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The map label for this gene is nagP [H]

Identifier: 87200425

GI number: 87200425

Start: 2583756

End: 2585444

Strand: Reverse

Name: nagP [H]

Synonym: Saro_2412

Alternate gene names: 87200425

Gene position: 2585444-2583756 (Counterclockwise)

Preceding gene: 87200426

Following gene: 87200424

Centisome position: 72.59

GC content: 69.63

Gene sequence:

>1689_bases
ATGAGGAACCTTCTCGGCCTGCTCCAGCCGATCGGTCGCGCGCTGATGCTGCCGATCGCGGTGCTCCCCGTCGCGGGGCT
TCTGCTGCGCCTCGGCCAGCCCGACCTGCTCGATCTTCCGCTGCTGGCCGCGTCCGGCGATGCGCTCTTCTCCAGCCTCG
GACTGCTTTTCGCCATCGGCGTTGCCGCGGGCATTGCGCGCGACGGCAATGGTGCGGCGTGCCTCGCGGGGGTGGTCTGT
TATCTCGTCACGATGAACGGCGGCAAGGCGCTCCTGCCGGTCCCGACGGACGTCACGCTCGGCCTTGCCGATACGCTTGC
CCAGACCGTCGCAAGGGCGTGGAAGGCCAAGGCCTTTGCCCGGCTTGACGTACCCATCGGGATCGTCTCGGGTCTGCTCG
GCGGCGCGCTCTACAACCGCTTCTCGACCATCGCCGTTCCTGCCTATCTCGCCTTCTTCGGCGGAAGGCGTTTCGTGCCC
ATCGTCGCGGGTGGCGCGGGGGTCGTGCTTGCCGGGGTCGTGGGCCTTGGCTTCCCGGCACTCGATGCCGCGCTCGATCA
CGGTAGCCGGGGTCTCGTTGCCGCAGGTCCGGTCGGGCTCTTCGCCTTCGGGGTTCTGAACCGGCTGCTCCTCGTCACCG
GGCTCCACCACATCCTCAACAACGTCGCCTGGTTCGTCCTGGGCGATTTCGGGGGGACGACCGGCGACCTGCGCCGCTTC
TTTGCCGGAGACCCGCATGCGGGCGCCTTCATGGCCGGGTTCTTCCCGATCATGATGTTCGGCCTGCCCGCCGCCTGCCT
CGCCATGTACCGGGCCGCGTTGCCCGATCAGCGCAAGGCGACCGGCGGCATGCTTCTCAGCCTCGCGCTCACCTCGTTCC
TGACCGGCGTGACCGAGCCGATCGAGTTCAGCTTCATGTTCCTCGCACCGATGCTCTACGCGGTCCACGCGGTGCTGACG
GGCGCGGCGATGGTTCTGATGGACGTGCTCGGCGTGCGCATGGGCTTCGGCTTTTCCGCCGGCCTTTTCGACTATGTGCT
GAACTTCGGCCGAGCCACCCGGCCGCTTCTCCTGCTGCCCGTCGGCCTTGCCTATTTCGCGATCTACTACGCGGTCTTCA
GCTATGCGATCCGCCGTTTCGACCTCGCGACGCCAGGCCGCCAGCCGCTCGCGCCCTCCGGCCAGCAGGAAACCGGCACC
GACGGCGAGTTGGGCCACGCCTATGTCGAAGCCCTTGGCGGCGCCGCCAACATCGCCACCTTGGGCGCCTGCACCACGCG
CCTGCGCCTGGTCGTCCGCGATCCCGCGGCGGTCGACGATGCCGCGCTCAAGGCGCTCGGCGCGGTCGCGGTGCTGCGCC
CTGCCGCCGATGCCGTGCAGGTCATAATCGGCCCCATGGCAGACCGCATCTGCGCCGAGATGGCCGACGTCGTGAAGCAC
GCACCCGCCGCCCCCGCCGCCACGCCTGTCCGCTCCGAAGCGTTAGCCGTCAGCCTGCGCCCGGAAATCCTTGCCGCGCT
CGGCGGAGCCGATGCCGTCGTCCAGGCCTCGCGCGCGGCCGGACGCATCCGCGTGACGTTTCGCCGTGACGCGGATCTTG
CGGCAATGGCCCCGGTCCCCGGCCTGCGCTGCGTCGCCCGCATCGACGCGCGGACCTGGCACCTCATCGGCCAGGACCTC
GCGGTCTGA

Upstream 100 bases:

>100_bases
TGTCCATCTCGGACTGCAAGACTCTCGCCCTCGAGGCGCTTCAGCTTGAATCCGCTGCCGAAATCCGTGCCCTGTCGCGG
GCATTCGTGGAGACACTGCC

Downstream 100 bases:

>100_bases
GCCGCGCGGCGATGACGCCGACCGGCCTGCATCGCCGCGAGGTCCTGTCGCTGCTGGGCACGACAGCCTGTACCATCGCC
GCCTCTGCCCACGCTTCGGC

Product: PTS system, N-acetylglucosamine-specific IIBC component

Products: NA

Alternate protein names: EIICB-Nag; N-acetylglucosamine permease IIC component; PTS system N-acetylglucosamine-specific EIIC component; N-acetylglucosamine-specific phosphotransferase enzyme IIB component; PTS system N-acetylglucosamine-specific EIIB component [H]

Number of amino acids: Translated: 562; Mature: 562

Protein sequence:

>562_residues
MRNLLGLLQPIGRALMLPIAVLPVAGLLLRLGQPDLLDLPLLAASGDALFSSLGLLFAIGVAAGIARDGNGAACLAGVVC
YLVTMNGGKALLPVPTDVTLGLADTLAQTVARAWKAKAFARLDVPIGIVSGLLGGALYNRFSTIAVPAYLAFFGGRRFVP
IVAGGAGVVLAGVVGLGFPALDAALDHGSRGLVAAGPVGLFAFGVLNRLLLVTGLHHILNNVAWFVLGDFGGTTGDLRRF
FAGDPHAGAFMAGFFPIMMFGLPAACLAMYRAALPDQRKATGGMLLSLALTSFLTGVTEPIEFSFMFLAPMLYAVHAVLT
GAAMVLMDVLGVRMGFGFSAGLFDYVLNFGRATRPLLLLPVGLAYFAIYYAVFSYAIRRFDLATPGRQPLAPSGQQETGT
DGELGHAYVEALGGAANIATLGACTTRLRLVVRDPAAVDDAALKALGAVAVLRPAADAVQVIIGPMADRICAEMADVVKH
APAAPAATPVRSEALAVSLRPEILAALGGADAVVQASRAAGRIRVTFRRDADLAAMAPVPGLRCVARIDARTWHLIGQDL
AV

Sequences:

>Translated_562_residues
MRNLLGLLQPIGRALMLPIAVLPVAGLLLRLGQPDLLDLPLLAASGDALFSSLGLLFAIGVAAGIARDGNGAACLAGVVC
YLVTMNGGKALLPVPTDVTLGLADTLAQTVARAWKAKAFARLDVPIGIVSGLLGGALYNRFSTIAVPAYLAFFGGRRFVP
IVAGGAGVVLAGVVGLGFPALDAALDHGSRGLVAAGPVGLFAFGVLNRLLLVTGLHHILNNVAWFVLGDFGGTTGDLRRF
FAGDPHAGAFMAGFFPIMMFGLPAACLAMYRAALPDQRKATGGMLLSLALTSFLTGVTEPIEFSFMFLAPMLYAVHAVLT
GAAMVLMDVLGVRMGFGFSAGLFDYVLNFGRATRPLLLLPVGLAYFAIYYAVFSYAIRRFDLATPGRQPLAPSGQQETGT
DGELGHAYVEALGGAANIATLGACTTRLRLVVRDPAAVDDAALKALGAVAVLRPAADAVQVIIGPMADRICAEMADVVKH
APAAPAATPVRSEALAVSLRPEILAALGGADAVVQASRAAGRIRVTFRRDADLAAMAPVPGLRCVARIDARTWHLIGQDL
AV
>Mature_562_residues
MRNLLGLLQPIGRALMLPIAVLPVAGLLLRLGQPDLLDLPLLAASGDALFSSLGLLFAIGVAAGIARDGNGAACLAGVVC
YLVTMNGGKALLPVPTDVTLGLADTLAQTVARAWKAKAFARLDVPIGIVSGLLGGALYNRFSTIAVPAYLAFFGGRRFVP
IVAGGAGVVLAGVVGLGFPALDAALDHGSRGLVAAGPVGLFAFGVLNRLLLVTGLHHILNNVAWFVLGDFGGTTGDLRRF
FAGDPHAGAFMAGFFPIMMFGLPAACLAMYRAALPDQRKATGGMLLSLALTSFLTGVTEPIEFSFMFLAPMLYAVHAVLT
GAAMVLMDVLGVRMGFGFSAGLFDYVLNFGRATRPLLLLPVGLAYFAIYYAVFSYAIRRFDLATPGRQPLAPSGQQETGT
DGELGHAYVEALGGAANIATLGACTTRLRLVVRDPAAVDDAALKALGAVAVLRPAADAVQVIIGPMADRICAEMADVVKH
APAAPAATPVRSEALAVSLRPEILAALGGADAVVQASRAAGRIRVTFRRDADLAAMAPVPGLRCVARIDARTWHLIGQDL
AV

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1263

COG function: function code G; Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1786894, Length=496, Percent_Identity=47.1774193548387, Blast_Score=378, Evalue=1e-106,
Organism=Escherichia coli, GI1787343, Length=491, Percent_Identity=39.1038696537678, Blast_Score=316, Evalue=3e-87,
Organism=Escherichia coli, GI1787908, Length=537, Percent_Identity=34.8230912476723, Blast_Score=233, Evalue=3e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018113
- InterPro:   IPR001996
- InterPro:   IPR003352
- InterPro:   IPR013013
- InterPro:   IPR011535
- InterPro:   IPR010974 [H]

Pfam domain/function: PF00367 PTS_EIIB; PF02378 PTS_EIIC [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 58081; Mature: 58081

Theoretical pI: Translated: 8.97; Mature: 8.97

Prosite motif: PS01035 PTS_EIIB_TYPE_1_CYS ; PS51098 PTS_EIIB_TYPE_1 ; PS51103 PTS_EIIC_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNLLGLLQPIGRALMLPIAVLPVAGLLLRLGQPDLLDLPLLAASGDALFSSLGLLFAIG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCHHHHHHHHHHHHHH
VAAGIARDGNGAACLAGVVCYLVTMNGGKALLPVPTDVTLGLADTLAQTVARAWKAKAFA
HHHHHCCCCCCHHHHHHHHHHHHEECCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
RLDVPIGIVSGLLGGALYNRFSTIAVPAYLAFFGGRRFVPIVAGGAGVVLAGVVGLGFPA
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHCCCHH
LDAALDHGSRGLVAAGPVGLFAFGVLNRLLLVTGLHHILNNVAWFVLGDFGGTTGDLRRF
HHHHHCCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHH
FAGDPHAGAFMAGFFPIMMFGLPAACLAMYRAALPDQRKATGGMLLSLALTSFLTGVTEP
HCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCC
IEFSFMFLAPMLYAVHAVLTGAAMVLMDVLGVRMGFGFSAGLFDYVLNFGRATRPLLLLP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCHHHHH
VGLAYFAIYYAVFSYAIRRFDLATPGRQPLAPSGQQETGTDGELGHAYVEALGGAANIAT
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHH
LGACTTRLRLVVRDPAAVDDAALKALGAVAVLRPAADAVQVIIGPMADRICAEMADVVKH
HHHHHHHHEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
APAAPAATPVRSEALAVSLRPEILAALGGADAVVQASRAAGRIRVTFRRDADLAAMAPVP
CCCCCCCCCCCCHHEEEECCHHHHHHHCCHHHHHHHHHCCCEEEEEEECCCCCCCCCCCC
GLRCVARIDARTWHLIGQDLAV
CHHHHHHHCCHHHHHHHHHCCC
>Mature Secondary Structure
MRNLLGLLQPIGRALMLPIAVLPVAGLLLRLGQPDLLDLPLLAASGDALFSSLGLLFAIG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCHHHHHHHHHHHHHH
VAAGIARDGNGAACLAGVVCYLVTMNGGKALLPVPTDVTLGLADTLAQTVARAWKAKAFA
HHHHHCCCCCCHHHHHHHHHHHHEECCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
RLDVPIGIVSGLLGGALYNRFSTIAVPAYLAFFGGRRFVPIVAGGAGVVLAGVVGLGFPA
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHCCCHH
LDAALDHGSRGLVAAGPVGLFAFGVLNRLLLVTGLHHILNNVAWFVLGDFGGTTGDLRRF
HHHHHCCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHH
FAGDPHAGAFMAGFFPIMMFGLPAACLAMYRAALPDQRKATGGMLLSLALTSFLTGVTEP
HCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCC
IEFSFMFLAPMLYAVHAVLTGAAMVLMDVLGVRMGFGFSAGLFDYVLNFGRATRPLLLLP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCHHHHH
VGLAYFAIYYAVFSYAIRRFDLATPGRQPLAPSGQQETGTDGELGHAYVEALGGAANIAT
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHH
LGACTTRLRLVVRDPAAVDDAALKALGAVAVLRPAADAVQVIIGPMADRICAEMADVVKH
HHHHHHHHEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
APAAPAATPVRSEALAVSLRPEILAALGGADAVVQASRAAGRIRVTFRRDADLAAMAPVP
CCCCCCCCCCCCHHEEEECCHHHHHHHCCHHHHHHHHHCCCEEEEEEECCCCCCCCCCCC
GLRCVARIDARTWHLIGQDLAV
CHHHHHHHCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9272861; 9384377 [H]