAGGATCGACGTCAGAAAATCCTTGCCGGCTACAGATTGCGCTACTTCGACCTGGTCGAGCAGGAGGAAACGCC
GGACGTCTTTCCCGGCGCAGCGCAAGCGCTGAACCGGCTTCGTGCGGCCGGTATGGTGCTTGGGATCACAACCGGCAAGG
CGCGGCGCAGCACGGAATTCATGTTGCGGATCAACAATTGGGACCGGTATTTTCACACCGTTCAATGCGCGGATGGAAAC
CCGTCGAAACCCGACCCCAAGATGATCCGACGCGCCATGGCCGAAACGGGCCGCACGCCATCTGAGACGATCCTGGTGGG
CGACTCGCGCTATGACATGCGCATGGCGAAGGCCGCAGGTGTCCGTGCTGTCGGCGTGGCGTGGGGGTACAATCAACCGG
TCGAATTGCTGACCGAAGGGGCCATGTTCATCGCCCGGGATTTTGAACATCTGACCGAGGCGCTGGTGCCCATGGCGGCG
GTCTGA

Upstream 100 bases:

>100_bases
ACCTACTGCCTATGTCCATATCAAATTCATCACACTTACGGCCCTCGGAAGGGCATGTTACTTTTGCGGCATCAGACGCA
TTTTCTGGTAATTGGGTAAC

Downstream 100 bases:

>100_bases
TCGTCCACAACGGCGCGTTCCGGCGGTATCGGCCCCCCTTCACGATCAGAGCGCAGGAATGTCGCACCCTGTCGCATTGC
CTTCGCGGCGCTGCGCTTTA

Product: HAD family hydrolase

Products: NA

Alternate protein names: PGP; PGPase [H]

Number of amino acids: Translated: 241; Mature: 240

Protein sequence:

>241_residues
MAGRHWSKRVHGDPQPHNVPLKGSLVLFDVDGTLLDAGDLIARTMVEAFLAAGETPPAADFVQRIIGLSLPEMVYNLGAH
LSEDRRQKILAGYRLRYFDLVEQEETPDVFPGAAQALNRLRAAGMVLGITTGKARRSTEFMLRINNWDRYFHTVQCADGN
PSKPDPKMIRRAMAETGRTPSETILVGDSRYDMRMAKAAGVRAVGVAWGYNQPVELLTEGAMFIARDFEHLTEALVPMAA
V

Sequences:

>Translated_241_residues
MAGRHWSKRVHGDPQPHNVPLKGSLVLFDVDGTLLDAGDLIARTMVEAFLAAGETPPAADFVQRIIGLSLPEMVYNLGAH
LSEDRRQKILAGYRLRYFDLVEQEETPDVFPGAAQALNRLRAAGMVLGITTGKARRSTEFMLRINNWDRYFHTVQCADGN
PSKPDPKMIRRAMAETGRTPSETILVGDSRYDMRMAKAAGVRAVGVAWGYNQPVELLTEGAMFIARDFEHLTEALVPMAA
V
>Mature_240_residues
AGRHWSKRVHGDPQPHNVPLKGSLVLFDVDGTLLDAGDLIARTMVEAFLAAGETPPAADFVQRIIGLSLPEMVYNLGAHL
SEDRRQKILAGYRLRYFDLVEQEETPDVFPGAAQALNRLRAAGMVLGITTGKARRSTEFMLRINNWDRYFHTVQCADGNP
SKPDPKMIRRAMAETGRTPSETILVGDSRYDMRMAKAAGVRAVGVAWGYNQPVELLTEGAMFIARDFEHLTEALVPMAAV

Specific function: Specifically catalyzes the dephosphorylation of 2- phosphoglycolate. Is involved in the dissimilation of the intracellular 2-phosphoglycolate formed during the DNA repair of 3'-phosphoglycolate ends, a major class of DNA lesions induced by oxidative stres

COG id: COG0546

COG function: function code R; Predicted phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family [H]

Homologues:

Organism=Escherichia coli, GI1789787, Length=208, Percent_Identity=29.3269230769231, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR005833
- InterPro:   IPR006346
- InterPro:   IPR023198 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: =3.1.3.18 [H]

Molecular weight: Translated: 26635; Mature: 26504

Theoretical pI: Translated: 7.17; Mature: 7.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGRHWSKRVHGDPQPHNVPLKGSLVLFDVDGTLLDAGDLIARTMVEAFLAAGETPPAAD
CCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCEECHHHHHHHHHHHHHHHCCCCCCHHH
FVQRIIGLSLPEMVYNLGAHLSEDRRQKILAGYRLRYFDLVEQEETPDVFPGAAQALNRL
HHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
RAAGMVLGITTGKARRSTEFMLRINNWDRYFHTVQCADGNPSKPDPKMIRRAMAETGRTP
HHCCEEEEEECCCCCCCEEEEEEECCCCCEEEEEEECCCCCCCCCHHHHHHHHHHCCCCC
SETILVGDSRYDMRMAKAAGVRAVGVAWGYNQPVELLTEGAMFIARDFEHLTEALVPMAA
CCEEEECCCCHHHHHHHHCCCEEEEEECCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHCC
V
C
>Mature Secondary Structure 
AGRHWSKRVHGDPQPHNVPLKGSLVLFDVDGTLLDAGDLIARTMVEAFLAAGETPPAAD
CCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCEECHHHHHHHHHHHHHHHCCCCCCHHH
FVQRIIGLSLPEMVYNLGAHLSEDRRQKILAGYRLRYFDLVEQEETPDVFPGAAQALNRL
HHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
RAAGMVLGITTGKARRSTEFMLRINNWDRYFHTVQCADGNPSKPDPKMIRRAMAETGRTP
HHCCEEEEEECCCCCCCEEEEEEECCCCCEEEEEEECCCCCCCCCHHHHHHHHHHCCCCC
SETILVGDSRYDMRMAKAAGVRAVGVAWGYNQPVELLTEGAMFIARDFEHLTEALVPMAA
CCEEEECCCCHHHHHHHHCCCEEEEEECCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHCC
V
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA