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The map label for this gene is xthA [H]

Identifier: 88607806

GI number: 88607806

Start: 499228

End: 499785

Strand: Direct

Name: xthA [H]

Synonym: APH_0471

Alternate gene names: 88607806

Gene position: 499228-499785 (Clockwise)

Preceding gene: 88606922

Following gene: 88607336

Centisome position: 33.93

GC content: 40.68

Gene sequence:

>558_bases
TTGGAAAGCGCAGGAAGTTATTCATCTGAGGAATCAAGATATATAGAGTGTACACTGGAGTGTACTGGGAATAAGAAAAT
AAGAGTAGTGAGCGTCTACGTTCCCAACGGGCAGGAGGTAGAATCTGAGACGTTTTTCTATAAATTGAAATTCCTGGAAC
ATCTGAAGGACAGATTGTTAAACATCATGAAAACAGAGGACTTCCTCATTGCTGGGGGTGACTTCAATGTTGCACCTGAA
GAGATAGACGTGCACGATCCTAAAGCTCTTGATGGCAGGCTATGCTTTCATATTTTAGAGCGTGCTAAATTCAGAGAAAT
TCTAAATAACGGTATAGTGGATATTTTTCGCACTTTTGTGGGTATTGATAGAAAAGAGTTTAGTTGGTGGAATTACAGAG
AAGGCGGATGGCAGAATAATAGAGGTCTTAGAATAGACGCTCTGCTATCTTCTCCTCAAATCGCTGATAAAGTTCTAGAT
TGTTCTATACTATCGAAGGTTAGAGGTTGGGATACTCCTTCGGATCATGCTCCGGTTATGGGCGACATAGATGTCTAA

Upstream 100 bases:

>100_bases
TAATCTCAGCTTTCATCTACATCACCTTAAAAATAAGCAGAAGTGTCAGTATTGTTCTTGCGCACCTGCATCAGCGCTGT
CAGAGCCAGAAACAAAGGAG

Downstream 100 bases:

>100_bases
TGTGGGTTCATGTGCTTAATTCTTGGTGCTATTTGGGGTTTTGATACGTATAAGTTGTGGGGTGTCTTGACTCTCTTATG
AGGTGTTTTTAAGGTATTGC

Product: exodeoxyribonuclease III, N-terminal truncation

Products: NA

Alternate protein names: EXO III; Exonuclease III [H]

Number of amino acids: Translated: 185; Mature: 185

Protein sequence:

>185_residues
MESAGSYSSEESRYIECTLECTGNKKIRVVSVYVPNGQEVESETFFYKLKFLEHLKDRLLNIMKTEDFLIAGGDFNVAPE
EIDVHDPKALDGRLCFHILERAKFREILNNGIVDIFRTFVGIDRKEFSWWNYREGGWQNNRGLRIDALLSSPQIADKVLD
CSILSKVRGWDTPSDHAPVMGDIDV

Sequences:

>Translated_185_residues
MESAGSYSSEESRYIECTLECTGNKKIRVVSVYVPNGQEVESETFFYKLKFLEHLKDRLLNIMKTEDFLIAGGDFNVAPE
EIDVHDPKALDGRLCFHILERAKFREILNNGIVDIFRTFVGIDRKEFSWWNYREGGWQNNRGLRIDALLSSPQIADKVLD
CSILSKVRGWDTPSDHAPVMGDIDV
>Mature_185_residues
MESAGSYSSEESRYIECTLECTGNKKIRVVSVYVPNGQEVESETFFYKLKFLEHLKDRLLNIMKTEDFLIAGGDFNVAPE
EIDVHDPKALDGRLCFHILERAKFREILNNGIVDIFRTFVGIDRKEFSWWNYREGGWQNNRGLRIDALLSSPQIADKVLD
CSILSKVRGWDTPSDHAPVMGDIDV

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction [H]

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Escherichia coli, GI1788046, Length=160, Percent_Identity=31.875, Blast_Score=85, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71989536, Length=164, Percent_Identity=34.7560975609756, Blast_Score=74, Evalue=3e-14,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 21208; Mature: 21208

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESAGSYSSEESRYIECTLECTGNKKIRVVSVYVPNGQEVESETFFYKLKFLEHLKDRLL
CCCCCCCCCCCCEEEEEEEEECCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHH
NIMKTEDFLIAGGDFNVAPEEIDVHDPKALDGRLCFHILERAKFREILNNGIVDIFRTFV
HHHCCCCEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
GIDRKEFSWWNYREGGWQNNRGLRIDALLSSPQIADKVLDCSILSKVRGWDTPSDHAPVM
CCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCE
GDIDV
ECCCC
>Mature Secondary Structure
MESAGSYSSEESRYIECTLECTGNKKIRVVSVYVPNGQEVESETFFYKLKFLEHLKDRLL
CCCCCCCCCCCCEEEEEEEEECCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHH
NIMKTEDFLIAGGDFNVAPEEIDVHDPKALDGRLCFHILERAKFREILNNGIVDIFRTFV
HHHCCCCEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
GIDRKEFSWWNYREGGWQNNRGLRIDALLSSPQIADKVLDCSILSKVRGWDTPSDHAPVM
CCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCE
GDIDV
ECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]