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The map label for this gene is nuoM [H]

Identifier: 88607159

GI number: 88607159

Start: 454433

End: 455908

Strand: Direct

Name: nuoM [H]

Synonym: APH_0438

Alternate gene names: 88607159

Gene position: 454433-455908 (Clockwise)

Preceding gene: 88607720

Following gene: 88606884

Centisome position: 30.89

GC content: 42.21

Gene sequence:

>1476_bases
GTGATCCCTTTTATGATTCTTATCCCCGTATTAGGGGGATGCCTTATTGCTTTTTTGAGAGACTCTTCCTCTAAGTTGAG
GGGCGGGCTATTTATTTCAATTACTTTTGCAACGTTCATAGCGAGTATCGCGGTTCTGCTGTCTTTCTATGGCAACGGGC
TTCCAGTATTAAGAGATTATACATGGATAGGAGAGGTATTTGCGGTAGATAGGTTATCATTGTTGATGGTGATACTATCG
ACGTTTCTCTTCTTTTTATGTGCATTATGTGGAGTATTCAGTCGTGCCTGTGGTGTTGATAAAGAATCAGAAAGCTCCTT
TTTTGCACTGTTGTTATTGCTAGAGGCGACGGTTCTTGGCGTCTTTTCTGTGCACACTTCTCTGATGTTTTATGTATTTT
TCGAAGCTTCGCTTATTCCTATGTTCTTTATAATAGGCAAGTGGGGCCATGGAGATCGGATCAGTGCTGCATTTAGGTTT
TTAATCTATACTGGAGCGGCATCTCTGTTATTTCTGATAGGGCTGGTATATTCGCGAGTTTTTTCTCTGGGGATGTTTGA
GTCTTTTTACGCAGATGTTTCTGTTGCGCTAGCTTTACCTATAGGTATTAGAATAGCGCTCTGGATTGCCTGCTTTTTTG
CATTTGCGGTGAAGCTTCCTATGGTTCCTTGTCATACTTGGTTGCCTAAGGCCCATGTACAGGCTCCTACTCTGGGTTCT
GTGTTGCTTGCTGGGTTGCTTATCAAAATGGGGGGGTACGGGTTTATAAGGTTCTGCCTTAGTGCACTTCCTGAAATCAG
CTGGAAGCTCTCGGAGTTTGTGGTTTATTTGAGCGCGATTTCACTGGTTTACTCGTCGTTAGTGGCTTTTGCCCAGAAGA
ATATGAAGACTCTAGTTGCATACTCTTCTGTAGCGCATATGAGCTTTGTTGCTGCTGGTATATTTTCACTAAATGAGAGT
GGAATTGTTGGTGCAATTTTTCAAATGCTCAGTCATGGTCTTATTTCAGCTGCGCTGTTTTTATGTGTTGGTATGATATA
TAGCCGTACTGGTACTATGGATATGTCTAAGTGTGGTGGGATAGCTGCATCGATGCCCAAGCTTTCATTTATGATGATAT
TCTTTAGCATGGCTTCAGCTGGTTTGCCGGGAACCTCAGGTTTTATCGGGGAGTTTCTATCCATATTGGGAATTTTTCAG
GCTTTTAGGTTTACTGCAGTTTGTTTCGCGGTTGGAGTGGTATTGAGCGCTGCTTACACGTTGCGTTTGTGTCGTGAAGT
GATATGGGGAAGTAGCCCTGAGGGTGCTAAGGTGATAGATAGTGACATTAACGCTGTTGAATTTGTTATTTTGGGGATAT
TGGCAGCTCTTGTTCTATTCTTTGGTATATATCCTGCTTCCGTGATAACATTTTTAAAGCCTTGGATAAGTGCTATTTTG
CCAGAATTACAAAAGTTTCAATTGTCTTGGGGGTGA

Upstream 100 bases:

>100_bases
GTGTAAGAATACAGAGTGGACGTGTAGTTGGCTATGCTCTTGTAATGCTGCTTGGAGTGGTATCTGCTGCGCTGTTAGTG
GTATATGGTTTTAAAACACA

Downstream 100 bases:

>100_bases
TGTGTATTTGAGCGACTTTCTATACATAGTGCCCGAGATTACTGTATTGGGTTCTGCTCTAGCGCTACTGGTGTTGGGGA
TGTTTACGAGTGAGCGGCGA

Product: NADH dehydrogenase I, M subunit

Products: NA

Alternate protein names: NADH dehydrogenase I subunit M; NDH-1 subunit M [H]

Number of amino acids: Translated: 491; Mature: 491

Protein sequence:

>491_residues
MIPFMILIPVLGGCLIAFLRDSSSKLRGGLFISITFATFIASIAVLLSFYGNGLPVLRDYTWIGEVFAVDRLSLLMVILS
TFLFFLCALCGVFSRACGVDKESESSFFALLLLLEATVLGVFSVHTSLMFYVFFEASLIPMFFIIGKWGHGDRISAAFRF
LIYTGAASLLFLIGLVYSRVFSLGMFESFYADVSVALALPIGIRIALWIACFFAFAVKLPMVPCHTWLPKAHVQAPTLGS
VLLAGLLIKMGGYGFIRFCLSALPEISWKLSEFVVYLSAISLVYSSLVAFAQKNMKTLVAYSSVAHMSFVAAGIFSLNES
GIVGAIFQMLSHGLISAALFLCVGMIYSRTGTMDMSKCGGIAASMPKLSFMMIFFSMASAGLPGTSGFIGEFLSILGIFQ
AFRFTAVCFAVGVVLSAAYTLRLCREVIWGSSPEGAKVIDSDINAVEFVILGILAALVLFFGIYPASVITFLKPWISAIL
PELQKFQLSWG

Sequences:

>Translated_491_residues
MIPFMILIPVLGGCLIAFLRDSSSKLRGGLFISITFATFIASIAVLLSFYGNGLPVLRDYTWIGEVFAVDRLSLLMVILS
TFLFFLCALCGVFSRACGVDKESESSFFALLLLLEATVLGVFSVHTSLMFYVFFEASLIPMFFIIGKWGHGDRISAAFRF
LIYTGAASLLFLIGLVYSRVFSLGMFESFYADVSVALALPIGIRIALWIACFFAFAVKLPMVPCHTWLPKAHVQAPTLGS
VLLAGLLIKMGGYGFIRFCLSALPEISWKLSEFVVYLSAISLVYSSLVAFAQKNMKTLVAYSSVAHMSFVAAGIFSLNES
GIVGAIFQMLSHGLISAALFLCVGMIYSRTGTMDMSKCGGIAASMPKLSFMMIFFSMASAGLPGTSGFIGEFLSILGIFQ
AFRFTAVCFAVGVVLSAAYTLRLCREVIWGSSPEGAKVIDSDINAVEFVILGILAALVLFFGIYPASVITFLKPWISAIL
PELQKFQLSWG
>Mature_491_residues
MIPFMILIPVLGGCLIAFLRDSSSKLRGGLFISITFATFIASIAVLLSFYGNGLPVLRDYTWIGEVFAVDRLSLLMVILS
TFLFFLCALCGVFSRACGVDKESESSFFALLLLLEATVLGVFSVHTSLMFYVFFEASLIPMFFIIGKWGHGDRISAAFRF
LIYTGAASLLFLIGLVYSRVFSLGMFESFYADVSVALALPIGIRIALWIACFFAFAVKLPMVPCHTWLPKAHVQAPTLGS
VLLAGLLIKMGGYGFIRFCLSALPEISWKLSEFVVYLSAISLVYSSLVAFAQKNMKTLVAYSSVAHMSFVAAGIFSLNES
GIVGAIFQMLSHGLISAALFLCVGMIYSRTGTMDMSKCGGIAASMPKLSFMMIFFSMASAGLPGTSGFIGEFLSILGIFQ
AFRFTAVCFAVGVVLSAAYTLRLCREVIWGSSPEGAKVIDSDINAVEFVILGILAALVLFFGIYPASVITFLKPWISAIL
PELQKFQLSWG

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1008

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 4 (chain M)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 4 family [H]

Homologues:

Organism=Homo sapiens, GI251831116, Length=326, Percent_Identity=32.8220858895706, Blast_Score=170, Evalue=3e-42,
Organism=Homo sapiens, GI251831117, Length=375, Percent_Identity=23.7333333333333, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI1788613, Length=458, Percent_Identity=35.1528384279476, Blast_Score=244, Evalue=7e-66,
Organism=Escherichia coli, GI1788827, Length=308, Percent_Identity=29.5454545454545, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1788831, Length=376, Percent_Identity=26.063829787234, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI2367154, Length=269, Percent_Identity=27.8810408921933, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI145693160, Length=394, Percent_Identity=26.1421319796954, Blast_Score=66, Evalue=5e-12,
Organism=Escherichia coli, GI1788829, Length=408, Percent_Identity=22.3039215686275, Blast_Score=66, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010227
- InterPro:   IPR001750
- InterPro:   IPR003918
- InterPro:   IPR000260 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF01059 Oxidored_q5_N [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 53289; Mature: 53289

Theoretical pI: Translated: 8.34; Mature: 8.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHCCCC
>Mature Secondary Structure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HHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8566820 [H]