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The map label for this gene is gatB

Identifier: 88606922

GI number: 88606922

Start: 497496

End: 498947

Strand: Direct

Name: gatB

Synonym: APH_0470

Alternate gene names: 88606922

Gene position: 497496-498947 (Clockwise)

Preceding gene: 88607025

Following gene: 88607806

Centisome position: 33.81

GC content: 43.66

Gene sequence:

>1452_bases
GTGTCTTGGATAATCAACGGTGCAAGCCATGATTGGGAGGTTGTAATTGGTCTTGAGGTACACGCACAGATTGTATCGAA
TAGTAAATTATTTTCGGGGGCCTCTACGGAGATTTCTGTAGATCCAAATTCGCACGTGGCATTGTTAGATGTGGCGATGC
CGGGTGTTCTGCCAGTAACTAATGAGTATTGCATTGTTCAGGCGGTAAAAACAGCGCTTGCGCTTTCTTGCGAGGTGAAG
AAATATTCAGTATTTGATCGAAAGAATTACTTTTATCCTGATCTTTCTTTTGGATATCAAATTACGCAATTTTACCATCC
TATAGCTAAAAATGGCCACATAATGCTCGATGAATCTGCTGATAATAAGAAGATCGGCATTGCAAGGATTCATATTGAGC
AAGATGCTGGAAAGAGCCTGCACGTTGGTGATAAGACGTGTATAGACTTTAATAGGGCAGGGGTTGCTCTGATGGAGATT
GTTACAGATCCGGATTTACGGTCTCCGGAGGAGGCTGCCGAATATCTTAAGAAGCTGCGTATTATCTTAAGGTACATTGG
TGCGTGTAGTGGTGATATGGAAAACGGTGCCTTGCGCTGTGATGCAAATGTTTCGGTACGTAAGGTAGGTGAGAGTGCGT
TAGGAGTCCGCTCTGAGATCAAAAACTTGAATTCCATAAAGTATGTGGCTCAGGCTATAAAATACGAGGCTATGAGACAC
GTTGAAGTGCTGGAAAGCGGTGGTAAAGTGGAGCAAAGCACTCTTTTGTATGATGTTGACACTGGTACTACTAGGACTAT
GAGAAGTAAGGAGGATGTGTGTGATTATCGCTATTTTCCAGATCCTGACTTGCTTCCTTTGGAGATAACGGATGAGTTTA
TTGCGTCAATAAGAGATACGCTGCCTGAGCTTCCTATGGCGAAGATGTCGCGGTACATGAATGATTTTTCTCTTTCGAGG
TATGATGCAGTGATTCTCTCTTCTGATAGGGACGTTGCAGAATACTACGATGAGGTTATGCAGCGCAATTTGCCAGGGGA
CTTAGTTGCGGCATGGGTTACGGGTGAGCTTTTTGGAATGCTAAATAAGCGAGGCATGTGCATTAGTGATTCACCGGTAC
GTGCAGATGCCTTGGGAGATTTGCTGCGCTTAATGGTAGATGGGACTATTTCTGGCAAAATGGCTAAGCAGGTTTTTGCA
ACAATGTTTGAGACAGGAAAGTCAGCATCGAAGATAGTGGAAGAAGAAGGGTTACAGCAGATAGCAGATGAAGGGGTGCT
TAGTGCTATGGTTGAGCGAGTACTTGAGAAAAACCCAGATAAAGTTCAGCAATATAAGCAGGGTAAGGAAAAGCTATTTG
GATATTTTGTAGGACAGATAATGCAGGAAACAAAGGGCAGAGCGAATCCTGAAGTTCTCAATGCACTCATTCAGAGCAAG
CTGAGTCAGTAG

Upstream 100 bases:

>100_bases
AATCATGAGGCCTATGTTGTGCATGGGAAGGTCACATATTCTAGAGAAATAGCTACTCTTTTGAAGAAAAGTTGAGAAAA
GACAAATGTAGGAGGGCGTG

Downstream 100 bases:

>100_bases
TAGTATTTTACTCTATTGATAAGCACTTTCGGGCTTGTTGTGTCTAGTGAAATGTGGAGTACTGGGAGATAGGCTCTAAT
TCCTATGTGCTTTTGTAGAT

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B

Number of amino acids: Translated: 483; Mature: 482

Protein sequence:

>483_residues
MSWIINGASHDWEVVIGLEVHAQIVSNSKLFSGASTEISVDPNSHVALLDVAMPGVLPVTNEYCIVQAVKTALALSCEVK
KYSVFDRKNYFYPDLSFGYQITQFYHPIAKNGHIMLDESADNKKIGIARIHIEQDAGKSLHVGDKTCIDFNRAGVALMEI
VTDPDLRSPEEAAEYLKKLRIILRYIGACSGDMENGALRCDANVSVRKVGESALGVRSEIKNLNSIKYVAQAIKYEAMRH
VEVLESGGKVEQSTLLYDVDTGTTRTMRSKEDVCDYRYFPDPDLLPLEITDEFIASIRDTLPELPMAKMSRYMNDFSLSR
YDAVILSSDRDVAEYYDEVMQRNLPGDLVAAWVTGELFGMLNKRGMCISDSPVRADALGDLLRLMVDGTISGKMAKQVFA
TMFETGKSASKIVEEEGLQQIADEGVLSAMVERVLEKNPDKVQQYKQGKEKLFGYFVGQIMQETKGRANPEVLNALIQSK
LSQ

Sequences:

>Translated_483_residues
MSWIINGASHDWEVVIGLEVHAQIVSNSKLFSGASTEISVDPNSHVALLDVAMPGVLPVTNEYCIVQAVKTALALSCEVK
KYSVFDRKNYFYPDLSFGYQITQFYHPIAKNGHIMLDESADNKKIGIARIHIEQDAGKSLHVGDKTCIDFNRAGVALMEI
VTDPDLRSPEEAAEYLKKLRIILRYIGACSGDMENGALRCDANVSVRKVGESALGVRSEIKNLNSIKYVAQAIKYEAMRH
VEVLESGGKVEQSTLLYDVDTGTTRTMRSKEDVCDYRYFPDPDLLPLEITDEFIASIRDTLPELPMAKMSRYMNDFSLSR
YDAVILSSDRDVAEYYDEVMQRNLPGDLVAAWVTGELFGMLNKRGMCISDSPVRADALGDLLRLMVDGTISGKMAKQVFA
TMFETGKSASKIVEEEGLQQIADEGVLSAMVERVLEKNPDKVQQYKQGKEKLFGYFVGQIMQETKGRANPEVLNALIQSK
LSQ
>Mature_482_residues
SWIINGASHDWEVVIGLEVHAQIVSNSKLFSGASTEISVDPNSHVALLDVAMPGVLPVTNEYCIVQAVKTALALSCEVKK
YSVFDRKNYFYPDLSFGYQITQFYHPIAKNGHIMLDESADNKKIGIARIHIEQDAGKSLHVGDKTCIDFNRAGVALMEIV
TDPDLRSPEEAAEYLKKLRIILRYIGACSGDMENGALRCDANVSVRKVGESALGVRSEIKNLNSIKYVAQAIKYEAMRHV
EVLESGGKVEQSTLLYDVDTGTTRTMRSKEDVCDYRYFPDPDLLPLEITDEFIASIRDTLPELPMAKMSRYMNDFSLSRY
DAVILSSDRDVAEYYDEVMQRNLPGDLVAAWVTGELFGMLNKRGMCISDSPVRADALGDLLRLMVDGTISGKMAKQVFAT
MFETGKSASKIVEEEGLQQIADEGVLSAMVERVLEKNPDKVQQYKQGKEKLFGYFVGQIMQETKGRANPEVLNALIQSKL
SQ

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily

Homologues:

Organism=Homo sapiens, GI4758894, Length=498, Percent_Identity=39.9598393574297, Blast_Score=356, Evalue=3e-98,
Organism=Caenorhabditis elegans, GI115534094, Length=299, Percent_Identity=41.8060200668896, Blast_Score=226, Evalue=2e-59,
Organism=Saccharomyces cerevisiae, GI6319391, Length=507, Percent_Identity=31.9526627218935, Blast_Score=234, Evalue=3e-62,
Organism=Drosophila melanogaster, GI45550808, Length=486, Percent_Identity=35.59670781893, Blast_Score=313, Evalue=2e-85,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATB_ANAPZ (Q2GKN0)

Other databases:

