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The map label for this gene is trmB

Identifier: 89053198

GI number: 89053198

Start: 679441

End: 680265

Strand: Direct

Name: trmB

Synonym: Jann_0707

Alternate gene names: 89053198

Gene position: 679441-680265 (Clockwise)

Preceding gene: 89053197

Following gene: 89053199

Centisome position: 15.74

GC content: 63.39

Gene sequence:

>825_bases
ATGTGCCGGGCTTTTCCGTTGGCCGATCTCCAGCTCGATCCCGGTTCGGACGACGCCGCGTGCGCCGCAACCCTTGAGGT
GGCGCGAGGATCAAGATACTCCGCCGCCACCATGACTGATCCCCATCCCAACCGCCCGCATCGCAACTTCTATGGCCGCC
GCAAGGGCCATGACCTTCGTGACAGCCAGGAGCGGTATCTGGCCGAGGATTTGGCGAAACTGTCGCCGGGCGCTGTGGAT
TGGGATGTGAACCCCGACCGCACGCCGCTGGATCTGAAAGAACGTTTCGGTGGCAAGGACATCTGGCTGGAGGTGGGTTT
TGGCGGCGGGGAGCACTTGGTGCATCAGGCGGCCAGCAATCCGGACGTCGCGATCATCGGATGCGAGCCTTACATCAACG
GCGTTGCCATGCTGCTCGGCAAGATCCGCGCGGCGGGGGTGGGCAATCTCGCCGTTTATCCCGGCGATGCGCGCGATTTG
TTTGATGTGCTGCCAAAGGGATCAGTGGCCAAGGCGTTTCTGCTCTACCCTGATCCTTGGCCGAAAGCCCGCCACCATCG
CCGTCGCTTCGTGACGACTGAACACCTGGAGCCGTTGCACCGCGCCATGAGGCCGGGTGCAGAATTCCGCGTGGCCACCG
ACATCGAGGATTACGTGCGCCAGACCATGGAGGAGGTCCCGAAGGCGGGGTTCGAGTGGCTGGCGGAGGCCGCGTCGGAT
TGGCGGGAGCCGTGGTGTGACTGGGTGTCAACGCGGTATGAGCAGAAGGCGCTGCGCGAGGGCCGGGTGCCCCATTACAT
GACGTTTCAAAAGCGCTCACTTTAG

Upstream 100 bases:

>100_bases
GACGGATCTGGCGGACGCGATCAAGGCGGCGCTCTAGGGCCGACTATCGCCACTTTGGCCTACAGTGATAAACTCCGGCT
CTGACCCGTTTTGATCCGCC

Downstream 100 bases:

>100_bases
AGCGTTTTGGGGGCCAGCCCCCTCTGCGATCTTTTTGCGAGCAAAAAGACGCATACCCCCGGAGTTGTAGGGCCCAGATG
AAAGATGGAGCGGCGCGCCC

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase [H]

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MCRAFPLADLQLDPGSDDAACAATLEVARGSRYSAATMTDPHPNRPHRNFYGRRKGHDLRDSQERYLAEDLAKLSPGAVD
WDVNPDRTPLDLKERFGGKDIWLEVGFGGGEHLVHQAASNPDVAIIGCEPYINGVAMLLGKIRAAGVGNLAVYPGDARDL
FDVLPKGSVAKAFLLYPDPWPKARHHRRRFVTTEHLEPLHRAMRPGAEFRVATDIEDYVRQTMEEVPKAGFEWLAEAASD
WREPWCDWVSTRYEQKALREGRVPHYMTFQKRSL

Sequences:

>Translated_274_residues
MCRAFPLADLQLDPGSDDAACAATLEVARGSRYSAATMTDPHPNRPHRNFYGRRKGHDLRDSQERYLAEDLAKLSPGAVD
WDVNPDRTPLDLKERFGGKDIWLEVGFGGGEHLVHQAASNPDVAIIGCEPYINGVAMLLGKIRAAGVGNLAVYPGDARDL
FDVLPKGSVAKAFLLYPDPWPKARHHRRRFVTTEHLEPLHRAMRPGAEFRVATDIEDYVRQTMEEVPKAGFEWLAEAASD
WREPWCDWVSTRYEQKALREGRVPHYMTFQKRSL
>Mature_274_residues
MCRAFPLADLQLDPGSDDAACAATLEVARGSRYSAATMTDPHPNRPHRNFYGRRKGHDLRDSQERYLAEDLAKLSPGAVD
WDVNPDRTPLDLKERFGGKDIWLEVGFGGGEHLVHQAASNPDVAIIGCEPYINGVAMLLGKIRAAGVGNLAVYPGDARDL
FDVLPKGSVAKAFLLYPDPWPKARHHRRRFVTTEHLEPLHRAMRPGAEFRVATDIEDYVRQTMEEVPKAGFEWLAEAASD
WREPWCDWVSTRYEQKALREGRVPHYMTFQKRSL

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA [H]

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family [H]

Homologues:

Organism=Escherichia coli, GI1789330, Length=175, Percent_Identity=33.7142857142857, Blast_Score=84, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003358 [H]

Pfam domain/function: PF02390 Methyltransf_4 [H]

EC number: =2.1.1.33 [H]

Molecular weight: Translated: 30919; Mature: 30919

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCRAFPLADLQLDPGSDDAACAATLEVARGSRYSAATMTDPHPNRPHRNFYGRRKGHDLR
CCCCCCCCCEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
DSQERYLAEDLAKLSPGAVDWDVNPDRTPLDLKERFGGKDIWLEVGFGGGEHLVHQAASN
HHHHHHHHHHHHHCCCCCEECCCCCCCCCCHHHHHCCCCEEEEEECCCCHHHHHHHHCCC
PDVAIIGCEPYINGVAMLLGKIRAAGVGNLAVYPGDARDLFDVLPKGSVAKAFLLYPDPW
CCEEEEECCHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHCCCCCCCEEEEECCCCC
PKARHHRRRFVTTEHLEPLHRAMRPGAEFRVATDIEDYVRQTMEEVPKAGFEWLAEAASD
CHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHH
WREPWCDWVSTRYEQKALREGRVPHYMTFQKRSL
HHHHHHHHHHHHHHHHHHHHCCCCCEECCHHCCC
>Mature Secondary Structure
MCRAFPLADLQLDPGSDDAACAATLEVARGSRYSAATMTDPHPNRPHRNFYGRRKGHDLR
CCCCCCCCCEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
DSQERYLAEDLAKLSPGAVDWDVNPDRTPLDLKERFGGKDIWLEVGFGGGEHLVHQAASN
HHHHHHHHHHHHHCCCCCEECCCCCCCCCCHHHHHCCCCEEEEEECCCCHHHHHHHHCCC
PDVAIIGCEPYINGVAMLLGKIRAAGVGNLAVYPGDARDLFDVLPKGSVAKAFLLYPDPW
CCEEEEECCHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHCCCCCCCEEEEECCCCC
PKARHHRRRFVTTEHLEPLHRAMRPGAEFRVATDIEDYVRQTMEEVPKAGFEWLAEAASD
CHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHH
WREPWCDWVSTRYEQKALREGRVPHYMTFQKRSL
HHHHHHHHHHHHHHHHHHHHCCCCCEECCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA