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The map label for this gene is aroA

Identifier: 89053199

GI number: 89053199

Start: 680451

End: 681803

Strand: Direct

Name: aroA

Synonym: Jann_0708

Alternate gene names: 89053199

Gene position: 680451-681803 (Clockwise)

Preceding gene: 89053198

Following gene: 89053200

Centisome position: 15.76

GC content: 67.18

Gene sequence:

>1353_bases
ATGTCAGCCCATGGTGATCCCAAACCGATGACGGCCCGCAAGGGCGGCGCGTTGACAGGCACGGCGGAGGTCCCCGGTGA
CAAGTCGATCTCCCACCGGTCGCTGATCTTTGGCGCAATGGCCGTTGGAGAGACCCGGGTGACGGGGTTGCTGGAAGGGC
AAGACGTCCTCGACACCGCCTCGGCGATGCGGGCGTTCGGGGCGGAGGTGGCGCGAGAGGACGATGGCACGTGGTCGATC
CACGGTGTGGGAGTCGGCGGTTTTGTGGAGCCGGACGACGTGCTGGATTGCGGCAATTCGGGCACCGGCGTGCGGCTCTT
GATGGGAGCGATGGCAACGACGCCGATTTCGGTGACGTTCACGGGCGACGCGTCCCTGCGGTCACGGCCCATGGCGCGGG
TCACGGACCCGTTGGCGCTGTTTGGCACCAGGGCCTACGGGCGTGCACAGGGGCGCTTGCCGATGACGTTGGTGGGCGCG
GAGGCCCCCATGCCGGTGCGCTATGCCACGCCGGTGCCGTCGGCGCAGGTGAAGTCCGCCGTTCTGTTGGCGGGGCTGAA
CGCGCCGGGTGAGACGGTCGTGATCGAGGCTGAGGCGACCCGCGACCACTCCGAGCGGATGCTGGCGGGGTTTGGGGCTC
AGATATCCACCGAGGTGACGCAGGAAGGCCGGGTGATCACGCTGACAGGCCAGCCGGAACTGACGCCGCAGGTTATTGCC
GTGCCGCGCGACCCAAGCTCTGCCGCGTTTCCGGTCTGTGCAGCCATCATCACCGAAGGCTCGGACGTGTTGGTGCCCAA
TATTGGGCTGAACCCGACGCGCGCCGGTCTGTTCGAGACGCTGCGCGATATGGGCGCGGATCTGACCTATGAGAACCCGC
GCGAAGAGGGGGGGGAGCCGGTGGCCGACCTGCGCGCGAAGTTTTCACCCGACATGAAGGGGATCGAGGTCCCGCCCGAG
CGCGCGGCGAGCATGATTGACGAATACCCGATCCTCTCGGTCGTCGCGGCCAACGCCGAGGGCACAACGGTCATGCGCGG
CGTCAAAGAGTTGCGGGTGAAGGAAAGCGACCGGATAGCCGCCATGGCGGACGGGCTGCGCCTGAACGGGATTGAAGTTG
AAGATGGCCCCGATTGGTGGATCGTCCATGGGCGCGGTCCCGGCGGCATGGCGGGCGGGGCGACGGTGGCCACGCATCTC
GATCATCGGATCGCGATGTCCTTCCTCTGTGCCGGGTTCGCCAGTCAGCAGGGCATCACGATTGACGACAGTGGCCCCAT
CGCCACGTCCTTCCCGATTTTCGAGCCGCTGATGCGGGACCTCGGGGCACGGCTGGACCGCGCAAACGCCTGA

Upstream 100 bases:

>100_bases
TGGAGCGGCGCGCCCGTCTTTGAGGTGTGGACGGCATGGGGAGGCGGGGCTATCAACCGCCTTGGAAATACTGGTCCGGT
CTATAGTCGAGAGTGCTCTG

Downstream 100 bases:

>100_bases
CTTGATCGGACGTGGCCCGGTGGGCTAACCTATGGGCAGGCTGGCGCGGCCTGCCGTCCGCATGGGGCATTGCCCCAAGG
GTGAATGCGCCAAAACGATC

Product: 3-phosphoshikimate 1-carboxyvinyltransferase

Products: NA

Alternate protein names: 5-enolpyruvylshikimate-3-phosphate synthase; EPSP synthase; EPSPS

Number of amino acids: Translated: 450; Mature: 449

Protein sequence:

>450_residues
MSAHGDPKPMTARKGGALTGTAEVPGDKSISHRSLIFGAMAVGETRVTGLLEGQDVLDTASAMRAFGAEVAREDDGTWSI
HGVGVGGFVEPDDVLDCGNSGTGVRLLMGAMATTPISVTFTGDASLRSRPMARVTDPLALFGTRAYGRAQGRLPMTLVGA
EAPMPVRYATPVPSAQVKSAVLLAGLNAPGETVVIEAEATRDHSERMLAGFGAQISTEVTQEGRVITLTGQPELTPQVIA
VPRDPSSAAFPVCAAIITEGSDVLVPNIGLNPTRAGLFETLRDMGADLTYENPREEGGEPVADLRAKFSPDMKGIEVPPE
RAASMIDEYPILSVVAANAEGTTVMRGVKELRVKESDRIAAMADGLRLNGIEVEDGPDWWIVHGRGPGGMAGGATVATHL
DHRIAMSFLCAGFASQQGITIDDSGPIATSFPIFEPLMRDLGARLDRANA

Sequences:

>Translated_450_residues
MSAHGDPKPMTARKGGALTGTAEVPGDKSISHRSLIFGAMAVGETRVTGLLEGQDVLDTASAMRAFGAEVAREDDGTWSI
HGVGVGGFVEPDDVLDCGNSGTGVRLLMGAMATTPISVTFTGDASLRSRPMARVTDPLALFGTRAYGRAQGRLPMTLVGA
EAPMPVRYATPVPSAQVKSAVLLAGLNAPGETVVIEAEATRDHSERMLAGFGAQISTEVTQEGRVITLTGQPELTPQVIA
VPRDPSSAAFPVCAAIITEGSDVLVPNIGLNPTRAGLFETLRDMGADLTYENPREEGGEPVADLRAKFSPDMKGIEVPPE
RAASMIDEYPILSVVAANAEGTTVMRGVKELRVKESDRIAAMADGLRLNGIEVEDGPDWWIVHGRGPGGMAGGATVATHL
DHRIAMSFLCAGFASQQGITIDDSGPIATSFPIFEPLMRDLGARLDRANA
>Mature_449_residues
SAHGDPKPMTARKGGALTGTAEVPGDKSISHRSLIFGAMAVGETRVTGLLEGQDVLDTASAMRAFGAEVAREDDGTWSIH
GVGVGGFVEPDDVLDCGNSGTGVRLLMGAMATTPISVTFTGDASLRSRPMARVTDPLALFGTRAYGRAQGRLPMTLVGAE
APMPVRYATPVPSAQVKSAVLLAGLNAPGETVVIEAEATRDHSERMLAGFGAQISTEVTQEGRVITLTGQPELTPQVIAV
PRDPSSAAFPVCAAIITEGSDVLVPNIGLNPTRAGLFETLRDMGADLTYENPREEGGEPVADLRAKFSPDMKGIEVPPER
AASMIDEYPILSVVAANAEGTTVMRGVKELRVKESDRIAAMADGLRLNGIEVEDGPDWWIVHGRGPGGMAGGATVATHLD
HRIAMSFLCAGFASQQGITIDDSGPIATSFPIFEPLMRDLGARLDRANA

Specific function: Aromatic amino acids biosynthesis; shikimate pathway; sixth step. [C]

COG id: COG0128

COG function: function code E; 5-enolpyruvylshikimate-3-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the EPSP synthase family

Homologues:

Organism=Escherichia coli, GI1787137, Length=432, Percent_Identity=30.5555555555556, Blast_Score=135, Evalue=7e-33,
Organism=Saccharomyces cerevisiae, GI6320332, Length=467, Percent_Identity=29.1220556745182, Blast_Score=138, Evalue=2e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AROA_JANSC (Q28UI7)

Other databases:

- EMBL:   CP000264
- RefSeq:   YP_508650.1
- ProteinModelPortal:   Q28UI7
- SMR:   Q28UI7
- STRING:   Q28UI7
- GeneID:   3933151
- GenomeReviews:   CP000264_GR
- KEGG:   jan:Jann_0708
- eggNOG:   COG0128
- HOGENOM:   HBG646626
- OMA:   IEVPPER
- ProtClustDB:   PRK02427
- BioCyc:   JSP290400:JANN_0708-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00210
- InterPro:   IPR001986
- InterPro:   IPR006264
- InterPro:   IPR023193
- InterPro:   IPR013792
- Gene3D:   G3DSA:3.65.10.10
- PIRSF:   PIRSF000505
- TIGRFAMs:   TIGR01356

Pfam domain/function: PF00275 EPSP_synthase; SSF55205 RNA3'_cycl/enolpyr_transf_A/B

EC number: =2.5.1.19

Molecular weight: Translated: 47051; Mature: 46920

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS00104 EPSP_SYNTHASE_1; PS00885 EPSP_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAHGDPKPMTARKGGALTGTAEVPGDKSISHRSLIFGAMAVGETRVTGLLEGQDVLDTA
CCCCCCCCCCCCCCCCCEEECCCCCCCCCCCHHHHEEEHHHCCCHHEEEEECCCHHHHHH
SAMRAFGAEVAREDDGTWSIHGVGVGGFVEPDDVLDCGNSGTGVRLLMGAMATTPISVTF
HHHHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHCCCCCCCHHHHHHHHHHCCCEEEEE
TGDASLRSRPMARVTDPLALFGTRAYGRAQGRLPMTLVGAEAPMPVRYATPVPSAQVKSA
ECCCHHHCCCHHHHCCCHHHHCCCCCCCCCCCCCEEEECCCCCCCEEEECCCCCHHHCCE
VLLAGLNAPGETVVIEAEATRDHSERMLAGFGAQISTEVTQEGRVITLTGQPELTPQVIA
EEEEECCCCCCEEEEEECCCCCHHHHHHHHCCCCHHHHHCCCCCEEEEECCCCCCCEEEE
VPRDPSSAAFPVCAAIITEGSDVLVPNIGLNPTRAGLFETLRDMGADLTYENPREEGGEP
ECCCCCCCHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHCCCC
VADLRAKFSPDMKGIEVPPERAASMIDEYPILSVVAANAEGTTVMRGVKELRVKESDRIA
HHHHHHHCCCCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCEE
AMADGLRLNGIEVEDGPDWWIVHGRGPGGMAGGATVATHLDHRIAMSFLCAGFASQQGIT
EHHCCEEECCEEECCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEE
IDDSGPIATSFPIFEPLMRDLGARLDRANA
ECCCCCCEECCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SAHGDPKPMTARKGGALTGTAEVPGDKSISHRSLIFGAMAVGETRVTGLLEGQDVLDTA
CCCCCCCCCCCCCCCCEEECCCCCCCCCCCHHHHEEEHHHCCCHHEEEEECCCHHHHHH
SAMRAFGAEVAREDDGTWSIHGVGVGGFVEPDDVLDCGNSGTGVRLLMGAMATTPISVTF
HHHHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHCCCCCCCHHHHHHHHHHCCCEEEEE
TGDASLRSRPMARVTDPLALFGTRAYGRAQGRLPMTLVGAEAPMPVRYATPVPSAQVKSA
ECCCHHHCCCHHHHCCCHHHHCCCCCCCCCCCCCEEEECCCCCCCEEEECCCCCHHHCCE
VLLAGLNAPGETVVIEAEATRDHSERMLAGFGAQISTEVTQEGRVITLTGQPELTPQVIA
EEEEECCCCCCEEEEEECCCCCHHHHHHHHCCCCHHHHHCCCCCEEEEECCCCCCCEEEE
VPRDPSSAAFPVCAAIITEGSDVLVPNIGLNPTRAGLFETLRDMGADLTYENPREEGGEP
ECCCCCCCHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHCCCC
VADLRAKFSPDMKGIEVPPERAASMIDEYPILSVVAANAEGTTVMRGVKELRVKESDRIA
HHHHHHHCCCCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCEE
AMADGLRLNGIEVEDGPDWWIVHGRGPGGMAGGATVATHLDHRIAMSFLCAGFASQQGIT
EHHCCEEECCEEECCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEE
IDDSGPIATSFPIFEPLMRDLGARLDRANA
ECCCCCCEECCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA