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The map label for this gene is virB4-2 [H]

Identifier: 88657785

GI number: 88657785

Start: 1071112

End: 1073487

Strand: Reverse

Name: virB4-2 [H]

Synonym: ECH_1041

Alternate gene names: 88657785

Gene position: 1073487-1071112 (Counterclockwise)

Preceding gene: 88657979

Following gene: 88658429

Centisome position: 91.26

GC content: 30.89

Gene sequence:

>2376_bases
ATGTCTTTTGTGAAAGAAATGATCGGCCATTCTTCTGATATGAATAATTTTTCTAGAAAAAGACGAGATAATACCTCTAG
TAAAGGAGATTTTATTCCTGCAGCTTGTCATTATGATGAGAATACAATATTGAATAAAGATGGTGAACTTGTACAAATAA
TAAAGATAGAGGATTATGTACTTACTCATTATGTTAATGATAAAGATTTAAGAACAGTAGTGCGTAATAGTATAGTTAAT
AGTGTTGAAGTTCCAGAAGTTTCTTTTTGGATTTATACTGTAAGGAAACCACATAAGTTTGATTTTGCAAGAAAAAGTAT
AAACGATGTTTCTGATGCTTTAGGTAGTGCTCATCTTAATAATATAGGTCAACGTGTGACTTATATTAATGAGTTATATA
TAGCAGTGGTTACTAATCACTTACCTGAAAGTATGAAAGGAGTGTTAGGTGCTCTGTCATTCTCCTATGTAAAAAATAAG
CATAAAGATTTTTTAAAAAATAAAATAGACAGATTAAATAAGGTCACTGCAAGTATTTTAGAAAATTTGAAAAAGTTTGA
AGTCAGAAAGTTAGGATTGATAGTAATTGATAAAGAAAAAGTAAGATCGGAGTTGATAGAGTTTTTGTATTATTTAACTA
TGATGCATCATAAAGAATGTTTCCTTGATATGGTAGATATTTCTGGTATATGTAGTCATTGTAGTATTAGTATGGGATTT
AATACATTTAAAATTTCATGTGATAATAACCAAAGATTTGGTGCAATATTAGCAATTAAAGATTATCAAGATTCTCCATT
AGATGCGGTAGATGAATGTCTACAGCAGGATTATGGATTTATCGTAGTTGAAATTATAAAGTTTGCAAAAAGTAAGAATG
CATTAAAGCTTTTTCAAAAGCAAGCTACATTTTTAGAATGTAGCAATGATTTTCAGTTGAGAAAGTTATCCAATATAGAT
GATTTCGTATCAGTTGATCCAAACTCTAATTTAAGTTTCTGCGAACGCAAAATAAACTTTGTAATAATGTCAGATACTTT
ACCACAGCTTCATAATAATATAGATAGGGCTGTTAATTCATTGTCTTCACTTGGTATTATTTGTGTCAGGTGTGATTTGA
GCATGGAAGATGATTTTTGGGCACATTTACCTGGCAATTTTTCTTATATTTTAAACTTTAGGTATACGTTAATAAAATAT
GCTTGTGCATTTTCGTTGTTGCATTATTTCCCTTCAGGAGCACTTCAAGGAAACAAGTGGGGACAAGCAATTACTATGTT
TTTTTCAAATAAGGGTAAACCTTATTTCTTTAGTTTTCATGTGTTTGATAAAGGACACACTTTAATGGTTGGTAGTCCTC
AATCTTCAGTTACTATGTTACTGAACTTTTTATTGTCAGAATCTATGCAGTTGAATGCACGAATTGTCTTGCTAGATTAT
ACTGGTAAGTCTATTGTTTTTGTTAAGGCTATGGGTGGTCAGTATTATAGAGCAGACCATAGGCGTGATTATCAGGAAAT
GTCGTTTAATTTCTTTCAAGTTGAAGATACTGCACTTAATCGTAGAATCGTTACTGGTGTTTTGCAAAGAATGTTGAATG
TTAAAAACATAACTGAGGAAGTTAATAGTGCAATAGATAGAATAGTTAATGATCTTTTTACGTTGCCTCTTGAGTCTAGA
ACTATAAATAGTATTGCTGACCATGTCAGTACACTGGGTACCAATGCTAGTCAATGGTTAAATAATGGGGAGTTTGCGCA
TTTACTAAAGGAAGATGCTAATATTGATTGGGCAGCAAAAGTTTTAGGGTTGAATATTGGTATTTTGTTCTCTAAACCTC
AATGTGCTTCTGTTATTGTTTATTACTTTTTGCATGCTTTAATTAATTATCTTGATGGGTCTCCTACGGTTTTAGTAATA
GATGAAGCATGGATTTTGGATTATGTTTTTACTAGTGATCAGGAATTTGATGAGTGGATTGAAATGATGAACAAATTAAA
TGTTGTTGTTGTATTTGCTGGTGAAAACATTCCAGCTATTATTTCCAGTAATATCATTTGTAGGTTTAATCAGCATGTTG
AAACACAAGTTTTCATGCCAAATTCAGTATCAACTAATAAAATGTATATGAGGGCATTTAATCTATCAAAGTCAGAATGT
AATACTATGTTTCAAATGCCATCTCAGGAAGGATATTTTTTCGTAAAGCAGGATAATGATTCAGTAGTATTGTCTTTCAA
TTTGCCAAATATACCAGAAACTAATGTTCTTTCTGCTAATAAGAATACAATTCGGTATATGTATGAATCTATTAGTAGTC
ATGGGGATAATGTAAGAGAATGGCTGCCTGCATTTTATAAGAAATGTGGAGCTTAA

Upstream 100 bases:

>100_bases
TTAGATAATGATGATTAAAGCAGATATTATCCTATATAGATGTTAATTTTACTTATGGATATTTATATAAATGTTATAGG
GTTAAATAAGGGTTTCCTGT

Downstream 100 bases:

>100_bases
AATTTTACACTGTATGAAATTTATGCAATGTAAATGGACTTAACATAATATTAGTAACATTTGTAGAAGTTGTTGATCAC
AAGCTTGTTTGTGATAAAAA

Product: type IV secretion system protein VirB4

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 791; Mature: 790

Protein sequence:

>791_residues
MSFVKEMIGHSSDMNNFSRKRRDNTSSKGDFIPAACHYDENTILNKDGELVQIIKIEDYVLTHYVNDKDLRTVVRNSIVN
SVEVPEVSFWIYTVRKPHKFDFARKSINDVSDALGSAHLNNIGQRVTYINELYIAVVTNHLPESMKGVLGALSFSYVKNK
HKDFLKNKIDRLNKVTASILENLKKFEVRKLGLIVIDKEKVRSELIEFLYYLTMMHHKECFLDMVDISGICSHCSISMGF
NTFKISCDNNQRFGAILAIKDYQDSPLDAVDECLQQDYGFIVVEIIKFAKSKNALKLFQKQATFLECSNDFQLRKLSNID
DFVSVDPNSNLSFCERKINFVIMSDTLPQLHNNIDRAVNSLSSLGIICVRCDLSMEDDFWAHLPGNFSYILNFRYTLIKY
ACAFSLLHYFPSGALQGNKWGQAITMFFSNKGKPYFFSFHVFDKGHTLMVGSPQSSVTMLLNFLLSESMQLNARIVLLDY
TGKSIVFVKAMGGQYYRADHRRDYQEMSFNFFQVEDTALNRRIVTGVLQRMLNVKNITEEVNSAIDRIVNDLFTLPLESR
TINSIADHVSTLGTNASQWLNNGEFAHLLKEDANIDWAAKVLGLNIGILFSKPQCASVIVYYFLHALINYLDGSPTVLVI
DEAWILDYVFTSDQEFDEWIEMMNKLNVVVVFAGENIPAIISSNIICRFNQHVETQVFMPNSVSTNKMYMRAFNLSKSEC
NTMFQMPSQEGYFFVKQDNDSVVLSFNLPNIPETNVLSANKNTIRYMYESISSHGDNVREWLPAFYKKCGA

Sequences:

>Translated_791_residues
MSFVKEMIGHSSDMNNFSRKRRDNTSSKGDFIPAACHYDENTILNKDGELVQIIKIEDYVLTHYVNDKDLRTVVRNSIVN
SVEVPEVSFWIYTVRKPHKFDFARKSINDVSDALGSAHLNNIGQRVTYINELYIAVVTNHLPESMKGVLGALSFSYVKNK
HKDFLKNKIDRLNKVTASILENLKKFEVRKLGLIVIDKEKVRSELIEFLYYLTMMHHKECFLDMVDISGICSHCSISMGF
NTFKISCDNNQRFGAILAIKDYQDSPLDAVDECLQQDYGFIVVEIIKFAKSKNALKLFQKQATFLECSNDFQLRKLSNID
DFVSVDPNSNLSFCERKINFVIMSDTLPQLHNNIDRAVNSLSSLGIICVRCDLSMEDDFWAHLPGNFSYILNFRYTLIKY
ACAFSLLHYFPSGALQGNKWGQAITMFFSNKGKPYFFSFHVFDKGHTLMVGSPQSSVTMLLNFLLSESMQLNARIVLLDY
TGKSIVFVKAMGGQYYRADHRRDYQEMSFNFFQVEDTALNRRIVTGVLQRMLNVKNITEEVNSAIDRIVNDLFTLPLESR
TINSIADHVSTLGTNASQWLNNGEFAHLLKEDANIDWAAKVLGLNIGILFSKPQCASVIVYYFLHALINYLDGSPTVLVI
DEAWILDYVFTSDQEFDEWIEMMNKLNVVVVFAGENIPAIISSNIICRFNQHVETQVFMPNSVSTNKMYMRAFNLSKSEC
NTMFQMPSQEGYFFVKQDNDSVVLSFNLPNIPETNVLSANKNTIRYMYESISSHGDNVREWLPAFYKKCGA
>Mature_790_residues
SFVKEMIGHSSDMNNFSRKRRDNTSSKGDFIPAACHYDENTILNKDGELVQIIKIEDYVLTHYVNDKDLRTVVRNSIVNS
VEVPEVSFWIYTVRKPHKFDFARKSINDVSDALGSAHLNNIGQRVTYINELYIAVVTNHLPESMKGVLGALSFSYVKNKH
KDFLKNKIDRLNKVTASILENLKKFEVRKLGLIVIDKEKVRSELIEFLYYLTMMHHKECFLDMVDISGICSHCSISMGFN
TFKISCDNNQRFGAILAIKDYQDSPLDAVDECLQQDYGFIVVEIIKFAKSKNALKLFQKQATFLECSNDFQLRKLSNIDD
FVSVDPNSNLSFCERKINFVIMSDTLPQLHNNIDRAVNSLSSLGIICVRCDLSMEDDFWAHLPGNFSYILNFRYTLIKYA
CAFSLLHYFPSGALQGNKWGQAITMFFSNKGKPYFFSFHVFDKGHTLMVGSPQSSVTMLLNFLLSESMQLNARIVLLDYT
GKSIVFVKAMGGQYYRADHRRDYQEMSFNFFQVEDTALNRRIVTGVLQRMLNVKNITEEVNSAIDRIVNDLFTLPLESRT
INSIADHVSTLGTNASQWLNNGEFAHLLKEDANIDWAAKVLGLNIGILFSKPQCASVIVYYFLHALINYLDGSPTVLVID
EAWILDYVFTSDQEFDEWIEMMNKLNVVVVFAGENIPAIISSNIICRFNQHVETQVFMPNSVSTNKMYMRAFNLSKSECN
TMFQMPSQEGYFFVKQDNDSVVLSFNLPNIPETNVLSANKNTIRYMYESISSHGDNVREWLPAFYKKCGA

Specific function: The type IV secretion system VirB/VirD4 is a major virulence determinant for subversion of human endothelial cell (HEC) function. VirB-dependent changes of HEC include massive cytoskeletal rearrangements, a proinflammatory activation by nuclear factor NF-

COG id: COG3451

COG function: function code U; Type IV secretory pathway, VirB4 components

Gene ontology:

Cell location: Cell inner membrane (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trbE/virB4 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR004346
- InterPro:   IPR018145 [H]

Pfam domain/function: PF03135 CagE_TrbE_VirB [H]

EC number: NA

Molecular weight: Translated: 90489; Mature: 90358

Theoretical pI: Translated: 6.70; Mature: 6.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFVKEMIGHSSDMNNFSRKRRDNTSSKGDFIPAACHYDENTILNKDGELVQIIKIEDYV
CCHHHHHHCCCCCCHHHHHHHCCCCCCCCCCCCEEEECCCCCEECCCCCEEEEEEEEEEE
LTHYVNDKDLRTVVRNSIVNSVEVPEVSFWIYTVRKPHKFDFARKSINDVSDALGSAHLN
EEEECCCHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
NIGQRVTYINELYIAVVTNHLPESMKGVLGALSFSYVKNKHKDFLKNKIDRLNKVTASIL
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ENLKKFEVRKLGLIVIDKEKVRSELIEFLYYLTMMHHKECFLDMVDISGICSHCSISMGF
HHHHHHHHHHCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEECC
NTFKISCDNNQRFGAILAIKDYQDSPLDAVDECLQQDYGFIVVEIIKFAKSKNALKLFQK
EEEEEEECCCCCEEEEEEEECCCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHH
QATFLECSNDFQLRKLSNIDDFVSVDPNSNLSFCERKINFVIMSDTLPQLHNNIDRAVNS
HHHHEECCCCCCCHHHCCCCCEEEECCCCCHHHHHHEEEEEEEECCHHHHHHHHHHHHHH
LSSLGIICVRCDLSMEDDFWAHLPGNFSYILNFRYTLIKYACAFSLLHYFPSGALQGNKW
HHHCCEEEEEECCCCCCCCEEECCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
GQAITMFFSNKGKPYFFSFHVFDKGHTLMVGSPQSSVTMLLNFLLSESMQLNARIVLLDY
CCEEEEEECCCCCEEEEEEEEECCCCEEEEECCCHHHHHHHHHHHCCCCCCCEEEEEEEE
TGKSIVFVKAMGGQYYRADHRRDYQEMSFNFFQVEDTALNRRIVTGVLQRMLNVKNITEE
CCCEEEEEEECCCCEEECHHHCCHHHHCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHH
VNSAIDRIVNDLFTLPLESRTINSIADHVSTLGTNASQWLNNGEFAHLLKEDANIDWAAK
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHCCCCCHHHH
VLGLNIGILFSKPQCASVIVYYFLHALINYLDGSPTVLVIDEAWILDYVFTSDQEFDEWI
HHCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCEEEEEEECCCCCHHHHH
EMMNKLNVVVVFAGENIPAIISSNIICRFNQHVETQVFMPNSVSTNKMYMRAFNLSKSEC
HHHCCCCEEEEECCCCCCHHHCCCEEEEECCCCEEEEECCCCCCCCCEEEEEECCCHHHH
NTMFQMPSQEGYFFVKQDNDSVVLSFNLPNIPETNVLSANKNTIRYMYESISSHGDNVRE
HHHHCCCCCCCEEEEEECCCCEEEEECCCCCCCCCEECCCCHHHHHHHHHHHCCCCHHHH
WLPAFYKKCGA
HHHHHHHHHCC
>Mature Secondary Structure 
SFVKEMIGHSSDMNNFSRKRRDNTSSKGDFIPAACHYDENTILNKDGELVQIIKIEDYV
CHHHHHHCCCCCCHHHHHHHCCCCCCCCCCCCEEEECCCCCEECCCCCEEEEEEEEEEE
LTHYVNDKDLRTVVRNSIVNSVEVPEVSFWIYTVRKPHKFDFARKSINDVSDALGSAHLN
EEEECCCHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
NIGQRVTYINELYIAVVTNHLPESMKGVLGALSFSYVKNKHKDFLKNKIDRLNKVTASIL
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ENLKKFEVRKLGLIVIDKEKVRSELIEFLYYLTMMHHKECFLDMVDISGICSHCSISMGF
HHHHHHHHHHCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEECC
NTFKISCDNNQRFGAILAIKDYQDSPLDAVDECLQQDYGFIVVEIIKFAKSKNALKLFQK
EEEEEEECCCCCEEEEEEEECCCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHH
QATFLECSNDFQLRKLSNIDDFVSVDPNSNLSFCERKINFVIMSDTLPQLHNNIDRAVNS
HHHHEECCCCCCCHHHCCCCCEEEECCCCCHHHHHHEEEEEEEECCHHHHHHHHHHHHHH
LSSLGIICVRCDLSMEDDFWAHLPGNFSYILNFRYTLIKYACAFSLLHYFPSGALQGNKW
HHHCCEEEEEECCCCCCCCEEECCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
GQAITMFFSNKGKPYFFSFHVFDKGHTLMVGSPQSSVTMLLNFLLSESMQLNARIVLLDY
CCEEEEEECCCCCEEEEEEEEECCCCEEEEECCCHHHHHHHHHHHCCCCCCCEEEEEEEE
TGKSIVFVKAMGGQYYRADHRRDYQEMSFNFFQVEDTALNRRIVTGVLQRMLNVKNITEE
CCCEEEEEEECCCCEEECHHHCCHHHHCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHH
VNSAIDRIVNDLFTLPLESRTINSIADHVSTLGTNASQWLNNGEFAHLLKEDANIDWAAK
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHCCCCCHHHH
VLGLNIGILFSKPQCASVIVYYFLHALINYLDGSPTVLVIDEAWILDYVFTSDQEFDEWI
HHCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCEEEEEEECCCCCHHHHH
EMMNKLNVVVVFAGENIPAIISSNIICRFNQHVETQVFMPNSVSTNKMYMRAFNLSKSEC
HHHCCCCEEEEECCCCCCHHHCCCEEEEECCCCEEEEECCCCCCCCCEEEEEECCCHHHH
NTMFQMPSQEGYFFVKQDNDSVVLSFNLPNIPETNVLSANKNTIRYMYESISSHGDNVRE
HHHHCCCCCCCEEEEEECCCCEEEEECCCCCCCCCEECCCCHHHHHHHHHHHCCCCHHHH
WLPAFYKKCGA
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10749166; 10882236; 7494028 [H]