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The map label for this gene is fabI [H]

Identifier: 88607336

GI number: 88607336

Start: 500764

End: 501591

Strand: Direct

Name: fabI [H]

Synonym: APH_0473

Alternate gene names: 88607336

Gene position: 500764-501591 (Clockwise)

Preceding gene: 88607806

Following gene: 88606838

Centisome position: 34.04

GC content: 47.1

Gene sequence:

>828_bases
GTGCGTACCGGCATGCTCATGGAAGGAAAAAAGGGAGTCATCATTGGTGTAGCTAATGATAAGTCCTTGGCGTGGGGCAT
CGCGAAAGCTGTGTGTGCACAGGGTGCCGAGGTTGCTCTTACATATCTTTCGGAGACTTTTAAGAAAAGGGTAGATCCCC
TTGCGGAGTCTCTAGGTGTTAAGTTGACGGTTCCGTGTGATGTATCAGATGCGGAATCTGTAGACAATATGTTCAAAGTT
CTTGCTGAGGAGTGGGGTAGTCTGGATTTTGTGGTCCACGCGGTTGCATTTTCCGATAAGAACGAGCTCAAGGGAAGGTA
TGTTGATACTTCTCTGGGTAACTTCCTGACGTCGATGCATATATCGTGTTATTCTTTTACTTACATTGCGAGTAAAGCTG
AGCCACTGATGACCAATGGTGGCAGTATTTTGACTCTCTCTTATTACGGTGCTGAGAAGGTAGTGCCCCATTACAATGTG
ATGGGCGTGTGCAAGGCGGCCCTCGAGGCAAGTGTTAAATATTTGGCGGTTGATTTGGGTAAGCAGCAGATAAGAGTTAA
TGCTATTTCTGCGGGGCCTGTTAGAACTTTGGCGTCTTCTGGAATTAGTGATTTTCATTATATACTGACGTGGAATAAGT
ATAATTCGCCGTTGAGGCGCAATACTACTCTTGATGATGTCGGGGGGGCTGCGTTATATCTGCTGAGTGATTTGGGTAGA
GGGACAACTGGTGAGACTGTGCATGTGGATTGTGGGTACCATGTGGTGGGGATGAAATCGGTTGATGCTCCTGACATAAG
CCGGGTGAAAGGCGATCATAGCGTGTGA

Upstream 100 bases:

>100_bases
GTTAGAGCGCAAGCCTGTCACGTTTGAGGTCGCGAGTTCGAGTCTCGTCACTCCCGCGTTGTGTTTGTAGTAGGTATTGT
TAGAAGTTTACGGGTGTTTT

Downstream 100 bases:

>100_bases
GCGTTGGTAGCCTTGCAGTAAAAGTTTATAAGTGATTTTCCGTTTAGCGTGATGCTTGTTTTGTCTCTCGCATAGGGGGA
GTGTGCTTTGAGATTGTGGT

Product: enoyl-(acyl-carrier-protein) reductase

Products: NA

Alternate protein names: NADH-dependent enoyl-ACP reductase 1 [H]

Number of amino acids: Translated: 275; Mature: 275

Protein sequence:

>275_residues
MRTGMLMEGKKGVIIGVANDKSLAWGIAKAVCAQGAEVALTYLSETFKKRVDPLAESLGVKLTVPCDVSDAESVDNMFKV
LAEEWGSLDFVVHAVAFSDKNELKGRYVDTSLGNFLTSMHISCYSFTYIASKAEPLMTNGGSILTLSYYGAEKVVPHYNV
MGVCKAALEASVKYLAVDLGKQQIRVNAISAGPVRTLASSGISDFHYILTWNKYNSPLRRNTTLDDVGGAALYLLSDLGR
GTTGETVHVDCGYHVVGMKSVDAPDISRVKGDHSV

Sequences:

>Translated_275_residues
MRTGMLMEGKKGVIIGVANDKSLAWGIAKAVCAQGAEVALTYLSETFKKRVDPLAESLGVKLTVPCDVSDAESVDNMFKV
LAEEWGSLDFVVHAVAFSDKNELKGRYVDTSLGNFLTSMHISCYSFTYIASKAEPLMTNGGSILTLSYYGAEKVVPHYNV
MGVCKAALEASVKYLAVDLGKQQIRVNAISAGPVRTLASSGISDFHYILTWNKYNSPLRRNTTLDDVGGAALYLLSDLGR
GTTGETVHVDCGYHVVGMKSVDAPDISRVKGDHSV
>Mature_275_residues
MRTGMLMEGKKGVIIGVANDKSLAWGIAKAVCAQGAEVALTYLSETFKKRVDPLAESLGVKLTVPCDVSDAESVDNMFKV
LAEEWGSLDFVVHAVAFSDKNELKGRYVDTSLGNFLTSMHISCYSFTYIASKAEPLMTNGGSILTLSYYGAEKVVPHYNV
MGVCKAALEASVKYLAVDLGKQQIRVNAISAGPVRTLASSGISDFHYILTWNKYNSPLRRNTTLDDVGGAALYLLSDLGR
GTTGETVHVDCGYHVVGMKSVDAPDISRVKGDHSV

Specific function: Fatty acid biosynthesis pathway; second reduction step. [C]

COG id: COG0623

COG function: function code I; Enoyl-[acyl-carrier-protein] reductase (NADH)

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787545, Length=258, Percent_Identity=43.0232558139535, Blast_Score=230, Evalue=7e-62,
Organism=Escherichia coli, GI1789378, Length=259, Percent_Identity=26.2548262548263, Blast_Score=68, Evalue=7e-13,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR014358
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.3.1.9 [H]

Molecular weight: Translated: 29586; Mature: 29586

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTGMLMEGKKGVIIGVANDKSLAWGIAKAVCAQGAEVALTYLSETFKKRVDPLAESLGV
CCCCCEEECCCEEEEEEECCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCC
KLTVPCDVSDAESVDNMFKVLAEEWGSLDFVVHAVAFSDKNELKGRYVDTSLGNFLTSMH
EEEECCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEEECCCHHCCCEEECHHHHHHHHHHH
ISCYSFTYIASKAEPLMTNGGSILTLSYYGAEKVVPHYNVMGVCKAALEASVKYLAVDLG
HHHEEEEEECCCCCCEEECCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHEEEEECC
KQQIRVNAISAGPVRTLASSGISDFHYILTWNKYNSPLRRNTTLDDVGGAALYLLSDLGR
CCEEEEEEECCCCHHHHHHCCCCCEEEEEEECCCCCCCCCCCCHHHCCCHHHHHHHHCCC
GTTGETVHVDCGYHVVGMKSVDAPDISRVKGDHSV
CCCCCEEEEECCEEEEEECCCCCCCHHHCCCCCCC
>Mature Secondary Structure
MRTGMLMEGKKGVIIGVANDKSLAWGIAKAVCAQGAEVALTYLSETFKKRVDPLAESLGV
CCCCCEEECCCEEEEEEECCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCC
KLTVPCDVSDAESVDNMFKVLAEEWGSLDFVVHAVAFSDKNELKGRYVDTSLGNFLTSMH
EEEECCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEEECCCHHCCCEEECHHHHHHHHHHH
ISCYSFTYIASKAEPLMTNGGSILTLSYYGAEKVVPHYNVMGVCKAALEASVKYLAVDLG
HHHEEEEEECCCCCCEEECCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHEEEEECC
KQQIRVNAISAGPVRTLASSGISDFHYILTWNKYNSPLRRNTTLDDVGGAALYLLSDLGR
CCEEEEEEECCCCHHHHHHCCCCCEEEEEEECCCCCCCCCCCCHHHCCCHHHHHHHHCCC
GTTGETVHVDCGYHVVGMKSVDAPDISRVKGDHSV
CCCCCEEEEECCEEEEEECCCCCCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]