- EMBL:   CP000235
- RefSeq:   YP_505071.1
- ProteinModelPortal:   Q2GKN0
- SMR:   Q2GKN0
- STRING:   Q2GKN0
- GeneID:   3930063
- GenomeReviews:   CP000235_GR
- KEGG:   aph:APH_0470
- NMPDR:   fig|212042.5.peg.465
- TIGR:   APH_0470
- eggNOG:   COG0064
- HOGENOM:   HBG395951
- OMA:   LRYLGTC
- PhylomeDB:   Q2GKN0
- ProtClustDB:   PRK05477
- BioCyc:   APHA212042:APH_0470-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00121
- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958
- PANTHER:   PTHR11659
- SMART:   SM00845
- TIGRFAMs:   TIGR00133

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey; SSF89095 GatB_Yqey

EC number: NA

Molecular weight: Translated: 53750; Mature: 53619

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSWIINGASHDWEVVIGLEVHAQIVSNSKLFSGASTEISVDPNSHVALLDVAMPGVLPVT
CCEEECCCCCCEEEEEEEEEEEHHHCCCCEECCCCCEEEECCCCCEEEEEECCCCCCCCC
NEYCIVQAVKTALALSCEVKKYSVFDRKNYFYPDLSFGYQITQFYHPIAKNGHIMLDESA
CCHHHHHHHHHHHHHHHCCHHHEEECCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEECCC
DNKKIGIARIHIEQDAGKSLHVGDKTCIDFNRAGVALMEIVTDPDLRSPEEAAEYLKKLR
CCCEEEEEEEEEECCCCCEEECCCCCEEECCCCCHHHHHHHCCCCCCCHHHHHHHHHHHH
IILRYIGACSGDMENGALRCDANVSVRKVGESALGVRSEIKNLNSIKYVAQAIKYEAMRH
HHHHHHHCCCCCCCCCEEEECCCCHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VEVLESGGKVEQSTLLYDVDTGTTRTMRSKEDVCDYRYFPDPDLLPLEITDEFIASIRDT
HHHHHCCCCEECCEEEEECCCCCCCCCCCHHHHHCCCCCCCCCCEEEEHHHHHHHHHHHH
LPELPMAKMSRYMNDFSLSRYDAVILSSDRDVAEYYDEVMQRNLPGDLVAAWVTGELFGM
CCCCCHHHHHHHHHHCCCCHHCEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
LNKRGMCISDSPVRADALGDLLRLMVDGTISGKMAKQVFATMFETGKSASKIVEEEGLQQ
HCCCCCEECCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
IADEGVLSAMVERVLEKNPDKVQQYKQGKEKLFGYFVGQIMQETKGRANPEVLNALIQSK
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
LSQ
HCC
>Mature Secondary Structure 
SWIINGASHDWEVVIGLEVHAQIVSNSKLFSGASTEISVDPNSHVALLDVAMPGVLPVT
CEEECCCCCCEEEEEEEEEEEHHHCCCCEECCCCCEEEECCCCCEEEEEECCCCCCCCC
NEYCIVQAVKTALALSCEVKKYSVFDRKNYFYPDLSFGYQITQFYHPIAKNGHIMLDESA
CCHHHHHHHHHHHHHHHCCHHHEEECCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEECCC
DNKKIGIARIHIEQDAGKSLHVGDKTCIDFNRAGVALMEIVTDPDLRSPEEAAEYLKKLR
CCCEEEEEEEEEECCCCCEEECCCCCEEECCCCCHHHHHHHCCCCCCCHHHHHHHHHHHH
IILRYIGACSGDMENGALRCDANVSVRKVGESALGVRSEIKNLNSIKYVAQAIKYEAMRH
HHHHHHHCCCCCCCCCEEEECCCCHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VEVLESGGKVEQSTLLYDVDTGTTRTMRSKEDVCDYRYFPDPDLLPLEITDEFIASIRDT
HHHHHCCCCEECCEEEEECCCCCCCCCCCHHHHHCCCCCCCCCCEEEEHHHHHHHHHHHH
LPELPMAKMSRYMNDFSLSRYDAVILSSDRDVAEYYDEVMQRNLPGDLVAAWVTGELFGM
CCCCCHHHHHHHHHHCCCCHHCEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
LNKRGMCISDSPVRADALGDLLRLMVDGTISGKMAKQVFATMFETGKSASKIVEEEGLQQ
HCCCCCEECCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
IADEGVLSAMVERVLEKNPDKVQQYKQGKEKLFGYFVGQIMQETKGRANPEVLNALIQSK
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
LSQ
HCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA