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The map label for this gene is nuoN

Identifier: 88606884

GI number: 88606884

Start: 455910

End: 457304

Strand: Direct

Name: nuoN

Synonym: APH_0439

Alternate gene names: 88606884

Gene position: 455910-457304 (Clockwise)

Preceding gene: 88607159

Following gene: 88607594

Centisome position: 30.99

GC content: 43.3

Gene sequence:

>1395_bases
GTGTATTTGAGCGACTTTCTATACATAGTGCCCGAGATTACTGTATTGGGTTCTGCTCTAGCGCTACTGGTGTTGGGGAT
GTTTACGAGTGAGCGGCGAGTGCGGAGTATGTCTCTTATCTCTGTTGCTATAGCGGCTGTGTTGGCGTGTAAGGAGTTAA
TATATTTTTCTGGAGAAGAGGTATCTCTTTTTGGTGGCTTCGTGGTGCGTACTGCGCATACCTGTCTTGCTAGGGCAGTA
GTTGCAGTATCGGGGCTTTTTGCCTTCTTGTTATTTTTCTTTGCGAAGCGTAGTTACCGCTATGAGTTTGCTGTTTTGAT
GCTTTTTGCTTTCTTGGGAACTCTGACTCTTGTCGAAGCGCATCATTTCCTGTCTTTTTATCTTTCTTTTGAGCTGATAG
GCTTTGCTTCTTACATATTGGTATGCTTTAATCGCAGCTCTATAAAGGCGTCAGAGGCTGCTATCAAGTTTTTTGTTCTA
GGTGCTCTCTCATCGTGCATAATGCTATATGGTATTTCCTTAGTATATGGATATGCTTCGGAATTTAGTCTTGGTGTTGT
TTCTAAAGTGTTGGGAGGAGAGGAATCTCTAGGGGCAACTTTTGGCTGTGCTTTAGTGCTTGTGGGGTTATTGTTCAAGC
TTGGTGCTGTTCCGTTTCATATGTGGATTCCCGATACGTATGAAGGTGCGCCAACTGTTGCGGTGGTGTTTTTTACTATA
GTTACTAAAACAGCTATGGTATTAGTCTTTGCAGGATTAATGCAGGGGGTTGTTATTCCTATTACGGGTTTTGTATGGAG
CATGTTGTTGATGGCTGCATTATCGATGGTGGTGGGTGAGTTTTCTGCTATGCAGCAGAAGAATGTTAAGAGGCTGTTTG
CGTATGCTAACATTGGCCATATTGGTTATGTATTAGCCGGCATGAGTACAGGAGTTGTTACTTTCAAGCCTGTGCTGTTT
TATGTGGTGACATATCTGCTCATTAATGTATGGATTTTCACGGTGCTGCTTAGGTATGATGACGAGGGGTTTGAGATAAC
AGATGTTGCTGGTTTAGCCGCAAAAAATCCGTTTTTGGCATTTACGTTTGTGGCAGCGTTGTTGGCGTCGGCTGGGTTGC
CACCGTTTTCTGGTTTTTTTGCTAAGTACACATTACTTAAGGCGATAGGTGGTGTTGATGCATTTGGGGTGCCGACGCTT
GTATGCGTAGTATTCCTATGTCTCACTAGTATTATCCCGTGTTTTTATTGTTTTCGCATAGCCAAGGTGGTTTATTTTGA
TGTGCCGACGGGGGAACATTCCGCTACAAGCAGGAATGTTGGTTTGTCGATTATGGCATTTGTCGCTGTAACACTTAGTC
TTGTAGTTGTGCTTCTAAGAGAGCGTATTATATAG

Upstream 100 bases:

>100_bases
TTCTTTGGTATATATCCTGCTTCCGTGATAACATTTTTAAAGCCTTGGATAAGTGCTATTTTGCCAGAATTACAAAAGTT
TCAATTGTCTTGGGGGTGAT

Downstream 100 bases:

>100_bases
CTACTCTGAGCTAATCTCTGCATAAGCAAGTAAGCAGTAGGAAGATCATGTGTTGTAAGAGTTACTAGTGGAGTTTTTTG
TATACGGTAGAGGTGTGTGA

Product: NADH dehydrogenase I, N subunit

Products: NA

Alternate protein names: NADH dehydrogenase I subunit N; NDH-1 subunit N

Number of amino acids: Translated: 464; Mature: 464

Protein sequence:

>464_residues
MYLSDFLYIVPEITVLGSALALLVLGMFTSERRVRSMSLISVAIAAVLACKELIYFSGEEVSLFGGFVVRTAHTCLARAV
VAVSGLFAFLLFFFAKRSYRYEFAVLMLFAFLGTLTLVEAHHFLSFYLSFELIGFASYILVCFNRSSIKASEAAIKFFVL
GALSSCIMLYGISLVYGYASEFSLGVVSKVLGGEESLGATFGCALVLVGLLFKLGAVPFHMWIPDTYEGAPTVAVVFFTI
VTKTAMVLVFAGLMQGVVIPITGFVWSMLLMAALSMVVGEFSAMQQKNVKRLFAYANIGHIGYVLAGMSTGVVTFKPVLF
YVVTYLLINVWIFTVLLRYDDEGFEITDVAGLAAKNPFLAFTFVAALLASAGLPPFSGFFAKYTLLKAIGGVDAFGVPTL
VCVVFLCLTSIIPCFYCFRIAKVVYFDVPTGEHSATSRNVGLSIMAFVAVTLSLVVVLLRERII

Sequences:

>Translated_464_residues
MYLSDFLYIVPEITVLGSALALLVLGMFTSERRVRSMSLISVAIAAVLACKELIYFSGEEVSLFGGFVVRTAHTCLARAV
VAVSGLFAFLLFFFAKRSYRYEFAVLMLFAFLGTLTLVEAHHFLSFYLSFELIGFASYILVCFNRSSIKASEAAIKFFVL
GALSSCIMLYGISLVYGYASEFSLGVVSKVLGGEESLGATFGCALVLVGLLFKLGAVPFHMWIPDTYEGAPTVAVVFFTI
VTKTAMVLVFAGLMQGVVIPITGFVWSMLLMAALSMVVGEFSAMQQKNVKRLFAYANIGHIGYVLAGMSTGVVTFKPVLF
YVVTYLLINVWIFTVLLRYDDEGFEITDVAGLAAKNPFLAFTFVAALLASAGLPPFSGFFAKYTLLKAIGGVDAFGVPTL
VCVVFLCLTSIIPCFYCFRIAKVVYFDVPTGEHSATSRNVGLSIMAFVAVTLSLVVVLLRERII
>Mature_464_residues
MYLSDFLYIVPEITVLGSALALLVLGMFTSERRVRSMSLISVAIAAVLACKELIYFSGEEVSLFGGFVVRTAHTCLARAV
VAVSGLFAFLLFFFAKRSYRYEFAVLMLFAFLGTLTLVEAHHFLSFYLSFELIGFASYILVCFNRSSIKASEAAIKFFVL
GALSSCIMLYGISLVYGYASEFSLGVVSKVLGGEESLGATFGCALVLVGLLFKLGAVPFHMWIPDTYEGAPTVAVVFFTI
VTKTAMVLVFAGLMQGVVIPITGFVWSMLLMAALSMVVGEFSAMQQKNVKRLFAYANIGHIGYVLAGMSTGVVTFKPVLF
YVVTYLLINVWIFTVLLRYDDEGFEITDVAGLAAKNPFLAFTFVAALLASAGLPPFSGFFAKYTLLKAIGGVDAFGVPTL
VCVVFLCLTSIIPCFYCFRIAKVVYFDVPTGEHSATSRNVGLSIMAFVAVTLSLVVVLLRERII

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1007

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 2 (chain N)

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 2 family

Homologues:

Organism=Homo sapiens, GI251831108, Length=309, Percent_Identity=19.4174757281553, Blast_Score=69, Evalue=7e-12,
Organism=Escherichia coli, GI145693160, Length=381, Percent_Identity=30.9711286089239, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI1788831, Length=305, Percent_Identity=26.8852459016393, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1788829, Length=294, Percent_Identity=25.5102040816327, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1788614, Length=317, Percent_Identity=23.9747634069401, Blast_Score=69, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NUON_ANAPZ (Q2GKQ9)

Other databases:

- EMBL:   CP000235
- RefSeq:   YP_505042.1
- STRING:   Q2GKQ9
- GeneID:   3930437
- GenomeReviews:   CP000235_GR
- KEGG:   aph:APH_0439
- NMPDR:   fig|212042.5.peg.428
- TIGR:   APH_0439
- HOGENOM:   HBG747830
- OMA:   SIGHIGF
- PhylomeDB:   Q2GKQ9
- ProtClustDB:   CLSK747351
- BioCyc:   APHA212042:APH_0439-MONOMER
- GO:   GO:0006810
- HAMAP:   MF_00445
- InterPro:   IPR010096
- InterPro:   IPR001750

Pfam domain/function: PF00361 Oxidored_q1

EC number: =1.6.99.5

Molecular weight: Translated: 50439; Mature: 50439

Theoretical pI: Translated: 8.12; Mature: 8.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x17896ae8)-; HASH(0x18b7ffcc)-; HASH(0x18b7feb8)-; HASH(0x1829d3e0)-; HASH(0x18b8014c)-; HASH(0x18738b34)-; HASH(0x1842de68)-; HASH(0x18b7ff30)-; HASH(0x17b2fa74)-; HASH(0x18b80020)-; HASH(0x170fdac4)-; HASH(0x1842e0cc)-; HASH(0x1885b308)-; HASH(0x18b7feac)-;

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYLSDFLYIVPEITVLGSALALLVLGMFTSERRVRSMSLISVAIAAVLACKELIYFSGEE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
VSLFGGFVVRTAHTCLARAVVAVSGLFAFLLFFFAKRSYRYEFAVLMLFAFLGTLTLVEA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
HHFLSFYLSFELIGFASYILVCFNRSSIKASEAAIKFFVLGALSSCIMLYGISLVYGYAS
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EFSLGVVSKVLGGEESLGATFGCALVLVGLLFKLGAVPFHMWIPDTYEGAPTVAVVFFTI
HHHHHHHHHHHCCCHHCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHH
VTKTAMVLVFAGLMQGVVIPITGFVWSMLLMAALSMVVGEFSAMQQKNVKRLFAYANIGH
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
IGYVLAGMSTGVVTFKPVLFYVVTYLLINVWIFTVLLRYDDEGFEITDVAGLAAKNPFLA
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHCCCCHHH
FTFVAALLASAGLPPFSGFFAKYTLLKAIGGVDAFGVPTLVCVVFLCLTSIIPCFYCFRI
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
AKVVYFDVPTGEHSATSRNVGLSIMAFVAVTLSLVVVLLRERII
HHHHEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCH
>Mature Secondary Structure
MYLSDFLYIVPEITVLGSALALLVLGMFTSERRVRSMSLISVAIAAVLACKELIYFSGEE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
VSLFGGFVVRTAHTCLARAVVAVSGLFAFLLFFFAKRSYRYEFAVLMLFAFLGTLTLVEA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
HHFLSFYLSFELIGFASYILVCFNRSSIKASEAAIKFFVLGALSSCIMLYGISLVYGYAS
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EFSLGVVSKVLGGEESLGATFGCALVLVGLLFKLGAVPFHMWIPDTYEGAPTVAVVFFTI
HHHHHHHHHHHCCCHHCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHH
VTKTAMVLVFAGLMQGVVIPITGFVWSMLLMAALSMVVGEFSAMQQKNVKRLFAYANIGH
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
IGYVLAGMSTGVVTFKPVLFYVVTYLLINVWIFTVLLRYDDEGFEITDVAGLAAKNPFLA
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHCCCCHHH
FTFVAALLASAGLPPFSGFFAKYTLLKAIGGVDAFGVPTLVCVVFLCLTSIIPCFYCFRI
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
AKVVYFDVPTGEHSATSRNVGLSIMAFVAVTLSLVVVLLRERII
HHHHEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA